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DNA Barcoding
Travis Butler, Crystal Dobson, James Gainous, Andres Gomez and Xegfred Quidet
The Pressing Issues
Agriculture
● Food imports account for 26.6%
(2019)
● Food Import - $18 million USD
Annually
● $2 million USD - Onions (2020)
● $90,000 USD - Avocados (2020)
Access to Herbal Meds
● Many islanders opt for herbal
medicine as an alternative to
pharmaceuticals.
● Understanding the availability and
diversity of medicinal plant life is
essential to improving quality of
island life.
Goal:
● Locate and sequence beneficial organisms
● Trace to any possible agriculture and/or medicinal usages
○ Identify if the soil could grow certain plants for food and or/ medicinal
properties based on the current plants that can grow in the environment
■ Ex. Vetiver Grass and young avocado trees
The Process and Methods
3/18
● Present
Project Plan
● Receive plot
assignment
● Collect plant samples at
assigned plot
● Morphogenic analysis of
collected plant species
● DNA extraction
● PCR
● Gel electrophoresis
and DNA
sequencing
● BLAST sequencing
● Morphogenic
analysis of collected
plant species
● Bioinformatics
3/25 3/26 3/27
4/10
● Presentation
4/29
Collection
DNA Extraction
Polymerase Chain
Reaction (PCR)
Gel Electrophoresis
DNA sequencing and
Nucleotide Blasting
Collection Pictures- Chionanthus
compactus- “Bridgo Tree”
- Family: Oleaceae (Olive Family)
- No known agriculture /
medicinal benefits
- Locations: Antigua, Saba, St.
Eustatius, St. Kitts, Nevis,
Montserrat, Guadeloupe, La
Désirade, Marie Galante,
Dominica, Martinique, St. Lucia,
St. Vincent, the Grenadines,
Grenada, Greater Antilles
(Hispaniola, Puerto Rico), Virgin
Islands, Trinidad, South
America.
Collection Picture- Guettarda elliptica-
“Hammock Velvetseed”
- No known medicinal
benefits
- Agriculture:
- Often used as a
source of wood or a
“natural fence”
around property in
tropical areas
- Wood can be used
for fuel
Collection Pictures- Genus Pitraea
Members of the genus are
thought to be used as an
anti-inflammatory and
analgesic.
May also be used to treat
flu symptoms.
Collection Pictures-Scutia
myrtina- “Cat Thorn”
1. Laxative
2. Antimalarial
3. CNS Depression
4. Analgesic
5. Antidiabetic
6. Antioxidant
7. Anti-inflammatory
8. Anti-tumor
Petrea Kohautiana: (native)
Queen’s Wreath, Sandpiper Vine, Purple Wreath, Nilmani
*Due to insufficient published literature on Kohatiana, the following information pertains to Volubilis*
Agricultural Use:
● Floriculture
● Support bee
hives
● Animal feed
Medicinal Sap/Extract Use:
● Wound healing (Wayapi anecdote)
● Anti-inflammatory (Wayapi anecdote)
● Antidiarrheal (Wayapi anecdote)
● Abortifacient tea (Wayapi anecdote)
● Antidiabetic (mice)
● Antioxidant
● Anticancer
● Antimicrobial
Mimosa Pudica: (native)
Shame Plant, Sensitive Plant, Sleepy Plant, Action Plant, Touch-Me-Not
Medicinal Extract Use:
● Wound healing (topical:rats)
● PNS regeneration (IV:rats)
● Antidepressant (IV:rats)
● Anticonvulsant (intraperitoneal:mice)
● Diuretic (oral:rats, dogs)
● Birth control (oral:mice)
● Antioxidant (oral:mice, rats)
● Antivenom
● antimicrobial
● Aphrodisiac (oral:mice)
Agricultural Use:
● Legume: nodules facilitate growth of nitrogen fixing
bacteria
● Synthesize carbon disulfide: prevent pathologic fungus
growth in soil
● Phytoremediation
Collection Pictures-Euphorbia tithymaloides
(L.)/Pedilanthus tithymaloides “Devils Backbone”
● Potentially invasive species
● Can grow in toxic soils
(occasionally used clean soil)
● Being investigated for a biolfuel
● Potentially toxic properties
(euphorbol, terpene,
cycloartenone, beta-sitosterol)
● N/V/D, conjunctivitis, blistering,
pain.
Unconfirmed medical uses:
● emetic
● anti-inflammatory
● anti-parasitic
● Asthma
● Calluses
● Diabetes
● Anti-fungal
Phylogeny
Tree
Next Steps …
● Genus Pitraea, Petrea Kohautiana & Scutia myrtina-
Explore simpler methods for anti-inflammatory and
analgesic refinement
● Mimosa Pudica- Explore agricultural and medicinal
usage for fungal prevention
● Euphorbia tithymaloides- Explore purifying properties
Acknowledgements:
 Financial Support from AUACOM
 Dept. of Environment for giving us access to Walling’s Nature Reserve
 AUA professors who organize and mentored in this program
QUESTIONS?
1. http://aua-dna-barcoding.weebly.com/2019-project-results.html
2. https://en.wikipedia.org/wiki/Euphorbia_tithymaloides
3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459453/#:~:text=It%20majorly%20possesses%20antibacterial
%2C%20antivenom,and%20also%20applied%20on%20wounds.
4. https://en.wikipedia.org/wiki/Scutia_myrtina
5. https://www.eattheweeds.com/tag/queens-
wreath/#:~:text=An%20extract%20of%20its%20leaves,diabetes%20and%20high%20blood%20press
ure.
6. https://www.saintlucianplants.com/floweringplants/oleaceae/chiocomp/chiocomp.html
7. Bueno Dos Reis, Fábio (2010). "Nodulation and Nitrogen Fixation by Mimosa spp. in the Cerrado and
Caatinga Biomes of Brazil". New Phytologist. 186 (4): 934–946. doi:10.1111/j.1469-8137.2010.03267
8. Visoottiviseth, P; Francesconi, K; Sridokchan, W (August 2002). "The potential of Thai indigenous
plant species for the phytoremediation of arsenic contaminated land". Environmental Pollution. 118
(3): 453–461. doi:10.1016/S0269-7491(01)00293-7
9. Petrea volubilis L. Verbenaceae Tropical Plants Database, Ken Fern. tropical.theferns.info
10. http://www.stuartxchange.org/PurpleWreath
11. https://www.ijsr.net/archive/v4i4/SUB152754.pdf
12. http://www.jofamericanscience.org/journals/am-sci/am0808/031_9893am0808_202_208.pdf
13. https://pubmed.ncbi.nlm.nih.gov/22435170/
14. https://www.cabi.org/isc/datasheet/39815#touses
References

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C. DNA Barcoding Finale.pptx

  • 1. DNA Barcoding Travis Butler, Crystal Dobson, James Gainous, Andres Gomez and Xegfred Quidet
  • 2. The Pressing Issues Agriculture ● Food imports account for 26.6% (2019) ● Food Import - $18 million USD Annually ● $2 million USD - Onions (2020) ● $90,000 USD - Avocados (2020) Access to Herbal Meds ● Many islanders opt for herbal medicine as an alternative to pharmaceuticals. ● Understanding the availability and diversity of medicinal plant life is essential to improving quality of island life.
  • 3. Goal: ● Locate and sequence beneficial organisms ● Trace to any possible agriculture and/or medicinal usages ○ Identify if the soil could grow certain plants for food and or/ medicinal properties based on the current plants that can grow in the environment ■ Ex. Vetiver Grass and young avocado trees
  • 4. The Process and Methods 3/18 ● Present Project Plan ● Receive plot assignment ● Collect plant samples at assigned plot ● Morphogenic analysis of collected plant species ● DNA extraction ● PCR ● Gel electrophoresis and DNA sequencing ● BLAST sequencing ● Morphogenic analysis of collected plant species ● Bioinformatics 3/25 3/26 3/27 4/10 ● Presentation 4/29
  • 8. Gel Electrophoresis DNA sequencing and Nucleotide Blasting
  • 9. Collection Pictures- Chionanthus compactus- “Bridgo Tree” - Family: Oleaceae (Olive Family) - No known agriculture / medicinal benefits - Locations: Antigua, Saba, St. Eustatius, St. Kitts, Nevis, Montserrat, Guadeloupe, La Désirade, Marie Galante, Dominica, Martinique, St. Lucia, St. Vincent, the Grenadines, Grenada, Greater Antilles (Hispaniola, Puerto Rico), Virgin Islands, Trinidad, South America.
  • 10. Collection Picture- Guettarda elliptica- “Hammock Velvetseed” - No known medicinal benefits - Agriculture: - Often used as a source of wood or a “natural fence” around property in tropical areas - Wood can be used for fuel
  • 11. Collection Pictures- Genus Pitraea Members of the genus are thought to be used as an anti-inflammatory and analgesic. May also be used to treat flu symptoms.
  • 12. Collection Pictures-Scutia myrtina- “Cat Thorn” 1. Laxative 2. Antimalarial 3. CNS Depression 4. Analgesic 5. Antidiabetic 6. Antioxidant 7. Anti-inflammatory 8. Anti-tumor
  • 13. Petrea Kohautiana: (native) Queen’s Wreath, Sandpiper Vine, Purple Wreath, Nilmani *Due to insufficient published literature on Kohatiana, the following information pertains to Volubilis* Agricultural Use: ● Floriculture ● Support bee hives ● Animal feed Medicinal Sap/Extract Use: ● Wound healing (Wayapi anecdote) ● Anti-inflammatory (Wayapi anecdote) ● Antidiarrheal (Wayapi anecdote) ● Abortifacient tea (Wayapi anecdote) ● Antidiabetic (mice) ● Antioxidant ● Anticancer ● Antimicrobial
  • 14. Mimosa Pudica: (native) Shame Plant, Sensitive Plant, Sleepy Plant, Action Plant, Touch-Me-Not Medicinal Extract Use: ● Wound healing (topical:rats) ● PNS regeneration (IV:rats) ● Antidepressant (IV:rats) ● Anticonvulsant (intraperitoneal:mice) ● Diuretic (oral:rats, dogs) ● Birth control (oral:mice) ● Antioxidant (oral:mice, rats) ● Antivenom ● antimicrobial ● Aphrodisiac (oral:mice) Agricultural Use: ● Legume: nodules facilitate growth of nitrogen fixing bacteria ● Synthesize carbon disulfide: prevent pathologic fungus growth in soil ● Phytoremediation
  • 15. Collection Pictures-Euphorbia tithymaloides (L.)/Pedilanthus tithymaloides “Devils Backbone” ● Potentially invasive species ● Can grow in toxic soils (occasionally used clean soil) ● Being investigated for a biolfuel ● Potentially toxic properties (euphorbol, terpene, cycloartenone, beta-sitosterol) ● N/V/D, conjunctivitis, blistering, pain. Unconfirmed medical uses: ● emetic ● anti-inflammatory ● anti-parasitic ● Asthma ● Calluses ● Diabetes ● Anti-fungal
  • 18. ● Genus Pitraea, Petrea Kohautiana & Scutia myrtina- Explore simpler methods for anti-inflammatory and analgesic refinement ● Mimosa Pudica- Explore agricultural and medicinal usage for fungal prevention ● Euphorbia tithymaloides- Explore purifying properties
  • 19. Acknowledgements:  Financial Support from AUACOM  Dept. of Environment for giving us access to Walling’s Nature Reserve  AUA professors who organize and mentored in this program
  • 21. 1. http://aua-dna-barcoding.weebly.com/2019-project-results.html 2. https://en.wikipedia.org/wiki/Euphorbia_tithymaloides 3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459453/#:~:text=It%20majorly%20possesses%20antibacterial %2C%20antivenom,and%20also%20applied%20on%20wounds. 4. https://en.wikipedia.org/wiki/Scutia_myrtina 5. https://www.eattheweeds.com/tag/queens- wreath/#:~:text=An%20extract%20of%20its%20leaves,diabetes%20and%20high%20blood%20press ure. 6. https://www.saintlucianplants.com/floweringplants/oleaceae/chiocomp/chiocomp.html 7. Bueno Dos Reis, Fábio (2010). "Nodulation and Nitrogen Fixation by Mimosa spp. in the Cerrado and Caatinga Biomes of Brazil". New Phytologist. 186 (4): 934–946. doi:10.1111/j.1469-8137.2010.03267 8. Visoottiviseth, P; Francesconi, K; Sridokchan, W (August 2002). "The potential of Thai indigenous plant species for the phytoremediation of arsenic contaminated land". Environmental Pollution. 118 (3): 453–461. doi:10.1016/S0269-7491(01)00293-7 9. Petrea volubilis L. Verbenaceae Tropical Plants Database, Ken Fern. tropical.theferns.info 10. http://www.stuartxchange.org/PurpleWreath 11. https://www.ijsr.net/archive/v4i4/SUB152754.pdf 12. http://www.jofamericanscience.org/journals/am-sci/am0808/031_9893am0808_202_208.pdf 13. https://pubmed.ncbi.nlm.nih.gov/22435170/ 14. https://www.cabi.org/isc/datasheet/39815#touses References

Editor's Notes

  1. Xeg
  2. Crystal
  3. Crystal
  4. Xeg
  5. Xeg
  6. Xeg
  7. Crystal [4] https://www.saintlucianplants.com/floweringplants/oleaceae/chiocomp/chiocomp.html It is apart of the olive family so further research would be great to see if they could contribute to agriculture in supporting the growth of olive trees if planted her in antigua! No known agricultural or medicinal benefits but we do know it grows in the caribbean
  8. http://tropical.theferns.info/viewtropical.php?id=Guettarda+scabra Crystal
  9. https://www.eattheweeds.com/tag/queens-wreath/#:~:text=An%20extract%20of%20its%20leaves,diabetes%20and%20high%20blood%20pressure. Travis
  10. http://aua-dna-barcoding.weebly.com/2019-project-results.html Travis
  11. https://www.cabi.org/isc/datasheet/39815#touses https://pubmed.ncbi.nlm.nih.gov/22435170/ http://www.jofamericanscience.org/journals/am-sci/am0808/031_9893am0808_202_208.pdf https://www.ijsr.net/archive/v4i4/SUB152754.pdf http://www.stuartxchange.org/PurpleWreath Petrea volubilis L. Verbenaceae Tropical Plants Database, Ken Fern. tropical.theferns.info http://nature.jardin.free.fr/1102/nmauric_petrea_volubilis.html C7: Andy
  12. Visoottiviseth, P; Francesconi, K; Sridokchan, W (August 2002). "The potential of Thai indigenous plant species for the phytoremediation of arsenic contaminated land". Environmental Pollution. 118 (3): 453–461. doi:10.1016/S0269-7491(01)00293-7 Bueno Dos Reis, Fábio (2010). "Nodulation and Nitrogen Fixation by Mimosa spp. in the Cerrado and Caatinga Biomes of Brazil". New Phytologist. 186 (4): 934–946. doi:10.1111/j.1469-8137.2010.03267 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459453/#:~:text=It%20majorly%20possesses%20antibacterial%2C%20antivenom,and%20also%20applied%20on%20wounds. C8: Andy
  13. James
  14. James