D2d Hts

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discovery 2 diagnostics presentation 2008

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D2d Hts

  1. 1. Image Processing Techniques for Automate Sample Preparation using Piezoelectric Liquid Handling Robotics Richard Rouse HTS Resources, LLC Discovery 2 Diagnostics 2008 HTS Resources, LLC
  2. 2. How our business is set up GeSiM GmbH HTS Resources, LLC Quantum Analytics, LLC Dresden, Germany San Diego, CA Foster City, CA Developer and North American Equipment Support and North American Sales Manufacturer Application and Distribution Development Discovery 2 Diagnostics 2008 HTS Resources, LLC
  3. 3. Piezo dispensing basics Piezo Squeezes Piezo Pushes Around Against Advantages ● Non contact dispensing ● Diverse dispense patterning ● Multiple dispensing from a single aspiration ● Amendable for implementing quality control ● Flexible platform to address requirements involved with assay validation Discovery 2 Diagnostics 2008 HTS Resources, LLC
  4. 4. Principle of piezo dispensing operation Discovery 2 Diagnostics 2008 HTS Resources, LLC
  5. 5. Mass spectrometry application Sequenom SpectroJET MALDI matrix spotting Sequencing through mass spectrometry Discovery 2 Diagnostics 2008 HTS Resources, LLC
  6. 6. Nano-Plotters now Discovery 2 Diagnostics 2008 HTS Resources, LLC
  7. 7. Open programming architecture Nano-Plotter Language = .npl Program editor Built in functions Compiling and running Discovery 2 Diagnostics 2008 HTS Resources, LLC
  8. 8. Open programming architecture Access to serial port programming through 2 NP available serial ports Discovery 2 Diagnostics 2008 HTS Resources, LLC
  9. 9. Open programming architecture Discovery 2 Diagnostics 2008 Workplate objects HTS Resources, LLC
  10. 10. Open programming architecture Running .npl programs in graphical environment Selecting a program to run Edit variable values in run time Discovery 2 Diagnostics 2008 HTS Resources, LLC
  11. 11. Open programming architecture Running programs in simulation Discovery 2 Diagnostics 2008 HTS Resources, LLC
  12. 12. Integration of optical components Video Microscope Stroboscope Head camera Discovery 2 Diagnostics 2008 HTS Resources, LLC
  13. 13. Accessing stroboscope data to control dispensing function StrobCheck(tipList: integer):record; begin Stroboscope(mode=1, tip = tipId); TimerDelay(t = 2); Grab(DROP); ok[tip] = FindDots(DROP); record.ydist=GetGrabberValue(DROP, Y_dist); record.drops=GetGrabberValue(DROP, Drops); StrobCheck = record; end; Discovery 2 Diagnostics 2008 HTS Resources, LLC
  14. 14. Using stroboscope to aspirate powders for dispensing The empty powder pipette approached closely over the powder reservoir Powder aspirated by means of vacuum impulse Discovery 2 Diagnostics 2008 HTS Resources, LLC
  15. 15. Elements of CamViewConfig Define search areas Discovery 2 Diagnostics 2008 HTS Resources, LLC
  16. 16. Elements of CamViewConfig Use filters to adjust contrast Discovery 2 Diagnostics 2008 HTS Resources, LLC
  17. 17. Elements of CamViewConfig Scale to size Discovery 2 Diagnostics 2008 HTS Resources, LLC
  18. 18. Elements of CamViewConfig Find spots Discovery 2 Diagnostics 2008 HTS Resources, LLC
  19. 19. Access CamViewConfig through NPL code Find spot positions #include quot;../lib/GrabberTools.incquot; var p: tPoint; var i, n: integer; begin ShowGrabWindow(); Grab(TPLT); FindDots(TPLT); n = GetDotCount( finderId=TPLT); for i = 1 to n do begin p = GetFoundedDot( finderId=TPLT, dotNumber=i); Writeln(p.x, p.y); end; end. Discovery 2 Diagnostics 2008 HTS Resources, LLC
  20. 20. Manual definition of spot points in the frame grabber window Definition of manual grabber points on nanowells. Discovery 2 Diagnostics 2008 HTS Resources, LLC

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