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Payload, Operation & Science Services including Vapor-Diffusion and Counter-Diffusion Cell Capabilities

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Payload, Operation & Science Services including Vapor-Diffusion and Counter-Diffusion Cell Capabilities

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Payload, Operation & Science Services including Vapor-Diffusion and Counter-Diffusion Cell Capabilities

  1. 1. TBE & NCP Proprietary 1 • Who we are…  The TBE + NCP team have a long and successful Space Shuttle, Russian-Mir and ISS flight history with Protein Crystal Growth hardware and research.  Tim Allen, Space Systems Chief Engineer, has worked at Teledyne for over 30 years and has integrated and operated 15 Protein Crystal Growth missions on the Russian-Mir, Shuttle, and ISS.  Dr. Daniel Carter, President and CEO of NCP, has over 30 years experience in Protein Crystal Growth microgravity payloads as both a flight investigator and hardware developer. He was the Principal Investigator for 12 Protein Crystal Growth payloads on the Shuttle, Russian-Mir, and ISS. Dr. Carter’s highly successful and unique professional experience includes time spent as a NASA employee where he held positions as NASA Senior Scientist for Biophysics and Chief of the Biophysics and Advanced Materials Branch. Through his post-NASA career, he founded and guided NCP, which encompassed the determination of hundreds of atomic structures of proteins utilizing various synchrotron facilities around the world for the purposes of achieving safer, more effective pharmaceuticals. In addition to extensive experience in structural biology, his current molecular biology experience in the recombinant production of proteins is extensive and includes proteins recently recognized as standards of excellence by the National Institutes of Standards. Dr. Carter is a member of the NASA Science Development Advisory Panel for the Real Time Protein Crystal Growth (RTPCG). 2018 Microgravity Molecular Crystal Growth Workshop Hauptman-Woodward Medical Research Institute July 19, 2018
  2. 2. TBE & NCP Proprietary 2 • Protein Crystallization Apparatus for Microgravity (PCAM) flight history: STS-62, STS-63, STS-67, STS-73, STS-83, STS-85, STS-94, STS-95, STS-100/ISS 6A, STS-101, STS-108/ISS UF-1, STS-111/ISS UF-2, STS-112/ISS 9A 2018 Microgravity Molecular Crystal Growth Workshop Hauptman-Woodward Medical Research Institute July 19, 2018
  3. 3. TBE & NCP Proprietary 3 • Typical PCAM flight configuration in a refrigerator / incubator 2018 Microgravity Molecular Crystal Growth Workshop Hauptman-Woodward Medical Research Institute July 19, 2018
  4. 4. TBE & NCP Proprietary 4 2018 Microgravity Molecular Crystal Growth Workshop Hauptman-Woodward Medical Research Institute July 19, 2018 • Enhanced Diffusion-controlled Crystallization Apparatus for Microgravity (EDCAM) assembly consists of Counter Diffusion Cells (CDCs) and Generic Enclosure System (GES) • The predecessor to EDCAM was DCAM and its flight history consists of STS-73 & STS-113, and Russian Mir missions: STS-76, STS-79, STS-81, STS-84, STS-86, STS-89
  5. 5. TBE & NCP Proprietary 5 2018 Microgravity Molecular Crystal Growth Workshop Hauptman-Woodward Medical Research Institute July 19, 2018 • Typical EDCAM flight configuration in a refrigerator / incubator
  6. 6. TBE & NCP Proprietary 6 2018 Microgravity Molecular Crystal Growth Workshop Hauptman-Woodward Medical Research Institute July 19, 2018
  7. 7. TBE & NCP Proprietary 7 • CDC cut-away view 2018 Microgravity Molecular Crystal Growth Workshop Hauptman-Woodward Medical Research Institute July 19, 2018
  8. 8. TBE & NCP Proprietary 8 2018 Microgravity Molecular Crystal Growth Workshop Hauptman-Woodward Medical Research Institute July 19, 2018
  9. 9. TBE & NCP Proprietary 9 2018 Microgravity Molecular Crystal Growth Workshop Hauptman-Woodward Medical Research Institute July 19, 2018
  10. 10. TBE & NCP Proprietary 10 2018 Microgravity Molecular Crystal Growth Workshop Hauptman-Woodward Medical Research Institute July 19, 2018 Glucose Isomerase Ground Control in CDC Glucose Isomerase flight sample DCAM
  11. 11. TBE & NCP Proprietary 11 • Our objective is to reduce the complexity in the payload development and integration process, and to that end, the Teledyne and NCP team will function as an implementation partner to assist any Principle Investigator (PI) in their Microgravity Molecular Crystal Growth (MMCG) research. • Our team understands the unique challenges with MMCG research, integration and ops. Challenges include:  Transportation to and from launch site  Early development of ground procedures with contingency responses  Toxicological boundaries  Proteins that degrade in transit (i.e., precipitate)  Launch scrubs / delays  Hardware failures experienced during verification program  Ground Truth Testing Issues 2018 Microgravity Molecular Crystal Growth Workshop Hauptman-Woodward Medical Research Institute July 19, 2018 Our streamlined integration approach is a key first step toward simplifying payload integration, making it easier to fly payloads on the ISS, thereby increasing feasibility and interest for more research and commercial organizations to sponsor ISS payloads and take advantage of the ISS as a National Laboratory (NL) asset.
  12. 12. TBE & NCP Proprietary 12 Summary of services: • Sustained contact and orientation of the PI to the ISS mission integration and operations processes • Ground-based hardware and supporting materials / documentation • Development of crucial science requirements such as temperature, protein stability, and identification of any safety precautions to the loading team (e.g., animal or human derived materials) • Documentation detailing how to secure protein samples prior to launch & steps required to return unused samples • Coordinate with PI for post-flight distribution of samples & reserve facilities for post-mission sample analysis • Through sustained collaboration with PI identify the most probable crystallization conditions • Development of Mission Characteristics Summary (MCS) and Integration and Operations Agreement (IOA) to facilitate informed decisions and mission success  The MCS represents a collection of payload unique and mission-specific manifest data, such as environmental characteristics of the payload and launch vehicle to determine incompatibilities, launch date/time, and possible conditions, launch vehicle accommodations, transfer date/time to ISS, ISS accommodations, major mission milestones (docking, EVA, joint ops, etc.)  The IOA represents the payload, launch vehicle, CASIS and ISS interests regarding hardware integration, safety, and flight operations. It ensures the required support is provided to the payload and to minimize the potential risk for incomplete or erroneous understandings regarding support requirements and/or accommodations  Execute Pre-Shipping Activities  Execute Post-Shipping Activities at the launch site  Execute Post-Loading Activities at the launch site  Execute On-Orbit Operations  Execute Retrieval of Hardware/Samples/Crystals and Transport  Execute Requirements verification (safety, interface and science) and maintenance throughout the life of the project 2018 Microgravity Molecular Crystal Growth Workshop Hauptman-Woodward Medical Research Institute July 19, 2018

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