SlideShare a Scribd company logo
Investigations on the incidence of bee losses in corn and
     oilseed rape growing areas of Austria and possible
  correlations with bee diseases and the use of insecticidal
                  plant protection products
                    (Acronym: MELISSA)
 Order for project: Federal Ministry of Agriculture, Forestry, the Environment and Water Management (BMLFUW)
   Funding: BMLFUW, Federal provinces of Austria, AGES, Consortium of seed and plant protection companies

                                          Duration: 1.3.2009 – 31.1.2012
                                                                              Details and full report: www.dafne.at
                                                                              Project Nr. 100472
Project manager: DI Leopold Girsch
Involved AGES-Divisions and Departments:
Division for Food Security: Inst. for Seed and Propagating Material, Phytosanitary Service and Apiculture;
                            Inst. for Sustainable Plant Production; Inst. for Plant Protection Products;
Division for Food Safety;
Department Data, Statistics & Risk Assessment

www.ages.at                                                   Austrian Agency for Health and Food Safety (AGES)
MELISSA

Objectives
• documentation of incidences of honey bee losses in production
  areas of maize and oilseed rape
• analysis of possible causes (honey bee pathogens and
  parasites, plant protection products)
• evaluation of results with respect to measures taken to prevent
  honey bee losses
• development of decision guidances for authorities, beekeepers
  and farmers for the implementation of measures to prevent
  honey bee losses by pathogens, parasites and plant protection
  products

                Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation:
www.ages.at                                                                                         2
                      Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012
Studies conducted

• diagnosis for pathogens and parasites in honey bees
 Varroa destructor, Nosema spp., 7 honey bee viruses

• analysis for residues
    - insecticidal seed coating products
    - other active substances of plant protection products

        sampled matrices:
                bees, beebread, extracted honey
                plants
                seed



                           Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation:
  www.ages.at                                                                                                  3
                                 Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012
Symptoms in honey bees and
bee colonies
     - increased numbers of dead bees inside and outside the hives
     - flightless bees with symptoms of trembling, cramping,
       disorientation, paralysis, abnormal wing movements,
       hyperexcitation, disordered locomotory activity
           o at time of maize sowing (simultaneously or from next day onwards)
           o ca. 10 – 14 days after beginning of maize sowing


     - flightless crawling bees or groups of bees on the soil or in the
       grass in front of beehives
               o   from the beginning of maize sowing
               o   in many cases also considerably later in the season (May until beginning of June)

     - intensity and duration of symptoms
               o one to several days – in some cases considerably longer
               o in some particular cases massive damages occured when       bees showed a
                   multiple contamination by insecticidal seed coating substances and other
                   insecticides
                                Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation:
 www.ages.at                                                                                                        4
                                      Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012
Observed symptoms in the apiaries
(1)
        • increased bee mortality




        Residue analysis in bees:                                                              Residue analysis in bees:
                 *Clothianidin 0.0021 mg/kg;                                                            *Clothianidin 0,0033 mg/kg;
                 *Thiamethoxam 0,0088 mg/kg                                                             *Imidacloprid 0,0273 mg/kg

                                                          *LOD = 0,0002 mg/kg; *LOQ = 0,001 mg/kg

Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                                                  5
Observed symptoms in the apiaries
(2)
• flightless crawling bees from the beginning of maize sowing and in many
  cases also considerably later in the season




                                                                                               Residue analysis:
Residue analysis:                                                                              bees: negative;
bees: negative                                                                                 bee bread positive: *Imidacloprid <
                                                                                               LOQ                 **Thiacloprid < LOQ
                               *LOD = 0,0002 mg/kg; *LOQ = 0,001 mg/kg; ** LOQ: 0,01 mg/kg
Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                                                     6
Observed symptoms in the apiaries
(3)
• large quantities of dead bees in cases of multiple contamination by insecticidal
  seed coating substances and other insecticides




Insecticides detected in dead bees by residue analysis: *Clothianidin, *Fipronil,
*Fipronil-sulfone, **Chlorpyrifos
                              *LOD = 0,0002 mg/kg; *LOQ = 0,001 mg/kg; ** LOQ: 0,01 mg/kg

Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                              7
Bee yards with suspected bee poisonig –
results of residue analysis year 2009

          Insecticidal seed coating materials: Clothianidin, Thiamethoxam,
          Imidacloprid, Fipronil and Fipronil-sulfone
                < LOQ
                ≥ LOQ
                negative




Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                              8
Bee yards with suspected bee poisonig –
results of residue analysis year 2010


   Insecticidal seed coating materials: Clothianidin, Thiamethoxam,
   Imidacloprid, Fipronil and Fipronil-sulfone
         < LOQ
         ≥ LOQ
         negative




Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                              9
Bee yards with suspected bee poisonig –
results of residue analysis year 2011


     Insecticidal seed coating materials: Clothianidin,
     Thiamethoxam, Imidacloprid, Fipronil and Fipronil-sulfone
             < LOQ
             ≥ LOQ
             negative




Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                              10
Results of residue analysis for
„other plant protection substances“

       Bees and Beebread
                – Several different active substances were detected
                         – insecticides
                         – herbicides
                         – fungicides
                – unapproved substances according to VO (EG) Nr.
                  1107/2009 were detected in single cases (e.g.
                  phosalone, chlorfenvinphos,paraoxon-methyl,
                  diphenylamin)


Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                              11
Colony losses during hibernation
period 2009/2010 (1)
    4 beekeepers supposed losses to be connected with the uptake of potentially
    contaminated pollen during late summer and autumn of the previous year

    a) Diagnostic results for pathogens and parasites
                  In beekeeping operations 1 – 3 Varroosis could be identified as the most likely cause for
                  colony losses (Varroa-infestation rate bee samples: 17-39 %; brood samples: 24-56 %)




                  In beekeeping operation 4 severe failures to comply with good apicultural practice were
                  revealed (establishment of very weak mating nucs late in the year, no protective measures
                  against shrews, dysentery and N. ceranae as cofactors), therefore no residue analysis was
                  performed
Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                              12
Colony losses during hibernation
period 2009/2010 (2)

    b) Residue analysis
             • No residues from insecticidal seed coating materials or
               other active substances of plant protection products were
               detected in
                      – bee bread samples (pooled by apiaries) from 3
                        beekeeping operations
                      – dead bees (pooled sample) from one beekeeping
                        operation
             • Conclusion: In these 4 cases there are no indications for
               a contamination of hive products by the investigated
               analytes
Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                              13
Colony losses during hibernation
period 2010/2011
6 beekeepers supposed losses to be connected with the uptake of potentially
contaminated pollen during late summer and autumn of the previous year

a) Residue analysis of bee bread from dead or very weak colonies
   • Case 1: Clothianidin < LOQ
   • Case 2: Imidacloprid < LOQ
   • Cases 3 - 5: negative
   • Case 6: no residue analysis was performed (severe case of varroosis: 24 % infestation
     rate in remaining brood cells)

b) Diagnostic results for pathogens and parasites
      In cases 3 - 6 pests and parasites were most likely the causes for colony winter losses.

Conclusions: an exposure to insecticide seed coating material was confirmed for 2
cases of winter losses. Because Imidacloprid is used both as a seed coating and a
spray treatment the source for this contaminant remained unclear.
Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                              14
Suspected bee poisoning incidents
during bloom of maize or later in
the year 2011
Reports from 5 beekeeping operations (5 bee yards)

Reasons suspected by beekeepers:

   • case 1: “spray applikations in seed maize or grape-vine“;
         residue analysis bees: Imidacloprid 0,0016 mg/kg
   • case 2: “plant protection activity“:
     residue analysis bees: Imidacloprid < LOQ (LOQ = 0,001 mg/kg)
   • cases 3, 4: “contaminated pollen from maize, oilseed-pumpkin, grape-vine“;
     residue analysis negative
   • case 5: “unidentified plant protection activity“; residue analysis not possible
     due to insufficient sample size

Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                              15
Clothianidin – three-years-comparison of
residue results for samples of bees and bee bread
                                             MELISSA-Vergiftungsverdacht
                            LOD           Rückstandsanalysen auf Clothianidin
                                          = 0,0002 mg/kg; LOQ = 0,001 mg/kg
                                               Matrix: Bienen und Bienenbrot
                                                                                                                                                                                        Legend:
                                                               CLO >= BG                   CLO < BG                    CLO n.n.                                                         BG = LOQ
    100%             0             0                                                                                                                                                    n.n. = not detected
      90%                                                                                         7
                                                                                                         15
                                                        2                                                       15
      80%
                                                                                                                               2
                                                                             11                   7      9
      70%                                                      19
                                                                                    28
      60%                                                                                                       9
                            18                                        42
                                                                                           29
      50%            1             3      1             2                                                                      0                    1      1

      40%                                                                    2

      30%                                                                                         24 42
                                                                7                    3                          27             2
      20%
                                                        2                    7                                                                                                         Bees + Bee bread:
      10%                   0
                                                                4
                                                                      5              9
                                                                                           1                                                                                           significant decline
                            2                                                              2
       0%     0      0                    0      0                    2                                                 0             0      0      0      0      0      0      0      in percentage of
              2009
                     2010
                            2011
                                   2009
                                          2010
                                                 2011
                                                        2009
                                                               2010
                                                                      2011
                                                                             2009
                                                                                    2010
                                                                                           2011
                                                                                                  2009
                                                                                                         2010
                                                                                                                2011
                                                                                                                        2009
                                                                                                                               2010
                                                                                                                                      2011
                                                                                                                                             2009
                                                                                                                                                    2010
                                                                                                                                                           2011
                                                                                                                                                                  2009
                                                                                                                                                                         2010
                                                                                                                                                                                2011
                                                                                                                                                                                       positive samples
                     B                    K                    NOE                  OOE                  ST                    T                    W                    V
                                                                                                                                                                                       from 2009 - 2011
Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                                                                                                                          16
Thiamethoxam – three-years-comparison of
residue results for samples of bees and bee
bread
                                      MELISSA-Vergiftungsverdacht
                               LODRückstandsanalysen auf Thiamethoxam
                                   = 0,0002 mg/kg; LOQ = 0,001 mg/kg
                                       Matrix: Bienen und Bienenbrot
                                                                                                                                                                                            Legend:
                                                                   THI >= BG                  THI < BG                THI n.n.                                                              BG = LOQ
        100%                          0                                                                                                                                                     n.n. = not detected
         90%

         80%

         70%
                                                                                                            44
         60%                                                                    16                   30
                                                           5 25                                                    44
                                                                                       36
         50%            1 20 3               1                           48                   31                                 4                    1      1

         40%

         30%
                                                                                                            12
         20%
                                                           0
                                                                  2             2                                                                                                        Bees + Bee bread:
         10%                                                                                         7         2
                                                           1
                                                                  3
                                                                         0
                                                                                2      3
                                                                                              1
                                                                                                            10
                                                                                                               5                                                                         significant decline in
           0%     0     0      0             0      0                    1             1      0      1                    0      0      0      0 0           0      0      0      0
                                                                                                                                                                                         percentage of positive
                 2009
                        2010
                               2011
                                      2009
                                             2010
                                                    2011
                                                           2009
                                                                  2010
                                                                         2011
                                                                                2009
                                                                                       2010
                                                                                              2011
                                                                                                     2009
                                                                                                            2010
                                                                                                                   2011
                                                                                                                          2009
                                                                                                                                 2010
                                                                                                                                        2011
                                                                                                                                               2009
                                                                                                                                                      2010
                                                                                                                                                             2011
                                                                                                                                                                    2009
                                                                                                                                                                           2010
                                                                                                                                                                                  2011
                                                                                                                                                                                         samples from 2009 -
                        B                    K                    NOE                  OOE                  ST                   T                    W                    V
                                                                                                                                                                                         2011
Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                                                                                                                              17
Fipronil + Fipronil-sulfone – three-years-
comparison of residue results for samples of
bees and bee bread
                                     MELISSA-Vergiftungsverdacht                                                                                                                                             MELISSA-Vergiftungsverdacht

                                                                  Fipronil
                                    Rückstandsanalysen auf Fipronil
                                      Matrix: Bienen und Bienenbrot
                                                                                                                                                                                                         Rückstandsanalysen auf Fipronil-sulfon
                                                                                                                                                                                                                       Fipronil-sulfone
                                                                                                                                                                                                              Matrix: Bienen und Bienenbrot

                                                    FIP >= BG                  FIP < BG                    FIP n.n.                                                                                                             FIP-S >= BG                    FIP-S < BG                  FIP-S n.n.

100%                                                                                                                                                                       100%

 90%                                                                                                                                                                       90%

 80%                                                                                                                                                                       80%

 70%                                                                                                                                                                       70%

 60%                                                                                                               3                                                       60%
                                                                                                                                                                                                                                                                                                   3

                                                   28                                        59                                                                                                                                    28                    62 50
 50%          1 20 3            1           6             49 20 38 32 36                            50                                  1      1                           50%           1 20 3               1             6             49 20 39 32 37                                                                1      1

 40%                                                                                                                                                                       40%

 30%                                                                                                                                                                       30%
                                                                                                                   0                                                                                                                                                                               0
 20%                                                                                                                                                                       20%
                                                                                                                   1                                                                                                                                                                               1
 10%                                                                                                                                                                       10%
                                                   0                                    4                                                                                                                                           0
                                                   2                    1             1 3           0                                                                                                                               2                                        2
 0%    0      0      0      0   0 0         0             0      0      1      0      1             1       0             0      0      0      0 0           0      0                                                                                    1            1      2      0
                                                                                                                                                                                                                                                                                    1
                                                                                                                                                                            0%    0      0      0      0      0      0      0             0      0       0     0      0                     0             0      0      0      0      0      0      0
       2009
              2010
                     2011
                            2009
                            2010
                                     2011
                                            2009
                                                   2010
                                                          2011
                                                                 2009
                                                                        2010
                                                                               2011
                                                                                      2009
                                                                                             2010
                                                                                                    2011
                                                                                                            2009
                                                                                                                   2010
                                                                                                                          2011
                                                                                                                                 2009
                                                                                                                                        2010
                                                                                                                                               2011
                                                                                                                                                      2009
                                                                                                                                                             2010
                                                                                                                                                                    2011




                                                                                                                                                                                  2009
                                                                                                                                                                                         2010
                                                                                                                                                                                                2011
                                                                                                                                                                                                       2009
                                                                                                                                                                                                              2010
                                                                                                                                                                                                                     2011
                                                                                                                                                                                                                            2009
                                                                                                                                                                                                                                   2010
                                                                                                                                                                                                                                          2011
                                                                                                                                                                                                                                                 2009
                                                                                                                                                                                                                                                        2010
                                                                                                                                                                                                                                                               2011
                                                                                                                                                                                                                                                                      2009
                                                                                                                                                                                                                                                                             2010
                                                                                                                                                                                                                                                                                    2011
                                                                                                                                                                                                                                                                                            2009
                                                                                                                                                                                                                                                                                                   2010
                                                                                                                                                                                                                                                                                                          2011
                                                                                                                                                                                                                                                                                                                 2009
                                                                                                                                                                                                                                                                                                                        2010
                                                                                                                                                                                                                                                                                                                               2011
                                                                                                                                                                                                                                                                                                                                      2009
                                                                                                                                                                                                                                                                                                                                             2010
                                                                                                                                                                                                                                                                                                                                                    2011
              B                 K                  NOE                  OOE                  ST                    T                    W                    V                           B                    K                    NOE                  OOE                  ST                    T                    W                    V




  LOD = 0,0002 mg/kg; LOQ = 0,001 mg/kg
                                                                                                                                                                                                              Bees: significant decline
                                                                                                                                                                                                              in percentage of positive
                                                                                                                                                                                                              samples
Legend: BG = LOQ; n.n. = not detected
Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                                                                                                                                                                                                                                                                       18
Imidacloprid – three-years-comparison of
residue results for samples of bees and bee
bread
                                                MELISSA-Vergiftungsverdacht
                               LOD           = 0,0002 mg/kg; LOQImidacloprid
                                             Rückstandsanalysen auf = 0,001 mg/kg
                                                  Matrix: Bienen und Bienenbrot

                                                                        IMI >= BG                 IMI < BG                IMI n.n.                                                            Legend:
          100%                                                                                                                                                                       0
                                                                                                                                                                                              BG = LOQ
                                                                                                                                                                                              n.n. = not detected
            90%

            80%
                                                                                                                                                                                     1
            70%
                                  14
            60%                                                                                  25
                                                                                                        33
                                                                            46 19                              63 48
            50%            1             3      1             6 30                        40                                        4                    1      1

            40%

            30%
                                                                                                                                                                                     1
            20%
                                  3
                                                                                                 3
                                                                                                                                                                                            Bees + Bee bread:
            10%
                                  3                                                              4      5
                                                                                                                                                                                            significant increase
                                                                            2                                  1      1
                                                                                   1
             0%     0      0             0      0      0      0 0           1      0      0             0      2      2      0      0      0      0 0           0      0      0             in percentage of
                    2009
                           2010
                                  2011
                                         2009
                                                2010
                                                       2011
                                                              2009
                                                                     2010
                                                                            2011
                                                                                   2009
                                                                                          2010
                                                                                                 2011
                                                                                                        2009
                                                                                                               2010
                                                                                                                      2011
                                                                                                                             2009
                                                                                                                                    2010
                                                                                                                                           2011
                                                                                                                                                  2009
                                                                                                                                                         2010
                                                                                                                                                                2011
                                                                                                                                                                       2009
                                                                                                                                                                              2010
                                                                                                                                                                                     2011
                                                                                                                                                                                            positive samples
                           B                    K                    NOE                  OOE                  ST                   T                    W                    V             from 2009 - 2011
Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                                                                                                                                19
Exposure of bee yards (%) to insecticidal seed coating
materials1 in the years 2009 – 2011 according to results of
residue analysis in bees or bee bread

                  Anteil der Bienenstände mit bzw. ohne
          Rückstandsnachweis für insektizide Saatgutbeizmittel in
     1 Clothianidin, Thiamethoxam, Imidacloprid, Fipronil               and
                          Bienen oder Bienenbrot
     Fipronil-sulfone: LOD = 0,0002 untersucht, bzw. 7 Proben nur aufmg/kg
            * 2011 wurden keine Monitoringstände mg/kg; LOQ = 0,001
                                    "sonstige Pflanzenschutzmittel" untersucht


                  100%                                  7
                          2
                   90%
                          5            27
                   80%                                                  8
                   70%                                 66
 Bienenstände




                                                                                       13                         n.u.**   Legend:
      Bee yards




                                       11
                   60%
                                                                                                                  neg.     BG = LOQ
                   50%                                                  7                                                  neg = negative
                                                                                                                  < BG
                   40%   20                            28                                                                  n.u. = not analysed
                   30%                 55                                                                         ≥ BG
                                                                                        2
                   20%
                                                       43               7                                                  *7 samples just analysed for
                   10%                                                                  4                                  „other active substances of
                    0%                                                                                                     plant protection products;
                         2009         2010            2011            2009           2010           2011*
                                                                                                                           ** in 2011, no monitoring
                         Suspected poisoning
                            Vergiftungsverdacht                             Monitoring
                                                                               Monitoring                                  beeyards were investigated;


Significant downtrend of exposed bee yards during years of study.
Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                                                                      20
Residue analysis of extracted honey
In 2009 9 samples and in 2010 62 samples were analysed for Clothianidin, Thiamethoxam,
Imidacloprid: all negative (LOD = 0,0006 mg/kg; LOQ = 0,002 mg/kg)

2011: 13 samples analysed: all were in compliance with the MRL-regulations and fully marketable
   according to the Austrian legislative framework.

  Analytes                                                        n.d.               > LOQ                    < LOQ   Samples (n=)
  *Clothianidin1                                                    11                     0                      2
  *Thiamethoxam2                                                    12                     0                      1
  *Imidacloprid1                                                    11                     1                      1        13
  *Fipronil2, *Fipronil-sulfone1                                    13                     0                      0
  Thiacloprid2                                                       5                     4                      4
  Amitraz3                                                          12                     1                      0
  Legend: n.d. = not detectable; LOQ = level of quantification; LOD = level of detection
  1 LOQ = 0,0002 mg/kg; LOD = 0,00006 mg/kg
  2 LOQ = 0,0005 mg/kg; LOD = 0,00015 mg/kg
  3 LOQ = 0,01 mg/kg

  *for these substances LOD and LOQ, respectively, were lower by a factor of 10 in 2011 compared to the years 2009 and 2010.


Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                                                 21
Wind conditions at time of maize sowing 2011
„no sowing of insecticidal coated maize and oil pumpkin seed with pneumatic seed
drills at a windspeed > 5 m/s (18 km/h)“



                                                                             Wind-peak values (wsp)

                            25

                            20
        Windspeed (m/sec)




                            15
                                                                                                                  Hours
                            10

                            5




                                                                  Day

Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                                      22
Wind conditions in April at time of maize sowing
years: 2009, 2010, 2011
„no sowing of insecticidal coated maize and oil pumpkin seed with pneumatic seed
drills at a windspeed > 5 m/s (18 km/h)“


                                                                     Neusiedl/See 2009 - 2011
                                                                          Location Neusiedl/See
                                                                     Wind-Spitzenwerte 12 Uhr
                                                                 Wind-peak values (wsp) at 12 (a.m.)
                                                                     wsp-12h-2009   wsp-12h-2010         wsp-12h-2011

                               200
                               20
        Windspeed (m/sec)
         Windgeschwindigkeit




                               150
          (Zehntelmeter/sec)




                               15

                               100
                               10


                                50
                                5

                                 0
                                     1   2   3   4   5   6   7   8   9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

                                                                                        Tag

Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                                                    23
Heubach-values of abrasion tests from
enforcement (marketing) control samples
of insecticidal coated seed batches in 2010
and 2011



  0,80
                                2010                                      2011
            Grenzwert Abrieb
            0,75 g / 100.000 permissible abrasion value: 0.75 g/100.000 kernels
             Maximum Korn
  0,70

  0,60         Average = 0.35 = 0,35 g / 100.000 Korn
                   Mittelwert g/100.000 kernels                                                 Average = 0.17 g/100.000 / 100.000 Korn
                                                                                                     Mittelwert = 0,17 g kernels

  0,50

  0,40

  0,30

  0,20

  0,10

  0,00
             1
             2
             3
             4
             5
             6
             7
             8
             9
            10
            11
            12
            13
            14
            15
            16
            17
            18
            19
            20
            21
            22
            23

                                                                                         1
                                                                                         2
                                                                                         3
                                                                                         4
                                                                                         5
                                                                                         6
                                                                                         7
                                                                                         8
                                                                                         9
                                                                                        10
                                                                                        11
                                                                                        12
                                                                                        13
                                                                                        14
                                                                                        15
                                                                                        16
                                                                                        17
                                                                                        18
                                                                                        19
                                                                                        20
                                                                                        21
                                                                                        22
                                                                                        23
                                                                                        24
                                                                                        25
Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                                                      24
Summary
• Results of the MELISSA-project give evidence that in Austria regional clustered bee
  damages had occurred in the years 2009 – 2011, which were frequently associated with
  the use of maize and oilseed pumpkin seeds coated with insecticides.

• Regulatory measures to prevent honey bee losses due to the exposure of bees to
  insecticidal seed dressing substances have significantly improved the situation. However,
  repeatedly observed incidences of honey bee mortality in defined regions suggest their
  systematic correlation with local factors contributing to increased exposure of bees.

• Seed dressing quality and seed drill equipment still need further improvement and
  sowing of insecticidal dressed seed with pneumatic seed drills has to be avoided under
  windy conditions

• Insecticidal seed dressings should be used solely based on a given risk in plant
  production with therefore reasoned indication.

• An adjustment in the approval procedure is required for risk assessment and approval of
  insecticidal substances concerning the specific characteristics and possible routes of
  exposure of bee toxic seed dressing substances.
Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                              25
For further Details, please contact


Dr. Rudolf Moosbeckhofer
AGES, Institute for Seed and Propagation Material, Phytosanitary
Service and Apiculture
Department for Apiculture and Bee Protection
rudolf.moosbeckofer@ages.at

Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP
Legislation Meeting, 27-28 Sept 2012                                                                              26

More Related Content

What's hot

Insecticides for Vegetable Production 2011
Insecticides for Vegetable Production 2011Insecticides for Vegetable Production 2011
Insecticides for Vegetable Production 2011
Ayanava Majumdar (Dr. A), Alabama Cooperative Extension System
 
Vaccines
VaccinesVaccines
Genetic engineering in baculovirus, entomopathogenic fungi and bacteria
Genetic engineering in baculovirus, entomopathogenic fungi and bacteriaGenetic engineering in baculovirus, entomopathogenic fungi and bacteria
Genetic engineering in baculovirus, entomopathogenic fungi and bacteria
Suman Sanjta
 
Micro Oraganisms used in Bio pesticide
Micro Oraganisms used in Bio pesticideMicro Oraganisms used in Bio pesticide
Micro Oraganisms used in Bio pesticide
ChamudithaBenaragama
 
Biopesticide
BiopesticideBiopesticide
Biopesticide
eswar1810
 
Pathology
PathologyPathology
10 Good Ipmphc1
10 Good Ipmphc110 Good Ipmphc1
10 Good Ipmphc1
sherylwil
 
Microbial pest control ppt
Microbial pest control ppt Microbial pest control ppt
Microbial pest control ppt
Pranjali Nene
 
insect pest management
insect pest managementinsect pest management
insect pest management
GBPUA&T, Pantnagar, (US Nagar)
 
Biopesticide-Abbas Morovvati
Biopesticide-Abbas MorovvatiBiopesticide-Abbas Morovvati
Biopesticide-Abbas Morovvati
abbasmorovvati
 
Entomopathogenic viruses
Entomopathogenic virusesEntomopathogenic viruses
Entomopathogenic viruses
Abdul Hamid Nazari
 
Aaliya Afroz
Aaliya AfrozAaliya Afroz
Aaliya Afroz
Dr. S.S. Shaw
 
Microbial insecticides
Microbial insecticidesMicrobial insecticides
Microbial insecticides
Iniya Lakshimi
 
Bio pesticides
Bio pesticidesBio pesticides
Bio pesticides
Sivasangari Shanmugam
 
Vaccination faliure
Vaccination faliureVaccination faliure
Vaccination faliure
Ossama Motawae
 
Plant Incorporated Protectants(PIPs)
Plant Incorporated Protectants(PIPs)Plant Incorporated Protectants(PIPs)
Plant Incorporated Protectants(PIPs)
Richa Khatiwada
 
Biopesticides in opm
Biopesticides in opmBiopesticides in opm
Biopesticides in opm
romila123
 
Seminar jai hind sharma
Seminar jai hind sharmaSeminar jai hind sharma
Seminar jai hind sharma
Jai Hind
 
Entomopathogenic protozoa and spiroplasma
Entomopathogenic protozoa and spiroplasmaEntomopathogenic protozoa and spiroplasma
Entomopathogenic protozoa and spiroplasma
Rajat Sharma
 
Recent advances in fungal pathogens as biopesticides
Recent advances in fungal pathogens as biopesticidesRecent advances in fungal pathogens as biopesticides
Recent advances in fungal pathogens as biopesticides
mawthammm
 

What's hot (20)

Insecticides for Vegetable Production 2011
Insecticides for Vegetable Production 2011Insecticides for Vegetable Production 2011
Insecticides for Vegetable Production 2011
 
Vaccines
VaccinesVaccines
Vaccines
 
Genetic engineering in baculovirus, entomopathogenic fungi and bacteria
Genetic engineering in baculovirus, entomopathogenic fungi and bacteriaGenetic engineering in baculovirus, entomopathogenic fungi and bacteria
Genetic engineering in baculovirus, entomopathogenic fungi and bacteria
 
Micro Oraganisms used in Bio pesticide
Micro Oraganisms used in Bio pesticideMicro Oraganisms used in Bio pesticide
Micro Oraganisms used in Bio pesticide
 
Biopesticide
BiopesticideBiopesticide
Biopesticide
 
Pathology
PathologyPathology
Pathology
 
10 Good Ipmphc1
10 Good Ipmphc110 Good Ipmphc1
10 Good Ipmphc1
 
Microbial pest control ppt
Microbial pest control ppt Microbial pest control ppt
Microbial pest control ppt
 
insect pest management
insect pest managementinsect pest management
insect pest management
 
Biopesticide-Abbas Morovvati
Biopesticide-Abbas MorovvatiBiopesticide-Abbas Morovvati
Biopesticide-Abbas Morovvati
 
Entomopathogenic viruses
Entomopathogenic virusesEntomopathogenic viruses
Entomopathogenic viruses
 
Aaliya Afroz
Aaliya AfrozAaliya Afroz
Aaliya Afroz
 
Microbial insecticides
Microbial insecticidesMicrobial insecticides
Microbial insecticides
 
Bio pesticides
Bio pesticidesBio pesticides
Bio pesticides
 
Vaccination faliure
Vaccination faliureVaccination faliure
Vaccination faliure
 
Plant Incorporated Protectants(PIPs)
Plant Incorporated Protectants(PIPs)Plant Incorporated Protectants(PIPs)
Plant Incorporated Protectants(PIPs)
 
Biopesticides in opm
Biopesticides in opmBiopesticides in opm
Biopesticides in opm
 
Seminar jai hind sharma
Seminar jai hind sharmaSeminar jai hind sharma
Seminar jai hind sharma
 
Entomopathogenic protozoa and spiroplasma
Entomopathogenic protozoa and spiroplasmaEntomopathogenic protozoa and spiroplasma
Entomopathogenic protozoa and spiroplasma
 
Recent advances in fungal pathogens as biopesticides
Recent advances in fungal pathogens as biopesticidesRecent advances in fungal pathogens as biopesticides
Recent advances in fungal pathogens as biopesticides
 

Viewers also liked

„Lebensmittelsicherheit in der EU: Verantwortung des Herstellers oder der Beh...
„Lebensmittelsicherheit in der EU: Verantwortung des Herstellers oder der Beh...„Lebensmittelsicherheit in der EU: Verantwortung des Herstellers oder der Beh...
„Lebensmittelsicherheit in der EU: Verantwortung des Herstellers oder der Beh...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
AGES presentation of the Division of Public Health at Meeting with ECDC
AGES presentation of the Division of Public Health at Meeting with ECDCAGES presentation of the Division of Public Health at Meeting with ECDC
AGES presentation of the Division of Public Health at Meeting with ECDC
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
Peter Hufnagl zur Public Health-Bedeutung von Neobiota: "Gelsenmonitoring in ...
Peter Hufnagl zur Public Health-Bedeutung von Neobiota: "Gelsenmonitoring in ...Peter Hufnagl zur Public Health-Bedeutung von Neobiota: "Gelsenmonitoring in ...
Peter Hufnagl zur Public Health-Bedeutung von Neobiota: "Gelsenmonitoring in ...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
„Listerien in Lebensmitteln in Österreich“ – Ariane PIETZKA (AGES Graz)
„Listerien in Lebensmitteln in Österreich“ – Ariane PIETZKA (AGES Graz)„Listerien in Lebensmitteln in Österreich“ – Ariane PIETZKA (AGES Graz)
„Listerien in Lebensmitteln in Österreich“ – Ariane PIETZKA (AGES Graz)
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
"Präsentation des Resistenberichts AURES 2012", Petra Apfalter (NRZ Linz) und...
"Präsentation des Resistenberichts AURES 2012", Petra Apfalter (NRZ Linz) und..."Präsentation des Resistenberichts AURES 2012", Petra Apfalter (NRZ Linz) und...
"Präsentation des Resistenberichts AURES 2012", Petra Apfalter (NRZ Linz) und...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
„Listerien in Fischprodukten“ - Marija Zunabovic & Konrad J. Domig (BoKu, Wien)
„Listerien in Fischprodukten“ - Marija Zunabovic & Konrad J. Domig (BoKu, Wien)„Listerien in Fischprodukten“ - Marija Zunabovic & Konrad J. Domig (BoKu, Wien)
„Listerien in Fischprodukten“ - Marija Zunabovic & Konrad J. Domig (BoKu, Wien)
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
Josef Mayr zu Zwischenergebnissen des Projektes Bioenergy-Silphium und Beflug...
Josef Mayr zu Zwischenergebnissen des Projektes Bioenergy-Silphium und Beflug...Josef Mayr zu Zwischenergebnissen des Projektes Bioenergy-Silphium und Beflug...
Josef Mayr zu Zwischenergebnissen des Projektes Bioenergy-Silphium und Beflug...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
AGES Austrian Agency for Health and Food Safety - RESEARCH FOCUS presentation...
AGES Austrian Agency for Health and Food Safety - RESEARCH FOCUS presentation...AGES Austrian Agency for Health and Food Safety - RESEARCH FOCUS presentation...
AGES Austrian Agency for Health and Food Safety - RESEARCH FOCUS presentation...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
Rudolf Moosbeckhofer zu Neobiota: "Bienengesundheit im Zusammenhang mit einge...
Rudolf Moosbeckhofer zu Neobiota: "Bienengesundheit im Zusammenhang mit einge...Rudolf Moosbeckhofer zu Neobiota: "Bienengesundheit im Zusammenhang mit einge...
Rudolf Moosbeckhofer zu Neobiota: "Bienengesundheit im Zusammenhang mit einge...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
Vom Input zum Impact: Entwicklung der AGES 2002 bis 2015
Vom Input zum Impact: Entwicklung der AGES 2002 bis 2015 Vom Input zum Impact: Entwicklung der AGES 2002 bis 2015
Vom Input zum Impact: Entwicklung der AGES 2002 bis 2015
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
Swen Follak zu gesundheitlicher Bedeutung allergener Neobiota: "Ausbreitungsd...
Swen Follak zu gesundheitlicher Bedeutung allergener Neobiota: "Ausbreitungsd...Swen Follak zu gesundheitlicher Bedeutung allergener Neobiota: "Ausbreitungsd...
Swen Follak zu gesundheitlicher Bedeutung allergener Neobiota: "Ausbreitungsd...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
Kleiner Bienenstockkäfer in Italien nachgewiesen
Kleiner Bienenstockkäfer in Italien nachgewiesenKleiner Bienenstockkäfer in Italien nachgewiesen
Kleiner Bienenstockkäfer in Italien nachgewiesen
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
"Vector-borne diseases in Österreich" - Friedrich Schmoll (AGES, Tiergesundhe...
"Vector-borne diseases in Österreich" - Friedrich Schmoll (AGES, Tiergesundhe..."Vector-borne diseases in Österreich" - Friedrich Schmoll (AGES, Tiergesundhe...
"Vector-borne diseases in Österreich" - Friedrich Schmoll (AGES, Tiergesundhe...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
"Verkaufsdaten von Veterinärantibiotika in 25 europäischen Staaten" - Klemens...
"Verkaufsdaten von Veterinärantibiotika in 25 europäischen Staaten" - Klemens..."Verkaufsdaten von Veterinärantibiotika in 25 europäischen Staaten" - Klemens...
"Verkaufsdaten von Veterinärantibiotika in 25 europäischen Staaten" - Klemens...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 

Viewers also liked (15)

„Lebensmittelsicherheit in der EU: Verantwortung des Herstellers oder der Beh...
„Lebensmittelsicherheit in der EU: Verantwortung des Herstellers oder der Beh...„Lebensmittelsicherheit in der EU: Verantwortung des Herstellers oder der Beh...
„Lebensmittelsicherheit in der EU: Verantwortung des Herstellers oder der Beh...
 
AGES presentation of the Division of Public Health at Meeting with ECDC
AGES presentation of the Division of Public Health at Meeting with ECDCAGES presentation of the Division of Public Health at Meeting with ECDC
AGES presentation of the Division of Public Health at Meeting with ECDC
 
Peter Hufnagl zur Public Health-Bedeutung von Neobiota: "Gelsenmonitoring in ...
Peter Hufnagl zur Public Health-Bedeutung von Neobiota: "Gelsenmonitoring in ...Peter Hufnagl zur Public Health-Bedeutung von Neobiota: "Gelsenmonitoring in ...
Peter Hufnagl zur Public Health-Bedeutung von Neobiota: "Gelsenmonitoring in ...
 
„Listerien in Lebensmitteln in Österreich“ – Ariane PIETZKA (AGES Graz)
„Listerien in Lebensmitteln in Österreich“ – Ariane PIETZKA (AGES Graz)„Listerien in Lebensmitteln in Österreich“ – Ariane PIETZKA (AGES Graz)
„Listerien in Lebensmitteln in Österreich“ – Ariane PIETZKA (AGES Graz)
 
"Präsentation des Resistenberichts AURES 2012", Petra Apfalter (NRZ Linz) und...
"Präsentation des Resistenberichts AURES 2012", Petra Apfalter (NRZ Linz) und..."Präsentation des Resistenberichts AURES 2012", Petra Apfalter (NRZ Linz) und...
"Präsentation des Resistenberichts AURES 2012", Petra Apfalter (NRZ Linz) und...
 
„Listerien in Fischprodukten“ - Marija Zunabovic & Konrad J. Domig (BoKu, Wien)
„Listerien in Fischprodukten“ - Marija Zunabovic & Konrad J. Domig (BoKu, Wien)„Listerien in Fischprodukten“ - Marija Zunabovic & Konrad J. Domig (BoKu, Wien)
„Listerien in Fischprodukten“ - Marija Zunabovic & Konrad J. Domig (BoKu, Wien)
 
Josef Mayr zu Zwischenergebnissen des Projektes Bioenergy-Silphium und Beflug...
Josef Mayr zu Zwischenergebnissen des Projektes Bioenergy-Silphium und Beflug...Josef Mayr zu Zwischenergebnissen des Projektes Bioenergy-Silphium und Beflug...
Josef Mayr zu Zwischenergebnissen des Projektes Bioenergy-Silphium und Beflug...
 
AGES Austrian Agency for Health and Food Safety - RESEARCH FOCUS presentation...
AGES Austrian Agency for Health and Food Safety - RESEARCH FOCUS presentation...AGES Austrian Agency for Health and Food Safety - RESEARCH FOCUS presentation...
AGES Austrian Agency for Health and Food Safety - RESEARCH FOCUS presentation...
 
Rudolf Moosbeckhofer zu Neobiota: "Bienengesundheit im Zusammenhang mit einge...
Rudolf Moosbeckhofer zu Neobiota: "Bienengesundheit im Zusammenhang mit einge...Rudolf Moosbeckhofer zu Neobiota: "Bienengesundheit im Zusammenhang mit einge...
Rudolf Moosbeckhofer zu Neobiota: "Bienengesundheit im Zusammenhang mit einge...
 
Vom Input zum Impact: Entwicklung der AGES 2002 bis 2015
Vom Input zum Impact: Entwicklung der AGES 2002 bis 2015 Vom Input zum Impact: Entwicklung der AGES 2002 bis 2015
Vom Input zum Impact: Entwicklung der AGES 2002 bis 2015
 
Swen Follak zu gesundheitlicher Bedeutung allergener Neobiota: "Ausbreitungsd...
Swen Follak zu gesundheitlicher Bedeutung allergener Neobiota: "Ausbreitungsd...Swen Follak zu gesundheitlicher Bedeutung allergener Neobiota: "Ausbreitungsd...
Swen Follak zu gesundheitlicher Bedeutung allergener Neobiota: "Ausbreitungsd...
 
Kleiner Bienenstockkäfer in Italien nachgewiesen
Kleiner Bienenstockkäfer in Italien nachgewiesenKleiner Bienenstockkäfer in Italien nachgewiesen
Kleiner Bienenstockkäfer in Italien nachgewiesen
 
Vortrag: "Pflanzenschutzmittel - Fakten statt Hysterie", Dr. Albert Bergmann ...
Vortrag: "Pflanzenschutzmittel - Fakten statt Hysterie", Dr. Albert Bergmann ...Vortrag: "Pflanzenschutzmittel - Fakten statt Hysterie", Dr. Albert Bergmann ...
Vortrag: "Pflanzenschutzmittel - Fakten statt Hysterie", Dr. Albert Bergmann ...
 
"Vector-borne diseases in Österreich" - Friedrich Schmoll (AGES, Tiergesundhe...
"Vector-borne diseases in Österreich" - Friedrich Schmoll (AGES, Tiergesundhe..."Vector-borne diseases in Österreich" - Friedrich Schmoll (AGES, Tiergesundhe...
"Vector-borne diseases in Österreich" - Friedrich Schmoll (AGES, Tiergesundhe...
 
"Verkaufsdaten von Veterinärantibiotika in 25 europäischen Staaten" - Klemens...
"Verkaufsdaten von Veterinärantibiotika in 25 europäischen Staaten" - Klemens..."Verkaufsdaten von Veterinärantibiotika in 25 europäischen Staaten" - Klemens...
"Verkaufsdaten von Veterinärantibiotika in 25 europäischen Staaten" - Klemens...
 

Similar to MELISSA: Investigations in the incidence of bee losses in corn and oilseed rape growing areas of Austria and possible correlations with bee diseases and the use of insecticidal plant protection products

Lambert, P., 2012.
Lambert, P., 2012. Lambert, P., 2012.
Lambert, P., 2012.
fruitbreedomics
 
Franz Streissl - Bee risk assessment
Franz Streissl - Bee risk assessmentFranz Streissl - Bee risk assessment
Franz Streissl - Bee risk assessment
cropprotection
 
peerj-1413
peerj-1413peerj-1413
peerj-1413
Alex Dutka
 
Presentation 2015 eng s
Presentation 2015 eng sPresentation 2015 eng s
Presentation 2015 eng s
agrobiotech
 
Mason R et al 2013 Journal of Environmental Immunology and Toxicology
Mason R et al 2013 Journal of Environmental Immunology and ToxicologyMason R et al 2013 Journal of Environmental Immunology and Toxicology
Mason R et al 2013 Journal of Environmental Immunology and Toxicology
Henk Tennekes
 
Conférence Apimondia
Conférence ApimondiaConférence Apimondia
Conférence Apimondia
MacWlem S
 
Neonicotinoids and Their Current Status
Neonicotinoids and Their Current StatusNeonicotinoids and Their Current Status
Neonicotinoids and Their Current Status
SohailMaqsood786
 
Piotr Medrzycki Apimondia 2009
Piotr Medrzycki   Apimondia 2009Piotr Medrzycki   Apimondia 2009
Piotr Medrzycki Apimondia 2009
MacWlem S
 
Aflatoxins in Grains and Food Safety, Guantai, S.M., ACDI/VOCA Kenya
Aflatoxins in Grains and Food Safety, Guantai, S.M., ACDI/VOCA KenyaAflatoxins in Grains and Food Safety, Guantai, S.M., ACDI/VOCA Kenya
Aflatoxins in Grains and Food Safety, Guantai, S.M., ACDI/VOCA Kenya
pchenevixtrench
 
Neonicotinoids: The Science and Regulatory Complexity
Neonicotinoids: The Science and Regulatory ComplexityNeonicotinoids: The Science and Regulatory Complexity
Neonicotinoids: The Science and Regulatory Complexity
Covance
 
Control of wax moth, galleria mellonella l
Control of wax moth, galleria mellonella lControl of wax moth, galleria mellonella l
Control of wax moth, galleria mellonella l
Alexander Decker
 
Mycotoxin effect on seed quality
Mycotoxin effect on seed quality Mycotoxin effect on seed quality
Mycotoxin effect on seed quality
Sanggonda
 
Biosafety and Biotechnology in Africa: Eswatini's Experience
Biosafety and Biotechnology in Africa: Eswatini's ExperienceBiosafety and Biotechnology in Africa: Eswatini's Experience
Biosafety and Biotechnology in Africa: Eswatini's Experience
ESD UNU-IAS
 
Pathogenic bacteria associated with different categories of food
Pathogenic bacteria associated with different categories of foodPathogenic bacteria associated with different categories of food
Pathogenic bacteria associated with different categories of food
Tatjana Marcenkova
 
Prof Dr mohammed elhady Mycotoxins and its managment
Prof Dr mohammed elhady Mycotoxins and its managmentProf Dr mohammed elhady Mycotoxins and its managment
Prof Dr mohammed elhady Mycotoxins and its managment
AdhamOmarSallam
 
Outlooks on Pest Management - June 2015 Al-Amidi article
Outlooks on Pest Management - June 2015 Al-Amidi  articleOutlooks on Pest Management - June 2015 Al-Amidi  article
Outlooks on Pest Management - June 2015 Al-Amidi article
Dr. Abdul Hamid Al Amidi
 
Environment & Food Safety
Environment & Food SafetyEnvironment & Food Safety
Environment & Food Safety
ddsskk
 
Foot and mouth disease preventive and epidemiological aspects
Foot and mouth disease preventive and epidemiological aspectsFoot and mouth disease preventive and epidemiological aspects
Foot and mouth disease preventive and epidemiological aspects
Bhoj Raj Singh
 
Response of potential stored grain insect pests to bfl 225 multi attractant l...
Response of potential stored grain insect pests to bfl 225 multi attractant l...Response of potential stored grain insect pests to bfl 225 multi attractant l...
Response of potential stored grain insect pests to bfl 225 multi attractant l...
Alexander Decker
 
Global adventitious agent regulation of raw materials ibc sept 2010 final ver...
Global adventitious agent regulation of raw materials ibc sept 2010 final ver...Global adventitious agent regulation of raw materials ibc sept 2010 final ver...
Global adventitious agent regulation of raw materials ibc sept 2010 final ver...
chalverson
 

Similar to MELISSA: Investigations in the incidence of bee losses in corn and oilseed rape growing areas of Austria and possible correlations with bee diseases and the use of insecticidal plant protection products (20)

Lambert, P., 2012.
Lambert, P., 2012. Lambert, P., 2012.
Lambert, P., 2012.
 
Franz Streissl - Bee risk assessment
Franz Streissl - Bee risk assessmentFranz Streissl - Bee risk assessment
Franz Streissl - Bee risk assessment
 
peerj-1413
peerj-1413peerj-1413
peerj-1413
 
Presentation 2015 eng s
Presentation 2015 eng sPresentation 2015 eng s
Presentation 2015 eng s
 
Mason R et al 2013 Journal of Environmental Immunology and Toxicology
Mason R et al 2013 Journal of Environmental Immunology and ToxicologyMason R et al 2013 Journal of Environmental Immunology and Toxicology
Mason R et al 2013 Journal of Environmental Immunology and Toxicology
 
Conférence Apimondia
Conférence ApimondiaConférence Apimondia
Conférence Apimondia
 
Neonicotinoids and Their Current Status
Neonicotinoids and Their Current StatusNeonicotinoids and Their Current Status
Neonicotinoids and Their Current Status
 
Piotr Medrzycki Apimondia 2009
Piotr Medrzycki   Apimondia 2009Piotr Medrzycki   Apimondia 2009
Piotr Medrzycki Apimondia 2009
 
Aflatoxins in Grains and Food Safety, Guantai, S.M., ACDI/VOCA Kenya
Aflatoxins in Grains and Food Safety, Guantai, S.M., ACDI/VOCA KenyaAflatoxins in Grains and Food Safety, Guantai, S.M., ACDI/VOCA Kenya
Aflatoxins in Grains and Food Safety, Guantai, S.M., ACDI/VOCA Kenya
 
Neonicotinoids: The Science and Regulatory Complexity
Neonicotinoids: The Science and Regulatory ComplexityNeonicotinoids: The Science and Regulatory Complexity
Neonicotinoids: The Science and Regulatory Complexity
 
Control of wax moth, galleria mellonella l
Control of wax moth, galleria mellonella lControl of wax moth, galleria mellonella l
Control of wax moth, galleria mellonella l
 
Mycotoxin effect on seed quality
Mycotoxin effect on seed quality Mycotoxin effect on seed quality
Mycotoxin effect on seed quality
 
Biosafety and Biotechnology in Africa: Eswatini's Experience
Biosafety and Biotechnology in Africa: Eswatini's ExperienceBiosafety and Biotechnology in Africa: Eswatini's Experience
Biosafety and Biotechnology in Africa: Eswatini's Experience
 
Pathogenic bacteria associated with different categories of food
Pathogenic bacteria associated with different categories of foodPathogenic bacteria associated with different categories of food
Pathogenic bacteria associated with different categories of food
 
Prof Dr mohammed elhady Mycotoxins and its managment
Prof Dr mohammed elhady Mycotoxins and its managmentProf Dr mohammed elhady Mycotoxins and its managment
Prof Dr mohammed elhady Mycotoxins and its managment
 
Outlooks on Pest Management - June 2015 Al-Amidi article
Outlooks on Pest Management - June 2015 Al-Amidi  articleOutlooks on Pest Management - June 2015 Al-Amidi  article
Outlooks on Pest Management - June 2015 Al-Amidi article
 
Environment & Food Safety
Environment & Food SafetyEnvironment & Food Safety
Environment & Food Safety
 
Foot and mouth disease preventive and epidemiological aspects
Foot and mouth disease preventive and epidemiological aspectsFoot and mouth disease preventive and epidemiological aspects
Foot and mouth disease preventive and epidemiological aspects
 
Response of potential stored grain insect pests to bfl 225 multi attractant l...
Response of potential stored grain insect pests to bfl 225 multi attractant l...Response of potential stored grain insect pests to bfl 225 multi attractant l...
Response of potential stored grain insect pests to bfl 225 multi attractant l...
 
Global adventitious agent regulation of raw materials ibc sept 2010 final ver...
Global adventitious agent regulation of raw materials ibc sept 2010 final ver...Global adventitious agent regulation of raw materials ibc sept 2010 final ver...
Global adventitious agent regulation of raw materials ibc sept 2010 final ver...
 

More from AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit

AGES West Nil Folder
AGES West Nil FolderAGES West Nil Folder
"Health claims aus Sicht der amtlichen Lebensmittelkontrolle" - Markus Zsivko...
"Health claims aus Sicht der amtlichen Lebensmittelkontrolle" - Markus Zsivko..."Health claims aus Sicht der amtlichen Lebensmittelkontrolle" - Markus Zsivko...
"Health claims aus Sicht der amtlichen Lebensmittelkontrolle" - Markus Zsivko...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
„Listeriose bei Säugetieren in Österreich“ - Zoltan Bago (AGES, IVET)
„Listeriose bei Säugetieren in Österreich“ - Zoltan Bago (AGES, IVET) „Listeriose bei Säugetieren in Österreich“ - Zoltan Bago (AGES, IVET)
„Listeriose bei Säugetieren in Österreich“ - Zoltan Bago (AGES, IVET)
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
„Listeriose beim Menschen in Österreich“ - Franz Allerberger (AGES)
„Listeriose beim Menschen in Österreich“ - Franz Allerberger (AGES)„Listeriose beim Menschen in Österreich“ - Franz Allerberger (AGES)
„Listeriose beim Menschen in Österreich“ - Franz Allerberger (AGES)
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
„Vom Corynebacterium infantisepticum zu Listeria monocytogenes - In Memoriam ...
„Vom Corynebacterium infantisepticum zu Listeria monocytogenes - In Memoriam ...„Vom Corynebacterium infantisepticum zu Listeria monocytogenes - In Memoriam ...
„Vom Corynebacterium infantisepticum zu Listeria monocytogenes - In Memoriam ...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
"The new EURL Guidelines on sampling the food processing area and equipment f...
"The new EURL Guidelines on sampling the food processing area and equipment f..."The new EURL Guidelines on sampling the food processing area and equipment f...
"The new EURL Guidelines on sampling the food processing area and equipment f...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
"Overview of the European Union reference laboratory (eu rl) for Listeria mon...
"Overview of the European Union reference laboratory (eu rl) for Listeria mon..."Overview of the European Union reference laboratory (eu rl) for Listeria mon...
"Overview of the European Union reference laboratory (eu rl) for Listeria mon...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
"Kupfer im Pflanzenschutz - Ersatzstrategien von Kupfer im Weinbau" - Helga R...
"Kupfer im Pflanzenschutz - Ersatzstrategien von Kupfer im Weinbau" - Helga R..."Kupfer im Pflanzenschutz - Ersatzstrategien von Kupfer im Weinbau" - Helga R...
"Kupfer im Pflanzenschutz - Ersatzstrategien von Kupfer im Weinbau" - Helga R...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
"Medizinprodukte", Dr. Reinhard Berger, Institut Inspektionen, Medizinprodukt...
"Medizinprodukte", Dr. Reinhard Berger, Institut Inspektionen, Medizinprodukt..."Medizinprodukte", Dr. Reinhard Berger, Institut Inspektionen, Medizinprodukt...
"Medizinprodukte", Dr. Reinhard Berger, Institut Inspektionen, Medizinprodukt...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
"Risiken von ausgewählten Mineralstoff- und Vitaminsupplementen" - Cem Ekmekc...
"Risiken von ausgewählten Mineralstoff- und Vitaminsupplementen" - Cem Ekmekc..."Risiken von ausgewählten Mineralstoff- und Vitaminsupplementen" - Cem Ekmekc...
"Risiken von ausgewählten Mineralstoff- und Vitaminsupplementen" - Cem Ekmekc...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
"Vitamin D - Nahrungsergänzung für alle? Stellungnahme aus Sicht der Risikobe...
"Vitamin D - Nahrungsergänzung für alle? Stellungnahme aus Sicht der Risikobe..."Vitamin D - Nahrungsergänzung für alle? Stellungnahme aus Sicht der Risikobe...
"Vitamin D - Nahrungsergänzung für alle? Stellungnahme aus Sicht der Risikobe...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
"Risiken pflanzlicher Stoffe – NEM ohne Risiken ?" - Prof. Dr. Dr. Alfonso La...
"Risiken pflanzlicher Stoffe – NEM ohne Risiken ?" - Prof. Dr. Dr. Alfonso La..."Risiken pflanzlicher Stoffe – NEM ohne Risiken ?" - Prof. Dr. Dr. Alfonso La...
"Risiken pflanzlicher Stoffe – NEM ohne Risiken ?" - Prof. Dr. Dr. Alfonso La...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
"Abgrenzung von Arzneimitteln zu Nahrungsergänzungsmitteln" - Dr. Michael Beh...
"Abgrenzung von Arzneimitteln zu Nahrungsergänzungsmitteln" - Dr. Michael Beh..."Abgrenzung von Arzneimitteln zu Nahrungsergänzungsmitteln" - Dr. Michael Beh...
"Abgrenzung von Arzneimitteln zu Nahrungsergänzungsmitteln" - Dr. Michael Beh...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
"Möglichkeiten und Grenzen der amtlichen Lebensmittelkontrolle bei Nahrungser...
"Möglichkeiten und Grenzen der amtlichen Lebensmittelkontrolle bei Nahrungser..."Möglichkeiten und Grenzen der amtlichen Lebensmittelkontrolle bei Nahrungser...
"Möglichkeiten und Grenzen der amtlichen Lebensmittelkontrolle bei Nahrungser...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 
"Methoden und Herausforderungen der Risikokommunikation bei der Thematik Nahr...
"Methoden und Herausforderungen der Risikokommunikation bei der Thematik Nahr..."Methoden und Herausforderungen der Risikokommunikation bei der Thematik Nahr...
"Methoden und Herausforderungen der Risikokommunikation bei der Thematik Nahr...
AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit
 

More from AGES - Österreichische Agentur für Gesundheit und Ernährungssicherheit (15)

AGES West Nil Folder
AGES West Nil FolderAGES West Nil Folder
AGES West Nil Folder
 
"Health claims aus Sicht der amtlichen Lebensmittelkontrolle" - Markus Zsivko...
"Health claims aus Sicht der amtlichen Lebensmittelkontrolle" - Markus Zsivko..."Health claims aus Sicht der amtlichen Lebensmittelkontrolle" - Markus Zsivko...
"Health claims aus Sicht der amtlichen Lebensmittelkontrolle" - Markus Zsivko...
 
„Listeriose bei Säugetieren in Österreich“ - Zoltan Bago (AGES, IVET)
„Listeriose bei Säugetieren in Österreich“ - Zoltan Bago (AGES, IVET) „Listeriose bei Säugetieren in Österreich“ - Zoltan Bago (AGES, IVET)
„Listeriose bei Säugetieren in Österreich“ - Zoltan Bago (AGES, IVET)
 
„Listeriose beim Menschen in Österreich“ - Franz Allerberger (AGES)
„Listeriose beim Menschen in Österreich“ - Franz Allerberger (AGES)„Listeriose beim Menschen in Österreich“ - Franz Allerberger (AGES)
„Listeriose beim Menschen in Österreich“ - Franz Allerberger (AGES)
 
„Vom Corynebacterium infantisepticum zu Listeria monocytogenes - In Memoriam ...
„Vom Corynebacterium infantisepticum zu Listeria monocytogenes - In Memoriam ...„Vom Corynebacterium infantisepticum zu Listeria monocytogenes - In Memoriam ...
„Vom Corynebacterium infantisepticum zu Listeria monocytogenes - In Memoriam ...
 
"The new EURL Guidelines on sampling the food processing area and equipment f...
"The new EURL Guidelines on sampling the food processing area and equipment f..."The new EURL Guidelines on sampling the food processing area and equipment f...
"The new EURL Guidelines on sampling the food processing area and equipment f...
 
"Overview of the European Union reference laboratory (eu rl) for Listeria mon...
"Overview of the European Union reference laboratory (eu rl) for Listeria mon..."Overview of the European Union reference laboratory (eu rl) for Listeria mon...
"Overview of the European Union reference laboratory (eu rl) for Listeria mon...
 
"Kupfer im Pflanzenschutz - Ersatzstrategien von Kupfer im Weinbau" - Helga R...
"Kupfer im Pflanzenschutz - Ersatzstrategien von Kupfer im Weinbau" - Helga R..."Kupfer im Pflanzenschutz - Ersatzstrategien von Kupfer im Weinbau" - Helga R...
"Kupfer im Pflanzenschutz - Ersatzstrategien von Kupfer im Weinbau" - Helga R...
 
"Medizinprodukte", Dr. Reinhard Berger, Institut Inspektionen, Medizinprodukt...
"Medizinprodukte", Dr. Reinhard Berger, Institut Inspektionen, Medizinprodukt..."Medizinprodukte", Dr. Reinhard Berger, Institut Inspektionen, Medizinprodukt...
"Medizinprodukte", Dr. Reinhard Berger, Institut Inspektionen, Medizinprodukt...
 
"Risiken von ausgewählten Mineralstoff- und Vitaminsupplementen" - Cem Ekmekc...
"Risiken von ausgewählten Mineralstoff- und Vitaminsupplementen" - Cem Ekmekc..."Risiken von ausgewählten Mineralstoff- und Vitaminsupplementen" - Cem Ekmekc...
"Risiken von ausgewählten Mineralstoff- und Vitaminsupplementen" - Cem Ekmekc...
 
"Vitamin D - Nahrungsergänzung für alle? Stellungnahme aus Sicht der Risikobe...
"Vitamin D - Nahrungsergänzung für alle? Stellungnahme aus Sicht der Risikobe..."Vitamin D - Nahrungsergänzung für alle? Stellungnahme aus Sicht der Risikobe...
"Vitamin D - Nahrungsergänzung für alle? Stellungnahme aus Sicht der Risikobe...
 
"Risiken pflanzlicher Stoffe – NEM ohne Risiken ?" - Prof. Dr. Dr. Alfonso La...
"Risiken pflanzlicher Stoffe – NEM ohne Risiken ?" - Prof. Dr. Dr. Alfonso La..."Risiken pflanzlicher Stoffe – NEM ohne Risiken ?" - Prof. Dr. Dr. Alfonso La...
"Risiken pflanzlicher Stoffe – NEM ohne Risiken ?" - Prof. Dr. Dr. Alfonso La...
 
"Abgrenzung von Arzneimitteln zu Nahrungsergänzungsmitteln" - Dr. Michael Beh...
"Abgrenzung von Arzneimitteln zu Nahrungsergänzungsmitteln" - Dr. Michael Beh..."Abgrenzung von Arzneimitteln zu Nahrungsergänzungsmitteln" - Dr. Michael Beh...
"Abgrenzung von Arzneimitteln zu Nahrungsergänzungsmitteln" - Dr. Michael Beh...
 
"Möglichkeiten und Grenzen der amtlichen Lebensmittelkontrolle bei Nahrungser...
"Möglichkeiten und Grenzen der amtlichen Lebensmittelkontrolle bei Nahrungser..."Möglichkeiten und Grenzen der amtlichen Lebensmittelkontrolle bei Nahrungser...
"Möglichkeiten und Grenzen der amtlichen Lebensmittelkontrolle bei Nahrungser...
 
"Methoden und Herausforderungen der Risikokommunikation bei der Thematik Nahr...
"Methoden und Herausforderungen der Risikokommunikation bei der Thematik Nahr..."Methoden und Herausforderungen der Risikokommunikation bei der Thematik Nahr...
"Methoden und Herausforderungen der Risikokommunikation bei der Thematik Nahr...
 

MELISSA: Investigations in the incidence of bee losses in corn and oilseed rape growing areas of Austria and possible correlations with bee diseases and the use of insecticidal plant protection products

  • 1. Investigations on the incidence of bee losses in corn and oilseed rape growing areas of Austria and possible correlations with bee diseases and the use of insecticidal plant protection products (Acronym: MELISSA) Order for project: Federal Ministry of Agriculture, Forestry, the Environment and Water Management (BMLFUW) Funding: BMLFUW, Federal provinces of Austria, AGES, Consortium of seed and plant protection companies Duration: 1.3.2009 – 31.1.2012 Details and full report: www.dafne.at Project Nr. 100472 Project manager: DI Leopold Girsch Involved AGES-Divisions and Departments: Division for Food Security: Inst. for Seed and Propagating Material, Phytosanitary Service and Apiculture; Inst. for Sustainable Plant Production; Inst. for Plant Protection Products; Division for Food Safety; Department Data, Statistics & Risk Assessment www.ages.at Austrian Agency for Health and Food Safety (AGES)
  • 2. MELISSA Objectives • documentation of incidences of honey bee losses in production areas of maize and oilseed rape • analysis of possible causes (honey bee pathogens and parasites, plant protection products) • evaluation of results with respect to measures taken to prevent honey bee losses • development of decision guidances for authorities, beekeepers and farmers for the implementation of measures to prevent honey bee losses by pathogens, parasites and plant protection products Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: www.ages.at 2 Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012
  • 3. Studies conducted • diagnosis for pathogens and parasites in honey bees Varroa destructor, Nosema spp., 7 honey bee viruses • analysis for residues - insecticidal seed coating products - other active substances of plant protection products sampled matrices: bees, beebread, extracted honey plants seed Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: www.ages.at 3 Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012
  • 4. Symptoms in honey bees and bee colonies - increased numbers of dead bees inside and outside the hives - flightless bees with symptoms of trembling, cramping, disorientation, paralysis, abnormal wing movements, hyperexcitation, disordered locomotory activity o at time of maize sowing (simultaneously or from next day onwards) o ca. 10 – 14 days after beginning of maize sowing - flightless crawling bees or groups of bees on the soil or in the grass in front of beehives o from the beginning of maize sowing o in many cases also considerably later in the season (May until beginning of June) - intensity and duration of symptoms o one to several days – in some cases considerably longer o in some particular cases massive damages occured when bees showed a multiple contamination by insecticidal seed coating substances and other insecticides Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: www.ages.at 4 Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012
  • 5. Observed symptoms in the apiaries (1) • increased bee mortality Residue analysis in bees: Residue analysis in bees: *Clothianidin 0.0021 mg/kg; *Clothianidin 0,0033 mg/kg; *Thiamethoxam 0,0088 mg/kg *Imidacloprid 0,0273 mg/kg *LOD = 0,0002 mg/kg; *LOQ = 0,001 mg/kg Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 5
  • 6. Observed symptoms in the apiaries (2) • flightless crawling bees from the beginning of maize sowing and in many cases also considerably later in the season Residue analysis: Residue analysis: bees: negative; bees: negative bee bread positive: *Imidacloprid < LOQ **Thiacloprid < LOQ *LOD = 0,0002 mg/kg; *LOQ = 0,001 mg/kg; ** LOQ: 0,01 mg/kg Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 6
  • 7. Observed symptoms in the apiaries (3) • large quantities of dead bees in cases of multiple contamination by insecticidal seed coating substances and other insecticides Insecticides detected in dead bees by residue analysis: *Clothianidin, *Fipronil, *Fipronil-sulfone, **Chlorpyrifos *LOD = 0,0002 mg/kg; *LOQ = 0,001 mg/kg; ** LOQ: 0,01 mg/kg Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 7
  • 8. Bee yards with suspected bee poisonig – results of residue analysis year 2009 Insecticidal seed coating materials: Clothianidin, Thiamethoxam, Imidacloprid, Fipronil and Fipronil-sulfone < LOQ ≥ LOQ negative Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 8
  • 9. Bee yards with suspected bee poisonig – results of residue analysis year 2010 Insecticidal seed coating materials: Clothianidin, Thiamethoxam, Imidacloprid, Fipronil and Fipronil-sulfone < LOQ ≥ LOQ negative Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 9
  • 10. Bee yards with suspected bee poisonig – results of residue analysis year 2011 Insecticidal seed coating materials: Clothianidin, Thiamethoxam, Imidacloprid, Fipronil and Fipronil-sulfone < LOQ ≥ LOQ negative Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 10
  • 11. Results of residue analysis for „other plant protection substances“ Bees and Beebread – Several different active substances were detected – insecticides – herbicides – fungicides – unapproved substances according to VO (EG) Nr. 1107/2009 were detected in single cases (e.g. phosalone, chlorfenvinphos,paraoxon-methyl, diphenylamin) Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 11
  • 12. Colony losses during hibernation period 2009/2010 (1) 4 beekeepers supposed losses to be connected with the uptake of potentially contaminated pollen during late summer and autumn of the previous year a) Diagnostic results for pathogens and parasites In beekeeping operations 1 – 3 Varroosis could be identified as the most likely cause for colony losses (Varroa-infestation rate bee samples: 17-39 %; brood samples: 24-56 %) In beekeeping operation 4 severe failures to comply with good apicultural practice were revealed (establishment of very weak mating nucs late in the year, no protective measures against shrews, dysentery and N. ceranae as cofactors), therefore no residue analysis was performed Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 12
  • 13. Colony losses during hibernation period 2009/2010 (2) b) Residue analysis • No residues from insecticidal seed coating materials or other active substances of plant protection products were detected in – bee bread samples (pooled by apiaries) from 3 beekeeping operations – dead bees (pooled sample) from one beekeeping operation • Conclusion: In these 4 cases there are no indications for a contamination of hive products by the investigated analytes Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 13
  • 14. Colony losses during hibernation period 2010/2011 6 beekeepers supposed losses to be connected with the uptake of potentially contaminated pollen during late summer and autumn of the previous year a) Residue analysis of bee bread from dead or very weak colonies • Case 1: Clothianidin < LOQ • Case 2: Imidacloprid < LOQ • Cases 3 - 5: negative • Case 6: no residue analysis was performed (severe case of varroosis: 24 % infestation rate in remaining brood cells) b) Diagnostic results for pathogens and parasites In cases 3 - 6 pests and parasites were most likely the causes for colony winter losses. Conclusions: an exposure to insecticide seed coating material was confirmed for 2 cases of winter losses. Because Imidacloprid is used both as a seed coating and a spray treatment the source for this contaminant remained unclear. Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 14
  • 15. Suspected bee poisoning incidents during bloom of maize or later in the year 2011 Reports from 5 beekeeping operations (5 bee yards) Reasons suspected by beekeepers: • case 1: “spray applikations in seed maize or grape-vine“; residue analysis bees: Imidacloprid 0,0016 mg/kg • case 2: “plant protection activity“: residue analysis bees: Imidacloprid < LOQ (LOQ = 0,001 mg/kg) • cases 3, 4: “contaminated pollen from maize, oilseed-pumpkin, grape-vine“; residue analysis negative • case 5: “unidentified plant protection activity“; residue analysis not possible due to insufficient sample size Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 15
  • 16. Clothianidin – three-years-comparison of residue results for samples of bees and bee bread MELISSA-Vergiftungsverdacht LOD Rückstandsanalysen auf Clothianidin = 0,0002 mg/kg; LOQ = 0,001 mg/kg Matrix: Bienen und Bienenbrot Legend: CLO >= BG CLO < BG CLO n.n. BG = LOQ 100% 0 0 n.n. = not detected 90% 7 15 2 15 80% 2 11 7 9 70% 19 28 60% 9 18 42 29 50% 1 3 1 2 0 1 1 40% 2 30% 24 42 7 3 27 2 20% 2 7 Bees + Bee bread: 10% 0 4 5 9 1 significant decline 2 2 0% 0 0 0 0 2 0 0 0 0 0 0 0 0 in percentage of 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 positive samples B K NOE OOE ST T W V from 2009 - 2011 Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 16
  • 17. Thiamethoxam – three-years-comparison of residue results for samples of bees and bee bread MELISSA-Vergiftungsverdacht LODRückstandsanalysen auf Thiamethoxam = 0,0002 mg/kg; LOQ = 0,001 mg/kg Matrix: Bienen und Bienenbrot Legend: THI >= BG THI < BG THI n.n. BG = LOQ 100% 0 n.n. = not detected 90% 80% 70% 44 60% 16 30 5 25 44 36 50% 1 20 3 1 48 31 4 1 1 40% 30% 12 20% 0 2 2 Bees + Bee bread: 10% 7 2 1 3 0 2 3 1 10 5 significant decline in 0% 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 percentage of positive 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 samples from 2009 - B K NOE OOE ST T W V 2011 Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 17
  • 18. Fipronil + Fipronil-sulfone – three-years- comparison of residue results for samples of bees and bee bread MELISSA-Vergiftungsverdacht MELISSA-Vergiftungsverdacht Fipronil Rückstandsanalysen auf Fipronil Matrix: Bienen und Bienenbrot Rückstandsanalysen auf Fipronil-sulfon Fipronil-sulfone Matrix: Bienen und Bienenbrot FIP >= BG FIP < BG FIP n.n. FIP-S >= BG FIP-S < BG FIP-S n.n. 100% 100% 90% 90% 80% 80% 70% 70% 60% 3 60% 3 28 59 28 62 50 50% 1 20 3 1 6 49 20 38 32 36 50 1 1 50% 1 20 3 1 6 49 20 39 32 37 1 1 40% 40% 30% 30% 0 0 20% 20% 1 1 10% 10% 0 4 0 2 1 1 3 0 2 2 0% 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 1 1 2 0 1 0% 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 B K NOE OOE ST T W V B K NOE OOE ST T W V LOD = 0,0002 mg/kg; LOQ = 0,001 mg/kg Bees: significant decline in percentage of positive samples Legend: BG = LOQ; n.n. = not detected Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 18
  • 19. Imidacloprid – three-years-comparison of residue results for samples of bees and bee bread MELISSA-Vergiftungsverdacht LOD = 0,0002 mg/kg; LOQImidacloprid Rückstandsanalysen auf = 0,001 mg/kg Matrix: Bienen und Bienenbrot IMI >= BG IMI < BG IMI n.n. Legend: 100% 0 BG = LOQ n.n. = not detected 90% 80% 1 70% 14 60% 25 33 46 19 63 48 50% 1 3 1 6 30 40 4 1 1 40% 30% 1 20% 3 3 Bees + Bee bread: 10% 3 4 5 significant increase 2 1 1 1 0% 0 0 0 0 0 0 0 1 0 0 0 2 2 0 0 0 0 0 0 0 0 in percentage of 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 2009 2010 2011 positive samples B K NOE OOE ST T W V from 2009 - 2011 Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 19
  • 20. Exposure of bee yards (%) to insecticidal seed coating materials1 in the years 2009 – 2011 according to results of residue analysis in bees or bee bread Anteil der Bienenstände mit bzw. ohne Rückstandsnachweis für insektizide Saatgutbeizmittel in 1 Clothianidin, Thiamethoxam, Imidacloprid, Fipronil and Bienen oder Bienenbrot Fipronil-sulfone: LOD = 0,0002 untersucht, bzw. 7 Proben nur aufmg/kg * 2011 wurden keine Monitoringstände mg/kg; LOQ = 0,001 "sonstige Pflanzenschutzmittel" untersucht 100% 7 2 90% 5 27 80% 8 70% 66 Bienenstände 13 n.u.** Legend: Bee yards 11 60% neg. BG = LOQ 50% 7 neg = negative < BG 40% 20 28 n.u. = not analysed 30% 55 ≥ BG 2 20% 43 7 *7 samples just analysed for 10% 4 „other active substances of 0% plant protection products; 2009 2010 2011 2009 2010 2011* ** in 2011, no monitoring Suspected poisoning Vergiftungsverdacht Monitoring Monitoring beeyards were investigated; Significant downtrend of exposed bee yards during years of study. Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 20
  • 21. Residue analysis of extracted honey In 2009 9 samples and in 2010 62 samples were analysed for Clothianidin, Thiamethoxam, Imidacloprid: all negative (LOD = 0,0006 mg/kg; LOQ = 0,002 mg/kg) 2011: 13 samples analysed: all were in compliance with the MRL-regulations and fully marketable according to the Austrian legislative framework. Analytes n.d. > LOQ < LOQ Samples (n=) *Clothianidin1 11 0 2 *Thiamethoxam2 12 0 1 *Imidacloprid1 11 1 1 13 *Fipronil2, *Fipronil-sulfone1 13 0 0 Thiacloprid2 5 4 4 Amitraz3 12 1 0 Legend: n.d. = not detectable; LOQ = level of quantification; LOD = level of detection 1 LOQ = 0,0002 mg/kg; LOD = 0,00006 mg/kg 2 LOQ = 0,0005 mg/kg; LOD = 0,00015 mg/kg 3 LOQ = 0,01 mg/kg *for these substances LOD and LOQ, respectively, were lower by a factor of 10 in 2011 compared to the years 2009 and 2010. Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 21
  • 22. Wind conditions at time of maize sowing 2011 „no sowing of insecticidal coated maize and oil pumpkin seed with pneumatic seed drills at a windspeed > 5 m/s (18 km/h)“ Wind-peak values (wsp) 25 20 Windspeed (m/sec) 15 Hours 10 5 Day Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 22
  • 23. Wind conditions in April at time of maize sowing years: 2009, 2010, 2011 „no sowing of insecticidal coated maize and oil pumpkin seed with pneumatic seed drills at a windspeed > 5 m/s (18 km/h)“ Neusiedl/See 2009 - 2011 Location Neusiedl/See Wind-Spitzenwerte 12 Uhr Wind-peak values (wsp) at 12 (a.m.) wsp-12h-2009 wsp-12h-2010 wsp-12h-2011 200 20 Windspeed (m/sec) Windgeschwindigkeit 150 (Zehntelmeter/sec) 15 100 10 50 5 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Tag Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 23
  • 24. Heubach-values of abrasion tests from enforcement (marketing) control samples of insecticidal coated seed batches in 2010 and 2011 0,80 2010 2011 Grenzwert Abrieb 0,75 g / 100.000 permissible abrasion value: 0.75 g/100.000 kernels Maximum Korn 0,70 0,60 Average = 0.35 = 0,35 g / 100.000 Korn Mittelwert g/100.000 kernels Average = 0.17 g/100.000 / 100.000 Korn Mittelwert = 0,17 g kernels 0,50 0,40 0,30 0,20 0,10 0,00 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 24
  • 25. Summary • Results of the MELISSA-project give evidence that in Austria regional clustered bee damages had occurred in the years 2009 – 2011, which were frequently associated with the use of maize and oilseed pumpkin seeds coated with insecticides. • Regulatory measures to prevent honey bee losses due to the exposure of bees to insecticidal seed dressing substances have significantly improved the situation. However, repeatedly observed incidences of honey bee mortality in defined regions suggest their systematic correlation with local factors contributing to increased exposure of bees. • Seed dressing quality and seed drill equipment still need further improvement and sowing of insecticidal dressed seed with pneumatic seed drills has to be avoided under windy conditions • Insecticidal seed dressings should be used solely based on a given risk in plant production with therefore reasoned indication. • An adjustment in the approval procedure is required for risk assessment and approval of insecticidal substances concerning the specific characteristics and possible routes of exposure of bee toxic seed dressing substances. Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 25
  • 26. For further Details, please contact Dr. Rudolf Moosbeckhofer AGES, Institute for Seed and Propagation Material, Phytosanitary Service and Apiculture Department for Apiculture and Bee Protection rudolf.moosbeckofer@ages.at Rudolf Moosbeckhofer, Leopold Girsch - AGES Division Food Security; Presentation: Robert Womastek; SCFCAH - PPP Legislation Meeting, 27-28 Sept 2012 26