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191 spio and t2 effect

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SHAPE Society

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191 spio and t2 effect

  1. 1. SPIO and T2 Effect In vitro study to show the effect of macrophage SPIO uptake on their T2 relaxation time
  2. 2. Protocol:  We used 8 flasks of CBM macrophages.  After preparing the cells, Feridex was added with the proper concentration to each labeled tube.  Incubation was done at 37 C.  For each time, pellet the tubes at 1000 rpm’s for 5 min.  Washed with 1X PBS for 5 min 3 times.  Resuspended in 2% paraformaldehyde, to fix the cells.
  3. 3. time concentration 20 Min 1 Hour 6 Hours 24 Hours 50µl 100µl 250µl 500µl control control Expected T2 Reduction Effect
  4. 4. Macrophage Uptake of Feridex After 20 Min Shown by T2 Reduction 0 10 20 30 40 50 60 70 80 90 50 100 250 500 control control 20 min Concentration µl
  5. 5. 0 10 20 30 40 50 60 70 80 90 50 100 250 500 control control 60 min Macrophage Uptake of Feridex After 60 Min Shown by T2 Reduction Concentration µl
  6. 6. 0 10 20 30 40 50 60 70 80 90 50 100 250 500 control control 6 Hours Macrophage Uptake of Feridex After 6hr Shown by T2 Reduction Concentration µl
  7. 7. 0 10 20 30 40 50 60 70 80 90 50 100 250 500 control control 24 Hours Macrophage Uptake of Feridex After 24hr Shown by T2 Reduction Concentration µl
  8. 8. 0 10 20 30 40 50 60 70 80 90 50 100 250 500 control control 20 min 60 min 6 hours 24 hours Macrophage Uptake of Feridex with Time and Concentration Shown by T2 Reduction Concentration µl
  9. 9. 0 10 20 30 40 50 60 70 80 90 20 Min 60 Min 6 Hours 24 Hours 50 100 250 500 control control Macrophage Uptake of Feridex with Concentration and Time Shown by T2 Reduction µl
  10. 10. •SQUIDS are extremely sensitive detectors for magnetic fields Summary: •They utilize 3 quantum mechanical effects: Zero resistance, Flux quantization, and Josephson effect. What can we do with it? SQUIDs can be used to as a noninvasive screening test for detecting super paramagnetic or paramagnetic particles in site of inflammation, infection or injury in body Examples: Myocardial infarction atherosclerotic plaque (vulnerable plaque)
  11. 11. Myocardial infarction Acute MI Q-wave Non-Q wave Short axis cine Short-axis contrast 50 y/o male admitted with chest pain and precordial ST segment elevation. Patient was treated with primary angioplasty but Q-waves eventually developed in leads 1,L, avL. • Peak CK: 5912; MB: 792 • Cath Report: 100% occluded left circumflex coronary artery which was opened with PTCA and stented. • MRI: performed 2 weeks after acute MI. The lateral wall is akinetic (cine MRI) and has transmural hyperenhancement (ceMRI).
  12. 12. Myocardial infarction Acute MI Q-wave Non-Q wave Case history: • 52 y/o male admitted with minor chest pain and nonspecific ECG findings. Echocardiogram was normal but the patient eventually ruled in for a non Q-wave MI. • Peak CK: 560; MB: 62 • Cath Report: 100% occluded 1st Diagonal off the LAD. No other significant CAD was found. • MRI: performed 6 days after acute MI. Wall motion is normal (cine MRI) but a small non-transmural region of hyperenhancement is seen in the anterolateral wall (ceMRI). short-axis cine short-axis contrast
  13. 13. Insert Gd vs SPIO charts here

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