This document provides an overview of Scintica's preclinical imaging product portfolio, including their technical capabilities. It describes several of Scintica's imaging systems - the Prospect T1 compact ultrasound system, M-Series compact MRI systems, FIVE2 fluorescence endomicroscopy, Newton 7.0 optical imaging system, and SuperArgus PET/CT systems. Sample images from live demonstrations of the Prospect T1, M-Series, and Newton 7.0 are also presented to showcase their imaging capabilities.

1. Katie Parkins, PhD
Preclinical Imaging Specialist
kparkins@scintica.com
Tonya Coulthard, MSc
Manager, Imaging Division
Tcoulthard@scintica.com
Overview of Scintica’s
Preclinical Imaging Product
Portfolio:
Technical Capabilities

2. Audience Poll

3. Katie Parkins, PhD
Preclinical Imaging Specialist
kparkins@scintica.com
Tonya Coulthard, MSc
Manager, Imaging Division
Tcoulthard@scintica.com
Overview of Scintica’s
Preclinical Imaging Product
Portfolio:
Technical Capabilities

4. • Prospect T1 – high frequency ultrasound
• M-Series – compact MRI
• FIVE2 – fluorescence in vivo eendomicroscopy
• Newton 7.0 – optical imaging
• SuperArgus – PET/CT
• Understanding the complementary nature of imaging modalities
Agenda

5. Prospect T1
Compact, High-Frequency, Tablet-based System
Small Animal Ultrasound

6. • Prospect T1 System Technical Overview
• Sample images acquired during the live virtual demo (Oct 1)
Topics of Discussion

7. Prospect T1 System Technical Overview
• System components and standard configuration
• Add-on hardware and software components

8. Prospect T1
System Components
• The Prospect T1 is the first tablet based high-frequency
ultrasound system specifically designed for pre-clinical
imaging of small animals
• System components:
• Tablet
• Probe
• Scanning Platform

9. Prospect T1
System Components:
Probes
• Three single element probes are available:
• 20 MHz (user selectable between 15-30 MHz)
• Primarily used for rat imaging, as well as harmonic contrast imaging
• 40 MHz (user selectable between 30-50 MHz)
• Primarily used for mouse imaging, and superficial anatomical
targets in larger species like rats
• 50 MHz (user selectable between 30-50 MHz)
• Primarily used for superficial anatomical targets in both mice and
rats

10. Prospect T1
System Components:
Scanning Platforms
• The platform is compact in design, again to limit the footprint of
the system
• The scanning platform has been designed for ergonomical
positioning of the probe
• Animal beds have integrated heating, and ECG and
respiratory monitoring
• Interchangeable beds are available for mice or rats
• Animal beds can be precisely adjusted in the X, Y, and Z axis

11. Standard System Configuration
• Standard system configuration for mouse
• B-Mode
• M-Mode
• Pulsed Wave / Color / Power / Tissue Doppler Mode
• Contrast Mode
• Comprehensive Measurement and Analysis Tools
• Scanning Platform – with mouse bed
• 40 MHz probe
• Standard system configuration for rat
• B-Mode
• M-Mode
• Pulsed Wave / Color / Power / Tissue Doppler Mode
• Contrast Mode
• Comprehensive Measurement and Analysis Tools
• Scanning Platform – with rat bed
• 20 MHz probe

12. Add-Ons:
3D Motor
• The 3D motor expands the capabilities of the Prospect T1 to
acquire 3D B-mode images
• Add-on includes the software analysis package to view the 3D
images and perform volume calculations

13. Add-Ons:
Image Guided Needle
Injection Mount
• The image guided needle injection mount integrates with probe
• Injections may be performed with a regular syringe and steel
needle, or pulled glass capillary needle
• Injections may be made into developing embryos, adult
myocardium, or abdominal/muscle targets
E15.5 mouse
embryo
Adult mouse
myocardium

14. Sample Images
• Images acquired during the live virtual demo on Oct 1
• Additional images to show capability not shown in the live demo

15. Tumour Imaging – 2D B-Mode Imaging
• Tumour identification, and basic linear and area measurements
• Investigation of surrounding structures
• Longitudinal imaging to monitor tumor progression or
therapeutic response
Orthotopic Mammary Fat Pad
Tumour MDA-MB-231
• Power Doppler (not shown during the demo due to a technical issue with the demo system)
can be used to assess tumor vasculature using endogenous signal within the tissue
• Contrast Imaging (linear and subharmonic - not shown during the demo as no contrast
agent was available) can be used to assess tumor microvasculature using microbubbles

16. Tumour Imaging – 2D B-Mode Imaging
• The complex nature of tumors can be investigated using B-Mode imaging, as well as surrounding structures
IP Injection of Ovarian Tumour Cells
SKOV-3
Tumour
Stomach
IP Injection of Ovarian Tumour Cells
SKOV-3
Kidney Splenic Vein
TumourIntestine

17. Tumour Imaging – 3D B-Mode Imaging
• 3D volume measurements
• Longitudinal imaging to monitor
tumor progression or therapeutic
response
Volume = 263mm3
Orthotopic Mammary Fat Pad
Tumour MDA-MB-231

18. Tumour Imaging – 3D B-Mode Imaging
• 3D volume measurements
• Complex tumor structures
can be visualized
• Longitudinal imaging to
monitor tumor progression or
therapeutic response
IP Injection of Ovarian Tumour Cells
SKOV-3
Tumo
ur

19. Cardiovascular Imaging – Parasternal Long Axis View of LV
B-Mode Frame Rate = 30fps ECG Gated Kilohertz Visualization =
30fps

20. Cardiovascular Imaging – Parasternal Short Axis View of LV

21. Cardiovascular Imaging – Pulsed Wave Doppler
Pulmonary Artery Mitral Valve

22. Cardiovascular Imaging – Left Carotid Artery
• Color Doppler (not shown during the demo due to a technical issue with the demo system)
can be used to identify and assess blood flow velocity and direction within the heart and
surrounding vasculature

23. M-Series
Compact, Self-Shielded, High Performance Permanent
Magnet
Small Animal MRI

24. Topics of Discussion
• M-Series System Technical Overview
• Sample images acquired during the live virtual demo (Oct 1)

25. M-SeriesTM System Technical Overview
• M-SeriesTM Compact magnet design
• Animal handling system
• SimPET insert option for simultaneous PET/MR imaging

26. System Components
• Compact magnet – select either the M3, M5, or M7 magnet;
• Electronics cabinet and User Workstation - is the same for all
systems.
• Accessories and add-ons
• Animal handling system with heating and physiological monitoring
• Anesthesia delivery and exhaust gas scavenging
• SimPETTM insert – simultaneous PET/MRI (M7 system only)

27. M-SeriesTM Compact MRI Systems from Aspect Imaging
M3 – Mouse Only M7 – Mouse & Large RatsM5 – Mouse & Small Rats

28. M-SeriesTM Compact Magnet Design
• Require no special infrastructure
• Compact and self shielded with minimal external fringe field
• Operate very quietly during image acquisition
• Systems are installed with first images being acquired in less
than 1 day
 Installed within an animal facility, or existing laboratory
next to other equipment or furnishings

29. Animal Handling System
• Fully integrated animal handling and coil system includes
• Mouse or rat beds to suite varying animal sizes
• Anatomy specific coils, with automatic tuning
• Heating
• Physiological monitoring
• Anesthesia delivery and scavenging

30. Imaging Coils
Type
Dimensions
Application
Inner Diameter Length
Mouse Head 23 mm 25 mm Neurological imaging in mice
Mouse Body 30 mm 50 mm
Extremity, abdominal, and thoracic cavity imaging in
mice
Mouse Whole Body 30 mm 80 mm Whole body imaging in mice
Large Mouse Body 38 mm 50 mm
Extremity, abdominal and thoracic cavity imaging in
large/obese mice
Rat Head 35 mm 40 mm Neurological imaging in rats
Rat Body 50/60 mm ellipsoid 90 mm
Extremity, abdominal and thoracic cavity imaging in
rats
Large Rat Body 71 mm 90 mm
Extremity, abdominal and thoracic cavity imaging in
large rats
Imaging coils should fit as tightly as
possible to the anatomical target for high
quality images

31. Most measurements and
parameters are functions of
time, so we need waveforms

32. SimPET insert option for simultaneous PET/MR imaging
• The SimPETTM insert expands the capabilities of the M7 system to allow
for simultaneous PET/MR imaging
• MR images compliment the highly sensitive PET images in detecting
functional information, abnormalities, and early disease, providing an
anatomical context

33. Sample Images
• Images acquired during the live virtual demo on Oct 1

34. Tumour Imaging – Orthotopic Mammary Fat Pad Tumour
• MDA-MB-231 cell line
• T1 and T2 weighted image contrast helps
to identify anatomy; along with
pathological changes, including tumors
• 250µm in-plane resolution with 1mm slice
thickness
• Acquisition was around 4.5 minutes
T1 Weighted T2 Weighted

35. Tumour Imaging – Orthotopic Mammary Fat Pad Tumour
• Tumour volume was found to be 273mm3
T1 Weighted T2 Weighted
MDA-MB-231

36. Tumour Imaging – IP Injected Ovarian Tumour Cells
• SKOV3 cell line, injected IP
• These tumors may form anywhere within the
peritoneal cavity
• Numerous tumors were located throughout the
abdomen
T2 WeightedT1 WeightedT1 Weighted T2 Weighted

37. Tumour Imaging – IP Injected Ovarian Tumour Cells
• VivoQuant was used to visualize the acquired images in 3D and
to quantify the tumor volumes.
• Various manual and semi-automatic tools are available
T2 WeightedT1 Weighted
Region Of
Interest
Color
Volume
(mm³)
Upper Tumour red 144
Mid Tumour green 6
Lower Tumour blue 9
Lower Tumour #2 cyan 7
Mid Tumour #2 magenta 64

38. Brain Imaging – Normal Brain
T2 WeightedT1 Weighted
• Various structures can be visualized within the brain
• 200µm in-plane resolution, 1mm slice thickness; 7.5-minute acquisition time

39. FIVE2 ViewnVivo
Fluorescence in vivo endomicroscopy

40. Newton 7.0 FT
In Vivo Optical Imaging System
COURTESY : Matthieu Germain , Nanobiotix/Curadigm

41. TOPICS OF DISCUSSION
• Applications of optical imaging
• Product overview
• Images acquired during the live demo

42. Main applications of optical imaging
Luciferase
expressing
metastases
mCherry
expressing
cancer cells
Fluorescence Imaging Bioluminescence Imaging
• Oncology
• Neurology
• Biodistribution studies
• Treatment studies (BLI)
• Cell tracking
• Ex vivo imaging

43. Proprietary CCD Camera
16-bit Scientific CCD
Camera
Grade 0 – No dead pixels
NIST calibrated
4.8 Orders of Magnitude
Key Technical Features of the Newton 7.0 FT
CAMERA
DARQ-9

44. Key Technical Features of the Newton 7.0 FT
CAMERA
RESOLUTION
4.6 MP
Newton 7.0 FT / 4.6MP Other systems / >1MP
Better Performance in patter recognition

45. Key Technical Features of the Newton 7.0 FT
LENS APERTURE
F/0.70
Newton 7.0 FT = f/0.70 Other systems = f/0.95
More light collection = More sensitivity
Wider lens
aperture
Smaller lens
aperture

46. Key Technical Features of the Newton 7.0 FT
MOTORIZED
DARKROOM
Infinite imaging Capabilities

47. Key Technical Features of the Newton 7.0 FT
Green
520nm
Green
580nm
Red
640nm
IR
740nm
Blue
480nm
NIR
680nm
IR
780nm
Blue
420nm
Full Spectrum Tunability
• 8 Excitation channels (400nm > 800nm)
• Powerful Laser Class II illumination
• Reducing the cross stimulation
• Increasing the sensitivity of your
images

48. Key Technical Features of the Newton 7.0 FT
NARROW BAND-PASS FILTERS
Emission Filters
• 8 Narrow Bandpass emission filters (500nm >
900nm)
• Dual Magnetron sputter-coated technology
• Ensuring transmission above 90%
• Improving spectral separation for multi-spectral
imaging

49. Key Technical Features of the Newton 7.0 FT
CCD camera
3D
NIR
Camer
a
3D
NIR
Camer
a
How 3D Optical Tomography is achieved in the Newton
7.0 ?
• First, the Topographic image of the mouse is acquired with
the help of 3D NIR Cameras
• Then, the main CCD camera acquires multiple images of
the signal using various emission filters from wavelengths
in the spectral emission of the specific probe used.
• The signal is reconstructed with the use of an algorithm
and repositioned within the topographic image of the
animal.

50. Key Technical Features of the Newton 7.0 FT
Co-registration with digital Organ & Bones
Atlas
• Choose to add or hide individual organs and
bones
• Axial, Sagittal and Coronal Views
• Quantification of the signal in units of volume

51. Images acquired during the live demo
Signal Only Image Brightfield Image Overlay Image

52. Images acquired during the live demo
IP metastasis model
(human ovarian cancer)
Dual MFP Model
(human breast cancer)

53. FLI Phantom @
480nm
Multiplex Phantom
Images acquired during the live demo
Fluorescent
beads
Fluorescent
beads
Luminescent
beads

54. SuperArgus
State-of-the-Art Pre-Clinical PET/CT Systems
Courtesy of Dr. M. Desco & J.J. Vaquero, UMCE Hospital Gregorio Marañón HGUGM (Madrid, Spain)

55. TOPICS OF DISCUSSION
• Product Overview
• What Makes the Sedecal Systems Unique
• Multi-animal Handling System

56. • Self-shielded to meet FDA guidelines
• no special room preparation, controls, or
additional shielding required
• Systems can easily be placed within existing
laboratory environments, imaging cores, or
within the animal facility
• Can have the following integrated into animal
handling:
• Anesthesia – isoflurane with gas scavenging
• Heating
• Physiological monitoring
• Image may be saved as DICOM, Interfile, or JPEG
REVIEW OF SUPERARGUS AND COMPACT MODELS

57. REVIEW OF SUPERARGUS AND COMPACT MODELS
• Compact (Mouse only) Model
• PET or CT
• Dimensions : 60 x 60 x 40cm
• 55 mm bore
• Static FOV: 45mm axial; 100mm
trans-axial
• CT Specifications
• 65µm resolution (max.)
• 15sec acquisition time (min.)
• PET Specifications
• 32 tDOI Phoswitch detectors; 4 rings
• ≤1.0mm resolution using 3D OSEM
reconstruction (≤2 min to perform)

58. • Super Argus Models (PET and/or CT)
• r models – mouse, rat, marmoset
• 90mm bore
• FOV – 220mm axial (dynamic); 80mm transaxial
• R models – up to 3kg rabbit
• 160mm bore
• FOV – 350mm axial (dynamic); 120mm transaxial
• P models – up to non-human primate, canine, porcine, etc.
• 260mm bore
• FOV – 650mm axial (dynamic); 210mm transaxial
• 2, 4, or 6 PET ring options for a 50mm, 100mm, or 150mm fixed axial FOV
• CT is focused on low does, high resolution (15µm) rapid scanning options (15sec)
REVIEW OF SUPERARGUS AND COMPACT MODELS

59. • Core Phoswich detector technology along with the electronics provide the following best in
class specifications:
• Highest resolution on the market (≤1.0mm)
• Resolution uniformity across the entire FOV, with FOV filling majority of the bore size due
to the tDOI technology correcting the parallax error
• Highest sensitivity (11%; at 100-700keV)
• Real-time imaging (up to 2.5msec frame rate if desired)
• Dynamic imaging – possible using time stamp acquisition electronics
• Parallel electronics acquisition
SUPERARGUS IS CONSIDERED TO BE BEST IN CLASS

60. MULTI-ANIMAL HANDLING SYSTEM
• Up to 4 mice can be scanned simultaneously in the R model
(160mm bore) using the multi-animal handling system
• Individually controlled anesthesia flow and heating controls
• Separate animal preparation workstation is also included
• Additional configurations are available for rats and/or mice
depending on specific needs

61. MULTI-ANIMAL HANDLING SYSTEM
Resolution and
sensitivity are
maintained throughout
the entire FOV due to
the unique tDOI
Phoswich detectors –
correcting for the
parallax error

62. • Sample images acquired during the live virtual demo (Oct 1)
Understanding the Complementar y Nature of Imaging Modalities

63. Understanding the Complementar y Nature of Imaging Modalities
T1 Weighted
• Bioluminescence helps to identify where the tumors may be located, however differentiating tumors from one another, and
measuring tumor volume is better done using an anatomical imaging modality such as MRI or Ultrasound
Kidney Splenic Vein
TumourIntestine
IP Injection of SKOV3 Ovarian
Tumour Cells

64. Understanding the Complementar y Nature of Imaging Modalities
T2 Weighted
• Bioluminescence helps to confirm viability of the tumor cells, as they express luciferase, approximate volumes may be
possible from the BLI signal; anatomical images help to confirm tumor volume - ultrasound (263mm3) or MRI (273mm3)
Orthotopic Mammary Fat Pad
Tumour (MDA-MB-231)

65. Audience Poll

66. Katie Parkins, PhD
Preclinical Imaging Specialist
kparkins@scintica.com
Tonya Coulthard, MSc
Manager, Imaging Division
Tcoulthard@scintica.com
Overview of Scintica’s
Preclinical Imaging Product
Portfolio:
Technical Capabilities
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