Pesticides Degradation Through Microorganisms (Biodegradtion)
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Pesticides Degradation Through Microorganisms (Biodegradtion)
- 2. Group Members •Adan Fatima Javed •Sehrish Shoukat •Zunaira Khalil •Basharat Khan •Syed Haider Ali •Salimullah Khan Ayubi
- 4. Pesticides Pesticides are substances meant for preventing, destroying or mitigating any pest.
- 5. Types of pesticides/Classes breakup 2% Insecticides 9% Herbicides 14% Fungicides 75% Acricides
- 6. Why Pesticides are used? • Population pressure • Food security • Higher yield
- 7. • Province wise distribution KPK 2% Sindh 8% Punjab 90%
- 8. Toxicity potential of Pesticides Direct impact on humans • Adversely impact on Central Nervous System (CNS) • Toxic
- 9. Fate of Pesticide in Environment
- 10. Profenofos
- 11. Selected Pesticide-Profenofos • Widely used in Pakistan • Hazardous to health
- 12. Reasons • Among top ten used pesticides in Pakistan – Used on cotton – Commonly found as groundwater contaminants – More effective
- 13. Biodegradation • Microorganisms • Use compound as carbon and energy source
- 14. Methodology
- 15. Methodology Steps Sampling Enrichment Incubation Spreading Plate Count Isolation Acclimatization Serial Dilutions Characterization
- 16. Media Mineral Salt Media (MSM) Use Distilled Water For 100 ml of media (0.15g/100 ml)
- 17. Autoclave •Media •Glass wares e.g petri plates etc….
- 19. Acclimatization 1 ml Inoculum Media 100 ml 100 ml Concentration of profenofos control 50 ppm 1 ml 100 ml 100 ppm 1 ml 100 ml 150 ppm
- 22. Incubation For 2 days (48 hours) at 37 oC.
- 23. Plate Count
- 24. Characterization •Morphological Characterization Colony Morphology (Microscopy) Cell Morphology (Through staining) •Physiological Characterization •Biochemical Characterization •Molecular Characterization
- 25. Conclusion •Specific growth of pesticide degrading microorganisms •Planning for isolation and Characterization in the next step
- 26. Experience •Very good experience •Applications of Microbiology in Environmental Engineering •Would like to continue the research in the next semesters
- 27. Facilities in IESE Microbiology Lab •Sterile Environment •Well equipped •Very cooperative and trained Staff •Students/Researchers are always welcomed
- 28. Suggestions • Separate lab for Bioremediation • HEPA Filters
- 29. Acknowledgement Professor Dr. Imran Hashmi Miss Arish Naseem