SlideShare a Scribd company logo
ENCASED CANTILEVERS FOR LOW-NOISE FORCE
AND MASS SENSING IN LIQUIDS

Dominik Ziegler PhD
Material Science Division, Molecular Foundry, Lawrence Berkeley National Laboratory
High Resolution
Imaging of Mica

Encased Cantilevers

Fabrication
Quantitative Mass Sensor

Gentle Imaging of
Lipid Bilayers

Interferometric
Deflection Detection

Dominik Ziegler PhD
Material Science Division, Molecular Foundry, Lawrence Berkeley National Laboratory
VISCOUS DAMPING LIMITS RESOLUTION
Fn


4k BTk
0Qi

4k BTb

Force Noise  Smallest Measurable Force
ω0≈150 kHz
Q≈200

ω0≈50 kHz
Q≈4

Fn air≈15fN/√Hz

Fn water ≈200fN/√Hz

100 m long, 30 m wide



High forces deform soft samples and limits resolution
LET`S GO SCUBA DIVING!
FABRICATION OF ENCASED CANTILEVERS

-Length of levers can be tuned
-Tip’s sharpness is maintained
FABRICATION OF ENCASED CANTILEVERS

-Length of levers can be tuned
-Tip’s sharpness is maintained
ENCASED CANTILEVER IN AIR/WATER
air
water

fair=310.280kHz
fwater=309.71kHz
Qair=210
Qwater=133

Single clean resonance peak.

High Q and high frequency, performance as in air (Fn=12 fN/sqrt(Hz))
-Only small frequency shift (1%)  No Added Mass
-Viscous damping of tip (few um) and double sided squeeze film damping
GENTLE IMAGING OF LIPID BILAYERS
height of supported DPPC
bilayers on mica
softest possible imaging
amplitude modulation
“Hydra Levers” by AppNano
Encased silicon lever (Mikromasch NSC36)
L = 200 µm, k=0.084N/m f=17 kHz
l =110 µm, k = 0.95N/m, f=85kHz

Thicker measured height
 Less deformation by tip
HIGH-RESOLUTION IMAGING OF MICA IN LIQUID

0.5 nm

MICA lattice using amplitude modulation
300mM KCl, 10mM K2HPO4, 5 lines/s
Unmodified commercial instrument
Multimode, Bruker
OSCILLATORY HYDRATION FORCE
Amplitude[mV]
Amplitude [Å]

(WATER STRUCTURE ON MICA)

20
15
10
5
0

0

1

0

1

Phase [Deg]
Phase[Deg]

60

3

2

[nm]

2

3

0.5 nm

40
20
0

-20
[nm]

~4Å

Ultra-small amplitude
displacement of the last few water layers
single curve no averaging

Kobayashi, J. CHEM. PHYS. 138, 184704 (2013)
QUANTITATIVE MASS SENSING WITH ENCASED CANTILEVERS

Indirect measurement of mass over
surface stress (DC measurement)

0

Known location of added mass  quantitative mass sensor
2
QUANTITATIVE MASS SENSING WITH ENCASED CANTILEVERS

250 nm Gold Particles

mc = 22.88ng

ne =

1
1
= 4 » 0.23
(bi L)4 Ci

Rp = -14.8mHz/fg
Known location of added mass  quantitative mass sensor
QUANTITATIVE MASS SENSING WITH ENCASED CANTILEVERS

250 nm Gold Particles

mc = 22.88ng

ne =

1
1
= 4 » 0.23
(bi L)4 Ci

Rp = -14.8mHz/fg
Cantilever Based Mass Sensing
mavg= 168 ± 12 fg

TEM Based Estimation
mavg= 175 ± 68 fg

Theory Gold Sphere
m=157 fg
SMALLEST DETECTABLE MASS
Current Cantilever Design
l = 125m m
w = 30m m
h = 2.5m m

k 10 N m
0

220kHz

A =100nm

Q 317

dm = 0.1fg / Hz » 60MDa / Hz

Optimized for Mass Sensing
l = 25m m

k = 1700 N m

w = 5m m
h = 5m m

w 0 = 11.0MHz

A =100nm

Q = 4500

dm = 20zg / Hz » 12.5kDa / Hz
Single Very Small Protein
(~100 Carbon-12 Atoms)

~60 Gold Atoms
Each 0.32 zg
SMALLEST DETECTABLE MASS
Current Cantilever Design
l = 125m m
w = 30m m
h = 2.5m m

k 10 N m
0

220kHz

A =100nm

Q 317

dm = 0.1fg / Hz » 60MDa / Hz

Optimized for Mass Sensing
l = 25m m

k = 1700 N m

w = 5m m
h = 5m m

w 0 = 11.0MHz

A =100nm

Q = 4500

dm = 20zg / Hz » 12.5kDa / Hz
Single Very Small Protein
(~100 Carbon-12 Atoms)

~60 Gold Atoms
Each 0.32 zg
INTERFEROMETRIC READOUT

Destructive interference if path
length difference (δ) between two
beams is

Finesse F= δλ/Δλ
Detect intensity of reflected light
(Single photodiode, high BW 50MHz)
k=45N/m
f0=320kHz
F=δλ/Δλ

Finesse ~3

Position Noise ~6fm/√Hz! (@10.6mW,~770nm)
CONCLUSION
Encased cantilevers overcome the problem of
viscous damping in liquids
Compatible with commercial instruments

-Ultra-low force noise in liquids 12 fN/sqrt(Hz)

-Gentle and High-Resolution Imaging
-Water Hydration Layers
-Quantitative Mass Sensor 0.1 fg/sqrt(Hz)

-Interferometric Readout 6 fm/sqrt(Hz)
ACKNOWLEDGEMENTS
Paul D Ashby

Interferometric Readout

Adrian Nievergelt (EPFL, Switzerland)
Technical Support

Ed Wong, Virginia Altoe

Preparation of Lipid Bilayers

David Chmielewski (UC Berkeley)
Modeling of Squeeze Film Damping

Dara Badri (UC Berkeley)
Aram Klaassen (University of Twente, The Netherlands)
Frieder Muegele (University of Twente, The Netherlands)
John Sader (University of Melbourne, Australia)

National Science Foundation
Cyber-enabled Discovery and Innovation #CBET940417

www.foundry.lbl.gov

www.scubaprobe.com

US Department of Energy DOE
#DE-AC02-05CH11231

www.lbl.gov

For more information please contact Dominik Ziegler PhD, dziegler@lbl.gov, +1 510 599 4444

More Related Content

What's hot

Diodo 1 n4004
Diodo 1 n4004Diodo 1 n4004
Diodo 1 n4004
Fidel Chavez Montejano
 
BPV10NF - Datasheet - VISHAY
BPV10NF - Datasheet - VISHAYBPV10NF - Datasheet - VISHAY
BPV10NF - Datasheet - VISHAY
MarioFarias18
 
Aulmighty Capacitor Catalog 09.26
Aulmighty Capacitor Catalog 09.26Aulmighty Capacitor Catalog 09.26
Aulmighty Capacitor Catalog 09.26
frank_lq
 
C&I Cables Tests
C&I Cables TestsC&I Cables Tests
C&I Cables Tests
Pratyusha Khandelwal
 
Vertex Model 13m Cassegrain Antenna
Vertex Model 13m Cassegrain AntennaVertex Model 13m Cassegrain Antenna
Vertex Model 13m Cassegrain Antenna
Syarif Lumintarjo
 
Sebastian keckert status of the hzb quadrupole resonator
Sebastian keckert   status of the hzb quadrupole resonatorSebastian keckert   status of the hzb quadrupole resonator
Sebastian keckert status of the hzb quadrupole resonator
thinfilmsworkshop
 
Manometers and leak detector
Manometers and leak detectorManometers and leak detector
Manometers and leak detector
link2013
 
Bfdx long range red color uv two way radios bf 5111 uv
Bfdx long range red color uv two way radios bf 5111 uvBfdx long range red color uv two way radios bf 5111 uv
Bfdx long range red color uv two way radios bf 5111 uv
Beifeng Telecom Technology Co., Ltd
 
NORSAT_KU-BAND_DRO_4000_LNB
NORSAT_KU-BAND_DRO_4000_LNBNORSAT_KU-BAND_DRO_4000_LNB
NORSAT_KU-BAND_DRO_4000_LNB
Dession485
 
Mtr3000 en
Mtr3000 enMtr3000 en
Quality control (training course)
Quality control (training course)Quality control (training course)
Quality control (training course)
fikireyesus
 

What's hot (11)

Diodo 1 n4004
Diodo 1 n4004Diodo 1 n4004
Diodo 1 n4004
 
BPV10NF - Datasheet - VISHAY
BPV10NF - Datasheet - VISHAYBPV10NF - Datasheet - VISHAY
BPV10NF - Datasheet - VISHAY
 
Aulmighty Capacitor Catalog 09.26
Aulmighty Capacitor Catalog 09.26Aulmighty Capacitor Catalog 09.26
Aulmighty Capacitor Catalog 09.26
 
C&I Cables Tests
C&I Cables TestsC&I Cables Tests
C&I Cables Tests
 
Vertex Model 13m Cassegrain Antenna
Vertex Model 13m Cassegrain AntennaVertex Model 13m Cassegrain Antenna
Vertex Model 13m Cassegrain Antenna
 
Sebastian keckert status of the hzb quadrupole resonator
Sebastian keckert   status of the hzb quadrupole resonatorSebastian keckert   status of the hzb quadrupole resonator
Sebastian keckert status of the hzb quadrupole resonator
 
Manometers and leak detector
Manometers and leak detectorManometers and leak detector
Manometers and leak detector
 
Bfdx long range red color uv two way radios bf 5111 uv
Bfdx long range red color uv two way radios bf 5111 uvBfdx long range red color uv two way radios bf 5111 uv
Bfdx long range red color uv two way radios bf 5111 uv
 
NORSAT_KU-BAND_DRO_4000_LNB
NORSAT_KU-BAND_DRO_4000_LNBNORSAT_KU-BAND_DRO_4000_LNB
NORSAT_KU-BAND_DRO_4000_LNB
 
Mtr3000 en
Mtr3000 enMtr3000 en
Mtr3000 en
 
Quality control (training course)
Quality control (training course)Quality control (training course)
Quality control (training course)
 

Similar to Encased Cantilevers for Low-Noise Mass and Force Sensing in Liquids

2012 fallmrs encasedlevers_dominikziegler_summary
2012 fallmrs encasedlevers_dominikziegler_summary2012 fallmrs encasedlevers_dominikziegler_summary
2012 fallmrs encasedlevers_dominikziegler_summary
Dominik Ziegler
 
HDR Vincent Agache Defense
HDR Vincent Agache DefenseHDR Vincent Agache Defense
HDR Vincent Agache Defense
vince3859
 
Poster
PosterPoster
Poster
yantmlch
 
Masters Thesis Presentation Octavio Lora 2016
Masters Thesis Presentation Octavio Lora 2016Masters Thesis Presentation Octavio Lora 2016
Masters Thesis Presentation Octavio Lora 2016
Octavio Lora Aranda
 
APD.ppt
APD.pptAPD.ppt
APD.ppt
ponni2
 
PhD Seminar David Dahan 2005
PhD Seminar David Dahan 2005PhD Seminar David Dahan 2005
PhD Seminar David Dahan 2005
David Dahan
 
FR1.T03.5 IGARSS_2011_Albers_22JUL2011.ppt
FR1.T03.5 IGARSS_2011_Albers_22JUL2011.pptFR1.T03.5 IGARSS_2011_Albers_22JUL2011.ppt
FR1.T03.5 IGARSS_2011_Albers_22JUL2011.ppt
grssieee
 
FUNDAMENTAL ACOUSTICS AND WIND TURBINE NOISE ISSUES
FUNDAMENTAL ACOUSTICS AND WIND TURBINE NOISE ISSUESFUNDAMENTAL ACOUSTICS AND WIND TURBINE NOISE ISSUES
FUNDAMENTAL ACOUSTICS AND WIND TURBINE NOISE ISSUES
riseagrant
 
FUNDAMENTAL ACOUSTICS AND WIND TURBINE NOISE ISSUES
FUNDAMENTAL ACOUSTICS AND WIND TURBINE NOISE ISSUESFUNDAMENTAL ACOUSTICS AND WIND TURBINE NOISE ISSUES
FUNDAMENTAL ACOUSTICS AND WIND TURBINE NOISE ISSUES
riseagrant
 
Channel Models for Massive MIMO
Channel Models for Massive MIMOChannel Models for Massive MIMO
Channel Models for Massive MIMO
CPqD
 
Hsb 2011 diffuse
Hsb 2011 diffuseHsb 2011 diffuse
EXFO OSA20 (Optical Spectrum Analyzer)
EXFO OSA20 (Optical Spectrum Analyzer)EXFO OSA20 (Optical Spectrum Analyzer)
EXFO OSA20 (Optical Spectrum Analyzer)
INVAS Technologies Pvt. Ltd.
 
Noiseken ins220 Impulse Noise Simulator denkei
Noiseken ins220 Impulse Noise Simulator denkeiNoiseken ins220 Impulse Noise Simulator denkei
Noiseken ins220 Impulse Noise Simulator denkei
NIHON DENKEI SINGAPORE
 
Noiseken ins220 Impulse Noise Simulator denkei
Noiseken ins220 Impulse Noise Simulator denkeiNoiseken ins220 Impulse Noise Simulator denkei
Noiseken ins220 Impulse Noise Simulator denkei
NIHON DENKEI SINGAPORE
 
A universal matter-wave interferometer with optical gratings in the time domain
A universal matter-wave interferometer with optical gratings in the time domainA universal matter-wave interferometer with optical gratings in the time domain
A universal matter-wave interferometer with optical gratings in the time domain
PhysikUndMusik
 
Xray interferometry
Xray interferometryXray interferometry
Xray interferometry
Clifford Stone
 
Sub Bottom Profiler QGI.pdf
Sub Bottom Profiler QGI.pdfSub Bottom Profiler QGI.pdf
Sub Bottom Profiler QGI.pdf
Muhammad Bani Al Rasyid
 
OTDR(OPTICAL TIME DOMAIN REFLECTOMETER)
OTDR(OPTICAL TIME DOMAIN REFLECTOMETER)OTDR(OPTICAL TIME DOMAIN REFLECTOMETER)
OTDR(OPTICAL TIME DOMAIN REFLECTOMETER)
Shail Mishra
 
ultrasonic
ultrasonicultrasonic
ultrasonic
stanley christy
 
Microstrip antennas
Microstrip antennasMicrostrip antennas
Microstrip antennas
Suresh Khaleri
 

Similar to Encased Cantilevers for Low-Noise Mass and Force Sensing in Liquids (20)

2012 fallmrs encasedlevers_dominikziegler_summary
2012 fallmrs encasedlevers_dominikziegler_summary2012 fallmrs encasedlevers_dominikziegler_summary
2012 fallmrs encasedlevers_dominikziegler_summary
 
HDR Vincent Agache Defense
HDR Vincent Agache DefenseHDR Vincent Agache Defense
HDR Vincent Agache Defense
 
Poster
PosterPoster
Poster
 
Masters Thesis Presentation Octavio Lora 2016
Masters Thesis Presentation Octavio Lora 2016Masters Thesis Presentation Octavio Lora 2016
Masters Thesis Presentation Octavio Lora 2016
 
APD.ppt
APD.pptAPD.ppt
APD.ppt
 
PhD Seminar David Dahan 2005
PhD Seminar David Dahan 2005PhD Seminar David Dahan 2005
PhD Seminar David Dahan 2005
 
FR1.T03.5 IGARSS_2011_Albers_22JUL2011.ppt
FR1.T03.5 IGARSS_2011_Albers_22JUL2011.pptFR1.T03.5 IGARSS_2011_Albers_22JUL2011.ppt
FR1.T03.5 IGARSS_2011_Albers_22JUL2011.ppt
 
FUNDAMENTAL ACOUSTICS AND WIND TURBINE NOISE ISSUES
FUNDAMENTAL ACOUSTICS AND WIND TURBINE NOISE ISSUESFUNDAMENTAL ACOUSTICS AND WIND TURBINE NOISE ISSUES
FUNDAMENTAL ACOUSTICS AND WIND TURBINE NOISE ISSUES
 
FUNDAMENTAL ACOUSTICS AND WIND TURBINE NOISE ISSUES
FUNDAMENTAL ACOUSTICS AND WIND TURBINE NOISE ISSUESFUNDAMENTAL ACOUSTICS AND WIND TURBINE NOISE ISSUES
FUNDAMENTAL ACOUSTICS AND WIND TURBINE NOISE ISSUES
 
Channel Models for Massive MIMO
Channel Models for Massive MIMOChannel Models for Massive MIMO
Channel Models for Massive MIMO
 
Hsb 2011 diffuse
Hsb 2011 diffuseHsb 2011 diffuse
Hsb 2011 diffuse
 
EXFO OSA20 (Optical Spectrum Analyzer)
EXFO OSA20 (Optical Spectrum Analyzer)EXFO OSA20 (Optical Spectrum Analyzer)
EXFO OSA20 (Optical Spectrum Analyzer)
 
Noiseken ins220 Impulse Noise Simulator denkei
Noiseken ins220 Impulse Noise Simulator denkeiNoiseken ins220 Impulse Noise Simulator denkei
Noiseken ins220 Impulse Noise Simulator denkei
 
Noiseken ins220 Impulse Noise Simulator denkei
Noiseken ins220 Impulse Noise Simulator denkeiNoiseken ins220 Impulse Noise Simulator denkei
Noiseken ins220 Impulse Noise Simulator denkei
 
A universal matter-wave interferometer with optical gratings in the time domain
A universal matter-wave interferometer with optical gratings in the time domainA universal matter-wave interferometer with optical gratings in the time domain
A universal matter-wave interferometer with optical gratings in the time domain
 
Xray interferometry
Xray interferometryXray interferometry
Xray interferometry
 
Sub Bottom Profiler QGI.pdf
Sub Bottom Profiler QGI.pdfSub Bottom Profiler QGI.pdf
Sub Bottom Profiler QGI.pdf
 
OTDR(OPTICAL TIME DOMAIN REFLECTOMETER)
OTDR(OPTICAL TIME DOMAIN REFLECTOMETER)OTDR(OPTICAL TIME DOMAIN REFLECTOMETER)
OTDR(OPTICAL TIME DOMAIN REFLECTOMETER)
 
ultrasonic
ultrasonicultrasonic
ultrasonic
 
Microstrip antennas
Microstrip antennasMicrostrip antennas
Microstrip antennas
 

Recently uploaded

Taking AI to the Next Level in Manufacturing.pdf
Taking AI to the Next Level in Manufacturing.pdfTaking AI to the Next Level in Manufacturing.pdf
Taking AI to the Next Level in Manufacturing.pdf
ssuserfac0301
 
Main news related to the CCS TSI 2023 (2023/1695)
Main news related to the CCS TSI 2023 (2023/1695)Main news related to the CCS TSI 2023 (2023/1695)
Main news related to the CCS TSI 2023 (2023/1695)
Jakub Marek
 
5th LF Energy Power Grid Model Meet-up Slides
5th LF Energy Power Grid Model Meet-up Slides5th LF Energy Power Grid Model Meet-up Slides
5th LF Energy Power Grid Model Meet-up Slides
DanBrown980551
 
“Temporal Event Neural Networks: A More Efficient Alternative to the Transfor...
“Temporal Event Neural Networks: A More Efficient Alternative to the Transfor...“Temporal Event Neural Networks: A More Efficient Alternative to the Transfor...
“Temporal Event Neural Networks: A More Efficient Alternative to the Transfor...
Edge AI and Vision Alliance
 
Generating privacy-protected synthetic data using Secludy and Milvus
Generating privacy-protected synthetic data using Secludy and MilvusGenerating privacy-protected synthetic data using Secludy and Milvus
Generating privacy-protected synthetic data using Secludy and Milvus
Zilliz
 
Overcoming the PLG Trap: Lessons from Canva's Head of Sales & Head of EMEA Da...
Overcoming the PLG Trap: Lessons from Canva's Head of Sales & Head of EMEA Da...Overcoming the PLG Trap: Lessons from Canva's Head of Sales & Head of EMEA Da...
Overcoming the PLG Trap: Lessons from Canva's Head of Sales & Head of EMEA Da...
saastr
 
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
Your One-Stop Shop for Python Success: Top 10 US Python Development ProvidersYour One-Stop Shop for Python Success: Top 10 US Python Development Providers
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
akankshawande
 
Programming Foundation Models with DSPy - Meetup Slides
Programming Foundation Models with DSPy - Meetup SlidesProgramming Foundation Models with DSPy - Meetup Slides
Programming Foundation Models with DSPy - Meetup Slides
Zilliz
 
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Jeffrey Haguewood
 
HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAU
HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAUHCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAU
HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAU
panagenda
 
Introduction of Cybersecurity with OSS at Code Europe 2024
Introduction of Cybersecurity with OSS  at Code Europe 2024Introduction of Cybersecurity with OSS  at Code Europe 2024
Introduction of Cybersecurity with OSS at Code Europe 2024
Hiroshi SHIBATA
 
Digital Marketing Trends in 2024 | Guide for Staying Ahead
Digital Marketing Trends in 2024 | Guide for Staying AheadDigital Marketing Trends in 2024 | Guide for Staying Ahead
Digital Marketing Trends in 2024 | Guide for Staying Ahead
Wask
 
Monitoring and Managing Anomaly Detection on OpenShift.pdf
Monitoring and Managing Anomaly Detection on OpenShift.pdfMonitoring and Managing Anomaly Detection on OpenShift.pdf
Monitoring and Managing Anomaly Detection on OpenShift.pdf
Tosin Akinosho
 
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with SlackLet's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
shyamraj55
 
Public CyberSecurity Awareness Presentation 2024.pptx
Public CyberSecurity Awareness Presentation 2024.pptxPublic CyberSecurity Awareness Presentation 2024.pptx
Public CyberSecurity Awareness Presentation 2024.pptx
marufrahmanstratejm
 
dbms calicut university B. sc Cs 4th sem.pdf
dbms  calicut university B. sc Cs 4th sem.pdfdbms  calicut university B. sc Cs 4th sem.pdf
dbms calicut university B. sc Cs 4th sem.pdf
Shinana2
 
A Comprehensive Guide to DeFi Development Services in 2024
A Comprehensive Guide to DeFi Development Services in 2024A Comprehensive Guide to DeFi Development Services in 2024
A Comprehensive Guide to DeFi Development Services in 2024
Intelisync
 
TrustArc Webinar - 2024 Global Privacy Survey
TrustArc Webinar - 2024 Global Privacy SurveyTrustArc Webinar - 2024 Global Privacy Survey
TrustArc Webinar - 2024 Global Privacy Survey
TrustArc
 
JavaLand 2024: Application Development Green Masterplan
JavaLand 2024: Application Development Green MasterplanJavaLand 2024: Application Development Green Masterplan
JavaLand 2024: Application Development Green Masterplan
Miro Wengner
 
AWS Cloud Cost Optimization Presentation.pptx
AWS Cloud Cost Optimization Presentation.pptxAWS Cloud Cost Optimization Presentation.pptx
AWS Cloud Cost Optimization Presentation.pptx
HarisZaheer8
 

Recently uploaded (20)

Taking AI to the Next Level in Manufacturing.pdf
Taking AI to the Next Level in Manufacturing.pdfTaking AI to the Next Level in Manufacturing.pdf
Taking AI to the Next Level in Manufacturing.pdf
 
Main news related to the CCS TSI 2023 (2023/1695)
Main news related to the CCS TSI 2023 (2023/1695)Main news related to the CCS TSI 2023 (2023/1695)
Main news related to the CCS TSI 2023 (2023/1695)
 
5th LF Energy Power Grid Model Meet-up Slides
5th LF Energy Power Grid Model Meet-up Slides5th LF Energy Power Grid Model Meet-up Slides
5th LF Energy Power Grid Model Meet-up Slides
 
“Temporal Event Neural Networks: A More Efficient Alternative to the Transfor...
“Temporal Event Neural Networks: A More Efficient Alternative to the Transfor...“Temporal Event Neural Networks: A More Efficient Alternative to the Transfor...
“Temporal Event Neural Networks: A More Efficient Alternative to the Transfor...
 
Generating privacy-protected synthetic data using Secludy and Milvus
Generating privacy-protected synthetic data using Secludy and MilvusGenerating privacy-protected synthetic data using Secludy and Milvus
Generating privacy-protected synthetic data using Secludy and Milvus
 
Overcoming the PLG Trap: Lessons from Canva's Head of Sales & Head of EMEA Da...
Overcoming the PLG Trap: Lessons from Canva's Head of Sales & Head of EMEA Da...Overcoming the PLG Trap: Lessons from Canva's Head of Sales & Head of EMEA Da...
Overcoming the PLG Trap: Lessons from Canva's Head of Sales & Head of EMEA Da...
 
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
Your One-Stop Shop for Python Success: Top 10 US Python Development ProvidersYour One-Stop Shop for Python Success: Top 10 US Python Development Providers
Your One-Stop Shop for Python Success: Top 10 US Python Development Providers
 
Programming Foundation Models with DSPy - Meetup Slides
Programming Foundation Models with DSPy - Meetup SlidesProgramming Foundation Models with DSPy - Meetup Slides
Programming Foundation Models with DSPy - Meetup Slides
 
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
 
HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAU
HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAUHCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAU
HCL Notes und Domino Lizenzkostenreduzierung in der Welt von DLAU
 
Introduction of Cybersecurity with OSS at Code Europe 2024
Introduction of Cybersecurity with OSS  at Code Europe 2024Introduction of Cybersecurity with OSS  at Code Europe 2024
Introduction of Cybersecurity with OSS at Code Europe 2024
 
Digital Marketing Trends in 2024 | Guide for Staying Ahead
Digital Marketing Trends in 2024 | Guide for Staying AheadDigital Marketing Trends in 2024 | Guide for Staying Ahead
Digital Marketing Trends in 2024 | Guide for Staying Ahead
 
Monitoring and Managing Anomaly Detection on OpenShift.pdf
Monitoring and Managing Anomaly Detection on OpenShift.pdfMonitoring and Managing Anomaly Detection on OpenShift.pdf
Monitoring and Managing Anomaly Detection on OpenShift.pdf
 
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with SlackLet's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
 
Public CyberSecurity Awareness Presentation 2024.pptx
Public CyberSecurity Awareness Presentation 2024.pptxPublic CyberSecurity Awareness Presentation 2024.pptx
Public CyberSecurity Awareness Presentation 2024.pptx
 
dbms calicut university B. sc Cs 4th sem.pdf
dbms  calicut university B. sc Cs 4th sem.pdfdbms  calicut university B. sc Cs 4th sem.pdf
dbms calicut university B. sc Cs 4th sem.pdf
 
A Comprehensive Guide to DeFi Development Services in 2024
A Comprehensive Guide to DeFi Development Services in 2024A Comprehensive Guide to DeFi Development Services in 2024
A Comprehensive Guide to DeFi Development Services in 2024
 
TrustArc Webinar - 2024 Global Privacy Survey
TrustArc Webinar - 2024 Global Privacy SurveyTrustArc Webinar - 2024 Global Privacy Survey
TrustArc Webinar - 2024 Global Privacy Survey
 
JavaLand 2024: Application Development Green Masterplan
JavaLand 2024: Application Development Green MasterplanJavaLand 2024: Application Development Green Masterplan
JavaLand 2024: Application Development Green Masterplan
 
AWS Cloud Cost Optimization Presentation.pptx
AWS Cloud Cost Optimization Presentation.pptxAWS Cloud Cost Optimization Presentation.pptx
AWS Cloud Cost Optimization Presentation.pptx
 

Encased Cantilevers for Low-Noise Mass and Force Sensing in Liquids

  • 1. ENCASED CANTILEVERS FOR LOW-NOISE FORCE AND MASS SENSING IN LIQUIDS Dominik Ziegler PhD Material Science Division, Molecular Foundry, Lawrence Berkeley National Laboratory
  • 2. High Resolution Imaging of Mica Encased Cantilevers Fabrication Quantitative Mass Sensor Gentle Imaging of Lipid Bilayers Interferometric Deflection Detection Dominik Ziegler PhD Material Science Division, Molecular Foundry, Lawrence Berkeley National Laboratory
  • 3. VISCOUS DAMPING LIMITS RESOLUTION Fn  4k BTk 0Qi 4k BTb Force Noise  Smallest Measurable Force ω0≈150 kHz Q≈200 ω0≈50 kHz Q≈4 Fn air≈15fN/√Hz Fn water ≈200fN/√Hz 100 m long, 30 m wide  High forces deform soft samples and limits resolution
  • 4. LET`S GO SCUBA DIVING!
  • 5. FABRICATION OF ENCASED CANTILEVERS -Length of levers can be tuned -Tip’s sharpness is maintained
  • 6. FABRICATION OF ENCASED CANTILEVERS -Length of levers can be tuned -Tip’s sharpness is maintained
  • 7. ENCASED CANTILEVER IN AIR/WATER air water fair=310.280kHz fwater=309.71kHz Qair=210 Qwater=133 Single clean resonance peak. High Q and high frequency, performance as in air (Fn=12 fN/sqrt(Hz)) -Only small frequency shift (1%)  No Added Mass -Viscous damping of tip (few um) and double sided squeeze film damping
  • 8. GENTLE IMAGING OF LIPID BILAYERS height of supported DPPC bilayers on mica softest possible imaging amplitude modulation “Hydra Levers” by AppNano Encased silicon lever (Mikromasch NSC36) L = 200 µm, k=0.084N/m f=17 kHz l =110 µm, k = 0.95N/m, f=85kHz Thicker measured height  Less deformation by tip
  • 9. HIGH-RESOLUTION IMAGING OF MICA IN LIQUID 0.5 nm MICA lattice using amplitude modulation 300mM KCl, 10mM K2HPO4, 5 lines/s Unmodified commercial instrument Multimode, Bruker
  • 10. OSCILLATORY HYDRATION FORCE Amplitude[mV] Amplitude [Å] (WATER STRUCTURE ON MICA) 20 15 10 5 0 0 1 0 1 Phase [Deg] Phase[Deg] 60 3 2 [nm] 2 3 0.5 nm 40 20 0 -20 [nm] ~4Å Ultra-small amplitude displacement of the last few water layers single curve no averaging Kobayashi, J. CHEM. PHYS. 138, 184704 (2013)
  • 11. QUANTITATIVE MASS SENSING WITH ENCASED CANTILEVERS Indirect measurement of mass over surface stress (DC measurement) 0 Known location of added mass  quantitative mass sensor 2
  • 12. QUANTITATIVE MASS SENSING WITH ENCASED CANTILEVERS 250 nm Gold Particles mc = 22.88ng ne = 1 1 = 4 » 0.23 (bi L)4 Ci Rp = -14.8mHz/fg Known location of added mass  quantitative mass sensor
  • 13. QUANTITATIVE MASS SENSING WITH ENCASED CANTILEVERS 250 nm Gold Particles mc = 22.88ng ne = 1 1 = 4 » 0.23 (bi L)4 Ci Rp = -14.8mHz/fg Cantilever Based Mass Sensing mavg= 168 ± 12 fg TEM Based Estimation mavg= 175 ± 68 fg Theory Gold Sphere m=157 fg
  • 14. SMALLEST DETECTABLE MASS Current Cantilever Design l = 125m m w = 30m m h = 2.5m m k 10 N m 0 220kHz A =100nm Q 317 dm = 0.1fg / Hz » 60MDa / Hz Optimized for Mass Sensing l = 25m m k = 1700 N m w = 5m m h = 5m m w 0 = 11.0MHz A =100nm Q = 4500 dm = 20zg / Hz » 12.5kDa / Hz Single Very Small Protein (~100 Carbon-12 Atoms) ~60 Gold Atoms Each 0.32 zg
  • 15. SMALLEST DETECTABLE MASS Current Cantilever Design l = 125m m w = 30m m h = 2.5m m k 10 N m 0 220kHz A =100nm Q 317 dm = 0.1fg / Hz » 60MDa / Hz Optimized for Mass Sensing l = 25m m k = 1700 N m w = 5m m h = 5m m w 0 = 11.0MHz A =100nm Q = 4500 dm = 20zg / Hz » 12.5kDa / Hz Single Very Small Protein (~100 Carbon-12 Atoms) ~60 Gold Atoms Each 0.32 zg
  • 16. INTERFEROMETRIC READOUT Destructive interference if path length difference (δ) between two beams is Finesse F= δλ/Δλ Detect intensity of reflected light (Single photodiode, high BW 50MHz) k=45N/m f0=320kHz F=δλ/Δλ Finesse ~3 Position Noise ~6fm/√Hz! (@10.6mW,~770nm)
  • 17. CONCLUSION Encased cantilevers overcome the problem of viscous damping in liquids Compatible with commercial instruments -Ultra-low force noise in liquids 12 fN/sqrt(Hz) -Gentle and High-Resolution Imaging -Water Hydration Layers -Quantitative Mass Sensor 0.1 fg/sqrt(Hz) -Interferometric Readout 6 fm/sqrt(Hz)
  • 18. ACKNOWLEDGEMENTS Paul D Ashby Interferometric Readout Adrian Nievergelt (EPFL, Switzerland) Technical Support Ed Wong, Virginia Altoe Preparation of Lipid Bilayers David Chmielewski (UC Berkeley) Modeling of Squeeze Film Damping Dara Badri (UC Berkeley) Aram Klaassen (University of Twente, The Netherlands) Frieder Muegele (University of Twente, The Netherlands) John Sader (University of Melbourne, Australia) National Science Foundation Cyber-enabled Discovery and Innovation #CBET940417 www.foundry.lbl.gov www.scubaprobe.com US Department of Energy DOE #DE-AC02-05CH11231 www.lbl.gov For more information please contact Dominik Ziegler PhD, dziegler@lbl.gov, +1 510 599 4444

Editor's Notes

  1. Tip sticks out by only several 3-5 um, Maskless Post-processing of Regular Silicon CantileversAdjust Final length by Sacrificial Layer Release
  2. Tip sticks out by only several 3-5 um, Maskless Post-processing of Regular Silicon CantileversMight look simple, but important that depositons are uniform and free of stress.
  3. Sometimes positive frequency shift, repulsive, I,e. cantilever hydrophobic.
  4. High-Angle Annular Dark-Field (HAADF) Microscopy
  5. High-Angle Annular Dark-Field (HAADF) Microscopy
  6. STEM HAADF, Icosahedral dodecahedron.
  7. If delta is Odd multiple of lambda/2----- Meeting Notes (10/3/12 04:02) -----Add OBD