1.plant tissue culture history

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1.plant tissue culture history

  1. 1. Cell and TissueCulture
  2. 2. T.C.Refers to technique ofgrowing plantcells, tissues, organs, seedsor other plant parts in asterile environment on anutrient medium In vitro
  3. 3. History Haberlandt Realized that asepsis was necessary when culturemedia enriched with organic substances metabolized bymicroorganisms In 1898 he cultured single cells on Knop’s salt solution In his cultures the cell were able to synthesize starch as wellas increase in size He failed in his goal to induce cell division
  4. 4. HistoryDespite of failures Haberlandt proposed that plant cellsare totipotentTotipotency is ability of individual cells to express thephenotype of whole plant from which it is derivedHaberlandt is regarded as father of plant tissue culture
  5. 5. HistoryHanning(1904) Embryo culture of selectedcrucifersRobbins (1922), Kotte In Vitro culture of root tipsLaibach (1925) Use of embryo culture techniquein inter-specific crosses of Linum
  6. 6. HistoryWhite (1934-37) Successful culture of tomato rootsimportance of vitamins in growth mediaSnow, Gautheret (1934-1939) Importance of auxinas growth regulatorGautheret, Nobecourt, White (1939) Successfulestablishment of continuously growing cultures.
  7. 7. HistoryVan Overbeek (1941) Use of coconut milkcontaining a cell division factor first time in DaturaSkoog and Tsui (1951), Miller et al (1955), In vitrocell differentiation, discovery of KinetinMorel and Martin (1952) Application of micrografting to obtain virus free plants
  8. 8. HistoryMuir (1953) Isolation and culture of single cellsfrom plantsSkoog and Miller (1957) Discovery of principle ofhormonal control of the organ formation in tissueculture
  9. 9. HistoryReinert and Steward (1958-59) First report onsomatic embryogenesisJones et al (1960) Hanging drop method of cellcultureBergmann (1960) Bergmann cell culturetechnique
  10. 10. HistoryE C Cocking (1960) Isolation of protoplasts byenzymatic degradation methodMurashige and Skoog (1962) Development ofMS mediaGuha and Maheshwawari (1964) Production ofFirst haploid plant by anther culture
  11. 11. HistoryPower et al (1970) Protoplast fusionTakabe et al (1971)Rgeneration of first plantfrom protoplastCarlson et al (1972) First report on inter-specifichybridization through protoplast fusion

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