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B Natural Bbook

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Presenting B Natural and its exclusive way to produce high quality propolis extracts.
B Natural extracts are obtained through patented M.E.D.® technology, that allows to retain all the complexity of propolis raw material, while submitting it to a strict scientific control. Thanks to this exclusive technology, B Natural extracts offer a complete, identified and characterized polyphenolic complex.

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B Natural Bbook

  1. 1. 1Bbook PROPOLIS AND BEEKEEPING DERIVATIVES PROPOLIS AND BEEKEEPING DERIVATIVES M.E.D.® PROPOLIS The natural evolution of Propolis extract Characterized Polyphenols Complex
  2. 2. BBOOK Propolis Man and propolis B Natural From Propolis... ... to M.E.D.® Propolis The Patented Method Different extracts, same active complex Upper respiratory tract Intimate care Oral cavity Gastrointestinal tract Skin care Product list Bibliography 5 7 9 11 13 14 15 17 21 25 29 33 35 41
  3. 3. Propolis is a unique product in nature: it is the result of a cooperation between flora and fauna. It is produced by bees, who collect resins secreted by plants and work them out with their own salivary enzymes to obtain Propolis. Bees are extraordinary animals, essential to human life wich perfectly know the numerous biological activities of resins produced by plants. They are able to transform a huge number of simple raw materials like for instance pollen and nectar into highly complex substances like royal jelly and honey. Plants have developed defense systems in order to protect themselves from challenges of the outside world (atmospheric agents and microorganisms). Resins have a crucial role in preserving the health and life of the plants. They represent in fact the immune defenses of plants: if a tree is cut or damaged, it starts secreting resins to repair the damage. Man has been studying plants over the years and he has learnt to make the most out of the antibacterical properties of resins. Why do bees produce Propolis? To give an answer to this question, we have to introduce you to the world of bees: the beehive. The beehive is actually a town with approximately 50.000 mem- bers, a humidity of 90% and an internal temperature of about 37°C. These are usually the optimal conditions for the development of bacteria and the spread of diseases. Despite these extremely adverse conditions, the beehive is one of the most aseptic environments in nature due to the presence of Propolis which has antimicrobial, antioxidant and anti-inflammatory properties. Propolis is used to cover the cells to protect the brood and the young larvae, as a sealing material to close any possible openings of the beehive to the external environment or, in addition, to cover the remains of predators, like lizards or small mice, killed after entering the beehive to steal honey, but too heavy to be brought outside once dead. The conditions in the beehive could reflect the ones in human body: Propolis is the immune system of bees. PROPOLIS
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  6. 6. An ancient story Since dawn of time man has been using the products offered by nature to heal and survive. Bee products, especially honey and Propolis, have found for centuries a widespread use in traditional medicine. Egyptians used Propolis to stop the decomposition of bodies during the mummification process. Greeks were the first to transform beekeeping into farming. Propolis and bees were studied by Aristotele and Hippocrates used to prescribe Propolis to heal ulcers and various kind of disorders both inter- nal and external. Romans were also fasci- nated by the world of bees. It was in fact Plinio in his Natural Hystory to coin the name Propolis: PRO=before + POLIS = town. The amazing properties of bee products were highly recognized for centuries in Arab cultures and they are even mentio- ned in several verses of the Koran. With the advent of modern medicine and the synthesis of antibiotics drugs, the use of Propolis has been reduced in occidental countries. Due to the improper use of antibiotics and the consequential phenomenon of antibiotic resistance as global problem, the interest on Propolis comes back. Bacteria are smarter than man. Bacteria are constantly evolving to ensure their survival. They adapt to the environment, the weather and also the tools put in place by man to eradicate them. Nature that fights nature. Propolis contains more than 300 active molecules that carry out a synergistic action in the inhibition of bacterial growth of many pathogenic strains. The high number of molecules, working in synergy, prevent the development of bacterial resistance. MAN AND PROPOLIS
  7. 7. B NATURAL Inspired by the outstanding work of bees, B Natural has the aim to create unique products. Thanks to 30 years’ experience in extraction and transforma- tion of natural products the B Natural mission is the evolution of bee products, especially Propolis, from traditional remedies to defined and active nutra- ceutical ingredients. Since the composition of Propolis, and then its yeld and its active compounds, varies according to climatic condition, geographical and botanical origin the first challenge of B Natural was the improvement of extraction method to standardize the final extracts. We have therefore developed and patent the Dynamic Multi Extraction method (M.E.D.®) able to purify and extract the actives of Propolis thus creating exclusive and characterized products. M.E.D.® Propolis extracts are bioavailable and hydro-dispersible. Thanks to the characterization and stan- dardization of all Polyphenols identified in HPLC-ESI-MS, we can reproduce the same quality in all our extracts. The expe- rience gathered over the years has deve- loped a specific and scientific approach which enables us to make available and prove the activity of our characterized polyphenols complex of M.E.D.® Propolis.
  8. 8. QUALITY PRODUCTION B Natural is specialized in the production of Propolis extracts obtained with Dyna- mic Multi Extraction method. We have a line of extraction of Propolis through a system of reactors, concentrators, cooled decanters and automated filtering systems able to produce more than 300 tons of Propolis extracts. There are also three productive lines for the production of our main extracts: The unique co-operation between Flora and Fauna has also a critical aspect as Propolis can be subject to contamina- tions from both the plant world (pestici- des, fertilizers, etc.) and the animal world (drugs, antibiotics, etc.). B Natural has been able to turn this critical aspect into a strength! Thanks to a strict internal procedure of controls, we can ensure to our customers the quality and safety of our products. Amongst our analysis, we pay special attention to: o Pesticides o Antibiotics o Aflatoxins o Heavy Metals o PAH In order to develop new production processes satisfying and anticipating customers’ needs, B Natural always looks for new technologies and raw materials able to increase its products and services much more efficient. For these reasons, B Natural invests an important part of its turnover in R&D activities collaborating with University around the world. B Natural is focused on continuous indu- strial innovations, in order to deliver products with an ever-growing perfor- mance. 10 Raw material suppliers from various countries are carefully selected and validated. Duplicate analyses are performed on each batch by certified international laboratories. a line with a spray drying equipment for the production of hydro-dispersible Propolis powder extracts ESIT®, Honey Powder, Propolsave®, Flavoxale®, Propol-fenol® and Honeyna-C. a line for the production of non-alcoho- lic extracts such as the hydro-glyceric extracts GREIT®, oily extracts POLE®, glycolic extracts and phyto-prepara- tions in bulk ready to pack based on our liquid extracts a line for the production of granulated powder extracts. B Natural industrial plants work in accor dance with HACCP procedures. B Natural is ISO 9001-2015, ORGANIC certified and GMP compliant.
  9. 9. FROM PROPOLIS... Thanks to its patented Dynamic Multi Extraction process (M.E.D.®), B Natural has won the challenge of reconciling nature and science, producing extracts of Propolis able to retain all its natural power. THE HIVE IMMUNE SYSTEM RICHNESS AND VARIABILITY Bees use Propolis as a sanitizing sealant and waterproofing agent. It is the perfect material to repair combs and make the entrance of the hive weathertight and easier to defend. Bees keep their hive from being infected by coating the inside of the hive with Propolis, thus preventing the spread of bacteria and fungi that would be detrimental to the well-being of the colony. The plant origin of Propolis determines its chemical diversity. Bee glue's chemical composi- tion and quantity of the actives depends on the specificity of the local flora at the site of collection and thus on the geographic and seasonal weather characteristics of this site. Even if Propolis samples analysed from many different parts of the world (and with different chemical composition) have demonstra- ted they are active to the same indica- tions but with differences related to the chemical composition, Propolis diversity is quite a challenge to its use as nutra- ceutical and pharmaceutical ingredient. The plant origin of Propolis determines its chemical diversity. Bee glue's chemi- cal composition and quantity of the actives depends on the specificity of the local flora at the site of collection and thus on the geographic and seasonal weather characteristics of this site. Even if Propolis samples analyzed from many different parts of the world (and with different chemical composition) have demonstrated they are active to the same indications but with differences related to the chemical composition, propolis diversity is quite a challenge to its use as nutraceutical and pharmaceu- tical ingredient. AN ANCIENT FOLK REMEDY Propolis is one of the few natural reme- dies that has maintained its popularity over a long period of time. In fact, it has been used as a folk medicine since 300 b.C., leveraging its multiple proper- ties and activities. The pharmacologically active molecules in the Propolis are polyphenols: dewaxed raw Propolis contains more than 50% and more than 300 different polyphenols in different Propolis. These components have multi- ple effects on bacteria, fungi and viruses. In addition, Propolis and its components have anti-inflammatory and immuno- modulatory activities.
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  11. 11. ...TO M.E.D.® PROPOLIS SELECTION OF RAW MATERIAL CHARACTERIZATION AND STANDARDIZATION TYPE SELECTION - In order to obtain maximum raw material homogeni- za-tion, B Natural chooses its suppliers in temperate zones around the 45th parallel, where is well docu- mented that the main source of bee glue is the resinous exudate of the buds of Salica- ceae family plants, in particular of Populus genus. The so-called brown Propolis produced in these areas contains, within the others, a large quantity of ‘poplar bud’ pheno- lics: flavonoid aglycones (flavones and flavanones), phenolic acids and their esters. QUALITY SELECTION - B Natural guarantees the use of raw materials coming from strict control led origins. Each supplier signs a Technical Agree- ment that lists all the mandatory characteristics for the bee farming (good beekeeping practices) and for the raw material to be compliant with B Natural requirements. M.E.D.® Propolis extracts contain an intact, complete, identified and characterized polyphenolic complex. M.E.D.® Propolis is characterized and standardized by the presence of a biolo- gically active polyphenol complex, iden- tified in six major polyphenols: galangin, chrysin, pinocembrin, apigenin, pino- banksin, quercetin, having a relative concentration in the extract always grea- ter than 25% (w/w) determined in HPLC-ESI-MS. M.E.D.® Propolis standardized extracts can be used to obtain solid or liquid extracts containing always the same complex, with the same activity. This makes them easily adapting to any formulation and pharmaceutical forms, and totally reliable in research activities looking for dose/effect ratio. EXTRACTION OF ALL THE POWER The classic extraction of Propolis consists in the dissolution of raw Propolis in 80% alcohol content which extracts 75-80% of the polyphenols but however, melt unin- teresting resins and waxes too. Thanks to Dynamic Multi Extraction patented method, M.E.D.® Propolis extracts preserve the total of active molecules present in raw Propolis, not only the flavonoids but also phenolic acids. Moreover, Dynamic Multi Extraction allows to obtain the glycosyla- ted fraction of polyphenols in dry extracts microencapsulated in Arabic gum useful to increase the hydro dispersion. M.E.D.® Propolis extracts are pure from inactive resins and rich in polyphenols.
  12. 12. THE PATENTED METHOD Patent number: 001425516 THE WONDERFUL 6! M.E.D. PROPOLIS The patented method of extraction (Dynamic Multi Extraction M.E.D.®) modulates ethanol/water ratio during the manufacturing process. ACTIVES IDENTIFICATION The constant amount and fingerprint of M.E.D.® Propolis is guaranteed by a multi analytical approach (HPLC-MS,HPLC-UV, Spectophotometer). REPEATABILITY OF THE EXTRACTS The strict selection of propolis raw mate- rial, the oathented extraction method and the expertise of B Natural in polyphenols allow to identify by HPLC a fingerprint proper of brown propolis. TESTED EFFECTIVENESS In vitro and in vivo studies have proven M.E.D.® Propolis antioxidant, anti-inflam- matory and antimicrobial activity in many applications. The complex activity, linked to characterized polyphenols content, is repeatable in all M.E.D.® Propolis extracts . This table describes the antioxidant activity. Propolis Finished Products Polyphenols Content Microg Trolox/mg Polyphenols mg/ml ESIT12 (w/w) Oily extract(w/v) Glycolic extract(w/v) Glyceric extract(w/v) Hydroalcoholic Characterized Polyphenols Complex 16.5+0,8% 24.0+1,4% 81.2+3,7% 26.2+1,6% 69.7+2,0% 74.0+4,2% 79.4+4,2% 71.7+3,5% 74.4+3,8% 76.0+4,1% x105 6.0 5.0 4.0 3.0 2.0 1.0 0.0 20.00 1 234 5 6 7 89 10 11 12 13 15 18 19 21 20 24 22 25 27 29 2826 40.00 60.00 80.00 (min) µV HPLC-ESI-MS vs Poplar Brown Propolis standard HPLC-ESI-MS vs GAL of which: Quercetin (%) of which: Apigenin (%) of which: Pinobanksin (%) of which: Crisin (%) of which: Pinocembrin (%) of which: Galangin (%) TOTAL % HPLC-ESI-MS COMPLEX vs GAL 68,8 52,9 2,4 2,5 3,0 6,3 6,0 7,6 27,8 14,7 94,5 5,5 Aglycones (%) Glycones (%)
  13. 13. PROPOLIS M.E.D.® Different extracts same active complex Hydrogliceric extract Powders microincapsulated in Arabic gum Hydroalcoholic extract Oily Extract RAW PROPOLIS QA INSPECTION DEPURATION GREIT® ESIT® EPE® POLE® M.E.D.® mother extract Separation From Beewaxes DYNAMIC MULTI EXTRACTION (M.E.D.® ) ONLY THE GOOD OF PROPOLIS Polyphenols are a family of about 5000 molecules widely present in the plant world. Amongst Polyphenols, there are “sub-families” which differ from each other because of the number and characteristics of the phenolic units. These structures have an influence on the chemical and physical properties amongst which solubility. Standard method of extraction of Polyphenols from Propolis involve the use of a single solvent. The result is a partial extraction. The Dynamic Multi Extraction (M.E.D.®), after an HPLC analysis of the starting raw material, uses various concentrations of solvent during the whole production process thus extracting Polyphenols with different solubility and especially flavo- noids, phenolic acids and glycosilated fractions, separating them from those resins that can reduce their activity. The strength of our products is given by the richness and quality of bioacti- ves.
  14. 14. 16 Purify the polyphenols present in Propolis from beeswaxes and inert resins Obtain the first Propolis extracts standardized in total polyphenols in which six of them represent more than 25% Obtain extracts characterized and highly purified Guarantee the integral extraction of actives POLYPHENOLS PHENOLIC ACID STILBENS FLAVONOIDS FLAVONOLS FLAVANONESANTOCYANISFLAVANOLS APIGENIN CHRYSIN GALANGIN PINOCEMBRIN PINOBANKSIN QUERCETIN LIGNANS OTHERS OTHERS
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  16. 16. RESPIRATORY HEALTH The upper respiratory system includes the nose, nasal cavity, pharynx, and larynx with subglottic area of trachea. Upper respiratory tract infection (URTI) is a nonspecific term used to describe acute infections involving the nose, para- nasal sinuses, pharynx and larynx which serve as gateways to the trachea, bron- chi, and pulmonary alveolar spaces; of them rhinitis and sinusitis usually coexist and are concurrent in most individuals; thus, the correct terminology is now rhinosinusitis. Bee products have been used empirically for centuries especially for the treatment of respiratory diseases. Propolis can significantly reduce the number and severity of nighttime asthma attacks, improve pulmonary function and reduce inflammation. The aim of in vitro studies conducted using characterized polyphenols com- plex of M.E.D.® Propolis were to examine its activityagainst a large panel of micro- organisms involved in upper respiratory tract infections such as Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Moraxella catarrhalis, Haemophilius influenzae. In these tests were also investigated the activity of Characterized Polyphenols Complex against methicillin-sensitive (MSSA) and methicillin-resistant (MRSA) Staphylococcus aureus and Streptococ- cus pneumoniae Penicillinsusceptible, (Pen-s), Clindamycin-Erithromicyn resi- stant (Cli-Eri-R) and Macrolide-Erithromi- cyn resistant [Mef(E)+] clinical isolates. In addition, an in vitro study showed activity on bacterical biofilm formed by Staphylococcus aureus parcticularly difficult to eradicate. In particular, the antibacterial activity of Propolis M.E.D.® and it’s proved anti-inflammatory and antioxidant suggest using the characte- rized polyphenol complex to treat the URTI.
  17. 17. 19 Strain Staphylococcus aureus GISA MSSA L3797 96 24 48 96 96 48 12 6 3 Staphylococcus aureusATCC Staphylococcus aureus GISA MRSA Staphylococcus aureus MRSA macrolide-resistant Staphylococcus aureus MRSA Staphylococcus aureus MSSA ATCC 25923 Streptococcus pyogenes Streptococcus pneumoniae clindamycin/erithromycin resistant Streptococcus pneumoniae penicillin-resistant Moraxella catarrhalis Haemophilus influenzae ATCC 6538P L3798 ND060411 L4064 L1280 L49 L1542 6Streptococcus pneumoniae macrolide/erithromycin resistant L1402 L3917 > 750L1367 6L3292 75051907 Pseudomonas aeruginosa ATCC 27853 Code MIC (µg/ml) of polyphenols Minimum Inihibitory Concentration of Propolis M.E.D. characterized polyphenol complex
  18. 18. 20 For more info and all studies ask for Monographies
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  20. 20. INTIMATE CARE Genitourinary tract infections, caused by bacteria, protozoa and less frequently by fungus (Candida spp.) or virus, represent one of the most frequent diseases in daily clinical practice. Vaginal infections are so prevalent that they represent the most frequent reason for women to seek medical consultation. Several surveys have estimated that more than 70% of adult women have had a vaginal problems and have used vaginal products to treat infections. They are characterized by a large increase in the number of endogenous bacteria, typical- ly Gardnerella vaginalis and other pathogenic microorganisms such as Atopobium vaginae, Bacteroides spp., Prevotella spp., Peptostreptococcus spp., Mobiluncus spp., and Mycoplasma hominis and Candida spp. The last one affects approximately 75% of women of reproductive age and is the second most common cause of vaginitis. Furthermo- re, it has been reported that 40–50% of women will experience a recurrence, and 5–8% of adult women will develop recur- rent VVC, as defined by the occurrence of four or more episodes within 1 year. Urinary Tract Infections (UTIs) are the most common bacterial infections; it has been estimated that about 150 million people worldwide develop UTI each year. Effective methods to prevent UTI have been sought for many years, particularly since the beginning of the century due to the diffusion of multidrug resistance. One strategy to prevent urinary tract infections is the use of natural products such as cranberry (Vaccinium macrocar- pon) and Propolis. The activity of Characterized Polyphenols Complex of M.E.D.® Propolis was investi- gated against a large panel of microbial species that affect genitourinary tract, particularly women’s intimate area. The in-vitro studies were performed against vaginal fungi that arise in case of lowe- ring immune-system and contribute to vaginal dysbiosis such as C.albicans, C.parapsilosis, C.kruzei, C.guillermondi. The data obtained indicate a potential useful role of Characterized Polyphenols Complex of M.E.D.® Propolis in vaginal infectional treatments and suggest a more in-delpth clinical evaluation. The inactivity of Characterized Polyphenols Complex of M.E.D.® Propolis against Lactobacilli spp, that constitute the shield of vaginal mucosae, suggests an interesting collaboration between Characterized Polyphenols Complex of M.E.D.® Propolis and Lactobacilli spp. to improve vaginal immunity system.
  21. 21. 23 Strain Escherichia coli hyperpermeable G1640 47 Lactobacillus gasseri Lactobacillus acidophilus Neisseria gonorrhoeae ND787 ND786 L1600 Resistant 24 Gardnerella vaginalis L1629 47 Gardnerella vaginalis L1622 47 Resistant Neisseria gonorrhoeae L1601 24 Gardnerella vaginalis L1630 47 47 22 190 190 190 190 380 380 750 380 750 24 Escherichia coli L4242 Streptococcus pneumoniae penicillin-susceptible L44 Candida albicans ATCC 24443 L4120 Candida albicans ATCC 90028 L3023 Candida gullermondii ATCC6260 L2065 Candida parapsilosis ATCC22019 L4119 Candida parapsilosis ATCC 90018 L3022 L2280Candida kruzei L1011 L1365 L4013 ND736 Bacteroides fragilis ATCC 25285 Clostridium difficile Clostridium difficile ATCC 17858 Atopobium vaginae Code MIC (µg/ml) MIC values of Propolis MED extract are reported for the microorganism species tested.
  22. 22. 24 For more info and all studies ask for Monographies
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  24. 24. ORAL CAVITY Oral infections are one of the most common pathologies in general popula- tion, mostly due to complications of infections associated with poor oral hygiene. Infections cause pain, swelling and inflammations. Many types of infections can affect the oral cavity. The conditions, in acute or chronic form, can be related to specific areas of the mouth: if infections hit the gums, for example, they cause a gingivitis, if they involve the tongue we have a glossitis; if it causes edema of part or of all the palat mucosa, they can be generally defined as stoma- titis or mucositis. As a consequence of such inflammations, we could have secondary infections such as plaque and caries. A recently published systematic review on Propolis for oral health repor- ted that it can reduce oral infection and dental plaque and treat stomatitis. Exter- nal uses of Propolis include the use of pharmaceutical, cosmetic, and oral products such as ointment, gel, and mouthwash. Propolis when used as an ingredient in mouthwashes showed protection against oral disease which is likely due to its antimicrobial efficacy. In 2012 an observational study using M.E.D.® Propolis extract as ingredient in a chewingum to treat oral cavity inflam- mations has been performed by Clinica Dentale of Quartesolo (VI). Each of the 50 volunteers chewed chewingum contai- ning 3 mg of Polyphenols of M.E.D.® Propolis, two applications per day. 5 days after starting of treatment the 83% repor- ted a decrease of the symptoms linked to oral cavity inflammation; they observed also an amelioration of the aphthous (100%). Thanks to the results of these preliminary study, at the beginning of 2019 a clinical trial with M.E.D.® Propolis has been started to evaluate the activity on gingi- vitis compared to clorexidin performed by Italian Apitherapy Society.
  25. 25. 27 Chewing-gum base with Propolis M.E.D.® An observation analysis of activity of chewing-gum base with Propolis M.E.D.® extract (Data based on submit- ted questionnaire to 40 volunteers at private dentistry). Gum bleeding reduction Aphtous stomatitis redution Gum burning reduction Halitosis reduction
  26. 26. 28 For more info and all studies ask for Monographies
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  28. 28. The  gastrointestinal tract  consists of a hollow muscular tube starting from the oral cavity, where food enters the mouth, continuing through the pharynx, esophagus, stomach and intestines to the rectum and anus, where food is expelled. There are various  accessory organs that assist the tract by secreting enzymes to help break down food into its component nutrients. In the stomach Helicobacter pylori is the most important causative agent of chronic gastritis, also known as “nonspe- cific” and it is responsible for more than 90-95% of duodenal ulcers, too. Natural products are considered as attractive sources for new antiulcer treatments. Among them, Propolis has been used in folk medicine to treat gastric ulcer and this has boosted research in order to investigate and validate its use as an antiulcer agent. In particular, M.E.D.® Propolis thanks to its activity of on Helicobacter pylori , could be used to treat recurrences forms or as comple- mentary therapy with antibiotics drugs. The intestine is an important defence barrier for the body. The gastrointestinal tract (GI) gathers 2/3 of the overall immune system thanks to a complex microbiological ecosystem. The most important effects of GI microflora are to protect the host from infections and pathogens development, promoting the establishment and the activity of immu- nological tissues. It is very important to maintain intestinal ecosystem or to restore the balance after an antibiotic therapy. The proved anti-inflammatory, antioxidant and antibacterial activity of M.E.D.® linked with the no activity against Lactobacillus species suggest to use the characterized polyphenol com- plex in gastrointestinal care for dysbiosis events. GASTROINTESTINAL TRACT
  29. 29. 31 Strain Staphylococcus aureus MSSA ATCC 25923 L1280 48 48 6 6 Staphylococcus epidermidis ATCC 12228 L147 Streptococcus sanguis L1721 190Candida albicans ATCC 24443 L4120 380Candida parapsilosis ATCC 90018 L3022 750Bacteroides fragilis ATCC 25285 L1011 750Clostridium difficile ATCC 17858 L4013 ResistantLactobacillus acidophilus ND786 380Candida guillermondii ATCC 6260 L2065 380Clostridium perfringens ATCC 13124 L3697 750Escherichia coli ATCC 25922 L1281 750Clostridium difficile L1366 ResistantLactobacillus plantarum L19 48Bacillus cereus ATCC 10702 L85 750Clostridium perfringens HSR L4053 Streptococcus mutans ATCC 27351 48 Escherichia coli hyperpermeable G1640 190 Candida albicans ATCC 90028 L3023 380Candida kruzei L2280 380Clostridium difficile L1365 ResistantLactobacillus gasseri ND787 48Escherichia coli L4242 190 Candida parapsilosis ATCC 22019 L4119 190Listeria monocytogenes ATCC 13932 L1450 750Escherichia coli L47 ResistantLactobacillus paracasei L1693 Code MIC (µg/ml) polyphenols MIC values of Propolis MED extract are reported for the microorganism species test
  30. 30. 32 Active Polyphenols Compound from purified Propolis source
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  32. 32. SKIN CARE lin-resistant (MRSA) which is resistant to certain antibiotics that are commonly used for Staphylococcus spp. The studies carried out on M.E.D.® Propo- lis extracts have highlighted the in vitro anti-bacterial activity against Staphilo- cocci strains from ATTC library and clini- cal isolates both antibiotic resistant and susceptible. The activity of Characterized Polyphenols Complex was tested also against S. capitis, S. xylosus, S. simulans, S. haemolyticus, S. epidermidis showing an efficent activity against these species that affect and cause skin disorders. All skin diseases share the alteration of oxidative and inflammatory response. For these reasons, thanks its proved antioxidant, anti-inflammatory and antibacterial activity, linked to a wide range of formulation, M.E.D.® extracts are a new ingredient to use in cosmetic product as active ingredient or natural preservative. Skin disorders are among the most frequent, sometimes a little insidious but often very annoying conditions. Infections, stress, exaggerated UV ray exposure, pollutants can lead to the appearance of serious or light cutaneous manifestations. The human skin is cove- red by several bacterial populations that change according to the density and composition of the anatomical region. Some bacteria live on normal skin and cause no harm, some bacteria invade normal skin, break it and cause infections. Skin diseases can be caused by viruses, bacteria, fungi and parasites. The most common skin bacterial patho- gens are Staphylococci while Herpes simplex is most common viral skin agernt. Staphylococci bacteria can cause a wide range of infections. There are many types of Staphylococci but most infections are caused by a group called Staphylococcus aureus. This group inclu- des Staphylococcus aureus methicil- For more info and all studies ask for Flavocare® documentations
  33. 33. PRODUCT LIST
  34. 34. Solid Extracts Commercial name Titration Form Indications Special Characteritics Powder microencapsulated in Arabic Powder microencapsulated in Arabic gum Solid form such as capsules, tablets (also orosoluble and effervescent), candies, chewing gum, granulates, soluble powders in cans or sachets Solid form such as capsules, tablets (also orosoluble and effervescent), candies, chewing gum, granulates, soluble powders in cans or sachets Solid form such as capsules, tablets (also orosoluble and effervescent), candies, chewing gum, granulates, soluble powders in cans or sachets Active Ingredients Solid Extracts Commercial name Titration Form Indications Special Characteristics Powder microencapsulated in Arabic gum Powder microencapsulated in Arabic gum Conventional powder (Maltodextrin) Traditional solid form such as capsules, tablets to be swallowed, candies, chewing gum and granulates Active Ingredients Extracts, purified extracts and mainly mono-compound extracts. The group includes conventional production and special production. The Propolis extracts are made under M.E.D.® process method. Organic version also available Organic version also available Organic version also available Organic version also available Made with Brazilian raw Propolis Solid form such as capsules, tablets (also orosoluble and effervescent), candies, chewing gum, granulates, soluble powders in cans or sachets Powder microencapsulated in Arabic gum Propolis ESIT® 12 Propolis ESIT® 6 Propolis ESIT® 3 Propolis GREENART Propolis ESM 8-12% Tot. Polyphenols expressed as Galangin UV folin Ciocalteu HPLC ESI MS >2.5%Tot. Polyphenols expressed as galangin UV folin Ciocalteu > 12% Tot. Polyphenols expressed as Galangin UV folin Ciocalteu HPLC ESI MS > 3% Tot. Polyphenols expressed as Gala ngin UV folin Ciocalteu HPLC ESI MS > 2.5% Tot. Phenolic acid vs. Artepillin C (HPLC-UV) of wich Artepillin C >10% > 5% Tot. Phenolic acid vs. Artepillin C (HPLC-UV) of wich Artepillin C >10% > 6% Tot. Polyphenols expressed as Galangin UV folin Ciocalteu HPLC ESI MS
  35. 35. Liquid extracts Commercial name Titration Form Indications Special Characteristics Propolis GREIT® 120 > 12 mg/ml Tot. Polyphenols HPLC ESI MS 6 mg/ml Tot. Polyphenols HPLC ESI MS > 10 mg/ml Tot. Phenolic acids vs Artepilin C (HPLC - UV) Presence of Artepillin C E : D = 20 : 1 20% natural propolis Liquid: water and glycerol Liquid: water and glycerol Liquid: water and glycerol Liquid: water and glycerol Liquid: water and glycol propilene Avaible in forms: - Hydroglyceric - Oil Dense liquid: oil Syrup and aqueous based liquid products (drops, sprays, syrups.) with antibiotic, antibacterical, antioxideant, immunostimulant properties Syrup and aqueous based liquid products (drops, sprays, syrups.) with antibiotic, antibacterical, antioxideant, immunostimulant properties Syrup and aqueous based liquid products (drops, sprays, syrups.) with antibiotic, antibacterical, antioxideant, immunostimulant properties Syrup and aqueous based liquid products (drops, sprays, syrups.) with antibiotic, antibacterical, antioxideant, immunostimulant properties Cosmetics and dermocosmetics in liquid form such as ointments, fluid and dense, liquid detergents in particular for the intimate hygene. Activities: antibacterical, healing, fungicide, anaesthetic Cosmetics and dermocosmetics in liquid form such as ointments, fluid and dense, liquid detergents in particular for the intimate hygene. Activities: antibacterical, healing, fungicide, anaesthetic Fatty base cosmetic products: lipsalves, stick, pearls... with fungicide and antibacterical properties Propolis GREIT® 60 Propolis GREENART Propolis Hydroglyceric EXT. - Grei C Propolis Glycolic EXT. Propolis Pole 300 Flavocare > 30 mg/ml Tot. Polyphenols HPLC ESI MS Various tritration 38 Organic version also available Ethanol free Organic version also available Ethanol free Organic version also available Organic version also available Organic version also available Ethanol free Made with Brazilian raw Propolis Organic version also available
  36. 36. Liquid alcoholic and syrup Commercial name Titration Form Indications Special Characteristics Propolis EPE® 50 (70GA) > 50 mg/ml Tot. Polyphenols expressed as Galangin UV Folin Ciocalteu 38 mg/ml Tot. Polyphenols expressed as Galangin UV folin Ciocalteu HPLC ESI MS Liquid alcoholic Liquid alcoholic Liquid alcoholic Liquid alcoholic Semifluid paste Syrup Syrup Liquid, alcoholic, hydroalcoholic forms, drinks, drops and sprays with antibacterical, antiflu, healing, anaesthetic properties. Specific cosmetics and dermocosmetics with antibacterical, healing and anaesthetic action Liquid, alcoholic, hydroalcoholic forms, drinks, drops and sprays with antibacterical, antiflu, healing, anaesthetic properties. Specific cosmetics and dermocosmetics with antibacterical, healing and anaesthetic action Liquid, alcoholic, hydroalcoholic forms, drinks, drops and sprays with antibacterical, antiflu, healing, anaesthetic properties. Specific cosmetics and dermocosmetics with antibacterical, healing and anaesthetic action Fluid, dense and solid food products (candies, syrups, honey...). Realization of liquid fluids for simple dilution (alcoholics) with antibiotic, antibacterical, antioxydant, immunostimulant activities Fluid, dense and solid food products (candies, syrups, honey...). Realization of liquid fluids for simple dilution (alcoholics) with antibiotic, antibacterical, antioxydant, immunostimulant activities Propolis EPE® 38 (80GA) Propolis Hydroalcoholic (EXT. 80GA) Propolis soft EXT. concentrated Propolis syrupy EXT. Propolis syrupy EXT. 8 - 12% Tot. Polyphenols expressed as Galangin UV folin Ciocalteu > 1% Tot. Polyphenols expressed as Galangin UV folin Ciocalteu Semifluid paste Propolis soft EXT. (30GA) > 12% Tot. Polyphenols expressed as Galagin UV folin Ciocalteu HPLC ESI MS > 6% w/w natural propolis For agricultural purpose E : D = 10 : 1 Min 20 mg/ml Tot. Polyphenols HPLC ESI MS Propolis EPE® 20 (60GA) Organic version also available Product on demand 39
  37. 37. Synergy Line DescriptionCommercial name Form Indications Special Characteristics PROPOLFENOL® PROPOLSAVE® A fitocomplex richer in active principles and more functioning because it uses the synergy between Propolis polyphenols, catechins and OPCs Propolis M.E.D. ® Characterised Polyphenols Complex with Polysaccharides from Opunxia™ Powder microencapsulated in Arabic gum Powder microencapsulated in Arabic gum Anti-inflammatory, antioxidant, antibacterical, bacteriostatic, antimycotic and antiviral properties Anti-inflammatory, mucoprotective and antibacterical properties. Applications: tablets, capsules, fizzy tablets Synergistic extract B Natural has developed some synergistic extracts with the purpo- se to obtain new products with certified and proven activity. The production process applied is the same innovative and patented technology M.E.D.® (Dynamic Multi Extraction). Through the choice and combination of different highly functional raw materials, this procedure allows to accompli- sh a multi-action combined products. These synergic extracts are unique solutions ready to be used, able to elicit multiple effects and perfect to enhance old and new formulations. 40
  38. 38. Barros et al., 2008. Evalution of antiulcer activity of the main phenolic acids found in Brazilian green propolis. Journal of Ethnopharmacology. 120, 372-377. Boyanova et al., 2003 . Inhibition of Helicobacter pylori growth in vitro by Bulgarian propolis: preliminary report. Journal of Medical Microbiology. 52:417-9. Carvalho et al., 2015. Propolis: a complex natural product with a plethora of biological activities that can be explored for drug developmen Evidence-Based Complementary and Alternative Medicine. 29: 206439. Coutaux et al., 2005. Hyperalgesia and allodynia: peripheral mechanisms. Joint Bone Spine 72, 359-371. Curti V, Zaccaria V, Tsetegho Sokeng AJ, Dacrema M, Masiello I, Mascaro A, D'Antona G, Daglia. 2019. Bioavailability and In Vivo Antioxidant Activity of a Standardized Polyphenol Mixture Extracted from Brown Propolis. Internationa Jornal of Molecular Sciences 5, 12-20 Galeotti F, Crimaldi L, Maccari F, Zaccaria V, Fachini A, Volpi N. 2017 Selective treatment to reduce contamina- tion of propolis by polycyclic aromatic hydrocarbons (PAHs) still preserving its active polyphenol component and antioxidant activity. Natural Product Research 17, 1971-1980. Galeotti F, Maccari F, Fachini A, Volpi N. 2018 Chemical Composition and Antioxidant Activity of Propolis Prepared in Different Forms and in Different Solvents Useful for Finished Products. Foods 3, 7-19 Imhof et al., 2005. Propolis solution for the treatment of chronic vaginitis. International Journal of Gynecology and Obstetrics. Kathleen et al., 2014. Propolis decreases lipopolysaccha- ride induced inflammatory mediators in pulp cells and osteoclasts. Dental Traumatology. 30: 362-367. Krol et al., 2013. Propolis: Properties, Application, and Its Potential Evidence-Based Complementary and Alternative Medicine Lavigne et al., 2011. Propolis can potentialise the anti-adhesion activity of proanthocyanidins on uropathogenic Escherichia coli in the prevention of recurrent urinary tract infections. Biomedical Central Research Notes 4:522. Martin et al., 1998. Anti-oxidant mechanisms involved in gastroprotective effects of quercetin. Zeistschrift fur Naturforschung C 52, 82-88. Martins et al., 2012. Brazilian green propolis: anti-inflam- matory property by an immunomodulatory activity. Evidence Based Complement Alternative Medicine. Miroslava et al., 2012. In vitro and In vivo antimicrobial activity of propolis on the microbiota from gastrointe- stinal tract of chickens. Journal of Environmental Science and Health. Part A 47,1665-1671. Ota et al., 2001. Antifungal activity of propolis on Different species of Candida. Mycoses 44, 375-378 Ramanauskienè et al., 2011. Propolis oil extract: quality analysis and evaluation of its antimicrobial activity. Natural Product Reports 25, 1463-1468 Sforcin et al., 2011. Propolis: Is There a Potential for the Development of New Drugs? The Journal of Ethnopharmacology. 133 253-60 Shinmei et al., 2009. Effects of Brazilian propolis on sneezing and nasal rubbing in experimental allergic rhinitis of mice. International Immunopharmacology. 31, 688-693. Volpi N, Bergonzini G. 2006 Analysis of flavonoids from propolis by on-line HPLC-electrospray mass spectro- metry. Journal of Pharmaceutical and Biomedical Analysis 42, 354-361. Volpi N, Fachini A. Procedimento Per L’ottenimento di Estratti Integrali di Propoli Ricchi in Polifenoli e Dotati di Attività Antibatterica e Sua Applicazione Nella Prevenzione e Trattamento di Processi Infettivi di Origine Batterica. Ufficio Italiano Brevetti e Marchi No. 0001425516, 02/02/2017. Vynograd et al., 2000. A comparative multi-centre study of the efficacy of propolis, acyclovir and placebo in the treatment of genital herpes (HSV). Phytomedici- ne. Zaccaria, V.; Curti, V.; Di Lorenzo, A.; Baldi, A.; Maccario, C.; Sommatis, S.; Mocchi, R.; Daglia, M. 2017 Effect of green and brown propolis extracts on the expression levels of microRNAs, mRNAs and proteins, related to oxidative stress and inflammation. Nutrients, 10, 1090 Zedan et al., 2009. Propolis as an alternative treatment for cutaneous warts. International Journal of Dermato- logy, 48, 1246-1249 BIBLIOGRAPHY Scientific studies available upon request.
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  40. 40. PROPOLIS AND BEEKEEPING DERIVATIVES B Natural srl Via Gran Sasso, 33 20011 Corbetta (Milan), Italy T. +39 02 49470332 F. +39 02 87152774 bnatural@bnatural.it www.bnatural.it

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