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Influence of Silicon on Cadmium uptake in durum wheat cultivars

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These slides narrates a research project about effect of Si on uptake of radioactive isotope Cadmium in wheat plant.

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Influence of Silicon on Cadmium uptake in durum wheat cultivars

  1. 1. Influence of Silicon on Cadmium uptake in durum wheat cultivars<br />Muhammad Arifin Sandhi<br />Masters Student, Ecology<br />Stockholm University<br />SE- 10691, Sweden<br />
  2. 2. Background<br /><ul><li>Cd is a non-essential metal for plants, present in the soil at very low concentration.
  3. 3. Occurance of Cd by industrial activity, pesticide residues, electronic waste, mining, swedge sludge deposition.
  4. 4. Cd linked with food chain and cause diseases like cancer,bone fracture,hypertension, etc.
  5. 5. In Sweden, the half of total Cd intake in the human body by potato (43%) and wheat (40%)</li></ul>(Greger & Löfstedt, 2004)<br />
  6. 6. DurumWheat (TriticumturgidumL var.)<br />Developed in Late Bronze Age(6000-7000 BC) and difference from bread wheat genome (2n=6x) where durum (2n=4x)<br />Evidence to uptake Cd in durum in US, Canada and Sweden. <br />Two common varities in Sweden, Helidur (low accumulator)and Grandur(high accumulator).<br />(Greger & Löfstedt, 2004)<br />
  7. 7. Silicone<br />Silicon (Si) helps the plant to recover them from biotic (pest and pathogen) and abiotic stress (heavy metal).<br />Some cereal crops under Gramineae and Cyperaceae can uptake high amount of Si.<br />Si helps initiation of tolerance mechanism against toxic metals.<br />(Gong et al., 2008)<br />
  8. 8. Questions?<br />Influence of Silicon<br />on<br /><ul><li>Cd uptake in respect of time.
  9. 9. Net uptake of Cd in root regions.
  10. 10. Translocation percentage between two durum wheat cv.</li></li></ul><li>Materials<br /><ul><li>Durum wheat cultivars; helidur and grandur
  11. 11. Cd isotope (42 nM) and half life 453 days.
  12. 12. Potassium Silicate</li></li></ul><li>Methods<br />The wheat seedlings are cultivated in climate chamber on hydroponics medium for 2 weeks.<br />There are four treatments of Si (0, 0.5, 2, 5 nM) with 3 replications and each replicate has 4 plants in each.<br />After addition of Cd, collect the solution from medium in respect of time (0 min, 12 min,24 min, 36 min, 1 h, 4 h, 20 h, 48 h) and analysed in Scintillation machine (DPM) with addition of Emulsifier.<br />After 48 hours plant biomass collected, wet digestated and analysed in scintillation machine.<br />
  13. 13. Result<br />Cd Uptake<br />
  14. 14. Results<br />Cd Uptake/minute<br />
  15. 15. Results<br />Net Uptake Cd by roots<br />
  16. 16. Results<br />Translocation Percentage<br />
  17. 17. Discussions<br /><ul><li>Uptake is higher in grandur cv, but uptake rate/min is faster in helidur cv with increase of Si treatments.It is probably binding of available Cd in the solution with root cell proteins and make it inactive complex molecules .
  18. 18. The net uptake of Cd in root decreased with increase of Si application. This decrease trend is more higher in helidur than grandur.This findings suggested that higher dose of Si application reduce Cd uptake in the roots of helidur.
  19. 19. Si application reduce the translocation percentage of Cd in shoots of helidur than grandur.The common trend of metal sink root>shoot>grain. As Si deposited in the leaf area of wheat, so plant leaf can avoid and reduce the Cd in the shoot with help of Si. </li></li></ul><li>Conclusions<br />Research needs for helidur as Si decrease the accumulation of Cd in both root and translocation in shoot.<br />Carry out research in field level because hydroponics medium can cause higher stress than field condition. <br />
  20. 20. Acknowledgement<br />Docent Maria Greger<br />&<br />FL Tommy Landberg<br />
  21. 21. Questions?<br />
  22. 22. Tack såmycket!<br />

These slides narrates a research project about effect of Si on uptake of radioactive isotope Cadmium in wheat plant.

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