SlideShare a Scribd company logo
1 of 10
Automatic Stainer &
Screener
What is Staining?
Most tissues, specially animal tissues, are colorless.
Dyes are colored substances with affinity for specific
molecules of tissues, so they get attached to the tissue and
provide color.
Staining is the process of coloring tissues by using dyes.
It allows visualizing cells and extracellular matrix to be
studied with light microscopes.
Staining is usually done in tissue sections and cell smears.
The most common sections for staining are those obtained
from paraffin embedding and frozen tissues.
A stain is a mixture of dyes that enhance the contrast of
the microscopic image. In general, most cells that make up
tissues are colorless and transparent. Therefore, tissues are
stained in such a way to make the cells visible.
Each dye within the stain selectively highlights a
particular chemical entity within a tissue.
 Therefore, several components of the tissue can be in
different colors. Hence, stains give a contrast to the
microscopic slide.
A dye is a single chemical component contained in a
stain. A single dye can highlight a specific type of
chemical entity within the tissue.
Stain examples- H & E
Haemotoxylin stains certain parts of the cell – like
the nucleus – blue
Eosin stains other parts of the cell – such as
the cytoplasm – red or pink.
Congo red,Crystal Violet, Giemsa, Aldehyde fuchsin
Dyes Examples-
Methylene blue, toluidine blue, thionine, carmine, basic
fuchsin, and azure II, aniline blue e.t.c.
Tissue sample stain by stain Tissue sample stain by dye
What is Manual Staining?
Manually staining slides allows pathologists to gain a full
understanding of the principles and processes of each
staining protocol.
Technicians can use this knowledge to their advantage by
controlling steps; making slight adjustments based on
judgment and personal experience to achieve the desired
outcome.
When staining by hand you exert much more force
dipping the slide into the reagent. The force can vary
between each slide/rack and can enter the reagent from
different angles subject to how the reagent looks.
 The extra force causes the reagent to bombard the slide,
resulting in the tension of the reagent on the slide to break
quicker than it would by a machine and then replaced by
the next reagent.
Manual staining also allows you to knock off reagent so
less reagent clings to the slide, decreasing the consumption
of costly reagents.
But problem is that laboratorians are not equally skilled,
and therefore standardization is impossible.
What is Automatic Staining?
During staining procedures each slide has to be
individually stained and kept for the prescribed amount of
time.
 Each step of staining has to be performed manually.
We have to pay attention to each slide and keep a track of
time and the stains added.
 There is a chance of the slides being over stained if the
incubation time is exceeded or not stained enough if
washed of sooner than required.
 Staining of multiple slides together takes a lot of time
and labour.
 Thus the process of staining is tedious and time
consuming. This calls for the need of : An automated
staining device.
Automatic Tissue Stainner?
A device that stains our slides automatically.
 Just enter the slide with the culture heat fixed onto it into
the machine.
 Load the various stains and reagents required in the
protocol in the right order.
 Select the required staying time of each stain or reagent
and the washes to be given in between steps etc. on the
digital display of the machine.
 Since the device is fully automated the only thing we
need to add are the various stains and reagents.
 We can also load multiple heat fixed slides which have to
be stained in the same manner. Selecting and setting the
right protocol and time on the display is very important.
Automatic Stainer & Screener technique.pptx

More Related Content

Similar to Automatic Stainer & Screener technique.pptx

Paper Con 2011 Presentation Continuous Coloration Of Coatings For Coated Pa...
Paper Con 2011 Presentation   Continuous Coloration Of Coatings For Coated Pa...Paper Con 2011 Presentation   Continuous Coloration Of Coatings For Coated Pa...
Paper Con 2011 Presentation Continuous Coloration Of Coatings For Coated Pa...Mark Crable
 
Wet Testing of textile materials
Wet Testing of textile materialsWet Testing of textile materials
Wet Testing of textile materialsAzmir Latif Beg
 
Presentation Comparative Study Between Reactive (Hot Brand) & Pigment Printed...
Presentation Comparative Study Between Reactive (Hot Brand) & Pigment Printed...Presentation Comparative Study Between Reactive (Hot Brand) & Pigment Printed...
Presentation Comparative Study Between Reactive (Hot Brand) & Pigment Printed...suman mazumder
 
Fault of knitted fabrics
Fault of knitted fabricsFault of knitted fabrics
Fault of knitted fabricsAzmir Latif Beg
 
City University textile department, topic: cotton dyeing method with reactive...
City University textile department, topic: cotton dyeing method with reactive...City University textile department, topic: cotton dyeing method with reactive...
City University textile department, topic: cotton dyeing method with reactive...Anik Deb
 
TCP-PSG-Dyeing.ppt
TCP-PSG-Dyeing.pptTCP-PSG-Dyeing.ppt
TCP-PSG-Dyeing.pptghoshb
 
Innovative Concepts in Textile Dyeing and Printing
Innovative Concepts in Textile Dyeing and PrintingInnovative Concepts in Textile Dyeing and Printing
Innovative Concepts in Textile Dyeing and PrintingVijay Prakash
 
Tissue processing 2012
Tissue processing 2012Tissue processing 2012
Tissue processing 2012Dhiraj Shukla
 
Introduction to dyeing
Introduction to dyeingIntroduction to dyeing
Introduction to dyeingRajeev Sharan
 
60502_Staining of peripheral blood film.pdf
60502_Staining of peripheral blood film.pdf60502_Staining of peripheral blood film.pdf
60502_Staining of peripheral blood film.pdfmohanak
 

Similar to Automatic Stainer & Screener technique.pptx (20)

TISSUE PROCESSING SEMINAR
TISSUE PROCESSING SEMINARTISSUE PROCESSING SEMINAR
TISSUE PROCESSING SEMINAR
 
Paper Con 2011 Presentation Continuous Coloration Of Coatings For Coated Pa...
Paper Con 2011 Presentation   Continuous Coloration Of Coatings For Coated Pa...Paper Con 2011 Presentation   Continuous Coloration Of Coatings For Coated Pa...
Paper Con 2011 Presentation Continuous Coloration Of Coatings For Coated Pa...
 
Wet Testing of textile materials
Wet Testing of textile materialsWet Testing of textile materials
Wet Testing of textile materials
 
right first time dyeing
right first time dyeingright first time dyeing
right first time dyeing
 
Presentation on Dyeing
Presentation on DyeingPresentation on Dyeing
Presentation on Dyeing
 
Staining
StainingStaining
Staining
 
Presentation Comparative Study Between Reactive (Hot Brand) & Pigment Printed...
Presentation Comparative Study Between Reactive (Hot Brand) & Pigment Printed...Presentation Comparative Study Between Reactive (Hot Brand) & Pigment Printed...
Presentation Comparative Study Between Reactive (Hot Brand) & Pigment Printed...
 
Fault of knitted fabrics
Fault of knitted fabricsFault of knitted fabrics
Fault of knitted fabrics
 
City University textile department, topic: cotton dyeing method with reactive...
City University textile department, topic: cotton dyeing method with reactive...City University textile department, topic: cotton dyeing method with reactive...
City University textile department, topic: cotton dyeing method with reactive...
 
Knit dyeing faults & Remedies
Knit dyeing faults & RemediesKnit dyeing faults & Remedies
Knit dyeing faults & Remedies
 
Batch to Batch shade variation
Batch to Batch shade variationBatch to Batch shade variation
Batch to Batch shade variation
 
TCP-PSG-Dyeing.ppt
TCP-PSG-Dyeing.pptTCP-PSG-Dyeing.ppt
TCP-PSG-Dyeing.ppt
 
module 1.pptx
module 1.pptxmodule 1.pptx
module 1.pptx
 
Innovative Concepts in Textile Dyeing and Printing
Innovative Concepts in Textile Dyeing and PrintingInnovative Concepts in Textile Dyeing and Printing
Innovative Concepts in Textile Dyeing and Printing
 
Staining in Microbiology
Staining in MicrobiologyStaining in Microbiology
Staining in Microbiology
 
Tissue processing 2012
Tissue processing 2012Tissue processing 2012
Tissue processing 2012
 
Foam Dyeing
Foam DyeingFoam Dyeing
Foam Dyeing
 
Introduction to dyeing
Introduction to dyeingIntroduction to dyeing
Introduction to dyeing
 
Histopathology.pptx
Histopathology.pptxHistopathology.pptx
Histopathology.pptx
 
60502_Staining of peripheral blood film.pdf
60502_Staining of peripheral blood film.pdf60502_Staining of peripheral blood film.pdf
60502_Staining of peripheral blood film.pdf
 

Recently uploaded

LESSON PLAN IN SCIENCE GRADE 4 WEEK 1 DAY 2
LESSON PLAN IN SCIENCE GRADE 4 WEEK 1 DAY 2LESSON PLAN IN SCIENCE GRADE 4 WEEK 1 DAY 2
LESSON PLAN IN SCIENCE GRADE 4 WEEK 1 DAY 2AuEnriquezLontok
 
CHROMATOGRAPHY PALLAVI RAWAT.pptx
CHROMATOGRAPHY  PALLAVI RAWAT.pptxCHROMATOGRAPHY  PALLAVI RAWAT.pptx
CHROMATOGRAPHY PALLAVI RAWAT.pptxpallavirawat456
 
Charateristics of the Angara-A5 spacecraft launched from the Vostochny Cosmod...
Charateristics of the Angara-A5 spacecraft launched from the Vostochny Cosmod...Charateristics of the Angara-A5 spacecraft launched from the Vostochny Cosmod...
Charateristics of the Angara-A5 spacecraft launched from the Vostochny Cosmod...Christina Parmionova
 
Pests of Sunflower_Binomics_Identification_Dr.UPR
Pests of Sunflower_Binomics_Identification_Dr.UPRPests of Sunflower_Binomics_Identification_Dr.UPR
Pests of Sunflower_Binomics_Identification_Dr.UPRPirithiRaju
 
The Sensory Organs, Anatomy and Function
The Sensory Organs, Anatomy and FunctionThe Sensory Organs, Anatomy and Function
The Sensory Organs, Anatomy and FunctionJadeNovelo1
 
Fertilization: Sperm and the egg—collectively called the gametes—fuse togethe...
Fertilization: Sperm and the egg—collectively called the gametes—fuse togethe...Fertilization: Sperm and the egg—collectively called the gametes—fuse togethe...
Fertilization: Sperm and the egg—collectively called the gametes—fuse togethe...D. B. S. College Kanpur
 
Observation of Gravitational Waves from the Coalescence of a 2.5–4.5 M⊙ Compa...
Observation of Gravitational Waves from the Coalescence of a 2.5–4.5 M⊙ Compa...Observation of Gravitational Waves from the Coalescence of a 2.5–4.5 M⊙ Compa...
Observation of Gravitational Waves from the Coalescence of a 2.5–4.5 M⊙ Compa...Sérgio Sacani
 
Oxo-Acids of Halogens and their Salts.pptx
Oxo-Acids of Halogens and their Salts.pptxOxo-Acids of Halogens and their Salts.pptx
Oxo-Acids of Halogens and their Salts.pptxfarhanvvdk
 
Explainable AI for distinguishing future climate change scenarios
Explainable AI for distinguishing future climate change scenariosExplainable AI for distinguishing future climate change scenarios
Explainable AI for distinguishing future climate change scenariosZachary Labe
 
projectile motion, impulse and moment
projectile  motion, impulse  and  momentprojectile  motion, impulse  and  moment
projectile motion, impulse and momentdonamiaquintan2
 
DECOMPOSITION PATHWAYS of TM-alkyl complexes.pdf
DECOMPOSITION PATHWAYS of TM-alkyl complexes.pdfDECOMPOSITION PATHWAYS of TM-alkyl complexes.pdf
DECOMPOSITION PATHWAYS of TM-alkyl complexes.pdfDivyaK787011
 
linear Regression, multiple Regression and Annova
linear Regression, multiple Regression and Annovalinear Regression, multiple Regression and Annova
linear Regression, multiple Regression and AnnovaMansi Rastogi
 
Forensic limnology of diatoms by Sanjai.pptx
Forensic limnology of diatoms by Sanjai.pptxForensic limnology of diatoms by Sanjai.pptx
Forensic limnology of diatoms by Sanjai.pptxkumarsanjai28051
 
Abnormal LFTs rate of deco and NAFLD.pptx
Abnormal LFTs rate of deco and NAFLD.pptxAbnormal LFTs rate of deco and NAFLD.pptx
Abnormal LFTs rate of deco and NAFLD.pptxzeus70441
 
Replisome-Cohesin Interfacing A Molecular Perspective.pdf
Replisome-Cohesin Interfacing A Molecular Perspective.pdfReplisome-Cohesin Interfacing A Molecular Perspective.pdf
Replisome-Cohesin Interfacing A Molecular Perspective.pdfAtiaGohar1
 
办理麦克马斯特大学毕业证成绩单|购买加拿大文凭证书
办理麦克马斯特大学毕业证成绩单|购买加拿大文凭证书办理麦克马斯特大学毕业证成绩单|购买加拿大文凭证书
办理麦克马斯特大学毕业证成绩单|购买加拿大文凭证书zdzoqco
 
DOG BITE management in pediatrics # for Pediatric pgs# topic presentation # f...
DOG BITE management in pediatrics # for Pediatric pgs# topic presentation # f...DOG BITE management in pediatrics # for Pediatric pgs# topic presentation # f...
DOG BITE management in pediatrics # for Pediatric pgs# topic presentation # f...HafsaHussainp
 
Combining Asynchronous Task Parallelism and Intel SGX for Secure Deep Learning
Combining Asynchronous Task Parallelism and Intel SGX for Secure Deep LearningCombining Asynchronous Task Parallelism and Intel SGX for Secure Deep Learning
Combining Asynchronous Task Parallelism and Intel SGX for Secure Deep Learningvschiavoni
 

Recently uploaded (20)

LESSON PLAN IN SCIENCE GRADE 4 WEEK 1 DAY 2
LESSON PLAN IN SCIENCE GRADE 4 WEEK 1 DAY 2LESSON PLAN IN SCIENCE GRADE 4 WEEK 1 DAY 2
LESSON PLAN IN SCIENCE GRADE 4 WEEK 1 DAY 2
 
CHROMATOGRAPHY PALLAVI RAWAT.pptx
CHROMATOGRAPHY  PALLAVI RAWAT.pptxCHROMATOGRAPHY  PALLAVI RAWAT.pptx
CHROMATOGRAPHY PALLAVI RAWAT.pptx
 
Charateristics of the Angara-A5 spacecraft launched from the Vostochny Cosmod...
Charateristics of the Angara-A5 spacecraft launched from the Vostochny Cosmod...Charateristics of the Angara-A5 spacecraft launched from the Vostochny Cosmod...
Charateristics of the Angara-A5 spacecraft launched from the Vostochny Cosmod...
 
Pests of Sunflower_Binomics_Identification_Dr.UPR
Pests of Sunflower_Binomics_Identification_Dr.UPRPests of Sunflower_Binomics_Identification_Dr.UPR
Pests of Sunflower_Binomics_Identification_Dr.UPR
 
The Sensory Organs, Anatomy and Function
The Sensory Organs, Anatomy and FunctionThe Sensory Organs, Anatomy and Function
The Sensory Organs, Anatomy and Function
 
Fertilization: Sperm and the egg—collectively called the gametes—fuse togethe...
Fertilization: Sperm and the egg—collectively called the gametes—fuse togethe...Fertilization: Sperm and the egg—collectively called the gametes—fuse togethe...
Fertilization: Sperm and the egg—collectively called the gametes—fuse togethe...
 
Observation of Gravitational Waves from the Coalescence of a 2.5–4.5 M⊙ Compa...
Observation of Gravitational Waves from the Coalescence of a 2.5–4.5 M⊙ Compa...Observation of Gravitational Waves from the Coalescence of a 2.5–4.5 M⊙ Compa...
Observation of Gravitational Waves from the Coalescence of a 2.5–4.5 M⊙ Compa...
 
Oxo-Acids of Halogens and their Salts.pptx
Oxo-Acids of Halogens and their Salts.pptxOxo-Acids of Halogens and their Salts.pptx
Oxo-Acids of Halogens and their Salts.pptx
 
Explainable AI for distinguishing future climate change scenarios
Explainable AI for distinguishing future climate change scenariosExplainable AI for distinguishing future climate change scenarios
Explainable AI for distinguishing future climate change scenarios
 
projectile motion, impulse and moment
projectile  motion, impulse  and  momentprojectile  motion, impulse  and  moment
projectile motion, impulse and moment
 
PLASMODIUM. PPTX
PLASMODIUM. PPTXPLASMODIUM. PPTX
PLASMODIUM. PPTX
 
DECOMPOSITION PATHWAYS of TM-alkyl complexes.pdf
DECOMPOSITION PATHWAYS of TM-alkyl complexes.pdfDECOMPOSITION PATHWAYS of TM-alkyl complexes.pdf
DECOMPOSITION PATHWAYS of TM-alkyl complexes.pdf
 
linear Regression, multiple Regression and Annova
linear Regression, multiple Regression and Annovalinear Regression, multiple Regression and Annova
linear Regression, multiple Regression and Annova
 
Let’s Say Someone Did Drop the Bomb. Then What?
Let’s Say Someone Did Drop the Bomb. Then What?Let’s Say Someone Did Drop the Bomb. Then What?
Let’s Say Someone Did Drop the Bomb. Then What?
 
Forensic limnology of diatoms by Sanjai.pptx
Forensic limnology of diatoms by Sanjai.pptxForensic limnology of diatoms by Sanjai.pptx
Forensic limnology of diatoms by Sanjai.pptx
 
Abnormal LFTs rate of deco and NAFLD.pptx
Abnormal LFTs rate of deco and NAFLD.pptxAbnormal LFTs rate of deco and NAFLD.pptx
Abnormal LFTs rate of deco and NAFLD.pptx
 
Replisome-Cohesin Interfacing A Molecular Perspective.pdf
Replisome-Cohesin Interfacing A Molecular Perspective.pdfReplisome-Cohesin Interfacing A Molecular Perspective.pdf
Replisome-Cohesin Interfacing A Molecular Perspective.pdf
 
办理麦克马斯特大学毕业证成绩单|购买加拿大文凭证书
办理麦克马斯特大学毕业证成绩单|购买加拿大文凭证书办理麦克马斯特大学毕业证成绩单|购买加拿大文凭证书
办理麦克马斯特大学毕业证成绩单|购买加拿大文凭证书
 
DOG BITE management in pediatrics # for Pediatric pgs# topic presentation # f...
DOG BITE management in pediatrics # for Pediatric pgs# topic presentation # f...DOG BITE management in pediatrics # for Pediatric pgs# topic presentation # f...
DOG BITE management in pediatrics # for Pediatric pgs# topic presentation # f...
 
Combining Asynchronous Task Parallelism and Intel SGX for Secure Deep Learning
Combining Asynchronous Task Parallelism and Intel SGX for Secure Deep LearningCombining Asynchronous Task Parallelism and Intel SGX for Secure Deep Learning
Combining Asynchronous Task Parallelism and Intel SGX for Secure Deep Learning
 

Automatic Stainer & Screener technique.pptx

  • 2. What is Staining? Most tissues, specially animal tissues, are colorless. Dyes are colored substances with affinity for specific molecules of tissues, so they get attached to the tissue and provide color. Staining is the process of coloring tissues by using dyes. It allows visualizing cells and extracellular matrix to be studied with light microscopes. Staining is usually done in tissue sections and cell smears. The most common sections for staining are those obtained from paraffin embedding and frozen tissues. A stain is a mixture of dyes that enhance the contrast of the microscopic image. In general, most cells that make up tissues are colorless and transparent. Therefore, tissues are stained in such a way to make the cells visible.
  • 3. Each dye within the stain selectively highlights a particular chemical entity within a tissue.  Therefore, several components of the tissue can be in different colors. Hence, stains give a contrast to the microscopic slide. A dye is a single chemical component contained in a stain. A single dye can highlight a specific type of chemical entity within the tissue. Stain examples- H & E Haemotoxylin stains certain parts of the cell – like the nucleus – blue Eosin stains other parts of the cell – such as the cytoplasm – red or pink. Congo red,Crystal Violet, Giemsa, Aldehyde fuchsin
  • 4. Dyes Examples- Methylene blue, toluidine blue, thionine, carmine, basic fuchsin, and azure II, aniline blue e.t.c. Tissue sample stain by stain Tissue sample stain by dye
  • 5. What is Manual Staining? Manually staining slides allows pathologists to gain a full understanding of the principles and processes of each staining protocol. Technicians can use this knowledge to their advantage by controlling steps; making slight adjustments based on judgment and personal experience to achieve the desired outcome. When staining by hand you exert much more force dipping the slide into the reagent. The force can vary between each slide/rack and can enter the reagent from different angles subject to how the reagent looks.
  • 6.  The extra force causes the reagent to bombard the slide, resulting in the tension of the reagent on the slide to break quicker than it would by a machine and then replaced by the next reagent. Manual staining also allows you to knock off reagent so less reagent clings to the slide, decreasing the consumption of costly reagents. But problem is that laboratorians are not equally skilled, and therefore standardization is impossible.
  • 7.
  • 8. What is Automatic Staining? During staining procedures each slide has to be individually stained and kept for the prescribed amount of time.  Each step of staining has to be performed manually. We have to pay attention to each slide and keep a track of time and the stains added.  There is a chance of the slides being over stained if the incubation time is exceeded or not stained enough if washed of sooner than required.  Staining of multiple slides together takes a lot of time and labour.  Thus the process of staining is tedious and time consuming. This calls for the need of : An automated staining device.
  • 9. Automatic Tissue Stainner? A device that stains our slides automatically.  Just enter the slide with the culture heat fixed onto it into the machine.  Load the various stains and reagents required in the protocol in the right order.  Select the required staying time of each stain or reagent and the washes to be given in between steps etc. on the digital display of the machine.  Since the device is fully automated the only thing we need to add are the various stains and reagents.  We can also load multiple heat fixed slides which have to be stained in the same manner. Selecting and setting the right protocol and time on the display is very important.