Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibitio...
Nitric Oxide (NO) Endothelium-derived relaxing factor (EDRF)
NADPH O 2   + Production of NO
Effector mechanisms of NO Ca 2+ NO-dependent Vasodilators -Acetylcholine -Bradykinin Blood Flow Shearing  Forces R Endothe...
Cardiovascular disease Diabetes mellitus Type I,II Cardiovascular disease Endothelial dysfunction
 
 
 
 
Methods <ul><li>Noninvasively  </li></ul><ul><li>Real time </li></ul><ul><li>in vivo  </li></ul>
Spectroscopic Determination of Percentage of HbO2 541 576 555
Imaging system. <ul><li>Liquid crystal tunable filter (LCTF) </li></ul><ul><li>Charge-coupled device detector (CCD) </li><...
Instrumentation <ul><li>A quartz tungsten halogen light source </li></ul><ul><li>(Oriel Instruments)  </li></ul><ul><li>A ...
Spectroscopic Determination of Percentage of HbO 2 <ul><li>Reference spectra of a  100% HbO 2   and  deoxy-Hb </li></ul><u...
Study Population <ul><li>9  nonsmoking healthy (5 men and 4 women) </li></ul><ul><li>Average age of  32±4  years </li></ul...
Protocol <ul><li>refrained drinking  alcohol  and beverages containing  caffeine  for ≥12 hours </li></ul>
Protocol 1. breathed room air Tem~22°C 5% dextrose in water (D5W) 1 mL/min NG-monomethyl-L-arginine (L-NMMA)  4 to 8 mol/m...
Statistical Analysis <ul><li>Differences between population means were compared by a 2-tailed </li></ul>
Results
 
 
Results
Results
Discussion <ul><li>Noninvasive techniques for providing rapid, repetitive assessment of tissue perfusion could be of consi...
Discussion <ul><li>hyperspectral imaging provides representations of the spatial distribution of a molecular species at a ...
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Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique

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Journal Club 3 : Nitric Oxide Sensors

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  • การหาระยะเวลาของการดูดซับ Nitric Oxide โดยหารให้ผ่านทางลมหายใจ โดยใช้วิธี non-invasive แบบ Image technique
  • Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique

    1. 1. Noninvasive Determination of Spatially Resolved and Time-Resolved Tissue Perfusion in Humans During Nitric Oxide Inhibition and Inhalation by Use of a Visible-Reflectance Hyperspectral Imaging Technique Karel J. Zuzak ,at all ( Circulation. 2001;104:2905-2910.)
    2. 2. Nitric Oxide (NO) Endothelium-derived relaxing factor (EDRF)
    3. 3. NADPH O 2 + Production of NO
    4. 4. Effector mechanisms of NO Ca 2+ NO-dependent Vasodilators -Acetylcholine -Bradykinin Blood Flow Shearing Forces R Endothelial Cell Smooth Muscle Cell NOS GC cGMP Lumen Ca 2+ K +
    5. 5. Cardiovascular disease Diabetes mellitus Type I,II Cardiovascular disease Endothelial dysfunction
    6. 10. Methods <ul><li>Noninvasively </li></ul><ul><li>Real time </li></ul><ul><li>in vivo </li></ul>
    7. 11. Spectroscopic Determination of Percentage of HbO2 541 576 555
    8. 12. Imaging system. <ul><li>Liquid crystal tunable filter (LCTF) </li></ul><ul><li>Charge-coupled device detector (CCD) </li></ul><ul><li>Analog-to-digital converter (ADC) </li></ul>
    9. 13. Instrumentation <ul><li>A quartz tungsten halogen light source </li></ul><ul><li>(Oriel Instruments) </li></ul><ul><li>A mirror ,Liquid crystal tunable filter(LCTF) </li></ul><ul><li>(Cambridge Research and Instrumentation) </li></ul><ul><li>Lens (Nikon) </li></ul><ul><li>CCD detector (Roper Scientific) </li></ul><ul><li>GX1P computer (Dell) </li></ul>
    10. 14. Spectroscopic Determination of Percentage of HbO 2 <ul><li>Reference spectra of a 100% HbO 2 and deoxy-Hb </li></ul><ul><ul><li>Standard methods using blood collected from a healthy, nonsmoking </li></ul></ul><ul><ul><li>7 mL of hemolysates was mixed with 30 mg of sodium dithionite </li></ul></ul><ul><ul><li>The HbO 2 spectrum displays distinct peaks at 541 and 576 nm </li></ul></ul><ul><ul><li>Deoxy-Hb displays a single peak at 555 nm </li></ul></ul>
    11. 15. Study Population <ul><li>9 nonsmoking healthy (5 men and 4 women) </li></ul><ul><li>Average age of 32±4 years </li></ul><ul><li>Clinical history, physical examination </li></ul><ul><li>ECG, and routine laboratory analyses </li></ul><ul><li>No evidence of cardiovascular disease </li></ul>
    12. 16. Protocol <ul><li>refrained drinking alcohol and beverages containing caffeine for ≥12 hours </li></ul>
    13. 17. Protocol 1. breathed room air Tem~22°C 5% dextrose in water (D5W) 1 mL/min NG-monomethyl-L-arginine (L-NMMA) 4 to 8 mol/min 20 min 2. NO 80 ppm blood flow was measured by venous occlusion plethysmography(Hokanson) NOS inhibitor during which time hyperspectral images were collected
    14. 18. Statistical Analysis <ul><li>Differences between population means were compared by a 2-tailed </li></ul>
    15. 19. Results
    16. 22. Results
    17. 23. Results
    18. 24. Discussion <ul><li>Noninvasive techniques for providing rapid, repetitive assessment of tissue perfusion could be of considerable utility in the management of a variety of vascular diseases. In addition to assessing and quantifying the concentration of a specific chemical species, such as HbO 2 and deoxy-Hb, </li></ul>
    19. 25. Discussion <ul><li>hyperspectral imaging provides representations of the spatial distribution of a molecular species at a given time or over time. </li></ul><ul><li>Accordingly, acquisition of a time-resolved profile representing the spatial dynamics of physiologically important molecules indicative of tissue perfusion could prove useful in assessing the efficacy of a given therapeutic intervention on vascular disease. </li></ul>
    20. 26. Thank You

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