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LEADing @ Fraser
The document outlines a school's LEAD program which encourages students to learn with purpose, engage with pride, act with respect, and dare to succeed. It describes the expectations for each value such as being prepared and respecting others. Students who demonstrate these values receive certificates that can be traded for silicone wrist bands representing each value. Earning wrist bands in all four areas results in a LEAD badge to recognize the student's achievement.
8
This study aimed to improve methods for diagnosing yellow leaf syndrome (YLS) in sugarcane, which is caused by two pathogens - a phytoplasma (SCYP) and a luteovirus (SCYLV). For SCYP detection, a new DNA extraction method using FTA cards was found to be more cost-effective and accurate than the previous method. For SCYLV detection, the current tissue-blot immunoassay method was found to be reliable and cheaper than newer PCR-based methods, though PCR allows for pathogen genome sequencing. The improved, lower-cost diagnostic methods are now being used to survey YLS prevalence in South Africa.
12
This study aimed to determine if cofermentation of Lactobacillus buchneri and Lactobacillus diolivorans in sourdough could produce propionic acid and inhibit mold growth on bread for longer shelf life. The researchers found that:
1) Cofermentation of L. buchneri and L. diolivorans in modified MRS broth and sourdough led to production of propionic acid from lactic acid.
2) Up to 48 mM of propionic acid was produced in sourdough with medium ash content flour.
3) Bread made with 20% of this experimental sourdough inhibited the growth of three out of four mold types tested
9
This study isolated and characterized phosphate solubilizing bacteria from the rhizosphere of various crop plants in Korea. Thirteen bacterial isolates were selected that showed phosphate solubilization abilities in vitro. Biochemical and molecular characterization identified the isolates as belonging to the genera Enterobacter, Pantoea, and Klebsiella. Three representative strains had their 16S rDNA sequences deposited in GenBank. The isolates showed the ability to solubilize calcium phosphate and may have potential as inoculants to increase phosphate availability to plants.
5
This document summarizes a study on citrate metabolism by Enterococcus faecium FAIR-E 198 under different pH and substrate conditions. Key findings include:
1) E. faecium FAIR-E 198 was able to metabolize citrate as the sole energy source from pH 5.0 to 7.0, but not at pH 8.0. The main products were acetate, formate, acetoin, and CO2.
2) In the presence of glucose, citrate was cometabolized but did not contribute to growth. More acetate and less acetoin were produced compared to citrate alone.
3) Most citrate was consumed during stationary phase, indicating it was
13
This document summarizes a study that investigated the solubilization of Tunisian rock phosphate by two soil bacteria - Erwinia sp. and Azotobacter sp. The study found that the bacteria were able to solubilize the rock phosphate by producing organic acids. Lower concentrations of rock phosphate and smaller particle sizes led to higher solubilization. Azotobacter sp. solubilized more phosphate than Erwinia sp. The highest solubilization levels were observed between 6-12 days of incubation. Solubilization was correlated with decreased pH and increased acidity over time.
1
The document summarizes a study that analyzed the hydrolytic activity of Penicillium chrysogenum Pg222 on pork myofibrillar proteins. SDS-PAGE analysis revealed that Pg222 extensively hydrolyzed the main myofibrillar proteins except α-actinin. The hydrolysis led to increases in free amino acids, reaching peak values at 84 hours. Pg222 degraded most myofibrillar proteins to undetectable levels by 92 hours, suggesting it could contribute to texture and flavor development in dry-cured meat products through protein breakdown.
Recommended
LEADing @ Fraser
The document outlines a school's LEAD program which encourages students to learn with purpose, engage with pride, act with respect, and dare to succeed. It describes the expectations for each value such as being prepared and respecting others. Students who demonstrate these values receive certificates that can be traded for silicone wrist bands representing each value. Earning wrist bands in all four areas results in a LEAD badge to recognize the student's achievement.
8
This study aimed to improve methods for diagnosing yellow leaf syndrome (YLS) in sugarcane, which is caused by two pathogens - a phytoplasma (SCYP) and a luteovirus (SCYLV). For SCYP detection, a new DNA extraction method using FTA cards was found to be more cost-effective and accurate than the previous method. For SCYLV detection, the current tissue-blot immunoassay method was found to be reliable and cheaper than newer PCR-based methods, though PCR allows for pathogen genome sequencing. The improved, lower-cost diagnostic methods are now being used to survey YLS prevalence in South Africa.
12
This study aimed to determine if cofermentation of Lactobacillus buchneri and Lactobacillus diolivorans in sourdough could produce propionic acid and inhibit mold growth on bread for longer shelf life. The researchers found that:
1) Cofermentation of L. buchneri and L. diolivorans in modified MRS broth and sourdough led to production of propionic acid from lactic acid.
2) Up to 48 mM of propionic acid was produced in sourdough with medium ash content flour.
3) Bread made with 20% of this experimental sourdough inhibited the growth of three out of four mold types tested
9
This study isolated and characterized phosphate solubilizing bacteria from the rhizosphere of various crop plants in Korea. Thirteen bacterial isolates were selected that showed phosphate solubilization abilities in vitro. Biochemical and molecular characterization identified the isolates as belonging to the genera Enterobacter, Pantoea, and Klebsiella. Three representative strains had their 16S rDNA sequences deposited in GenBank. The isolates showed the ability to solubilize calcium phosphate and may have potential as inoculants to increase phosphate availability to plants.
5
This document summarizes a study on citrate metabolism by Enterococcus faecium FAIR-E 198 under different pH and substrate conditions. Key findings include:
1) E. faecium FAIR-E 198 was able to metabolize citrate as the sole energy source from pH 5.0 to 7.0, but not at pH 8.0. The main products were acetate, formate, acetoin, and CO2.
2) In the presence of glucose, citrate was cometabolized but did not contribute to growth. More acetate and less acetoin were produced compared to citrate alone.
3) Most citrate was consumed during stationary phase, indicating it was
13
This document summarizes a study that investigated the solubilization of Tunisian rock phosphate by two soil bacteria - Erwinia sp. and Azotobacter sp. The study found that the bacteria were able to solubilize the rock phosphate by producing organic acids. Lower concentrations of rock phosphate and smaller particle sizes led to higher solubilization. Azotobacter sp. solubilized more phosphate than Erwinia sp. The highest solubilization levels were observed between 6-12 days of incubation. Solubilization was correlated with decreased pH and increased acidity over time.
1
The document summarizes a study that analyzed the hydrolytic activity of Penicillium chrysogenum Pg222 on pork myofibrillar proteins. SDS-PAGE analysis revealed that Pg222 extensively hydrolyzed the main myofibrillar proteins except α-actinin. The hydrolysis led to increases in free amino acids, reaching peak values at 84 hours. Pg222 degraded most myofibrillar proteins to undetectable levels by 92 hours, suggesting it could contribute to texture and flavor development in dry-cured meat products through protein breakdown.
11
Mutations that reduce outer membrane permeability in Escherichia coli lead to increased tolerance of the bacterium to the antimicrobial lactoperoxidase enzyme system. The study identified two E. coli mutants with increased tolerance to lactoperoxidase due to mutations in genes involved in lipopolysaccharide synthesis. These mutants had reduced amounts of porins in their outer membrane, indicating decreased outer membrane permeability. Knockout mutants of specific porin genes also showed increased tolerance, suggesting the antimicrobial activity of lactoperoxidase relies on uptake of its oxidized substrates through porins.
10
This document is the journal of the American Society of Sugar Cane Technologists from June 2003. It includes summaries of various research papers on sugarcane agriculture and manufacturing. The journal provides information on the executive committee members of the society and lists honorary members. It also recognizes the winners of the 2002 Denver T. Loupe Best Presentation Awards. Tables of contents and lists of agricultural and manufacturing research abstracts are included at the end.
7
The document surveys the chromium tolerance and bioaccumulation abilities of 51 yeast strains from various genera. The strains were grown in media supplemented with either chromium(III) or chromium(VI) at inhibitory concentrations. Cellular chromium content was then measured. Chromium(III) tolerance ranged from 0.25-5 mM among strains, while chromium(VI) tolerance ranged from 0.1-0.5 mM, with strains generally more sensitive to chromium(VI). Cellular chromium levels varied significantly between strains but followed a general trend of mid-range accumulation of chromium(III) and low-level accumulation of chromium(VI). Electron microscopy showed chromium accumulated in the cytoplasm and organelles of cells.
6
This study examined the growth kinetics of Bifidobacterium animalis DN-173 010 on different carbohydrates as energy sources, including inulin-type fructans. Small- and large-scale fermentations showed that B. animalis grew poorly on monosaccharides but preferentially metabolized shorter oligofructose chains, depleting them before metabolizing longer chains. Acetic acid was the major metabolite produced, along with initial high levels of lactic acid and later formic acid production, suggesting a shift in sugar metabolism for growth on oligofructose.
2
This document summarizes a study on the applicability of using Enterococcus faecium strains that produce bacteriocins as co-cultures in Cheddar cheese manufacture. Specifically, it investigates the growth and bacteriocin production of E. faecium RZS C5 during fermentation in complex media and milk under various conditions relevant to cheesemaking. It also tests the application of E. faecium RZS C5 and E. faecium DPC 1146 as co-cultures during pilot-scale Cheddar cheese production. The results show that bacteriocin production occurs throughout growth and is stable during cheese ripening, indicating these strains could combat contamination during cheesemaking.
Clase lc.comunicacion cientifica
El documento presenta un taller sobre la metodología de la investigación científica. Explica que la estructura de un trabajo científico experimental incluye el título, autores, introducción, antecedentes, materiales y métodos, resultados, discusión, conclusiones y bibliografía. Además, detalla los pasos para la comunicación del trabajo científico, incluyendo la estructura, contenido y estilo de los resúmenes y manuscritos, así como las normas para presentaciones en congresos y publicaciones.
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11
Mutations that reduce outer membrane permeability in Escherichia coli lead to increased tolerance of the bacterium to the antimicrobial lactoperoxidase enzyme system. The study identified two E. coli mutants with increased tolerance to lactoperoxidase due to mutations in genes involved in lipopolysaccharide synthesis. These mutants had reduced amounts of porins in their outer membrane, indicating decreased outer membrane permeability. Knockout mutants of specific porin genes also showed increased tolerance, suggesting the antimicrobial activity of lactoperoxidase relies on uptake of its oxidized substrates through porins.
10
This document is the journal of the American Society of Sugar Cane Technologists from June 2003. It includes summaries of various research papers on sugarcane agriculture and manufacturing. The journal provides information on the executive committee members of the society and lists honorary members. It also recognizes the winners of the 2002 Denver T. Loupe Best Presentation Awards. Tables of contents and lists of agricultural and manufacturing research abstracts are included at the end.
7
The document surveys the chromium tolerance and bioaccumulation abilities of 51 yeast strains from various genera. The strains were grown in media supplemented with either chromium(III) or chromium(VI) at inhibitory concentrations. Cellular chromium content was then measured. Chromium(III) tolerance ranged from 0.25-5 mM among strains, while chromium(VI) tolerance ranged from 0.1-0.5 mM, with strains generally more sensitive to chromium(VI). Cellular chromium levels varied significantly between strains but followed a general trend of mid-range accumulation of chromium(III) and low-level accumulation of chromium(VI). Electron microscopy showed chromium accumulated in the cytoplasm and organelles of cells.
6
This study examined the growth kinetics of Bifidobacterium animalis DN-173 010 on different carbohydrates as energy sources, including inulin-type fructans. Small- and large-scale fermentations showed that B. animalis grew poorly on monosaccharides but preferentially metabolized shorter oligofructose chains, depleting them before metabolizing longer chains. Acetic acid was the major metabolite produced, along with initial high levels of lactic acid and later formic acid production, suggesting a shift in sugar metabolism for growth on oligofructose.
2
This document summarizes a study on the applicability of using Enterococcus faecium strains that produce bacteriocins as co-cultures in Cheddar cheese manufacture. Specifically, it investigates the growth and bacteriocin production of E. faecium RZS C5 during fermentation in complex media and milk under various conditions relevant to cheesemaking. It also tests the application of E. faecium RZS C5 and E. faecium DPC 1146 as co-cultures during pilot-scale Cheddar cheese production. The results show that bacteriocin production occurs throughout growth and is stable during cheese ripening, indicating these strains could combat contamination during cheesemaking.
Clase lc.comunicacion cientifica
El documento presenta un taller sobre la metodología de la investigación científica. Explica que la estructura de un trabajo científico experimental incluye el título, autores, introducción, antecedentes, materiales y métodos, resultados, discusión, conclusiones y bibliografía. Además, detalla los pasos para la comunicación del trabajo científico, incluyendo la estructura, contenido y estilo de los resúmenes y manuscritos, así como las normas para presentaciones en congresos y publicaciones.