Final B.Sc. important questions biochemistry


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According to Andhra University syllabus. The file is prepared based on the old papers for the students

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Final B.Sc. important questions biochemistry

  1. 1. Page 1BiochemistryQuestions from Previous Papers and Expected Questions for B.Sc. StudentsPrepared byI.SATISH KUMARhttp://biochemden.inFor more Study materials andOld papers, do not forget to visit our site:http://biochemden.in
  2. 2. Page 2B.Sc. Biochemistry Questions ListFinal Year (IIIrd Paper)Unit.I: Human PhysiologyEssays: (Each carries 15Marks)1. Give the composition of Blood & Write the process of Blood Clotting?2. What are Thyroidal Hormones? Write about the secretion & Action of ThyroidHormones?3. Describe the mechanism of action of Steroidal Hormones. Write about GonadalHormones.4. Describe the structures & action of Thyroid & Parathyroid Hormones.5. Write an account of Gastrointestinal Hormones & their functions?6. Explain the chemistry & Physiological role of Posterior pituitary Hormones?7. Describe the chemistry, Physiological role & disorders of Adrenal MedullaryHormones.8. Describe the mechanism of action of Peptide hormones9. Describe the different adrenal hormones, their chemistry & function.10. What is Digestion? How the carbohydrates are digested in GIT and itsabsorption?11. Write the structure of Myocyte (Muscle Cell)? Describe its Muscular Contractionmechanism.Short Questions (Each carries 4 Marks):1. Composition of Blood2. Neurotransmitters3. Glucagon4. Glucocorticoids5. Estrogens6. Absorption of Carbohydrates7. CO2 Transport in Blood8. Gastro Intestinal Hormones (GIT Hormones)9. Posterior Pitutary Hormones10. Composision of Gastric Juice11. Skeletal Muscle Structure12. Steroidal hormone mechanismVery Short Questions (Each carries 2 Marks)1. Gastric Juice2. Actin & Myosin3. Blood Groups4. Calcitriol5. Sarcomere6. CSF7. Anemia8. Cushing’s Syndrome9. PTH10. ESR11. Estrogens12. ADH (Antidiuretic Hormones)13. Glucagon14. Neurotransmitters15. Bilirubin16. Posterior pituitary Hormones17. cAMP (Cyclic AMP structure)18. Pepsin19. ACTH20. Glutathione21. Adrenalin & Nor-adrenalin22. HRE23. G-Protein24. CCK & Pepsin25. Granulocytes
  3. 3. Page 3Unit.II: Food & NutritionEssays: (Each carries 15Marks)1. Describe the determination of calorific values of Foods by Bombcalorimeter. Give the calorific values of Carbohydrates & fats.2. Write the dietary sources, Physiological role & deficiency diseases ofVitamin B6 & C.3. What is BMR? Write about the factors that affect BMR?4. What are the Macro elements in Human nutrition? Write theirNutritional importance?5. Write about the biological value of Protein. Give the sources ofComplete and Incomplete proteins.6. Describe the Structure & Biological function and disorders of Thiaminand Niacin7. Explain the Biological value of Protein calorie Malnutrition.Short Questions: (Each 4 Marks)1. Calorific values of Foods2. Fat-Soluble vitamins3. Balanced Diet4. Marasmus5. Dietary requirement of Pregnant women6. Dietary requirement of Pregnant women & Lactating women7. Nutritional importance of Selenium (Se) & Megnesium (Mg)8. Kwashiorkor9. Complete & Incomplete Proteins10. SDA (Specific Dynamic Action)11. Obesity & objectionsVery Short Questions (Each carries 2 Marks)1. SDA (Specific Dynamic Action)2. Biological Value of Proteins3. Calorific value of Foods4. Balanced diet5. Kwashiorkor6. Fat-Soluble vitamins7. Essential Amino acids8. Complete ProteinsFor more Study materials andOld papers, do not forget to visit our site:http://biochemden.in
  4. 4. Page 4Unit.III: Clinical BiochemistryEssays: (Each carries 15Marks)1. Write the structure of Nephron & Formation of Urine2. Describe the structure & Functions of Liver3. What are the Buffer systems in Blood & How do they maintain Acid-Base balance in Blood4. Write a note on Liver Function Tests (LFT)5. Describe the role of Kidney in maintaining acid-base & electrolytebalance.6. What is Anemia? How the anemia classified? Write about thetreatment?7. Describe about Diabetes? Write about the types, causes andcontrolling methods of Diabetes?Short Questions (Each carries 4 Marks)1. Nephron Structure2. Jaundice3. Sickle cell anemia4. Serum markers for Heart Diseases5. Plasma Proteins in health & disease6. Anemia7. Thalassemia8. Hemophilia9. Serum enzymes in Liver diseases (SGPT,SGOT and ALP)10. GTT (Glucose Tolerance Test)11. AtherosclerosisVery Short Questions (Each carries 2 Marks)1. Diabetes2. SGOT & SGPT3. Plasma Lipoproteins4. Renal Thresh hold5. Hippuric acid6. Hepatitis7. Cirrohosis8. Hyperglycemia9. Hypoglycemia10. Glycosuria11. Fatty liver12. Lipoproteinemia13. CR Proteins (C-reactive proteins)14. Detoxification of liverFor more Study materials andOld papers, do not forget to visit our site:http://biochemden.in
  5. 5. Page 5Unit.IV: ImmunologyEssays (Each carries 15 Marks)1. How Immunoglobulins are classified? Write the Structure of Ig.G?2. What is vaccine? How are the different types of vaccines produced?3. Differentiate between Humoral and Cell mediated Immunity?4. What is Immunity? Write about Innate Immunity?5. Describe any FOUR antigen-antibody interactions. & its biologicalfunctions.6. Describe the cell-mediate immunity7. Describe cell mediated & Humoral immunity?8. Write the structure of Ig.G & mention the different classes of Igs?9. Describe the synthesis of Monoclonal antibodies and its biologicalfunctions.10. What is hypersensitivity? Explain its types?11. Write about Cells involved in Immune defense mechanism in Humanbody.Short Questions (Each carries 4 Marks)1. Adjuvants2. Cell mediated immunity3. Ig.G Structure4. Modern Vaccines5. Humoral immunity6. Monoclonal Antibodies7. Autoimmunity8. Vaccines9. Clonal selection theory10. Adjuvants11. RIA12. B-cell & T-cell13. Phagosytosis and its mechanism14. Agglutination15. Precipitation16. Blood group antigens17. Immunodiffusion18. ELISA & its typesVery Short Questions (Each carries 2 Marks)1. Epitopes2. Vaccines3. Haptens4. Adjuvants5. Autoimmunity6. Agglutination7. ELISA8. Monoclonal Antibodies9. Paratopes (or) Antigenic determinants10. Agglutinins11. Precipitins12. Plasma cells & Memory cells13. Antigen & Antigenicity14. Immunogen & Immunogenesity15. Phagocytosis16. Toxoids17. Attenuated Vaccines18. Graft rejection
  6. 6. Page 6B.Sc. Biochemistry Questions ListFinal Year (IVth Paper)Unit. I: MicrobiologyEssays: (Each carries 15Marks)1. Describe the methods of isolation of pure cultures of bacteria & theircultivation?2. Describe the lytic & lysogenic life cycles of lambda phage.3. Describe the bacterial growth curve & its kinetics.4. Write an account of viruses.5. Describe Gram’s Staining. Write the differences between Gram positiveand Gram Negative bacteria.6. Write the structure of TMV & Lytic growth of Lambda phage.7. Write the classification & structure of VirusesShort Questions (Each carries 4 Marks):1. Bacterial growth curve2. Gram’s Staining3. Prokaryotic microorganisms4. Differences between Gram +ve and –ve bacteria5. Enriched media6. Isolation of Bacteria7. One step growth8. Conjugation9. Industrial uses of Aspergillus niger, Yeast & Spirullina10. Mycoplasma11. Plaque Forming Unites (PFU)12. What is Sporulation and write about bacterial endospores?13. Serial dilution method14. Pour plate method15. Streak plate method16. Bacterial Motility17. Structure of TMVVery Short Questions (Each carries 2 Marks)1. Prions2. Enriched media3. HIV4. Template5. Spores6. Synchronous culture7. Prokaryotes8. Heterotroph9. Chemotactic culture10. Virion11. Batch culture12. Mycoplasma13. What is a Cyst14. Gram negative cell15. Gram Positive cell16. Colony forming units
  7. 7. Page 7Unit.IIDNA Replication & TranscriptionEssays: (Each carries 15Marks)1. Write the experimental proof for the semi-conservative method ofReplication of DNA2. Describe the Topoisomerase.3. Describe the synthesis of RNA in bacteria (or) Describe the Transcriptionprocess in bacteria4. Describe the Replication of Prokaryotic DNA5. Compare the RNA polymerases of Prokaryotes with those of eukaryotes.6. Describe the different DNA polymerases and their role in DNA replication7. Write the mechanism of eukaryotic gene transcription?8. Write the difference between Prokaryotic and eukaryotic DNApolymerases?9. Organization of Eukaryotic genome.10. Describe the proof of Nucleic acids act as Genetic material11. Describe the step of Initiation, Elongation and Termination of DNAReplication.12. Transcriptional events in eukaryotic mRNA synthesisShort Questions (Each carries 4 Marks):1. Okazakii fragments2. DNA polymerase.III of E.coli3. Meselson - stahl’s experiment4. Termination of Gene transcription in prokaryotes5. Differences between Ribosomes of Prokaryotes & Eukaryotes6. Conjugation in Bacteria7. Leading & Lagging strand of DNA8. Prokaryotic RNA polymerase9. Transcription factors10. Leading & lagging strand syntheisis11. Rho dependent & Rho independent factorsVery Short Questions (Each carries 2 Marks)1. Promoter2. Primer3. Gene4. Sigma factor5. Inhibitors of DNA replication6. Okazakii fragments7. Primosome8. DNA gyrase9. Replicon10. Sense and Antisense strands11. Reading frame12. Actinomycin.D13. Rho-factor14. Primosome complex15. Leading & lagging strands16. RNA Primer
  8. 8. Page 8UNIT.III: Genetic code, Translation&Regulation of Gene expression in ProkaryotesEssays: (Each carries 15Marks)1. Describe the regulation of Gene expression in prokaryotes2. Describe the Genetic Code and its characteristics3. Describe the Genetic code. How was it deciphered?4. Describe the experiments of Nirenberg & Khorana in deciphering theGenetic code.5. Describe the Protein Synthesis in Prokaryotes?6. What is Genetic code? Describe the salient features of Genetic Code?7. Describe the regulation of Gene expression in prokaryotes8. Describe the structure of tRNA and its role in protein synthesis.9. Write the role & mechanism of Amino acyl tRNA synthatase in proteinsynthesis.Short Questions (Each carries 4 Marks):1. Wobble hypothesis2. Post translational modifications3. Lac operon4. tRNA5. Mechanism of Amino acyl tRNA synthetases6. Eukaryotic Ribosomes7. Catabolite RepressionVery Short Questions (Each carries 2 Marks)1. Signal peptide (or) Signal sequence2. Ribosomes3. Genetic code4. Post translational modifications5. Puromycin6. Operon7. Signal hypothesis8. WobbleVIZIANAGRAM BRANCHOPP0SITE DARGAH, KOTA Jn, VIZIANAGRAM. Ph:9440011678M.Sc. ENTRANCE EXAMS SHORT TERM COACHING-2012*BIOTECHNOLOGY *MICROBIOLOGY*BIOCHEMISTRY *FOOD TECHNOLOGYCoaching for M.Sc. Entrance- Local & Central universities(PCU, HCU….)Secured top 10 ranks in Central & Local Universities EntranceExaminations from the past 8 yearsFor further details Contact: 9440011678, 9440715785
  9. 9. Page 9UNIT.IV: Recombinant DNA Technology &BioinformaticsEssays: (Each carries 15Marks)1. Describe the methods of Introducing recombinant DNA into Host cells.2. Describe cDNA synthesis & its applications3. Describe the different methods of recombinant DNA into host cells4. Describe genetic engineering & its applications5. Describe the isolation of DNA & synthesis of recombinant DNA6. Describe the synthesis and cloning of cDNA7. What are Restriction enzymes and write their applications?8. What is a vector? Explain the types of Vectors and its uses in Geneticengineering methods?9. What is ligation? Write the methods of Ligation in rDNA technology10. What recombinant DNA technology? Write the applications of genecloning?11. Write about the tool are using in rDNA technology?Short Questions (Each carries 4 Marks):Restriction enzymes (or) Restriction endonucleases , Cloning vectors ,Transformation (or) Transformation in bacteria, cDNA (complementaryDNA), Clones, Plasmid, Restriction mapping, RFLP, BLAST and FASTA,Proteomics and Genomics, Protein Data Bank (PDB), Lipofection, Ligationmethods, Homopolymer tailing, Cohessive end ligation, Structure of Ti-Plasmid, Shuttle vector, DNA ligase, Sanger’s method in DNA sequencingmethod, GFPVery Short Questions (Each carries 2 Marks)Palindrome, Cosmid, Probe, Bam HI, Plasmid, Restriction mapping,Genomic library, PBR322, Structural gene, BLAST, Gene bank, NCBI,SwissprotVIZIANAGRAM BRANCHOPP0SITE DARGAH, KOTA Jn, VIZIANAGRAM. Ph:9440011678M.Sc. ENTRANCE EXAMS SHORT TERM COACHING-2012*BIOTECHNOLOGY *MICROBIOLOGY*BIOCHEMISTRY *FOOD TECHNOLOGYCoaching for M.Sc. Entrance- Local & Central universities(PCU, HCU….)Secured top 10 ranks in Central & Local Universities EntranceExaminations from the past 8 yearsFor further details Contact: 9440011678, 9440715785