2. METHODS
Gas chromatography of ethane, ethene, and
pentane in expired breath
Analysis of age-related
pigments(e.g., lipofuscin, ceroid) based on
characteristic fluorescence and emission spectra
Spectrophotometry of conjugated dienes at 233 nm
Colorimetry and fluorimetry of MDA and other
chromogens based on the thiobarbituric acid (TBA)
reaction
The measurement of various products by high
performance liquid chromatography (HPLC)
3. The ThioBarbituric Acid (TBA) reaction, quantified by
colorimetry or fluorimetry, is the method most widely
used for studying lipid peroxidation in both laboratory
animals and in humans with disorders
The reaction products were also analyzed for MDA by
high-performance liquid chromatography (HPLC)
4. MDA + 2 TBA Stable Pink to Red
colour that
absorbs maximally at
532 nm
5.
6. Sensitivity:
Fourfold greater than that of Yagi’s
Specificity:
Same specificity problems as Yagi’s
Practicability:
More practicable and better adapted
to measurements of large series of samples
Faster than the HPLC technique and less
expensive.