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Bioserve Space Technologies

Webinar in Support of NSF Solicitation NSF 19-509

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Bioserve Space Technologies

  1. 1. Speaker: Stefanie Countryman Associate Director 303-735-5308 NSF/CASIS Webinar 11/05/2018 NSF Tissue Engineering and Mechanobiology Research Onboard the International Space Station
  2. 2. Our Mission Since 1987: Collaborate with industry, academia and government to conduct high impact, high quality space life sciences research • Center within the University of Colorado Boulder • Established 1987 • First flight STS-37 1991 • Over 60 space flight missions • Extensive space life sciences research and space life sciences flight hardware design expertise • Small Organization - ~15 FTEs • Research faculty, staff engineers, administration personnel and students • Focus on quality science Facebook Twitter (@BioServeST) Who we are
  3. 3. What we do n Science support, technical guidance n Feasibility analysis, experiment design n Hardware design, build n Extensive suite space flight hardware n Hardware selection and training n Payload manifest n Payload mission management n Mission integration n All NASA Documentation Requirements n Ground test facilities/support n Support extensive ground based testing n Software/firmware development n Crew procedure writing and crew training n On-orbit experiment operations planning n Real time payload/experiment operations n Dedicated remote payload operations control center n Launch and landing site support
  4. 4. Our history • BioServe supported life science experiments on over 60 space flight missions • Russian Mir Space Station • 39 space shuttle missions • Soyuz/Progress • Japan’s HTV • SpaceX Dragon • NG Cygnus • International Space Station Since 2001 • Hundreds of individual science investigations • Muscle atrophy and bone loss • Mammalian cell and tissue culture • Microbial studies– virulence and antibiotic effectiveness, secondary compound production • Small organisms • Model organisms – bacteria, yeast, fruit flies, nematodes • Rodent Research – animal disease models • Supported most areas of life science research
  5. 5. Recent science supported • Mammalian Cell Culture/Tissue Engineering • Osteomics - SpaceX-9 • Gravitational Regulation of Osteoblast Genomics and Metabolism • Heart Cells - SpaceX-9 • Cardiomyocytes - cardiac function, structure and gene expression in space • MESC - Spacex-10 • Expansion of human MSCs and iPSCs • CSC - SpaceX-11 • Cardiovascular progenitor cells (neonatal and adult) expression of microRNAs and specific genes • Lung Tissue - SpaceX-12 • hAEC, epithelial cells and macrophages grown on a scaffold of human acellularized lung tissue • Synthetic Bone - SpaceX-13 • Examined Tetranite™ (TN), a bone adhesive developed by LaunchPad Medical (LPM). • M3D - OA-7 • Magnetic system for 3d cell culturing in space • ADC - OA7 • 3D Spheroids for chemotherapeutic screening • Metabolic Tracking - SpaceX-14 • Autobioluminescent human tissue culture as a tool for streamlining microgravity-based drug discovery assays. (SpaceX-14) • Angiex - SpaceX-15 • Cultured endothelial cells in microgravity as a model system for evaluation of cancer therapy toxicity • Sampling of Upcoming Mammalian Cell Culture Experiments • NIH NCATS – Kidney Cells (Nortis Chip – UW) – February 2019 • NASA – Micro-15 – UMINN - Embryoid Bodies – May 2019 • CASIS – Sanofi – Immune cells – October 2019 • CASIS/Mass Challenge – Cellino – iPSCs – October 2019 • NIH NCATS – Engineered Heart Tissue (UW) – Early 2020 • NIH NCATS – Engineered Heart Tissue (Stanford) – Early 2020
  6. 6. Results oriented • Over 375 refereed journal articles • High quality science is our philosophy • Conduct experiment in space as close as possible to Earth based lab • Support both flight and ground controls • Microgravity simulations (clinostat) when beneficial and possible • Data result of microgravity exposure rather than a result of hardware limitations or other challenges associated with conducting science in space.
  7. 7. Unique hardware facilities on ISS • SABL – On orbit incubator • 37°C, 5% CO2 (+/- 0.1C) • Temperature range -5°C to 43°C • Atmosphere composition control ambient to 10%CO2 • Two USB ports to support powered USB devices • Two Ethernet LAN connections available • Switchable 28vdc and 5vdc power • Three units operating on ISS now • CGBA – On orbit incubator • On board ISS since 2001 • Temperature range of -16°C to 37°C • Supports powered/unpowered experiments • Transportation to/from ISS for temperature control • Nikon Eclipse TS100 Microscope – On orbit • Standard, phase contrast, inverted microscope • 40X, 20X, 10X, 4X, 2X objectives • GigE camera system for still and video imagery
  8. 8. Cell and Tissue Culture Hardware • BioCells • Supports suspension or adherent cultures • Single 6, or 12 well options • Passive gas exchange through membranes • Inlet, outlet and sampling ports for fluid exchanges • Launch active or frozen cultures • Experience thawing cultures on orbit • Experience designing automated systems • Continuous perfusion • Automated sampling • Inoculation • Other options available for suspended cell and tissue culture
  9. 9. Other relevant expertise •Translating ground based experiments to space flight •Microfluidics •Sterile technique – lab and loading hardware •Biocompatibility •Fluid exchanges including media, preservation, fixation •Access to cold stowage assets (-80°C to +4°C) during ascent, descent and on orbit for maintenance of samples until return to space
  10. 10. BioServe • Highly experienced team • Large inventory of available flight hardware • Can modify or design new hardware, as needed • Full knowledge of all details required to successfully fly life science in space • Allow PI team to focus on science, not on flight process requirements • BioServe team is dedicated to success in space!
  11. 11. Thank you!