Daniel Greenfeld's talk at PyCon Poland 2012 focuses on the Zen of Python, emphasizing principles such as readability and simplicity. He also discusses the super() method's ambiguities, Django's class-based views, and distinguishes Django's Model-Template-View architecture from the traditional Model-View-Controller pattern. The presentation addresses practical challenges, such as error handling and namespace pollution in frameworks like web2py, while urging developers to adhere to Python's guiding principles.