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Soil bacterial community structure remains stable with
rising temperature in Hawaiian montane wet forests
Paul C. Selmants...
Bacteria are the most abundant
and diverse organisms on Earth
How will soil bacteria respond
to climate warming?
How will soil bacteria respond
to climate warming?
Climatic controls on bacterial
distribution poorly resolved
How will soil bacteria respond
to climate warming?
Climatic controls on bacterial
distribution poorly resolved
Shift in di...
Hawaii Mean Annual Temperature Gradient
800-1600 m (13-18.2 °C)
A model study system:
- Constant plant spp. composition
- ...
Hawaii Mean Annual Temperature Gradient
800-1600 m (13-18.2 °C)
A model study system:
- Constant plant spp. composition
- ...
Workflow
DNA extraction
(8 samples/MAT plot)
16S rDNA
amplification
454
Pyrosequencing
Workflow
DNA extraction
(8 samples/MAT plot)
16S rDNA
amplification
454
Pyrosequencing
Sequence
processing
(QIIME pipeline)
...
●
●
●●
●●●●●
0
500
1000
1500
2000
13 14 15 16 17 18
Mean annual temperature (°C)
EstimatedOTUrichness
No effect of rising ...
No effect of rising MAT
on phylogenetic diversity
●
●
●●●
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●●●
0
20
40
60
80
13 14 15 16 17 18
Mean annual temperature (°...
No effect of rising MAT on
composition at phylum level
0
25
50
75
100
13 13.8 15.1 15.5 16.1a 16.1b 16.7 17.3 18.2
Mean an...
Weak effect of rising MAT
on OTU composition
R2 = 0.03, p = 0.07 (PERMANOVA)
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Why do soil bacterial communities
remain stable with rising MAT?
Increase in abundance
Increase in activity
Decrease in do...
Conclusions
“Rising tide lifts all boats”
Warming alone may be
insufficient to alter
community structure
!(
!(
!(
!(
!(
!(
...
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Climate warming and soil bacterial diversity in tropical montane wet forests

Talk presented at Ecological Society of America meeting in Sacramento, CA - August 2014.

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Climate warming and soil bacterial diversity in tropical montane wet forests

  1. 1. Soil bacterial community structure remains stable with rising temperature in Hawaiian montane wet forests Paul C. Selmants, Karen L. Adair, Creighton M. Litton, Christian P. Giardina & Egbert Schwartz
  2. 2. Bacteria are the most abundant and diverse organisms on Earth
  3. 3. How will soil bacteria respond to climate warming?
  4. 4. How will soil bacteria respond to climate warming? Climatic controls on bacterial distribution poorly resolved
  5. 5. How will soil bacteria respond to climate warming? Climatic controls on bacterial distribution poorly resolved Shift in diversity could alter biogeochemical cycling
  6. 6. Hawaii Mean Annual Temperature Gradient 800-1600 m (13-18.2 °C) A model study system: - Constant plant spp. composition - Constant disturbance histories - Constant substrate age & type - Constant soil water balance !( !( !( !( !( !( !( !( !( 18.2 °C 17.3 °C 16.7 °C 16.1 °C 16.1 °C 15.5 °C 15.1 °C 13.8 °C 13.0 °C ¯0 2 41 Kilometers Forest Reserve Natural Area Reserve National Wildlife Refuge Island of Hawaii
  7. 7. Hawaii Mean Annual Temperature Gradient 800-1600 m (13-18.2 °C) A model study system: - Constant plant spp. composition - Constant disturbance histories - Constant substrate age & type - Constant soil water balance !( !( !( !( !( !( !( !( !( 18.2 °C 17.3 °C 16.7 °C 16.1 °C 16.1 °C 15.5 °C 15.1 °C 13.8 °C 13.0 °C ¯0 2 41 Kilometers Forest Reserve Natural Area Reserve National Wildlife Refuge Island of Hawaii Long-term, whole-ecosystem response to rising MAT
  8. 8. Workflow DNA extraction (8 samples/MAT plot) 16S rDNA amplification 454 Pyrosequencing
  9. 9. Workflow DNA extraction (8 samples/MAT plot) 16S rDNA amplification 454 Pyrosequencing Sequence processing (QIIME pipeline) Rarefaction (2200 seq./sample) Data analysis (Diversity, composition)
  10. 10. ● ● ●● ●●●●● 0 500 1000 1500 2000 13 14 15 16 17 18 Mean annual temperature (°C) EstimatedOTUrichness No effect of rising MAT on OTU richness
  11. 11. No effect of rising MAT on phylogenetic diversity ● ● ●●● ● ●●● 0 20 40 60 80 13 14 15 16 17 18 Mean annual temperature (°C) Phylogeneticdiversity
  12. 12. No effect of rising MAT on composition at phylum level 0 25 50 75 100 13 13.8 15.1 15.5 16.1a 16.1b 16.7 17.3 18.2 Mean annual temperature (°C) Relativeabundance(%) Phylum Proteobacteria Acidobacteria Actinobacteria Chloroflexi Candidate WPS−2 Planctomycetes Other
  13. 13. Weak effect of rising MAT on OTU composition R2 = 0.03, p = 0.07 (PERMANOVA) ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ●● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● NMDS Axis 1 NMDSAxis2 13 14 15 16 17 18 MAT (°C)
  14. 14. Why do soil bacterial communities remain stable with rising MAT? Increase in abundance Increase in activity Decrease in dormancy !( !( !( !( !( !( !( !( !( 18.2 °C 17.3 °C 16.7 °C 16.1 °C 16.1 °C 15.5 °C 15.1 °C 13.8 °C 13.0 °C ¯0 2 41 Kilometers Forest Reserve Natural Area Reserve National Wildlife Refuge Island of Hawaii
  15. 15. Conclusions “Rising tide lifts all boats” Warming alone may be insufficient to alter community structure !( !( !( !( !( !( !( !( !( 18.2 °C 17.3 °C 16.7 °C 16.1 °C 16.1 °C 15.5 °C 15.1 °C 13.8 °C 13.0 °C ¯0 2 41 Kilometers Forest Reserve Natural Area Reserve National Wildlife Refuge Island of Hawaii @biogeocycle

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