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MAKERCAMP PROPOSAL
B I O T E X T I L E S
TAUGHT AND CONCEIVED BY J. CELIS-RODRÍGUEZ
WEEK I
BACTERIAL MATERIALS
1
BY: J. CELIS-RODRÍGUEZ
3 SESSIONS
2.5 HRS/SESSION
SESSION 1
(CULTURING LEATHER ALTERNATIVES)
Campers will learn about leather’s outsized environmental impact, the currently
proposed alternatives, and a home-lab-friendly open-source method for the
manufacture of bacterial leather, culturing custom SCOBYs to fit their textile needs.
OVERVIEW:
Fast Fashion, sustainability, and the leather industry.
Textile alternatives and intro to further reading..
Bacterial culture practicum.
Next-session preview
—
SESSION 2
(CULTURE AND TREATMENT OF BACTERIAL MATERIAL)
Campers will learn how to propagate and continue their culture for long-term
production, how to modify the composition and material results of said culture, and
how to harvest, dry, and treat the resulting material for use in textile projects.
OVERVIEW:
Care, modification, and reproduction of textile culture.
Harvest and treatment of bacterial leather (tanning, starch strengthening, etc.).
Drying and surface patterns.
—
SESSION 3
(DESIGN AND MANUFACTURE OF BACTERIAL LEATHER PRODUCTS)
Campers will learn and apply design and material property considerations for
working with cultured material, and will deliver a final product of their own design
(with assistance).
OVERVIEW:
Basic leatherwork design and material considerations.
Cut, pattern, and sewing.
Alternative methods and experimentation (wet fusion, weaving, blend-&-foam, etc.)
2
BY: J. CELIS-RODRÍGUEZ
SESSION 1
MATERIALS PER CAMPER
● 1 spray bottle
● 1 plastic container >90cm2
● 1m2
of porous fabric.
● 1 xacto knife or similar implement.
● Hotplate.
● Pot or recipient to boil water in (preferably ceramic or glass)
● Wooden spoon.
● Latex gloves (+ latex-free alternatives)
● 100 mL 70% ethanol.
● 1L water.
● 50g red hibiscus flower, o 20g Camelia sinensis
● ⅓ bottle of commercial, unpasteurized kombucha.
● 200g white sugar.
● Water-based food coloring (optional).
—
SESSION 2
MATERIALS PER CAMPER
● Culture from previous session.
● Latex gloves (+ latex-free alternatives)
● Spray bottle of 70% ethanol.
● 120cm thin, untreated wood or MDF (~3mm).
● 4 clamps or 4 wood screws
● Drill or electric screwdriver (of screws were chosen for the previous point).
● 50mL vegetable oil.
● 1 smooth cloth.
● 1 xacto knife.
—
SESSION 3
MATERIALS PER CAMPER
● Sewing machine.
● Set of fabric-cutting rulers.
● 3 spools of cotton thread.
● Sewing needle (medium gage, steel).
● Xacto, fabric scissors, architect’s knife.
● Plotter paper and fabric pencil.
3
BY: J. CELIS-RODRÍGUEZ
WEEK II
PHYTOTEXTILES
4
BY: J. CELIS-RODRÍGUEZ
3 SESSIONS
2.5 HRS / SESSION
SESSION 1
(BASIC PHYTOMATERIALS)
Campers will become familiar with their human (and specifically Mexican) heritage
through the discovery and manufacture of vegetable fiber textiles through modern
techniques and technology applied to traditional methods.
OVERVIEW:
Human phytorelations and non-material value.
Methods for the transformation of found phytomaterials.
Modern applications for traditional methods.
Design and material considerations.
—
SESSION 2
(SECONDARY PHYTOMATERIALS [4H])
Focus on phytomaterials derived from non-fibrous matter, plant waste, and
chemical or biological reactions for the generation of zero-waste textiles.
OVERVIEW:
Textiles from juice and extracts.
Textiles from fruit waste.
Vegetable fiber reconstitution and biomaterial reinforcement.
—
SESSION 3
(PHYTOMATERIAL DESIGN & EXPERIMENTATION)
Campers will learn to conceptualize and realize projects employing a variety of
phytomaterials (incl. those developed in previous sessions, wood, MDF, cotton, etc.)
with a consideration for material properties and design language, playing with
texture, properties, and experimental production.
OVERVIEW:
Transversal phytomaterial considerations.
Design, cutting, and construction.
5
BY: J. CELIS-RODRÍGUEZ
SESSION 1
MATERIALS PER CAMPER
● Fallen branches, >1cm thick.
● Fallen leaves.
● Well-sharpened knife or blade.
● Manual whetstone.
● Sodium bicarbonate.
● 1L water
● Cooking pot.
● Hotplate.
● 50mL vegetable oil.
● 20mL glycerol.
—
SESSION 2
MATERIALS PER CAMPER
● 100g cornstarch.
● 50mL corn oil.
● 8 oranges
● 1 mesh sieve
● Pot
● Hotplate.
● Xacto knife.
● Whisk.
● Silicone spatula.
● Wax paper.
● 50mL vegetable oil
● 30mL glycerol.
● 1 fine metal grater
● 2g ground cinnamon
● 1 blender
● 10mL white vinegar
● 500mL water
● 1m2
cheesecloth
● 1 rolling pin
● 50g spent coffee grounds
● 10g sodium alginate
● 20g calcium chloride
6
BY: J. CELIS-RODRÍGUEZ
SESSION 3
MATERIALS PER CAMPER
● Materials from the previous sessions.
● Sewing machine.
● Plotting paper.
● Xacto and fabric scissors.
7
BY: J. CELIS-RODRÍGUEZ
WEEK III
MYCOMATERIALS
8
BY: J. CELIS-RODRÍGUEZ
4 SESSIONS
4 HRS / SESSION
SESSION 1
(MICRO-MYCO-LABORATORIES)
Campers will be introduced to the fungal kingdom, its ecological role, and its
potential for material innovation. They’ll learn and apply basic mycelial culture
techniques, build portable microlaboratories, and upkeep and reproduce their first
mycelial culture (Pleurotus spp.)
OVERVIEW:
Fungi.
Fungal materials and the future of textile innovation.
An introduction to mycelial culture.
Design and construction of portable laboratories.
Inoculum and mother cultures.
—
SESSION 2
(COMPOSITE, SELF-REPAIRING TISSUE)
Focus on the culture and care of composite mycelial textiles and living textile
management.
OVERVIEW:
Enclosure design and construction (bioreactors 101).
Culture inoculation, scaling, and treatment.
Composite material design and considerations.
Composite use and the maintenance of living material.
—
SESSION 3
(PURE MYCOTEXTILES + DESIGN PT. 1)
Campers will be introduced to pure mycotextiles and apply previous knowledge to
the construction of an air-feed bioreactor for the culture of pure mycelium for foam,
leather, and treated textile manufacture.
OVERVIEW:
Enclosure design and construction, pt. 2.
Maintenance of pure mycocultures.
Pure mycelium harvesting and treatment.
Material considerations, patterns, and sewing.
9
BY: J. CELIS-RODRÍGUEZ
SESSION 4
(DESIGN PT. 2)
Campers will familiarize themselves with material considerations relevant to their
cultured tissue, and will design and manufacture a final product of their own
design. They will also have an opportunity to learn of advanced mycotextile
techniques, including mycocircuitry and capacitive surfaces.
OVERVIEW:
Cut, patterns, and sewing, pt. 2.
Use of non-textile mycomaterials.
Finishing.
10
BY: J. CELIS-RODRÍGUEZ
SESSION 1
MATERIALS PER CAMPER
● 1 alcohol lamp.
● Large steel forceps.
● 1 collapsible plastic container, 20L
● 1 aluminum can
● Xacto knife.
● Latex gloves.
● 2 petri dishes.
● 1 Mason Jar (200mL minimum).
● 9cm2
1cm-thickness polyethylene foam (or non-toxic equivalent).
● 1 spray bottle
● Scalpel, surgical.
● Hotplate.
● 250mL Erlenmeyer flask.
● Non-polymer towel.
● 1 glue gun, silicone.
● 1 drill.
● 1 black sharpie.
● 2 medium russet potatoes.
● 1L water.
● 20g agar agar
● 100mL ethanol 70%
● 1 medium fresh Oyster mushroom( Pleurotus spp.).
—
SESSION 2
MATERIALS PER CAMPER
● Pot
● Hotplate
● Mason Jar (200mL minimum)
● 3mL intramuscular injection syringe.
● Latex gloves.
● Hermetic seal plastic container, rectangular (30×20cm minimum).
● Xacto knife.
● 1 alcohol lamp.
● Culture from last session.
● Portable lab from last session.
● 1L water.
● 1 texture (hay, cork, paper, fabric scraps, etc.)
● 4 acid-free coffee filters.
● 1 black sharpie.
11
BY: J. CELIS-RODRÍGUEZ
SESSION 3
MATERIALS PER CAMPER
● Hermetic seal plastic container, rectangular (30×20cm minimum).
● 3 30cm UV LED strips.
● 4 small-pore coffee filters
● 2 glass bottles or jars, hermetically sealable
● 2 large pieces of unpainted corrugated cardboard.
● 10cm flexible hose D=12mm
● 1m flexible hose Dint
.
=4mm
● 1 breadboard.
● ~30 breadboard pin cables
● 1 drill with 2mm metal-safe bit.
● Glue gun.
● Hotplate
● Pot.
● 3 spray bottle head
● 1 servo.
● 1 Arduino-compatible PC fan
● Arduino IDE (installed on laptop).
● 1 self-sealable injection port, D=1cm.
● 2 medium russet potato
● 1L water.
● 100mL 70% ethanol
● Electrical tape.
● Culture from session 4
● Sewing machine
● 3 spools of cotton thread.
● Embroidery needle. (medium gage, steel)
● Textile result from past session.
● Scissors.
● Plotter paper.
● Pencil.
—
SESSION 4
MATERIALS PER CAMPER
● Sewing machine
● 3 spools of cotton thread
● Embroidery needle (medium gage, steel).
● Xacto knife
● Scissors.
● Plotter paper.
12
BY: J. CELIS-RODRÍGUEZ
WEEK IV
CULTURED DYES
13
BY: J. CELIS-RODRÍGUEZ
3 SESSIONS
4 HRS / SESSION
SESSION 1
(VEGETABLE DYES)
Campers will learn about sourcing and the dye and color process, and will explore
our local ecosystem in order to ethically and sustainably select dye sources, and will
apply pigment extraction, alteration, and fixing techniques.
OVERVIEW:
Dye and extraction.
Ethical and local sourcing (w/ excursion).
Pretreatment, mordants, y fixing.
Liquid dye extraction.
Powdered dye extraction and printing.
Self-resupplying culture.
—
SESSIONS 2 Y 3
(LIVING MICROBIOLOGICAL DYES, PTs. 1 & 2)
Campers will learn to isolate and culture pigment-producing microorganisms and
live-dye textiles.
OVERVIEW:
Intro to microbiological dyes.
Handprint/soil cultures & organism selection.
ID techniques.
Isolation and monobiotic culture.
Live-dyeing.
Care and knowledge of your bacterial partner.
14
BY: J. CELIS-RODRÍGUEZ
SESSION 1
MATERIALS PER CAMPER
● Hotplate
● Pot.
● Wooden spoon.
● Knife.
● Cutting board.
● Scissors.
● 4L water.
● 30mL glycerol.
● At least 1m2
plant-based fabric (cotton, hemp, denim, bacterial leather, etc.) o
1 spool of yearn.
● 50mL white vinegar (3% AtAc)
● 10g alum.
● 100mL 70% ethanol.
● 1 plastic funnel.
● 2 coffee filters
● Alcohol lamp.
● Ash recipient.
● Request for leaves, branches, peels, etc. with interesting colors..
—
SESSION 2
MATERIALS PER CAMPER
● Hotplate.
● Pot.
● Wooden spoon.
● Lab spatula.
● Alcohol lamp.
● 1 Mason Jar, hermetically sealable.
● 3 petri dishes,.
● Xacto knife.
● 1L water.
● 30mL glycerol.
● 100mL 70% ethanol.
● 20g agar agar
● 20g white sugar.
● 20g pea protein.
15
BY: J. CELIS-RODRÍGUEZ
WEEK V
PHYCOSTRUCTURES
16
BY: J. CELIS-RODRÍGUEZ
4 SESSIONS
4 HRS / SESSION
SESSION 1
(PHYCOFIBERS, WOOL, AND CULTURE)
Campers will become familiar with microalgae, their material potential and growth
habits, and will build a DIY minibioreactor for the culture of Cladophora spp.
OVERVIEW:
Algae and their habits.
Liquid bioreactors: construction & design.
Custom culture media.
Microalgae inoculum and continuous culture.
—
SESSION 2
(PROCESSING, SPINNING, AND LIVING TEXTILES)
Campers will process the fibers cultured in the previous session and learn how to
spin, knit, and sew with filamentous algae without affecting their wellbeing.
OVERVIEW:
Spinning 101
Material considerations.
Designing with live phycotextiles.
Care and maintenance.
—
SESSION 3
(MACROALGAE)
Campers will become familiar with macroalgal materials, incl. alginates & sargasso
leather, applying novel extraction and production techniques.
OVERVIEW:
Macroalgal treatment.
Alginate extraction.
Phycomaterial extrusion.
17
BY: J. CELIS-RODRÍGUEZ
SESSION 1
MATERIALS PER CAMPER
● Cladophora spp. inoculum
● 1 round Arduino-compatible blue-spectrum LED lamp.
● Glue gun.
● 2L Mason Jar..
● 0.5m flexible nonreactive silicon hose, D=4mm
● Drill and 3mm glass-safe bit.
● 1 Arduino-compatible diaphragm pump.
● Arduino UNO.
● ~10 breadboard pin cables
● Arduino IDE (installed in laptop).
● 25g powdered Walne medium
● Mending needle (fine gage, steel)
● Carpentry clamp
—
SESSION 2
MATERIALS PER CAMPER
● Small screwable metal hook.
● Wooden rod, 50cm, D=6mm
● Wooden wheel.
● Handsaw or triangular file.
● Culture from last session.
● Preferred knitting equipment.
—
SESSION 3
MATERIALS PER CAMPER
● 100g sodium alginate.
● 20g calcium chloride
● 500mL water.
● 1 3mL intramuscular injection syringe.
● 500g Sargassus natansand/or kelp.
● 100mL vegetable oil
● 20mL glycerol.
● Hotplate
● Preferred knitting equipment.
● Sewing machine
● 3 spools thick cotton thread.
18
BY: J. CELIS-RODRÍGUEZ
THANK YOU.
19
BY: J. CELIS-RODRÍGUEZ

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Biotextile Maker Camp

  • 1. ◒ MAKERCAMP PROPOSAL B I O T E X T I L E S TAUGHT AND CONCEIVED BY J. CELIS-RODRÍGUEZ
  • 2. WEEK I BACTERIAL MATERIALS 1 BY: J. CELIS-RODRÍGUEZ
  • 3. 3 SESSIONS 2.5 HRS/SESSION SESSION 1 (CULTURING LEATHER ALTERNATIVES) Campers will learn about leather’s outsized environmental impact, the currently proposed alternatives, and a home-lab-friendly open-source method for the manufacture of bacterial leather, culturing custom SCOBYs to fit their textile needs. OVERVIEW: Fast Fashion, sustainability, and the leather industry. Textile alternatives and intro to further reading.. Bacterial culture practicum. Next-session preview — SESSION 2 (CULTURE AND TREATMENT OF BACTERIAL MATERIAL) Campers will learn how to propagate and continue their culture for long-term production, how to modify the composition and material results of said culture, and how to harvest, dry, and treat the resulting material for use in textile projects. OVERVIEW: Care, modification, and reproduction of textile culture. Harvest and treatment of bacterial leather (tanning, starch strengthening, etc.). Drying and surface patterns. — SESSION 3 (DESIGN AND MANUFACTURE OF BACTERIAL LEATHER PRODUCTS) Campers will learn and apply design and material property considerations for working with cultured material, and will deliver a final product of their own design (with assistance). OVERVIEW: Basic leatherwork design and material considerations. Cut, pattern, and sewing. Alternative methods and experimentation (wet fusion, weaving, blend-&-foam, etc.) 2 BY: J. CELIS-RODRÍGUEZ
  • 4. SESSION 1 MATERIALS PER CAMPER ● 1 spray bottle ● 1 plastic container >90cm2 ● 1m2 of porous fabric. ● 1 xacto knife or similar implement. ● Hotplate. ● Pot or recipient to boil water in (preferably ceramic or glass) ● Wooden spoon. ● Latex gloves (+ latex-free alternatives) ● 100 mL 70% ethanol. ● 1L water. ● 50g red hibiscus flower, o 20g Camelia sinensis ● ⅓ bottle of commercial, unpasteurized kombucha. ● 200g white sugar. ● Water-based food coloring (optional). — SESSION 2 MATERIALS PER CAMPER ● Culture from previous session. ● Latex gloves (+ latex-free alternatives) ● Spray bottle of 70% ethanol. ● 120cm thin, untreated wood or MDF (~3mm). ● 4 clamps or 4 wood screws ● Drill or electric screwdriver (of screws were chosen for the previous point). ● 50mL vegetable oil. ● 1 smooth cloth. ● 1 xacto knife. — SESSION 3 MATERIALS PER CAMPER ● Sewing machine. ● Set of fabric-cutting rulers. ● 3 spools of cotton thread. ● Sewing needle (medium gage, steel). ● Xacto, fabric scissors, architect’s knife. ● Plotter paper and fabric pencil. 3 BY: J. CELIS-RODRÍGUEZ
  • 6. 3 SESSIONS 2.5 HRS / SESSION SESSION 1 (BASIC PHYTOMATERIALS) Campers will become familiar with their human (and specifically Mexican) heritage through the discovery and manufacture of vegetable fiber textiles through modern techniques and technology applied to traditional methods. OVERVIEW: Human phytorelations and non-material value. Methods for the transformation of found phytomaterials. Modern applications for traditional methods. Design and material considerations. — SESSION 2 (SECONDARY PHYTOMATERIALS [4H]) Focus on phytomaterials derived from non-fibrous matter, plant waste, and chemical or biological reactions for the generation of zero-waste textiles. OVERVIEW: Textiles from juice and extracts. Textiles from fruit waste. Vegetable fiber reconstitution and biomaterial reinforcement. — SESSION 3 (PHYTOMATERIAL DESIGN & EXPERIMENTATION) Campers will learn to conceptualize and realize projects employing a variety of phytomaterials (incl. those developed in previous sessions, wood, MDF, cotton, etc.) with a consideration for material properties and design language, playing with texture, properties, and experimental production. OVERVIEW: Transversal phytomaterial considerations. Design, cutting, and construction. 5 BY: J. CELIS-RODRÍGUEZ
  • 7. SESSION 1 MATERIALS PER CAMPER ● Fallen branches, >1cm thick. ● Fallen leaves. ● Well-sharpened knife or blade. ● Manual whetstone. ● Sodium bicarbonate. ● 1L water ● Cooking pot. ● Hotplate. ● 50mL vegetable oil. ● 20mL glycerol. — SESSION 2 MATERIALS PER CAMPER ● 100g cornstarch. ● 50mL corn oil. ● 8 oranges ● 1 mesh sieve ● Pot ● Hotplate. ● Xacto knife. ● Whisk. ● Silicone spatula. ● Wax paper. ● 50mL vegetable oil ● 30mL glycerol. ● 1 fine metal grater ● 2g ground cinnamon ● 1 blender ● 10mL white vinegar ● 500mL water ● 1m2 cheesecloth ● 1 rolling pin ● 50g spent coffee grounds ● 10g sodium alginate ● 20g calcium chloride 6 BY: J. CELIS-RODRÍGUEZ
  • 8. SESSION 3 MATERIALS PER CAMPER ● Materials from the previous sessions. ● Sewing machine. ● Plotting paper. ● Xacto and fabric scissors. 7 BY: J. CELIS-RODRÍGUEZ
  • 10. 4 SESSIONS 4 HRS / SESSION SESSION 1 (MICRO-MYCO-LABORATORIES) Campers will be introduced to the fungal kingdom, its ecological role, and its potential for material innovation. They’ll learn and apply basic mycelial culture techniques, build portable microlaboratories, and upkeep and reproduce their first mycelial culture (Pleurotus spp.) OVERVIEW: Fungi. Fungal materials and the future of textile innovation. An introduction to mycelial culture. Design and construction of portable laboratories. Inoculum and mother cultures. — SESSION 2 (COMPOSITE, SELF-REPAIRING TISSUE) Focus on the culture and care of composite mycelial textiles and living textile management. OVERVIEW: Enclosure design and construction (bioreactors 101). Culture inoculation, scaling, and treatment. Composite material design and considerations. Composite use and the maintenance of living material. — SESSION 3 (PURE MYCOTEXTILES + DESIGN PT. 1) Campers will be introduced to pure mycotextiles and apply previous knowledge to the construction of an air-feed bioreactor for the culture of pure mycelium for foam, leather, and treated textile manufacture. OVERVIEW: Enclosure design and construction, pt. 2. Maintenance of pure mycocultures. Pure mycelium harvesting and treatment. Material considerations, patterns, and sewing. 9 BY: J. CELIS-RODRÍGUEZ
  • 11. SESSION 4 (DESIGN PT. 2) Campers will familiarize themselves with material considerations relevant to their cultured tissue, and will design and manufacture a final product of their own design. They will also have an opportunity to learn of advanced mycotextile techniques, including mycocircuitry and capacitive surfaces. OVERVIEW: Cut, patterns, and sewing, pt. 2. Use of non-textile mycomaterials. Finishing. 10 BY: J. CELIS-RODRÍGUEZ
  • 12. SESSION 1 MATERIALS PER CAMPER ● 1 alcohol lamp. ● Large steel forceps. ● 1 collapsible plastic container, 20L ● 1 aluminum can ● Xacto knife. ● Latex gloves. ● 2 petri dishes. ● 1 Mason Jar (200mL minimum). ● 9cm2 1cm-thickness polyethylene foam (or non-toxic equivalent). ● 1 spray bottle ● Scalpel, surgical. ● Hotplate. ● 250mL Erlenmeyer flask. ● Non-polymer towel. ● 1 glue gun, silicone. ● 1 drill. ● 1 black sharpie. ● 2 medium russet potatoes. ● 1L water. ● 20g agar agar ● 100mL ethanol 70% ● 1 medium fresh Oyster mushroom( Pleurotus spp.). — SESSION 2 MATERIALS PER CAMPER ● Pot ● Hotplate ● Mason Jar (200mL minimum) ● 3mL intramuscular injection syringe. ● Latex gloves. ● Hermetic seal plastic container, rectangular (30×20cm minimum). ● Xacto knife. ● 1 alcohol lamp. ● Culture from last session. ● Portable lab from last session. ● 1L water. ● 1 texture (hay, cork, paper, fabric scraps, etc.) ● 4 acid-free coffee filters. ● 1 black sharpie. 11 BY: J. CELIS-RODRÍGUEZ
  • 13. SESSION 3 MATERIALS PER CAMPER ● Hermetic seal plastic container, rectangular (30×20cm minimum). ● 3 30cm UV LED strips. ● 4 small-pore coffee filters ● 2 glass bottles or jars, hermetically sealable ● 2 large pieces of unpainted corrugated cardboard. ● 10cm flexible hose D=12mm ● 1m flexible hose Dint . =4mm ● 1 breadboard. ● ~30 breadboard pin cables ● 1 drill with 2mm metal-safe bit. ● Glue gun. ● Hotplate ● Pot. ● 3 spray bottle head ● 1 servo. ● 1 Arduino-compatible PC fan ● Arduino IDE (installed on laptop). ● 1 self-sealable injection port, D=1cm. ● 2 medium russet potato ● 1L water. ● 100mL 70% ethanol ● Electrical tape. ● Culture from session 4 ● Sewing machine ● 3 spools of cotton thread. ● Embroidery needle. (medium gage, steel) ● Textile result from past session. ● Scissors. ● Plotter paper. ● Pencil. — SESSION 4 MATERIALS PER CAMPER ● Sewing machine ● 3 spools of cotton thread ● Embroidery needle (medium gage, steel). ● Xacto knife ● Scissors. ● Plotter paper. 12 BY: J. CELIS-RODRÍGUEZ
  • 14. WEEK IV CULTURED DYES 13 BY: J. CELIS-RODRÍGUEZ
  • 15. 3 SESSIONS 4 HRS / SESSION SESSION 1 (VEGETABLE DYES) Campers will learn about sourcing and the dye and color process, and will explore our local ecosystem in order to ethically and sustainably select dye sources, and will apply pigment extraction, alteration, and fixing techniques. OVERVIEW: Dye and extraction. Ethical and local sourcing (w/ excursion). Pretreatment, mordants, y fixing. Liquid dye extraction. Powdered dye extraction and printing. Self-resupplying culture. — SESSIONS 2 Y 3 (LIVING MICROBIOLOGICAL DYES, PTs. 1 & 2) Campers will learn to isolate and culture pigment-producing microorganisms and live-dye textiles. OVERVIEW: Intro to microbiological dyes. Handprint/soil cultures & organism selection. ID techniques. Isolation and monobiotic culture. Live-dyeing. Care and knowledge of your bacterial partner. 14 BY: J. CELIS-RODRÍGUEZ
  • 16. SESSION 1 MATERIALS PER CAMPER ● Hotplate ● Pot. ● Wooden spoon. ● Knife. ● Cutting board. ● Scissors. ● 4L water. ● 30mL glycerol. ● At least 1m2 plant-based fabric (cotton, hemp, denim, bacterial leather, etc.) o 1 spool of yearn. ● 50mL white vinegar (3% AtAc) ● 10g alum. ● 100mL 70% ethanol. ● 1 plastic funnel. ● 2 coffee filters ● Alcohol lamp. ● Ash recipient. ● Request for leaves, branches, peels, etc. with interesting colors.. — SESSION 2 MATERIALS PER CAMPER ● Hotplate. ● Pot. ● Wooden spoon. ● Lab spatula. ● Alcohol lamp. ● 1 Mason Jar, hermetically sealable. ● 3 petri dishes,. ● Xacto knife. ● 1L water. ● 30mL glycerol. ● 100mL 70% ethanol. ● 20g agar agar ● 20g white sugar. ● 20g pea protein. 15 BY: J. CELIS-RODRÍGUEZ
  • 18. 4 SESSIONS 4 HRS / SESSION SESSION 1 (PHYCOFIBERS, WOOL, AND CULTURE) Campers will become familiar with microalgae, their material potential and growth habits, and will build a DIY minibioreactor for the culture of Cladophora spp. OVERVIEW: Algae and their habits. Liquid bioreactors: construction & design. Custom culture media. Microalgae inoculum and continuous culture. — SESSION 2 (PROCESSING, SPINNING, AND LIVING TEXTILES) Campers will process the fibers cultured in the previous session and learn how to spin, knit, and sew with filamentous algae without affecting their wellbeing. OVERVIEW: Spinning 101 Material considerations. Designing with live phycotextiles. Care and maintenance. — SESSION 3 (MACROALGAE) Campers will become familiar with macroalgal materials, incl. alginates & sargasso leather, applying novel extraction and production techniques. OVERVIEW: Macroalgal treatment. Alginate extraction. Phycomaterial extrusion. 17 BY: J. CELIS-RODRÍGUEZ
  • 19. SESSION 1 MATERIALS PER CAMPER ● Cladophora spp. inoculum ● 1 round Arduino-compatible blue-spectrum LED lamp. ● Glue gun. ● 2L Mason Jar.. ● 0.5m flexible nonreactive silicon hose, D=4mm ● Drill and 3mm glass-safe bit. ● 1 Arduino-compatible diaphragm pump. ● Arduino UNO. ● ~10 breadboard pin cables ● Arduino IDE (installed in laptop). ● 25g powdered Walne medium ● Mending needle (fine gage, steel) ● Carpentry clamp — SESSION 2 MATERIALS PER CAMPER ● Small screwable metal hook. ● Wooden rod, 50cm, D=6mm ● Wooden wheel. ● Handsaw or triangular file. ● Culture from last session. ● Preferred knitting equipment. — SESSION 3 MATERIALS PER CAMPER ● 100g sodium alginate. ● 20g calcium chloride ● 500mL water. ● 1 3mL intramuscular injection syringe. ● 500g Sargassus natansand/or kelp. ● 100mL vegetable oil ● 20mL glycerol. ● Hotplate ● Preferred knitting equipment. ● Sewing machine ● 3 spools thick cotton thread. 18 BY: J. CELIS-RODRÍGUEZ
  • 20. THANK YOU. 19 BY: J. CELIS-RODRÍGUEZ