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pPICZo IgNAR+
4721 bp
Scyliorhinus canic
u
l
a
I
g
N
A
R
-
d
V
4
R
E
V
S
c
y
l
i
o
r
h
i
n
u
s
c
a
n
i
c
u
la
IgNAR-dV4 FWD
1/2
MetOH-induced monocopy secretion vector for transfection into Pichia pastoris.
Method for yeast-based production
of monoclonal shark IgNAR for pharma applications.
EcoRI-cut overhangs, Gibson-assembled primers.
FWD:
41bp,
41.46% GC,
Tm=65.79°C
REV:
40 bp
60.00% GC
Tm=73.07°C
2/2
spIkE E-1
6681 bp mediated production of SARS-CoV-2 SPIKE protein for use in variant-resilient
ELISA testing.
E. coli-

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plasmids

  • 1. pPICZo IgNAR+ 4721 bp Scyliorhinus canic u l a I g N A R - d V 4 R E V S c y l i o r h i n u s c a n i c u la IgNAR-dV4 FWD 1/2 MetOH-induced monocopy secretion vector for transfection into Pichia pastoris. Method for yeast-based production of monoclonal shark IgNAR for pharma applications. EcoRI-cut overhangs, Gibson-assembled primers. FWD: 41bp, 41.46% GC, Tm=65.79°C REV: 40 bp 60.00% GC Tm=73.07°C
  • 2. 2/2 spIkE E-1 6681 bp mediated production of SARS-CoV-2 SPIKE protein for use in variant-resilient ELISA testing. E. coli-