Photosynthesis:
The Calvin Cycle
Life from Air

AP Biology

2007-2008
Whoops! Wrong Calvin…
The Calvin Cycle

AP Biology

1950s | 1961
Remember what it means to be a plant…

 Need to produce all organic molecules
necessary for growth


carbohydrates, lipi...
Light reactions

 Convert solar energy to chemical
energy

ATP



ATP → energy



NADPH → reducing power

 What can we...
How is that helpful?
 Want to make C6H12O6
synthesis
 How? From what?
What raw materials are available?


CO2
NADPH

ca...
From CO2 → C6H12O6
 CO2 has very little chemical energy


fully oxidized

 C6H12O6 contains a lot of chemical energy

...
From Light reactions to Calvin cycle
 Calvin cycle


chloroplast stroma

 Need products of light reactions to
drive syn...
C

Calvin cycle
1C

C C C C C
C C C C C

3. Regeneration
C C C C C
of RuBP

RuBP

starch,
sucrose,
cellulose
& more

ribul...
Remember
G3P?
glycolysis

glucose

C-C-C-C-C-C

2 ATP
2 ADP

fructose-1,6bP
P-C-C-C-C-C-C-P

DHAP

P-C-C-C

G3P

glycerald...
To G3P and Beyond!
 Glyceraldehyde-3-P

To G3P
and beyond!

end product of Calvin cycle
 energy rich 3 carbon sugar
 “C...
RuBisCo
 Enzyme which fixes carbon from air
ribulose bisphosphate carboxylase
 the most important enzyme in the world!
...
Accounting
 The accounting is complicated


3 turns of Calvin cycle = 1 G3P



3 CO2 → 1 G3P (3C)



6 turns of Calvin...
Photosynthesis summary
 Light reactions
produced ATP
 produced NADPH
 consumed H O
2
 produced O as byproduct
2


 C...
Light Reactions
light → ATP + NADPH + O
2
energy

H 2O +
H2O
sunlight

Energy Building
Reactions

NADPH

ATP
AP Biology

O...
Calvin Cycle
CO2 + ATP + NADPH → C6H12O6 + ADP + NADP
CO2
ADP

NADP

Sugar
Building
Reactions

NADPH

ATP
AP Biology

suga...
Putting it all together
light
CO2 + H2O + energy → C6H12O6 + O2
H2O

CO2

sunlight
ADP
Energy NADP
Building
Reactions

Sug...
even though
this equation
is a bit of a lie…
it makes a
better story

Energy cycle
sun
Photosynthesis
light
CO2 + H2O + en...
Summary of photosynthesis
6CO2 + 6H2O + light → C6H12O6 + 6O2
energy

 Where did the CO2 come from?
 Where did the CO2 g...
Supporting a biosphere
 On global scale,
photosynthesis is the
most important process
for the continuation of life on Ear...
The poetic perspective…
 All the solid material of every plant


was built by sunlight out of thin air
All the solid mat...
If plants can do it…
You can learn it!
Ask Questions!!

AP Biology

2007-2008
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  • CO2 contains little energy because it is fully oxidized
    Reduce CO2 in a series of steps to synthesize a stable energy storage molecule
  • 1. A five-carbon sugar molecule called ribulose bisphosphate, or RuBP, is the acceptor that binds CO2 dissolved in the stroma. This process, called CO2 fixation, is catalyzed by the enzyme RuBP carboxylase, forming an unstable six-carbon molecule. This molecule quickly breaks down to give two molecules of the three-carbon 3-phosphoglycerate (3PG), also called phosphoglyceric acid (PGA).
    2. The two 3PG molecules are converted into glyceraldehyde 3-phosphate (G3P, a.k.a. phosphoglyceraldehyde, PGAL) molecules, a three-carbon sugar phosphate, by adding a high-energy phosphate group from ATP, then breaking the phosphate bond and adding hydrogen from NADH + H+.
    3. Three turns of the cycle, using three molecules of CO2, produces six molecules of G3P. However, only one of the six molecules exits the cycle as an output, while the remaining five enter a complex process that regenerates more RuBP to continue the cycle. Two molecules of G3P, produced by a total of six turns of the cycle, combine to form one molecule of glucose.
  • Gluconeogenesis == making new glucose
  • Where did the CO2 come from?air
    Where did the CO2 go?carbohydrate (G3P)
    Where did the H2O come from?soil (xylem)
    Where did the H2O go?split to donate e- & H+
    Where did the energy come from?sun
    What’s the energy used for?to make ATP in light reactions
    What will the C6H12O6 be used for?the work of plant life
    Where did the O2 come from?splitting of H2O
    Where will the O2 go?air or respiration
    What else is involved…not listed in this equation?
    ATP, ADP, NADP, NADPH
  • 47 ch10calvincycle2008

    1. 1. Photosynthesis: The Calvin Cycle Life from Air AP Biology 2007-2008
    2. 2. Whoops! Wrong Calvin… The Calvin Cycle AP Biology 1950s | 1961
    3. 3. Remember what it means to be a plant…  Need to produce all organic molecules necessary for growth  carbohydrates, lipids, proteins, nucleic acids  Need to store chemical energy (ATP) produced from light reactions in a more stable form  that can be moved around plant  saved for a rainy day  carbon + water + energy → glucose + oxygen dioxide light → C H O + 6O AP Biology6CO2 + 6H2O + 6 12 6 2 energy
    4. 4. Light reactions  Convert solar energy to chemical energy ATP  ATP → energy  NADPH → reducing power  What can we do now? → → build stuff !! AP Biology photosynthesis
    5. 5. How is that helpful?  Want to make C6H12O6 synthesis  How? From what? What raw materials are available?  CO2 NADPH carbon fixation reduces CO2 NADP C6H12O6 AP Biology NADP
    6. 6. From CO2 → C6H12O6  CO2 has very little chemical energy  fully oxidized  C6H12O6 contains a lot of chemical energy  highly reduced  Synthesis = endergonic process  put in a lot of energy  Reduction of CO2 → C6H12O6 proceeds in many small uphill steps each catalyzed by a specific enzyme  using energy stored in ATP & NADPH  AP Biology
    7. 7. From Light reactions to Calvin cycle  Calvin cycle  chloroplast stroma  Need products of light reactions to drive synthesis reactions ATP  NADPH  ATP thylakoid AP Biology stroma
    8. 8. C Calvin cycle 1C C C C C C C C C C C 3. Regeneration C C C C C of RuBP RuBP starch, sucrose, cellulose & more ribulose bisphosphate 3 ATP H H H | | | C–C–C AP Biology C C C C C C C C C C C C C C C C C C CO2 1. Carbon fixation C C C C C C RuBisCo ribulose bisphosphate carboxylase 3 ADP used to make glucose C=C=C 5C C C 6C C C C C C C C C C C C C 5C glyceraldehyde-3-P G3P 3C C C C phosphoglycerate 6 NADP 3C C C C C C C 6 ATP 2. Reduction 6 NADPH PGA C C C C C C 3C 6 ADP C C C C C C H | H | H |
    9. 9. Remember G3P? glycolysis glucose C-C-C-C-C-C 2 ATP 2 ADP fructose-1,6bP P-C-C-C-C-C-C-P DHAP P-C-C-C G3P glyceraldehyde 3-phosphate C-C-C-P 2 NAD+ 2 4 ADP AP Biology Photosynthesis pyruvate C-C-C 4 ATP
    10. 10. To G3P and Beyond!  Glyceraldehyde-3-P To G3P and beyond! end product of Calvin cycle  energy rich 3 carbon sugar  “C3 photosynthesis”   G3P is an important intermediate  G3P → → glucose → → carbohydrates → → lipids → → phospholipids, fats, waxes → → amino acids → → proteins → → nucleic acids → → DNA, RNA AP Biology
    11. 11. RuBisCo  Enzyme which fixes carbon from air ribulose bisphosphate carboxylase  the most important enzyme in the world!   it makes life out of air!  definitely the most abundant enzyme I’m green with envy! AP Biology It’s not easy being green!
    12. 12. Accounting  The accounting is complicated  3 turns of Calvin cycle = 1 G3P  3 CO2 → 1 G3P (3C)  6 turns of Calvin cycle = 1 C6H12O6 (6C)  6 CO2 → 1 C6H12O6 (6C)  18 ATP + 12 NADPH → 1 C6H12O6  AP Biology any ATP left over from light reactions will be used elsewhere by the cell
    13. 13. Photosynthesis summary  Light reactions produced ATP  produced NADPH  consumed H O 2  produced O as byproduct 2   Calvin cycle consumed CO2  produced G3P (sugar)  regenerated ADP  regenerated NADP  AP Biology ADP NADP
    14. 14. Light Reactions light → ATP + NADPH + O 2 energy H 2O + H2O sunlight Energy Building Reactions NADPH ATP AP Biology O2  produces ATP  produces NADPH  releases O2 as a waste product
    15. 15. Calvin Cycle CO2 + ATP + NADPH → C6H12O6 + ADP + NADP CO2 ADP NADP Sugar Building Reactions NADPH ATP AP Biology sugars  builds sugars  uses ATP & NADPH  recycles ADP & NADP  back to make more ATP & NADPH
    16. 16. Putting it all together light CO2 + H2O + energy → C6H12O6 + O2 H2O CO2 sunlight ADP Energy NADP Building Reactions Sugar Building Reactions NADPH ATP AP Biology O2 sugars Plants make both:  energy  ATP & NADPH  sugars
    17. 17. even though this equation is a bit of a lie… it makes a better story Energy cycle sun Photosynthesis light CO2 + H2O + energy → C6H12O6 + O2 plants CO2 H 2O animals, plants glucose ATP C6H12O6 + O2 → energy + CO2 + H2O Cellular Respiration AP Biology The Great Circle of Life,Mufasa! ATP O2
    18. 18. Summary of photosynthesis 6CO2 + 6H2O + light → C6H12O6 + 6O2 energy  Where did the CO2 come from?  Where did the CO2 go?  Where did the H2O come from?  Where did the H2O go?  Where did the energy come from?  What’s the energy used for?  What will the C6H12O6 be used for?  Where did the O2 come from?  Where will the O2 go?  What else is involved…not listed in this equation? AP Biology
    19. 19. Supporting a biosphere  On global scale, photosynthesis is the most important process for the continuation of life on Earth  each year photosynthesis…  captures 121 billion tons of CO2  synthesizes 160 billion tons of carbohydrate  AP Biology heterotrophs are dependent on plants as food source for fuel & raw materials
    20. 20. The poetic perspective…  All the solid material of every plant  was built by sunlight out of thin air All the solid material of every animal was built from plant material air AP Biology sun Then all the plants, cats, dogs, elephants & people … are really particles of air woven together by strands of sunlight!
    21. 21. If plants can do it… You can learn it! Ask Questions!! AP Biology 2007-2008

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