Targeted and Unbiased Screen for Genetic Suppressors of the Legionella pneumo...Chetan Hebbale
This document describes research into identifying genetic suppressors of toxicity caused by the Legionella pneumophila effector protein LegC7 in Saccharomyces cerevisiae. A targeted screen of Class E vacuolar protein sorting gene deletions did not suppress LegC7 toxicity except for vps27Δ. An unbiased ethyl methanesulfonate mutagenesis screen identified yeast strains where LegC7 toxicity was reversed, likely due to genomic or plasmid mutations. The genomes of two strains will be sequenced to find specific mutations, and an overexpression screen will search for genes that reduce LegC7 toxicity when overexpressed.
Targeted and Unbiased Screen for Genetic Suppressors of the Legionella pneumo...Chetan Hebbale
This document describes research into identifying genetic suppressors of toxicity caused by the Legionella pneumophila effector protein LegC7 in Saccharomyces cerevisiae. A targeted screen of Class E vacuolar protein sorting gene deletions did not suppress LegC7 toxicity except for vps27Δ. An unbiased ethyl methanesulfonate mutagenesis screen identified yeast strains where LegC7 toxicity was reversed, likely due to genomic or plasmid mutations. The genomes of two strains will be sequenced to find specific mutations, and an overexpression screen will search for genes that reduce LegC7 toxicity when overexpressed.