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  1. 1. Amander Clark Ph.D. Curriculum Vitae CURRENT POSITION 2006 Assistant Professor University of California, Los Angeles Department of Molecular Cell and Developmental Biology Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research BSRB II, Box 951606 Los Angeles, CA 90095 PREVIOUS POSITION 2002- 2006 Assistant Research Geneticist (senior postdoc) University of California, San Francisco Department of Obstetrics and Gynecology and Reproductive Sciences University of California, San Francisco. San Francisco, CA, USA. Mentor: Associate Professor Renee Reijo Pera Ph.D. EDUCATION AND TRAINING 1999-2002 Postdoctoral Associate, Department of Molecular and Human Genetics Baylor College of Medicine, Houston, Texas, USA. Mentor: Associate Professor Monica Justice Ph.D. 1995-1999 Ph.D., Anatomy and Cell Biology University of Melbourne, Parkville, Victoria, Australia. Mentor: Professor John Bertram Ph.D. 1995 B.Sc. (Honors), Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Victoria, Australia. Mentor: Professor John Bertram Ph.D. 1991-1994 B.Sc., Major in Pathology and Minored in Biochemistry, University of Melbourne, Parkville, Victoria, Australia. HONORS AND AWARDS 2007 Lance Armstrong Foundation Young Investigator Award 2007 STOP CANCER Career Development Award 2006 Faculty Diversity Award, University of California, Los Angeles 2006 Rob Taylor Award, Department of OB/GYN, Center for Reproductive Sciences 2005 Finalist in the 6th International Royan International Research Award, Tehran, Iran 2003 International Society for Stem Cell Research Young Investigator Award 1998 Travel grant for the 33rd Annual Scientific meeting of the Australian and New Zealand Society of Nephrology 1997 Keith Dixon Prize awarded by the Australian and New Zealand Society for Cell and Developmental Biology 1997 Travel grant for the XIVth International Congress of Nephrology Scientific meeting 1996 Travel grant awarded by the School of Graduate Studies, University of Melbourne, Australia
  2. 2. Page 2 of 8 Curriculum Vitae: Amander Clark Ph.D. 1996 Travel grant awarded by the Department of Anatomy and Cell Biology, University of Melbourne, Australia 1995 Australian Postgraduate Award 1995 Dean’s Honors prize for Academic Excellence, University of Melbourne, Australia 1993 Baker Medical Research Institute Undergraduate Scholarship PROFESSIONAL ACTIVITIES 2009 Associate Editor for the Journal BMC Developmental Biology 2009 Ad hoc reviewer for the Cell and Molecular Integrative Reproduction NIH study section February 2009 and October 2009 2008 Member of the Hinxton Group 2008 consortium on stem cells, ethics and law, with the objective to generate an International Statement on the ethics and policy associated with pluripotent stem cell derived gametes 2008 Organizing Committee for the Eli and Edyth Broad Center of Regenerative Medicine and Stem Cell Research Conference on Stem Cell Regulation and Reprogramming 2007 Reviewer of Stem Cell Seed Grants for Investigators in the State of Maryland 2005 Invited guest lecturer for the BioE Graduate Course on Stem Cells and Tissue Engineering, Stanford University, Palo Alto 2005 Invited Faculty lecturer for the American Society for Reproductive Medicine Postgraduate Course, Montreal, Canada 2005 Invited Faculty to teach in the Frontiers in Human Embryonic Stem Cell Research Advanced Training Course, Stanford University, Palo Alto 2004 Organizing committee for the Human Frontiers in Science US-UK symposium, sponsored by the National Academy of Sciences and the Royal Academy of Sciences, Hinxton Hall, Cambridge 2003- Member of the International Society for Stem Cell Research 2002-2005 Member of Women in Endocrinology 2000-2002 Member of The American Society of Nephrology 1995-1998 Member of The Australian and New Zealand Society of Nephrology 1996-1998 Australian Society for Cell and Developmental Biology INVITED PAPERS, LECTURES, PRESENTATIONS 2010 Invited Speaker and session chair, UCSC Developmental Biology meeting. Title of talk: ‘Epigenetic reprogramming of germ line stem cells’ Santa Cruz, CA 2010 Keynote Speaker, UCSF Reproductive Science Day Retreat. Title of talk ‘Using pluripotent stem cells to uncover molecular pathways critical to Reproductive Biology’ San Francisco, CA 2010 Invited Speaker, Monash University, Department of Anatomy and Cell Biology Seminar Series. Title of talk: ‘Identifying mechanisms that regulate fetal germ cell development’ Clayton, Australia 2010 Invited Speaker, Brasel Basic Science seminar series, Harbor UCLA. Title of Talk: ‘Pluripotent stem cells in Reproductive Biology, Torrance, CA 2010 Invited Speaker, UCLA Embryology Club. Title of talk: ‘Characterizing the emergence of fetal germ line using pluripotent stem cells’ Los Angeles, CA 2010 Invited Speaker, 1st Broad Stem Cell Conference. Title of talk: ‘Using pluripotent stem cells to understand germ cell development’ Asilomar, CA
  3. 3. Page 3 of 8 Curriculum Vitae: Amander Clark Ph.D. 2010 Invited Speaker, American Society for Andrology Annual Meeting. Title of talk: ‘The therapeutic potential of germ stem cells’ Houston TX 2010 Invited Speaker, Southern California Germ Cell Group, Title of talk: ‘Defining the molecular events in human fetal germ cell development’ Irvine, CA 2010 Invited Speaker, 2nd Stanford University Reproductive Science Day. Title of talk: ‘Formation of the human fetal germ line’ Palo Alto, CA 2009 Invited Speaker, 3rd Annual NIH-NIGMS Workshop: Human Embryonic Stem Cell Research: Recent Progress and Future Directions of NIGMS Grantees at the NIH. Title of talk: ‘Epigenetic differences in embryonic stem cells and embryonic stem cell derived germ cells’ Bethesda, MD 2009 Invited Speaker, UCLA Engineering Department Seminar Series. Title of talk: ‘Generating Germ Cells in a dish from pluripotent stem cells’ Los Angeles, CA 2009 Invited Speaker, University of Southern California Stem Cell Seminar series. Title of talk: ‘Pluripotent Stem Cells in Reproductive Medicine’ Los Angeles, CA 2009 Invited Speaker, 1st Stanford University Reproductive Science Day Symposium. Title of Talk: ‘Epigenetic reprogramming of primordial germ cells derived from hESC and hIPS cells’ Palo Alto, CA 2009 Invited Speaker, 9th International Congress of Andrology. Title of talk: ‘Epigenetic reprogramming of primordial germ cells derived from hESC and hIPS cells’ Barcelona, Spain 2008 Invited Speaker, 2nd Annual meeting of the Stem Cells Development and Disease Conference. Title of talk: ‘Epigenetic reprogramming of primordial germ cells derived from hESC and hIPS cells’ Amsterdam, Netherlands 2008 Invited Speaker, Cedars Sinai Medical Center, Stem Cell Seminar series. Title of talk: ‘Modeling development and disease using human embryonic stem cells’ Los Angeles, CA 2008 Invited Speaker, Children’s Hospital Los Angeles Seminar Series. Title of talk: ‘Modeling development and disease using human embryonic stem cells’ Los Angeles, CA 2007 Invited Speaker, Las Donas Morning at UCLA to discuss topics of interest relative to society. Title of talk: ‘Human Embryonic Stem Cells in Basic Research’ Los Angeles, CA 2007 Invited Speaker, Harbor UCLA Pathology Grand Rounds. Title of talk: ‘Human Embryonic Stem Cells, differentiation and cancer’, Torrance, CA 2006 Invited Speaker, International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Title of talk: ‘Establishment and Differentiation of Human Embryonic Stem Cell-Derived Germ Cells’ New Delhi, India 2005 Invited Speaker, 9th Annual Reproductive Research Day, Center for Women’s Health and Reproduction, Stanford University School of Medicine. Title of Presentation: ‘The Common Genetics of Human Embryonic Stem Cells and Germ Cells’ Palo Alto, CA 2004 Invited Speaker, Research Seminar Series, Center for Reproductive Sciences, OB/ GYN, UCSF. Title of talk: ‘The Genetics of Embryonic Stem Cells, Germ Cells and Cell Fate Decisions’ San Francisco, CA
  4. 4. Page 4 of 8 Curriculum Vitae: Amander Clark Ph.D. 2003 Invited Speaker, Monash University, Department of Anatomy and Cell Biology, Sponsored by the Australian Stem Cell Center. Title of talk: ‘Differentiation of Germ Cells from Embryonic Stem Cells in vitro’ Clayton, Australia 2003 Invited Speaker, UCSF School of Medicine, Department of Stomatology and OB/ GYN Research Seminar Series. Title of talk: ‘Germ cells and the Genetic Balance between Pluripotency and Differentiation’ San Francisco, CA 2003 Invited Speaker, Stanford OB/GYN Seminar Series. Title of talk: ‘Human Embryonic Stem Cells as a Model for Germ Cell Differentiation.’ Palo Alto, CA 2003 Invited Speaker, 1st Annual Meeting for the International Society for Stem Cell Research. Title of talk: ‘Chromosome 12p13.1 a Hot-Spot for Teratocarcinoma, Encodes Three Pluripotency-Associated Genes with Remarkable Evolutionary Divergence from Humans to Mice.’ Washington D.C. 2002 Invited Speaker, Center for Reproductive Sciences, OB/GYN, UCSF. Title of talk: ‘Germ Cell Enriched genes: Functional Roles in Differentiation and Pluripotency’ San Francisco, CA 1999 Invited Review, American Journal of Physiology. Title of paper: The Molecular Regulation of Nephron Endowment 1997 Invited Speaker, Proceedings of the XIV International Congress of Nephrology, Sydney Australia 1997 Invited Speaker, Proceedings of the 16th Annual meeting of the Australian and New Zealand Society of Cell and Developmental Biology, Melbourne, Australia UNIVERSITY SERVICE 2009 Current good manufacturing practice (cGMP) subcommittee for the derivation of pluripotent stem cell lines compatible with clinical applications 2009 Selection Committee for admission of outstanding CSST students into the ACCESS program at UCLA 2009 Working group to place top Chinese Students accepted into the Cross- Disciplinary Scholars in Science and Technology (CSST) program in laboratories at UCLA 2006 Member of the UCLA, Eli and Edyth Broad Center for Regenerative Medicine and Stem Cell Research Working Group 2006 Member of Subcommittee for Human Embryo and ESC Derivation Suite, Eli and Edyth Broad Center for Regenerative Medicine and Stem Cell Research, UCLA 2005-2006 Founding member of the Coalition of Young Stem Cell Investigators, UCSF 2004-2006 Organizer of the UCSF Center for Reproductive Sciences Embryonic Stem Cell Discussion group 2004 -2006 Chair of the Obstetrics Gynecology and Reproductive Sciences Viral Vector Core facility 2003-2004 Organizer for Mouse Genetics Journal Club, UCSF 1998-2000 Member of the Post-Doctoral Association Executive Committee, Baylor College of Medicine 1997 University of Melbourne Postgraduate Association Representative for the Department of Anatomy and Cell Biology 1997 Graduate School Representative on the Academic Programs Committee, University of Melbourne 1995 Sexual Harassment Officer for St Mary’s College, University of Melbourne
  5. 5. Page 5 of 8 Curriculum Vitae: Amander Clark Ph.D. PUBLIC VOLUNTARY SERVICE 2008 Created a Video promotion for the California Institute for Regenerative Medicine called ’Making Embryonic Stem Cell Lines’ 2007 Hosting high school students to visit my laboratory and learn about the workings of a human embryonic stem cell lab. SCIENTIFIC CONSULTANT 2004: Scientific Advisor for the crime fiction novel ‘In a Family Way’, authored by Jay Calder, 2005 Chronicle Books. CURRENT FUNDING 1) NIH P01GM081621 Amander Clark, Principle Investigator of project 2 09/01/08 – 08/31/13 Jerome Zack, Principle Investigator $9,370,688 direct Epigenetic Regulation of Germ Cell Derivation from hESCs $1,155,000 this project 2) California Institute for Regenerative Medicine Amander Clark, Principle Investigator 10/01/08-09/30/11 Generation of Pluripotent cell lines from human embryos $900,000 direct 3) NIH R01HD058047 Amander Clark, Principle Investigator 02/01/09-01/31/14 Derivation and Characterization of Germ Cells from Embryonic Stem $1,075,000 direct Cells 5) California Institute for Regenerative Medicine Amander Clark, Co Investigator 07/01/2010-06/30/213 Yong Kim, Principle Investigator $1,050,000 direct Novel Mechanism in Self-Renewal/Differentiation of Human Embryonic Stem Cells Completed funding 2009-2010 Academic Senate Role: Principle Investigator 2009-2009 Cancer Research Coordinating Committee Role: Principle Investigator 2007-2009 Lance Armstrong Foundation Role: Principle Investigator 2007-2008 UCLA JCCC Collaborative Project in Cancer Role: Co-Principle Investigator 2007-2008 UCLA ICMIC Developmental Project Role: Project Leader 2006: UCLA Faculty Diversity Career Development Award Role: Principle Investigator 2004-2006 American Cell Therapy Research Foundation Role: Co-Investigator 2003-2004 Developmental and Stem Cell Biology Grant, UCSF Role: Co-Investigator 2003-2004 Academic Senate Young Investigator Grant, UCSF
  6. 6. Page 6 of 8 Curriculum Vitae: Amander Clark Ph.D. Role: Principal Investigator 2002-2003 Research Evaluation and Allocation Committee Grant, UCSF Role: Principal Investigator 2000-2001 Lalor Foundation Postdoctoral fellowship (renewal). 1999-2000 Lalor Foundation Postdoctoral fellowship. PEER REVIEW PUBLICATIONS Original Research Articles: 1. Tchieu, J., Kuoy, E., Chin, MH., Trinh, H., Patterson, M., Sherman, SP., Aimiuwu, O., Lindgren, A., Hakimian, S., Zack, JA., Clark, AT., Pyle, AD., Lowry, WE., Plath, K (2010) Female Human iPSCs Retainan Inactive X Chromosome. Cell Stem Cell (Epub ahead of print). 2. Biancotti, JC., Narwani, K., Buehler, N., Mandefro, B., Golan-Lev, T., Yanuka, O., Clark, AT., Hill, D., Benvenisty, N., Lavon, N (2010) Human Embryonic Stem Cells as Models for Aneuploid Chromosomal Syndromes. Stem Cells (Epub ahead of print). 3. Ambartsumyan. G., Gill, RK., Diaz Perez, S., Conway, D., Vincent, J., Dalal, Y., Clark, AT (2010) Centromere Protein A dynamics in human pluripotent stem cell self renewal, differentiation and DNA damage. Human Molecular Genetics (Epub ahead of print). 4. Chodavarapu RK., Feng, S., Bernatavichute, YV., Chen, PY., Stroud, H.,Yu, Y., Hetzel, JA., Kuo, F., Kim, J., Cokus, SJ., Casero, D., Bernal, M., Huijser, P., Clark, AT., Kra¨mer, U., Merchant, SS., Zhang, X., Jacobsem, SE., Pellegrini, M (2010) Relationship between nucleosome positioning and DNA methylation. Nature (Epub ahead of print). 5. Kamei, K., Ohashi, M., Gschweng, E., Ho, Q., Suh, J., Tang, J., Yu, Z., Clark, AT., Pyle, AD., Teitell, MA., Lee, KB., Witte, ON., Tseng, H-R (2010) Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions. Lab on a chip 10: 1113-1119 6. Chin, MH., Mason, MJ., Xie, W., Volinia, S., Singer, M., Peterson, C., Ambartsumyan, G., Aimiuwu, O., Richter, L., Zhang, J., Khvorostov, I., Ott, V., Grunstein, M., Lavon, N., Benvenisty, N., Croce, CM., Clark, AT., Baxter, T., Pyle, AD., Teitell, MA., Pelegrini, M., Plath, K., Lowry, WE (2009) Induced Pluripotent Stem Cells and Embryonic Stem Cells Are Distinguished by Gene Expression Signatures. Cell Stem Cell 5: 111-123 7. Kim, Y., Deshpande, A., Dai, Y., Kim, JJ., Lindgren, A., Conway, A., Clark, AT., Wong, DT (2009) Cyclin-dependent kinase 2-associating protein 1 Commits Murine Embryonic Stem Cell Differentiation through Retinoblastoma Protein Regulation. Journal of Biological Chemistry. Epub June 8. Xie, W., Song, C., Young, NL., Sperling, AS., Xu, F., Sridharan, R., Conway, AE., Garcia, BA., Plath, K., Clark, AT*., Grunstein, M* (2009) Histone h3 lysine 56 acetylation is linked to the core transcriptional network in human embryonic stem cells. Molecular Cell 33: 417-427 (* co-corresponding author) 9. Karumbayaram, S., Novitch, B., Patterson, M., Umbach, J., Richter, L., Lindgren, A., Conway, AE., Clark, AT., Goldman, S., Plath, KP., Kornblum, H., Lowry, WE (2009) Directed differentiation of human induced-pluripotent stem cells (iPS) generates active motor neurons. Stem Cells 27:806-811 10. Park, TS., Galic, Z., Lindgren, A., Conway, AE., Van Handel, BJ., Magnusson, M., Richter, L., Teitell, MA., Mikkola, HKA., Lowry, WE., Plath, K., Clark, AT (2009) Derivation of Primordial germ cells from Human embryonic and induced pluripotent stem Cells is significantly improved by co-culture with human fetal gonadal cells. Stem Cells 27: 783-795
  7. 7. Page 7 of 8 Curriculum Vitae: Amander Clark Ph.D. 11. Conway, AE., Lindgren, A., Galic, Z., Pyle, D., Wu, H., Zack, J., Pelligrini, M., Teitell, MA., Clark, AT (2009) A Self-Renewal Program Controls the Expansion of Genetically Unstable Cancer Stem Cells in Pluripotent Stem Cell-Derived Tumors. Stem Cells 27:18-28 12. Hao, W., Kim, K., Mehta, K., Paxia, S., Sundstrom, A., Anantharamen, T., Ali, K., Doan, T., Ghosh, J., Pyle, A., Clark, AT., Lowry, WE., Fan, G., Baxter, T., Mishra, B., Sun, Y., Teitell, M (2008) Copy number variant analysis of human embryonic stem cells. Stem Cells 26:1484-1489 13. Lowry, WE., Richter, L,. Yachechko, R., Pyle, AD., Tshieu, J., Sridharan, R., Clark, AT., Plath, K (2008) Generation of human induced pluripotent stem cells from dermal fibroblasts, PNAS Feb 26, 105(8):2883-2888 14. Bostick, M., Kim, JK., Esteve, PO, Clark, AT, Pradhan, S and Jacobson, SE (2007) UHRF1 plays a role in maintaining DNA methylation in mammalian cells Science 317:1760-1764 15. Fox, M., Clark, AT, Majdoubi, ME., Vigne, JL., Griswald, MD., Urano, J., Weiner, RI., Reijo Pera, RA (2007) Distinct RNA and Protein Complexes Distinguish Human and Mouse Embryonic Stem Cells and Germ Cells Dev Biol 302:417-431 16. Kee, K., Gonsalves, JM., Clark, AT., Reijo Pera, RA (2006) Bone Morphogenetic Proteins Induce Germ Cell Differentiation from Human Embryonic Stem Cells. Stem Cells and Development 15; 831-837 17. Ezeh, U, Turek, PJ, Reijo Pera, RA, Clark, AT (2005) Human Embryonic Stem Cell Genes NANOG, STELLAR and GDF3 are expressed in both Seminoma and Breast Cancer Cancer 104: 2255-2265 18. Clark, A.T. Bodnar, M., Fox, M., Rodriguez, RT., Abeyta, MJ., Firpo, MT., Reijo Pera, RA (2004) Spontaneous Differentiation of Germ Cells from Human Embryonic Stem Cells in vitro. Human Molecular Genetics 13(7):727-739. 19. Abeyta, M.J., Clark, AT., Rodriguez, RT. Bodnar, M, Reijo-Pera, RA. Firpo, MT (2004) Global Gene Expression in Human Embryonic Stem Cell Lines. Human Molecular Genetics 13(6): 601-8 20. Clark, AT., Rodriguez, R.T., Abeyta, MJ., Firpo, MT., Reijo-Pera, RA (2004) Chromosome 12p13.1, a hot-spot for Teratocarcinoma, Encodes Three Genes Expressed in Pluripotent Cells with Remarkable Evolutionary Divergence from Humans to Mice Stem Cells 22(2) 169-179 21. Clark, AT., Firozi, K., Justice, MJ. (2004) Mutations in a Novel Locus on Mouse Chromosome 11 Resulting in Male Infertility Associated with Defects in Microtubule Assembly and Sperm Tail Function Biology of Reproduction 70:5; 1317-1324 22. Kile BT*., Hentges KE*., Clark AT*., Nakamura H., Salinger AP., Liu B., Box N., Stockton DW., Johnson RL., Behringer RR., Bradley A., Justice M.J (2003) Functional Genetic Analysis of Mouse Chromosome 11. Nature. 4;425(6953):81-6. *denotes equal contribution. 23. Clark, A.T., Young, R., Bertram, J.F. (2001). In vitro and Localization Studies on the Role of TGF-β1 in Rat Metanephric Development. Kidney International. 59:1641-1653. 24. Martinez, G., Loveland, KL., Clark, AT., Dziadek, M., and Bertram, JF. (2001) Expression of BMP Receptors in the Developing Mouse Metanephros. Experimental Nephrology. 9:372-379. 25. Joyce IM., Clark AT., Pendola, FL., Eppig JJ (2000) Comparison of Recombinant Growth Differentiation Factor-9 and Oocyte Regulation of Kit Ligand Messenger
  8. 8. Page 8 of 8 Curriculum Vitae: Amander Clark Ph.D. Ribonucleic Acid Expression in Mouse Ovarian Follicles. Biology of Reproduction. 63(6):1669-1675 26. Elvin JA,. Clark AT., Wang, P., Wolfman NM., and Matzuk MM (1999) Paracrine Actions of Growth Differentiation Factor-9 in the Mammalian Ovary. Molecular Endocrinology, 13: 1035-1048 27. Clark, AT., and Bertram, JF (1999) The Molecular Regulation of Nephron Endowment. American Journal of Physiology 276: F485-F497 28. Clark, AT., Ford, M., Nurcombe, V., Alcorn, D., Key, B., Puche, A., and Bertram, F (1995). Expression of Transforming Growth Factor-β Type II Receptor mRNA in Embryonic and Adult Rat Kidney. Nephrology, 1: 547-553 Book Chapters: 1. Lindgren, A., Gkountela, S., and Clark, AT. Pluripotent stem cells in reproductive medicine: Formation of the human germ line in vitro. In ‘Stem Cells and Regenerative Medicine’ ed. Appasani, K Springer/Humana Press (Accepted) 2. Fox, M., Reijo Pera, RA,. and Clark, AT The DAZ gene family and human germ cell development from embryonic stem cells In. ‘The Sperm Cell’ ed. De Jong, C & Barratt, C Cambridge University Press, 2006, Chapter 12. 3. Clark, AT (2006) Establishment and Differentiation of Human Embryonic Stem Cell Derived Germ Cells. In ‘Gamete Biology: Emerging Frontiers in Fertility an Contraceptive Development’ ed. Gupta, SK, Koyama, K & Murray, JF Nottingham University Press 2007. Page 77-86 Review articles: 1. Clark, AT (2010) Egg-citing advances in generating primordial germ cells in the laboratory. Biology of Reproduction 82: 233-234 2. Ambartsumyan, GA., Clark, AT (2008) Aneuploidy and early human embryo development Human Molecular Genetics 17: R10-5 3. Clark, AT (2007) Testicular Cancer Stem Cells. Stem Cell Reviews (2007); 3: 49-59 4. Clark, AT* & Pera, RA (2006) Modeling Human Germ Cell Formation with Embryonic Stem Cells. Regenerative Medicine. 1(1); 85-93 *Corresponding author 5. Clark, AT., Goldowitz D, Takahashi JS, Vitaterna MH, Siepka SM, Peters LL, Frankel WN, Carlson GA, Rossant J, Nadeau JH, Justice MJ (2004). Large-scale ENU Mutagenesis Screens in North America. Genetica. Sep;122(1):51-64. 6. Clark, AT., and Bertram, J.F. (2000). Advances in Renal Development. Current Opinion in Nephrology and Hypertension. 9:247-251.

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