Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

Bioserve Space Technologies

46 views

Published on

Webinar in Support of NSF Solicitation NSF 19-509

Published in: Science
  • Be the first to comment

  • Be the first to like this

Bioserve Space Technologies

  1. 1. Speaker: Stefanie Countryman Associate Director countrym@colorado.edu 303-735-5308 NSF/CASIS Webinar 11/05/2018 NSF Tissue Engineering and Mechanobiology Research Onboard the International Space Station
  2. 2. Our Mission Since 1987: Collaborate with industry, academia and government to conduct high impact, high quality space life sciences research • Center within the University of Colorado Boulder • Established 1987 • First flight STS-37 1991 • Over 60 space flight missions • Extensive space life sciences research and space life sciences flight hardware design expertise • Small Organization - ~15 FTEs • Research faculty, staff engineers, administration personnel and students • Focus on quality science www.colorado.edu/engineering/BioServe/ Facebook Twitter (@BioServeST) Who we are
  3. 3. What we do n Science support, technical guidance n Feasibility analysis, experiment design n Hardware design, build n Extensive suite space flight hardware n Hardware selection and training n Payload manifest n Payload mission management n Mission integration n All NASA Documentation Requirements n Ground test facilities/support n Support extensive ground based testing n Software/firmware development n Crew procedure writing and crew training n On-orbit experiment operations planning n Real time payload/experiment operations n Dedicated remote payload operations control center n Launch and landing site support
  4. 4. Our history • BioServe supported life science experiments on over 60 space flight missions • Russian Mir Space Station • 39 space shuttle missions • Soyuz/Progress • Japan’s HTV • SpaceX Dragon • NG Cygnus • International Space Station Since 2001 • Hundreds of individual science investigations • Muscle atrophy and bone loss • Mammalian cell and tissue culture • Microbial studies– virulence and antibiotic effectiveness, secondary compound production • Small organisms • Model organisms – bacteria, yeast, fruit flies, nematodes • Rodent Research – animal disease models • Supported most areas of life science research
  5. 5. Recent science supported • Mammalian Cell Culture/Tissue Engineering • Osteomics - SpaceX-9 • Gravitational Regulation of Osteoblast Genomics and Metabolism • Heart Cells - SpaceX-9 • Cardiomyocytes - cardiac function, structure and gene expression in space • MESC - Spacex-10 • Expansion of human MSCs and iPSCs • CSC - SpaceX-11 • Cardiovascular progenitor cells (neonatal and adult) expression of microRNAs and specific genes • Lung Tissue - SpaceX-12 • hAEC, epithelial cells and macrophages grown on a scaffold of human acellularized lung tissue • Synthetic Bone - SpaceX-13 • Examined Tetranite™ (TN), a bone adhesive developed by LaunchPad Medical (LPM). • M3D - OA-7 • Magnetic system for 3d cell culturing in space • ADC - OA7 • 3D Spheroids for chemotherapeutic screening • Metabolic Tracking - SpaceX-14 • Autobioluminescent human tissue culture as a tool for streamlining microgravity-based drug discovery assays. (SpaceX-14) • Angiex - SpaceX-15 • Cultured endothelial cells in microgravity as a model system for evaluation of cancer therapy toxicity • Sampling of Upcoming Mammalian Cell Culture Experiments • NIH NCATS – Kidney Cells (Nortis Chip – UW) – February 2019 • NASA – Micro-15 – UMINN - Embryoid Bodies – May 2019 • CASIS – Sanofi – Immune cells – October 2019 • CASIS/Mass Challenge – Cellino – iPSCs – October 2019 • NIH NCATS – Engineered Heart Tissue (UW) – Early 2020 • NIH NCATS – Engineered Heart Tissue (Stanford) – Early 2020
  6. 6. Results oriented • Over 375 refereed journal articles • High quality science is our philosophy • Conduct experiment in space as close as possible to Earth based lab • Support both flight and ground controls • Microgravity simulations (clinostat) when beneficial and possible • Data result of microgravity exposure rather than a result of hardware limitations or other challenges associated with conducting science in space.
  7. 7. Unique hardware facilities on ISS • SABL – On orbit incubator • 37°C, 5% CO2 (+/- 0.1C) • Temperature range -5°C to 43°C • Atmosphere composition control ambient to 10%CO2 • Two USB ports to support powered USB devices • Two Ethernet LAN connections available • Switchable 28vdc and 5vdc power • Three units operating on ISS now • CGBA – On orbit incubator • On board ISS since 2001 • Temperature range of -16°C to 37°C • Supports powered/unpowered experiments • Transportation to/from ISS for temperature control • Nikon Eclipse TS100 Microscope – On orbit • Standard, phase contrast, inverted microscope • 40X, 20X, 10X, 4X, 2X objectives • GigE camera system for still and video imagery
  8. 8. Cell and Tissue Culture Hardware • BioCells • Supports suspension or adherent cultures • Single 6, or 12 well options • Passive gas exchange through membranes • Inlet, outlet and sampling ports for fluid exchanges • Launch active or frozen cultures • Experience thawing cultures on orbit • Experience designing automated systems • Continuous perfusion • Automated sampling • Inoculation • Other options available for suspended cell and tissue culture
  9. 9. Other relevant expertise •Translating ground based experiments to space flight •Microfluidics •Sterile technique – lab and loading hardware •Biocompatibility •Fluid exchanges including media, preservation, fixation •Access to cold stowage assets (-80°C to +4°C) during ascent, descent and on orbit for maintenance of samples until return to space
  10. 10. BioServe • Highly experienced team • Large inventory of available flight hardware • Can modify or design new hardware, as needed • Full knowledge of all details required to successfully fly life science in space • Allow PI team to focus on science, not on flight process requirements • BioServe team is dedicated to success in space!
  11. 11. Thank you!

×