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Lab 10 Direct Measurement Of VO2


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Lab 10 Direct Measurement Of VO2

  1. 2. Materials Used <ul><li>Treadmill </li></ul><ul><li>Metabolic cart </li></ul><ul><li>HR monitor </li></ul><ul><li>Head gear with mouthpiece </li></ul>
  2. 3. Methods <ul><li>Warm-up for 5-10 mins </li></ul><ul><li>Place HR monitor and headgear with mouthpiece on the subject with a nose clip!! </li></ul><ul><li>Begin the subject at a comfortable run that’s not too challenging because every 2 minutes the grade will be increasing. Your lab instructor will give you more information on the protocol </li></ul><ul><li>Need HR, VO 2 (absolute and relative) every minute and RPE every 2 minutes (end of the stage) </li></ul>
  3. 4. Methods
  4. 5. How to set up a mouthpiece These are all of the pieces needed to construct the mouthpiece
  5. 6. How to set up a mouthpiece
  6. 7. How to set up a mouthpiece Using soap helps the green mouthpiece go on easier
  7. 8. Completed headgear with mouthpiece Whenever using a mouthpiece you MUST wear a nose clip! To disassemble: take mouthpiece apart, rinse all parts with warm water and then place in CIDEX solution
  8. 9. How to Calibrate a Metabolic Cart Choose flowmeter calibration Put temperature, relative humidity and barometric pressure in proper boxes * Once finished with flowmeter, do gas calibration and follow instruction, you only need to open gas valve on the back side of the cart
  9. 10. How to Calibrate a Metabolic Cart Put a mouthpiece together but instead of the green mouth part but a calibration part on instead. Place calibration syringe on the mouthpiece (as shown) 
  10. 11. How to Calibrate a Metabolic Cart This screen will come up once it is ready to calibrate, you will need to perform 4 flushes which is just pulling the syringe in and out in a uniform manner. Hold the mouthpiece and calibration syringe like pictured below
  11. 12. How to Calibrate a Metabolic Cart Once you have finished the flushes, do the same thing but now it will calibrate the number on the screen should be between 2.900-3.100 (higher end) in order to obtain a good calibration This screen will appear once the calibration is complete. The average needs to be +/- 3%