SlideShare a Scribd company logo
1 of 10
Download to read offline
DIY culture media for kitchen experiments

1
Background 1: food safe DIY culture
In past experiments, “Green Dakara” sports drink could be blended with
DMEM up to 60% without losing cell culture efficacy.
https://www.slideshare.net/2co/shojinmeat-project-cellular-agriculture-initiative-nov2018
However, all ingredients must be replaced with commonly available
materials to enable kitchens DIY cell culture experiments.
※Research grade reagents = Not approved as food and not commonly available.
Food or food additives that can be ingested (within limits) are commonly available.
Basal media (DMEM, L15) was prepared entirely from commonly
available materials and a DIY cell culture was demonstrated.
Background 2: composition of DMEM and L15
The biggest difference between DMEM and L15 is in the minerals.
Other ingredients were assumed common.
Precisely, DMEM and L15 have different amino acid compositions and DMEM uses glucose while L15 uses galactose
DMEM⇒https://www.sigmaaldrich.com/life-science/cell-culture/learning-center/media-formulations/dme.html
L15⇒ https://www.sigmaaldrich.com/life-science/cell-culture/learning-center/media-formulations/l-15.html
DMEM
L15
20 amino
acids
20 amino
acids
Water-
soluble
vitamin B’s
Water-
soluble
vitamin B’s
Glucose
(dextrose)
Glucose
(dextrose)
CaCl2, MgSO4, KCl, NaCl,
NaHCO3, NaH2PO4, Iron
nitrate
CaCl2, MgCl2, MgSO4, KCl,
NaCl, KH2PO4, Na2HPO4
Ingredient 1 (Sugar, animo acids, vitamin B)
0.32g per tablet
0.22g amino acid
(+sugar & fiber)
1 tablet (0.2g,
incl. shellac
shell) for 2L.
Glucose for
baking -
Use 4.5g/L
https://item.rakuten.co.jp/fancl-shop/5272/?i
asid=07rpp_10095___e4-jpeuuk00-aev-615
07def-77bf-41df-8809-18631a826757
https://item.rakuten.co.jp/
kenkocom/x252760h/
https://item.rakuten.co.jp/a
uc-garlic/budoutou-3000/
Ingredient 2 (Minerals for DMEM)
https://item.rakuten.co.jp/m
amoru/4571474270191/
https://item.rakuten.co.jp/m
atsubayakuhin/10000000/
CaCl2 (calcium
chloride)
NaCl(salt)
https://item.rakuten.co.jp/
jyugo/t-4987306019126/
Half Salt
NaCl/KCl mixed
at 1:1
MgSO4 
(Magnesium sulfate)
Mixing at 1:1 molar
quantity makes NaH2PO4
※Iron nitrate not included
・Only miniscule amount is needed.
・Iron nitrate is not commonly available - not a food additive.
・Replaceable with iron (III) chloride?
baking
soda
https://www.amazon.co.jp/%E
3%82%AA%E3%83%AB%E3
%83%88%E3%83%AA%E3%
83%B3%E9%85%B8-%E3%8
3%AA%E3%83%B3%E9%85
%B8%EF%BC%98%EF%BC
%95%EF%BC%85%E3%80%
8C%E9%A3%9F%E5%93%81
%E6%B7%BB%E5%8A%A0%
E7%89%A9%E3%80%8D%E
F%BC%95%EF%BC%90%EF
%BC%90%EF%BD%87/dp/B0
062HL4PU
phosphoric
acid
Ingredient 2 (Minerals for L15)
https://item.rakuten.co.jp/
mamoru/4571474270177/
MgCl2 
(magnesium
chloride)
https://item.rakuten.co.jp/
kurimoto/100001011/
NaCl(salt)
https://item.rakuten.co.jp/
jyugo/t-4987306019126/
Half salt
1:1 NaCl/KCl
mixture https://www.amazon.co.jp/%E
3%82%AA%E3%83%AB%E3
%83%88%E3%83%AA%E3%
83%B3%E9%85%B8-%E3%8
3%AA%E3%83%B3%E9%85
%B8%EF%BC%98%EF%BC
%95%EF%BC%85%E3%80%
8C%E9%A3%9F%E5%93%81
%E6%B7%BB%E5%8A%A0%
E7%89%A9%E3%80%8D%E
F%BC%95%EF%BC%90%EF
%BC%90%EF%BD%87/dp/B0
062HL4PU
Mixing at 1:1 molar
quantity makes
NaH2PO4
https://item.rakuten.co.jp/m
amoru/4571474270191/
MgSO4 
(Magnesium sulfate)
potassium
carbonate
Mixing at 1:1 molar
quantity makes
KH2PO4
baking
soda
phosphoric
acid
1X Solution 20X Solution
Synthesis method
DMEM:
Table salt 0.58g
baking soda 0.37g
Half salt 0.13g
L15:
Table NaCl 0.95g
Half salt 0.10g
125ml water
1 amino acid
tablet
+
99ml water
1 vitamin B pill
General strategy for preparation
・Prepare 1X concentrated solution for salts, glucose, and amino acid, for which large quantity is needed.
・Prepare 20X solution for vitamin B’s and other minerals, for which only trace amount is needed.
・Prepare phosphates from phosphoric acid and mineral salts.
DMEM:
CaCl2 0.50g
MgSO4 0.25g
baking soda
0.19g
Phosphoric acid
diluted 10X 1.4ml
L15:
CaCl2 0.28g
MgSO4 0.20g
MgCl2 0.19g
K2CO3 0.06g
Phosphoric acid
diluted 10X 1.4ml
Mix 1X solution and 20x solution by 19:1, add glucose at 4.5g/L and bon apetit
Scenes from the experiment
・Filtration is needed, as the tablet excipients need to be removed.
・Wait until the suspension settles to avoid clogging the filter paper.
Holding position
with a peeler
Filtered 1X
solution
Cell culture demonstration
Primary culture of chicken foetal
heart cells by DIY-DMEM:
Successful cell adhesion and
proliferation
(culturing experiment by @thesow41 on twitter)
https://www.nicovideo.jp/watch/sm33104702
※DIY-L15 to be tested
Participate in improving this manual!
https://docs.google.com/presentation/d/1cNedlmZcW7JOKB-gM
itL3aIix1YXd5gi0hhlYz1mVqQ/edit#slide=id.g5683e89615_0_0
Source file openly
accessible and editable

More Related Content

More from 2co

細胞農業の普及シナリオ
細胞農業の普及シナリオ細胞農業の普及シナリオ
細胞農業の普及シナリオ2co
 
New Harvest 2020 slides from Shojinmeat Project - Will there be a monopoly co...
New Harvest 2020 slides from Shojinmeat Project - Will there be a monopoly co...New Harvest 2020 slides from Shojinmeat Project - Will there be a monopoly co...
New Harvest 2020 slides from Shojinmeat Project - Will there be a monopoly co...2co
 
Cellular agriculture in space
Cellular agriculture in spaceCellular agriculture in space
Cellular agriculture in space2co
 
宇宙細胞農業
宇宙細胞農業宇宙細胞農業
宇宙細胞農業2co
 
EmTechAsia 2020 (postponed) Cell-based meat
EmTechAsia 2020 (postponed) Cell-based meatEmTechAsia 2020 (postponed) Cell-based meat
EmTechAsia 2020 (postponed) Cell-based meat2co
 
DIY細胞培養マニュアル
DIY細胞培養マニュアルDIY細胞培養マニュアル
DIY細胞培養マニュアル2co
 
純肉:細胞培養による食料生産(2020版)
純肉:細胞培養による食料生産(2020版)純肉:細胞培養による食料生産(2020版)
純肉:細胞培養による食料生産(2020版)2co
 
DIY cell culture manual (& the roadmap to DIY cell-based meat)
DIY cell culture manual (& the roadmap to DIY cell-based meat)DIY cell culture manual (& the roadmap to DIY cell-based meat)
DIY cell culture manual (& the roadmap to DIY cell-based meat)2co
 
Shojinmeat Project - Open source cellular agriculture initiative
Shojinmeat Project - Open source cellular agriculture initiativeShojinmeat Project - Open source cellular agriculture initiative
Shojinmeat Project - Open source cellular agriculture initiative2co
 
Roadmap to the Cellular Agriculture Era
Roadmap to the Cellular Agriculture EraRoadmap to the Cellular Agriculture Era
Roadmap to the Cellular Agriculture Era2co
 
The cellular agriculture farmscape
The cellular agriculture farmscapeThe cellular agriculture farmscape
The cellular agriculture farmscape2co
 
細胞農業の普及シナリオ
細胞農業の普及シナリオ細胞農業の普及シナリオ
細胞農業の普及シナリオ2co
 
植物肉・培養肉・AT Kearney社の分析レポート訳&まとめ
植物肉・培養肉・AT Kearney社の分析レポート訳&まとめ植物肉・培養肉・AT Kearney社の分析レポート訳&まとめ
植物肉・培養肉・AT Kearney社の分析レポート訳&まとめ2co
 
细胞农业的未来
细胞农业的未来细胞农业的未来
细胞农业的未来2co
 
創作物による科学コミュニケーション・2019.12版
創作物による科学コミュニケーション・2019.12版創作物による科学コミュニケーション・2019.12版
創作物による科学コミュニケーション・2019.12版2co
 
細胞農業の社会受容:GMOからの教訓
細胞農業の社会受容:GMOからの教訓細胞農業の社会受容:GMOからの教訓
細胞農業の社会受容:GMOからの教訓2co
 
DIYなまものづくりの展望
DIYなまものづくりの展望DIYなまものづくりの展望
DIYなまものづくりの展望2co
 
Maker Faire Tokyo 2019 「培養肉」
Maker Faire Tokyo 2019 「培養肉」Maker Faire Tokyo 2019 「培養肉」
Maker Faire Tokyo 2019 「培養肉」2co
 
培養液と培養肉のコスト試算について
培養液と培養肉のコスト試算について培養液と培養肉のコスト試算について
培養液と培養肉のコスト試算について2co
 
細胞農業時代の畜産業
細胞農業時代の畜産業細胞農業時代の畜産業
細胞農業時代の畜産業2co
 

More from 2co (20)

細胞農業の普及シナリオ
細胞農業の普及シナリオ細胞農業の普及シナリオ
細胞農業の普及シナリオ
 
New Harvest 2020 slides from Shojinmeat Project - Will there be a monopoly co...
New Harvest 2020 slides from Shojinmeat Project - Will there be a monopoly co...New Harvest 2020 slides from Shojinmeat Project - Will there be a monopoly co...
New Harvest 2020 slides from Shojinmeat Project - Will there be a monopoly co...
 
Cellular agriculture in space
Cellular agriculture in spaceCellular agriculture in space
Cellular agriculture in space
 
宇宙細胞農業
宇宙細胞農業宇宙細胞農業
宇宙細胞農業
 
EmTechAsia 2020 (postponed) Cell-based meat
EmTechAsia 2020 (postponed) Cell-based meatEmTechAsia 2020 (postponed) Cell-based meat
EmTechAsia 2020 (postponed) Cell-based meat
 
DIY細胞培養マニュアル
DIY細胞培養マニュアルDIY細胞培養マニュアル
DIY細胞培養マニュアル
 
純肉:細胞培養による食料生産(2020版)
純肉:細胞培養による食料生産(2020版)純肉:細胞培養による食料生産(2020版)
純肉:細胞培養による食料生産(2020版)
 
DIY cell culture manual (& the roadmap to DIY cell-based meat)
DIY cell culture manual (& the roadmap to DIY cell-based meat)DIY cell culture manual (& the roadmap to DIY cell-based meat)
DIY cell culture manual (& the roadmap to DIY cell-based meat)
 
Shojinmeat Project - Open source cellular agriculture initiative
Shojinmeat Project - Open source cellular agriculture initiativeShojinmeat Project - Open source cellular agriculture initiative
Shojinmeat Project - Open source cellular agriculture initiative
 
Roadmap to the Cellular Agriculture Era
Roadmap to the Cellular Agriculture EraRoadmap to the Cellular Agriculture Era
Roadmap to the Cellular Agriculture Era
 
The cellular agriculture farmscape
The cellular agriculture farmscapeThe cellular agriculture farmscape
The cellular agriculture farmscape
 
細胞農業の普及シナリオ
細胞農業の普及シナリオ細胞農業の普及シナリオ
細胞農業の普及シナリオ
 
植物肉・培養肉・AT Kearney社の分析レポート訳&まとめ
植物肉・培養肉・AT Kearney社の分析レポート訳&まとめ植物肉・培養肉・AT Kearney社の分析レポート訳&まとめ
植物肉・培養肉・AT Kearney社の分析レポート訳&まとめ
 
细胞农业的未来
细胞农业的未来细胞农业的未来
细胞农业的未来
 
創作物による科学コミュニケーション・2019.12版
創作物による科学コミュニケーション・2019.12版創作物による科学コミュニケーション・2019.12版
創作物による科学コミュニケーション・2019.12版
 
細胞農業の社会受容:GMOからの教訓
細胞農業の社会受容:GMOからの教訓細胞農業の社会受容:GMOからの教訓
細胞農業の社会受容:GMOからの教訓
 
DIYなまものづくりの展望
DIYなまものづくりの展望DIYなまものづくりの展望
DIYなまものづくりの展望
 
Maker Faire Tokyo 2019 「培養肉」
Maker Faire Tokyo 2019 「培養肉」Maker Faire Tokyo 2019 「培養肉」
Maker Faire Tokyo 2019 「培養肉」
 
培養液と培養肉のコスト試算について
培養液と培養肉のコスト試算について培養液と培養肉のコスト試算について
培養液と培養肉のコスト試算について
 
細胞農業時代の畜産業
細胞農業時代の畜産業細胞農業時代の畜産業
細胞農業時代の畜産業
 

Recently uploaded

WWW - World Wide Web presentation.pptx
WWW - World Wide Web   presentation.pptxWWW - World Wide Web   presentation.pptx
WWW - World Wide Web presentation.pptxdomainkillerlinux
 
MAGNET: Senior Thesis Storyboard Excerpt
MAGNET: Senior Thesis Storyboard ExcerptMAGNET: Senior Thesis Storyboard Excerpt
MAGNET: Senior Thesis Storyboard Excerptdcobb11
 
THE MEDIC, A STORY for entertainment.docx
THE MEDIC, A STORY for entertainment.docxTHE MEDIC, A STORY for entertainment.docx
THE MEDIC, A STORY for entertainment.docxazuremorn
 
Cabin by the azure lake, the story of a lady and a crocodile
Cabin by the azure lake, the story of a lady and a crocodileCabin by the azure lake, the story of a lady and a crocodile
Cabin by the azure lake, the story of a lady and a crocodileazuremorn
 
TREE VOLANOES, A STORY FOR ENTERTAINMENT
TREE VOLANOES, A STORY FOR ENTERTAINMENTTREE VOLANOES, A STORY FOR ENTERTAINMENT
TREE VOLANOES, A STORY FOR ENTERTAINMENTazuremorn
 
The Old Man and the Earthquake, a story for entertainment
The Old Man and the Earthquake, a story for entertainmentThe Old Man and the Earthquake, a story for entertainment
The Old Man and the Earthquake, a story for entertainmentazuremorn
 
MAGNET: Storyboard Excerpt Version Three
MAGNET: Storyboard Excerpt Version ThreeMAGNET: Storyboard Excerpt Version Three
MAGNET: Storyboard Excerpt Version Threedcobb11
 
Mince Pies, a story for entertainment.docx
Mince Pies, a story for entertainment.docxMince Pies, a story for entertainment.docx
Mince Pies, a story for entertainment.docxazuremorn
 
The lady in Surtout, an old story that happened in our neighbourhood
The lady in Surtout, an old story  that happened in our neighbourhoodThe lady in Surtout, an old story  that happened in our neighbourhood
The lady in Surtout, an old story that happened in our neighbourhoodazuremorn
 
Green minimalist professional Business Proposal Presentation.pdf
Green minimalist professional Business Proposal Presentation.pdfGreen minimalist professional Business Proposal Presentation.pdf
Green minimalist professional Business Proposal Presentation.pdfrudkiaman0001
 
karaoke songs customise for portable 2.docx
karaoke songs customise for portable  2.docxkaraoke songs customise for portable  2.docx
karaoke songs customise for portable 2.docxPOPCOMTABUKCITY
 
Bald Philosopher, a story for entertainment.docx
Bald Philosopher, a story for entertainment.docxBald Philosopher, a story for entertainment.docx
Bald Philosopher, a story for entertainment.docxazuremorn
 
Transform Your Space with Poster Memorabilia's Collection
Transform Your Space with Poster Memorabilia's CollectionTransform Your Space with Poster Memorabilia's Collection
Transform Your Space with Poster Memorabilia's CollectionPoster Memorabilia Reviews
 

Recently uploaded (13)

WWW - World Wide Web presentation.pptx
WWW - World Wide Web   presentation.pptxWWW - World Wide Web   presentation.pptx
WWW - World Wide Web presentation.pptx
 
MAGNET: Senior Thesis Storyboard Excerpt
MAGNET: Senior Thesis Storyboard ExcerptMAGNET: Senior Thesis Storyboard Excerpt
MAGNET: Senior Thesis Storyboard Excerpt
 
THE MEDIC, A STORY for entertainment.docx
THE MEDIC, A STORY for entertainment.docxTHE MEDIC, A STORY for entertainment.docx
THE MEDIC, A STORY for entertainment.docx
 
Cabin by the azure lake, the story of a lady and a crocodile
Cabin by the azure lake, the story of a lady and a crocodileCabin by the azure lake, the story of a lady and a crocodile
Cabin by the azure lake, the story of a lady and a crocodile
 
TREE VOLANOES, A STORY FOR ENTERTAINMENT
TREE VOLANOES, A STORY FOR ENTERTAINMENTTREE VOLANOES, A STORY FOR ENTERTAINMENT
TREE VOLANOES, A STORY FOR ENTERTAINMENT
 
The Old Man and the Earthquake, a story for entertainment
The Old Man and the Earthquake, a story for entertainmentThe Old Man and the Earthquake, a story for entertainment
The Old Man and the Earthquake, a story for entertainment
 
MAGNET: Storyboard Excerpt Version Three
MAGNET: Storyboard Excerpt Version ThreeMAGNET: Storyboard Excerpt Version Three
MAGNET: Storyboard Excerpt Version Three
 
Mince Pies, a story for entertainment.docx
Mince Pies, a story for entertainment.docxMince Pies, a story for entertainment.docx
Mince Pies, a story for entertainment.docx
 
The lady in Surtout, an old story that happened in our neighbourhood
The lady in Surtout, an old story  that happened in our neighbourhoodThe lady in Surtout, an old story  that happened in our neighbourhood
The lady in Surtout, an old story that happened in our neighbourhood
 
Green minimalist professional Business Proposal Presentation.pdf
Green minimalist professional Business Proposal Presentation.pdfGreen minimalist professional Business Proposal Presentation.pdf
Green minimalist professional Business Proposal Presentation.pdf
 
karaoke songs customise for portable 2.docx
karaoke songs customise for portable  2.docxkaraoke songs customise for portable  2.docx
karaoke songs customise for portable 2.docx
 
Bald Philosopher, a story for entertainment.docx
Bald Philosopher, a story for entertainment.docxBald Philosopher, a story for entertainment.docx
Bald Philosopher, a story for entertainment.docx
 
Transform Your Space with Poster Memorabilia's Collection
Transform Your Space with Poster Memorabilia's CollectionTransform Your Space with Poster Memorabilia's Collection
Transform Your Space with Poster Memorabilia's Collection
 

DIY culture medium manual for DMEM and L15

  • 1. DIY culture media for kitchen experiments
 1
  • 2. Background 1: food safe DIY culture In past experiments, “Green Dakara” sports drink could be blended with DMEM up to 60% without losing cell culture efficacy. https://www.slideshare.net/2co/shojinmeat-project-cellular-agriculture-initiative-nov2018 However, all ingredients must be replaced with commonly available materials to enable kitchens DIY cell culture experiments. ※Research grade reagents = Not approved as food and not commonly available. Food or food additives that can be ingested (within limits) are commonly available. Basal media (DMEM, L15) was prepared entirely from commonly available materials and a DIY cell culture was demonstrated.
  • 3. Background 2: composition of DMEM and L15 The biggest difference between DMEM and L15 is in the minerals. Other ingredients were assumed common. Precisely, DMEM and L15 have different amino acid compositions and DMEM uses glucose while L15 uses galactose DMEM⇒https://www.sigmaaldrich.com/life-science/cell-culture/learning-center/media-formulations/dme.html L15⇒ https://www.sigmaaldrich.com/life-science/cell-culture/learning-center/media-formulations/l-15.html DMEM L15 20 amino acids 20 amino acids Water- soluble vitamin B’s Water- soluble vitamin B’s Glucose (dextrose) Glucose (dextrose) CaCl2, MgSO4, KCl, NaCl, NaHCO3, NaH2PO4, Iron nitrate CaCl2, MgCl2, MgSO4, KCl, NaCl, KH2PO4, Na2HPO4
  • 4. Ingredient 1 (Sugar, animo acids, vitamin B) 0.32g per tablet 0.22g amino acid (+sugar & fiber) 1 tablet (0.2g, incl. shellac shell) for 2L. Glucose for baking - Use 4.5g/L https://item.rakuten.co.jp/fancl-shop/5272/?i asid=07rpp_10095___e4-jpeuuk00-aev-615 07def-77bf-41df-8809-18631a826757 https://item.rakuten.co.jp/ kenkocom/x252760h/ https://item.rakuten.co.jp/a uc-garlic/budoutou-3000/
  • 5. Ingredient 2 (Minerals for DMEM) https://item.rakuten.co.jp/m amoru/4571474270191/ https://item.rakuten.co.jp/m atsubayakuhin/10000000/ CaCl2 (calcium chloride) NaCl(salt) https://item.rakuten.co.jp/ jyugo/t-4987306019126/ Half Salt NaCl/KCl mixed at 1:1 MgSO4  (Magnesium sulfate) Mixing at 1:1 molar quantity makes NaH2PO4 ※Iron nitrate not included ・Only miniscule amount is needed. ・Iron nitrate is not commonly available - not a food additive. ・Replaceable with iron (III) chloride? baking soda https://www.amazon.co.jp/%E 3%82%AA%E3%83%AB%E3 %83%88%E3%83%AA%E3% 83%B3%E9%85%B8-%E3%8 3%AA%E3%83%B3%E9%85 %B8%EF%BC%98%EF%BC %95%EF%BC%85%E3%80% 8C%E9%A3%9F%E5%93%81 %E6%B7%BB%E5%8A%A0% E7%89%A9%E3%80%8D%E F%BC%95%EF%BC%90%EF %BC%90%EF%BD%87/dp/B0 062HL4PU phosphoric acid
  • 6. Ingredient 2 (Minerals for L15) https://item.rakuten.co.jp/ mamoru/4571474270177/ MgCl2  (magnesium chloride) https://item.rakuten.co.jp/ kurimoto/100001011/ NaCl(salt) https://item.rakuten.co.jp/ jyugo/t-4987306019126/ Half salt 1:1 NaCl/KCl mixture https://www.amazon.co.jp/%E 3%82%AA%E3%83%AB%E3 %83%88%E3%83%AA%E3% 83%B3%E9%85%B8-%E3%8 3%AA%E3%83%B3%E9%85 %B8%EF%BC%98%EF%BC %95%EF%BC%85%E3%80% 8C%E9%A3%9F%E5%93%81 %E6%B7%BB%E5%8A%A0% E7%89%A9%E3%80%8D%E F%BC%95%EF%BC%90%EF %BC%90%EF%BD%87/dp/B0 062HL4PU Mixing at 1:1 molar quantity makes NaH2PO4 https://item.rakuten.co.jp/m amoru/4571474270191/ MgSO4  (Magnesium sulfate) potassium carbonate Mixing at 1:1 molar quantity makes KH2PO4 baking soda phosphoric acid
  • 7. 1X Solution 20X Solution Synthesis method DMEM: Table salt 0.58g baking soda 0.37g Half salt 0.13g L15: Table NaCl 0.95g Half salt 0.10g 125ml water 1 amino acid tablet + 99ml water 1 vitamin B pill General strategy for preparation ・Prepare 1X concentrated solution for salts, glucose, and amino acid, for which large quantity is needed. ・Prepare 20X solution for vitamin B’s and other minerals, for which only trace amount is needed. ・Prepare phosphates from phosphoric acid and mineral salts. DMEM: CaCl2 0.50g MgSO4 0.25g baking soda 0.19g Phosphoric acid diluted 10X 1.4ml L15: CaCl2 0.28g MgSO4 0.20g MgCl2 0.19g K2CO3 0.06g Phosphoric acid diluted 10X 1.4ml Mix 1X solution and 20x solution by 19:1, add glucose at 4.5g/L and bon apetit
  • 8. Scenes from the experiment ・Filtration is needed, as the tablet excipients need to be removed. ・Wait until the suspension settles to avoid clogging the filter paper. Holding position with a peeler Filtered 1X solution
  • 9. Cell culture demonstration Primary culture of chicken foetal heart cells by DIY-DMEM: Successful cell adhesion and proliferation (culturing experiment by @thesow41 on twitter) https://www.nicovideo.jp/watch/sm33104702 ※DIY-L15 to be tested
  • 10. Participate in improving this manual! https://docs.google.com/presentation/d/1cNedlmZcW7JOKB-gM itL3aIix1YXd5gi0hhlYz1mVqQ/edit#slide=id.g5683e89615_0_0 Source file openly accessible and editable