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Eddy's Hot plate analgesiometer kamal.pptx

The document describes the operation of Eddy's hot plate analgesiometer to assess the analgesic effects of the opioid tramadol in rodents. It outlines the procedure for conducting the experiment, including setting temperature parameters, observing animal reactions, and recording data. Additionally, it provides guidance on animal grouping, dosage administration, and the requirement for maintaining temperature consistency for accurate results.

Education◦
Eddy’s Hot Plate analgesiometer apparatus By: Dr. Kamal Akhtar (JR1) Junior resident Dept. of Pharmacology & Therapeutics KGMU, Lucknow
Aim • To demonstrate the analgesic effect of Opiod analgesics in rodents using Eddy’s hot plate analgesiometer.
Principle • Rodents will show aversion effect to thermal stimulus by paw licking & jumping after placing onto the hot surface • Source of pain: Contact heat
• Endpoint: Paw licking and jumping • Cut off time : Mouse - 15 – 20 sec Rat - 20 – 30 sec
Materials • Weighing balance • Eddy’s hot plate analgesiometer • Rats • Drug: Tramadol • Needle: 24 gauge • Gloves
Eddy s Hot Plate ′ Perspex glass Cover Lid Lid sensor Aluminium Plate
• Front view :- Indicator Light Keys
• Indicator lights: 1) TEMPT: Indication of heating is on Light blinking - heating process to achieve the temperature is going on Light stable - set temperature is achieved 2) LID: Indication of Lid of the plate is open
Keys - • OK: To enter the set parameters • :To move forward or to increase the value • <: :To move backward or to decrease the value • BACK : To go back to previous function or escape the current function
• Lid sensor-
Instrument has four mode - • Start New Experiment • Continue Previous Experiment • View logs • PC connectivity
1. Start New Experiment: To start with new exp. - Press OK once Start - New Exp. appears on the screen
• Set cut off time:-
• Set temperature :
• Heating aluminium plate :
• The Screen will show heating, please wait to indicate heating is going on • Exp. indicating the experiment number • S. No. indicating the Sequence Number • S. Temp indicating Set temperature value • P. Temp indicating present plate temperature value
• On opening the lid of the chamber, the green light marked as LID on the control panel will glow indicating that lid is open • Indication to remind the user to close the lid to avoid temperature fluctuations
• Temp - light will keep blinking till the set temperature is achieved • Once the plate temperature is equal to set temperature value, the display will show place animal
• Place the animal on the plate surface and press foot switch • Keep foot switch pressed till the reaction time is noted • Observe the reaction time by noting the jumping response or the licking of paws • As soon as you observe the jumping response, release the pedal switch so as to stop the timer and note down the reaction time
• Once the foot switch is released after watching reaction time, reading for reaction time will be saved in the log
• Test compounds are administered i.p. and latency period is recorded after 20, 60 and 90 min
2. Continue Previous Experiment: To work in previous experiment
3. View Logs: To view the earlier experiments data
4. PC Connectivity: To connect with computer for data transfer
Tramadol in hot plate analgesiometer • Dose – 20 mg/kg • Route - intraperitoneal
Procedure : during testing • Weigh the animals and mark properly to distinguish from one another • Divide animals into two groups (n = 6 in each group) Group 1: Control group Rats are given saline at the equivalent dose of drug
Group 2: Treatment group Rats are given Tramadol (20 mg/kg)
Weigh the animal and mark properly to distinguish from one another Put the animals on hot plate analgesiometer Observe the animals for response (Paw licking or jumping) Make the observation table
0bservation table (Control) S.No Animal Weight (gms) Basal Reaction Time (sec) Reaction time (sec) 20 mins 60 mins 90 mins 1. 165 4.8 4.4 4.1 4.8 2. 182 5.4 5.2 5.2 4.4 3. 167 5.2 5.0 5.4 4.8 4. 175 5.6 4.8 4.6 4.4 5. 185 3.8 4.0 4.4 4.2 6. 174 4.6 4.5 4.8 4.6
0bservation table (Test) S.No Animal Weight (gms) Basal Reaction Time (sec) Reaction time after treatment (sec) 20 mins 60 mins 90 mins 1. 172 3.4 6.3 4.0 3.6 2. 190 4.2 7.2 4.5 4.5 3. 165 5.1 7.6 5.6 5.2 4. 176 4.4 5.2 4.7 4.6 5. 190 5.4 6.1 5.8 5.6 6. 170 4.5 5.5 5.0 4.7
Data analysis and results • The following response are observed to note the reaction time: - Paw licking - Jumping response
Precaution: • Always note that the lid of plate is enclosed to get accurate and fast temperature • If plate temperature fluctuates, wait till the plate temperature is achieved to set value • Clean the paw and hot plate for uniform temperature distribution
References- • Medhi B, Prakash A. Practical Manual of Experimental and Clinical Pharmacology. 2nd ed. New Delhi: Jaypee Brothers Medical Publishers; 2017. 201-203 p.
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