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Molecular Biology Lecture


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Molecular Biology Lecture

  1. 1. Molecular Biology
  2. 2. Fig. 10-1a Head DNA Tail Tail fiber
  3. 3. Fig. 10-1b Radioactive Empty Phage protein Radioactivity protein shell in liquid Bacterium Phage DNA DNA Batch 1 Radioactive protein Centrifuge Pellet 2 Agitate in a blender to 3 Centrifuge the mixture 4 Measure the 1 Mix radioactively separate phages so bacteria form a labeled phages with radioactivity in outside the bacteria pellet at the bottom of bacteria. The phages the pellet and from the cells and the test tube. infect the bacterial cells. the liquid. their contents. Radioactive DNA Batch 2 Radioactive DNA Centrifuge Radioactivity in pellet Pellet
  4. 4. DNA Structure
  5. 5. Fig. 10-3b
  6. 6. Fig. 10-3a
  7. 7. Fig. 10-2aa Nitrogenous base (A, G, C, or T) Phosphate group Thymine (T) Sugar (deoxyribose)
  8. 8. Fig. 10-2b Thymine (T) Cytosine (C) Guanine (G) Adenine (A) Pyrimidines Purines
  9. 9. Fig. 10-2c Nitrogenous base (A, G, C, or U) Phosphate group Uracil (U) Sugar (ribose)
  10. 10. Fig. 10-3da Base pair Ribbon model
  11. 11. Fig. 10-4a-3 Nucleotides Parental Both parental Two identical molecule strands serve daughter of DNA as templates molecules of DNA
  12. 12. DNA replication occurs during S phase of the Cell Cycle
  13. 13. Fig. 10-4b
  14. 14. Fig. 10-5b 3 end 5 end P 5 2 4 3 3 1 1 4 2 5 P P P P P P P 5 end 3 end
  15. 15. Fig. 10-5a Parental strand Origin of replication Daughter strand Bubble Two daughter DNA molecules
  16. 16. Fig. 10-5c DNA polymerase 3 molecule 5 Daughter strand synthesized Parental DNA 5 continuously 3 Daughter strand 3 synthesized 5 in pieces 5 3 DNA ligase Overall direction of replication
  17. 17. Protein Synthesis occurs during G0, G1 and G2 of the cell cycle
  18. 18. Fig. 10-6a-3 DNA Transcription RNA Nucleus Cytoplasm Translation Protein
  19. 19. Fig. 10-7a DNA strand Transcription RNA Codon Translation Polypeptide Amino acid
  20. 20. Fig. 10-9b RNA polymerase DNA of gene Promoter Terminator DNA DNA 1 Initiation Area shown 2 in Figure 10.9A Elongation Growing 3 RNA Termination Completed RNA RNA polymerase
  21. 21. Fig. 10-8a Second base Third base First base
  22. 22. Fig. 10-12a Growing tRNA polypeptide molecules Large subunit mRNA Small subunit
  23. 23. Fig. 10-12c Next amino acid to be added to polypeptide Growing polypeptide tRNA mRNA Codons
  24. 24. Fig. 10-14-4 Amino acid Polypeptide A site P site Anticodon mRNA Codons 1 Codon recognition mRNA movement Stop codon 2 Peptide bond formation New peptide bond 3 Translocation
  25. 25. Mutations
  26. 26. Fig. 10-16a Normal hemoglobin DNA Mutant hemoglobin DNA mRNA mRNA Sickle-cell hemoglobin Normal hemoglobin Glu Val
  27. 27. Plasmodium falciparum
  28. 28. Fig. 10-16b Normal gene mRNA Protein Lys Met Phe Ala Gly Base substitution Lys Met Phe Ala Ser Base deletion Missing Lys Met Leu His Ala