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antimicrobial potential of parthenium plant extracts and study

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this is a small project done at undergrad level on pathenium plant which is a harmful weed especially in INDIA it destroys the crops to a very large extent

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antimicrobial potential of parthenium plant extracts and study

  1. 1. ANTIMICROBIAL POTENTIAL OF Parthenium PLANT EXTRACTS AND STUDY ON EFFECTS OF PARTHENIUM PLANT BIOMASS ON CROPS AND SOIL MICROFLORA By: Riddhi Karnik 1 RIDDHI KARNIK BSc RYK college nasik
  2. 2. HPT ARTS AND RYK SCIENCE COLLEGE BIOTECHNOLOGY DEPARTMENT 2 GUIDANCE FROM :ALL THE LECTURERS AND HEAD OF DEPARTMENT RIDDHI KARNIK BSc RYK college nasik
  3. 3. Last semester’s outline  Extraction of Parthenium plant parts using various solvents like ethanol & methanol  Antimicrobial activity against E.coli & Xanthomonas citri 3 Soxhlet extraction Horizontal distillation Xanthomonas citri RIDDHI KARNIK BSc RYK college nasik
  4. 4. AIMS FOR PRESENT STUDY  To study effect of Parthenium weed on crop plants.  To study the effect of Parthenium weed on soil microflora.  To study the effect of aqueous extracts of Parthenium weed leaves on saline soil’s fertility.  To observe the effect of Parthenium plant extracts on nitrogen fixing bacteria(Rhizobium) 4 RIDDHI KARNIK BSc RYK college nasik
  5. 5. Objectives  Utilisation of Parthenium weed against the plant pathogen like Xanthomonas citri and human pathogen like E.coli.  Effect of Parthenium on crops and soil  Whether Parthenium aqueous extract can impart its high salt tolerance quality to crops. 5 RIDDHI KARNIK BSc RYK college nasik
  6. 6. Introduction/Abstract  Soil is the reservoir on which most of the life forms on the earth depends. Top soil is biologically the most diverse part of the earth and it plays vital role in sustaining human welfare and assuring future agricultural productivity and stability. Introduction of weeds leads to loss of such a rich reservoir that is soil.  Parthenium or congress weed is a noxious weed which grows almost everywhere when introduced in any ecosystem. Parthenium plant being a weed is found in abundance due to its high stress tolerance and high reproductively due to its pollen grains.Microflora gets affected due to this weed. 6 RIDDHI KARNIK BSc RYK college nasik
  7. 7. Continued.  All extracts were prepared including ethanolic leaves extract, methanolic leaves extract, and aqueous leaves extract etc. Inhibitory effect was shown by the extracts against Xanthomonas citri and E.coli.  Pot assay was performed in this study which showed results considerably it was found out that even if Parthenium decreases the fertility of normal soil, it increases soils fertility when used in the form of dried biomass as well as in saline soil. When plant extracts were used to treat saline soil containing pots it showed opposite results by improving the plant growth and increased number as compared to saline control. Previously no one studied the aspect of study regarding saline soil with aqueous extracts. 7 RIDDHI KARNIK BSc RYK college nasik
  8. 8. Material and methods:  1. Plant material: Parthenium hystrophorus entire plant was uprooted from vicinity, then these plants were washed thoroughly for two to three times and dried in shade for 10-15 days. Further the plant parts were differentiated as leaves, stem, roots and flowers and powdered. These powdered parts were stored separately in air tight containers which were further used for extraction.  Bacteria :Rhizobium a nitrogen fixing bacteria 8 RIDDHI KARNIK BSc RYK college nasik
  9. 9. POT ASSAY  5 pots were taken with soil  Control: Pot containing normal soil  Wet biomass: pot containing normal soil and fresh plant biomass  Dried biomass: pot containing normal soil along with dried Parthenium plant biomass  Saline control: pot containing highly saline soil having pH-8 to 8.5  Saline soil with aqueous extract: pot containing saline soil with pH-8to 8.5 and was given a constant dosage of Parthenium leaves aqueous extract . 9 RIDDHI KARNIK BSc RYK college nasik
  10. 10. Pot assay 10 RIDDHI KARNIK BSc RYK college nasik
  11. 11.  Moong seeds were weighed 4 grams for each pot  surface sterilized  seeds were soaked in water for 5 to 6 hours  1 set was soaked in aqueous extract for same time  Then these seeds were planted  Plants were observed for entire week for their proper growth  Saline soil pot of 5th number were planted with moong seed which were given the aqueous extract regularly in diluted concentration(1 ml of aq. Extract +10ml of distilled water)  Other pot plants were watered regularly 11 RIDDHI KARNIK BSc RYK college nasik
  12. 12. 12 RIDDHI KARNIK BSc RYK college nasik
  13. 13. Results 1. Zone of inhibition in case of Rhizobium  With aqueous extracts  Plate 1st -0.2cm  Plate 2nd -0.3cm  With Methanolic extracts  1st plate-1cm,  2nd plate-0.8cm  This shows that nitrogen fixing bacteria are greatly affected thus it may result in either failure or improper yield of leguminous plants 13 RIDDHI KARNIK BSc RYK college nasik
  14. 14. 14 Control plates and Rhizobium zone of inhibition RIDDHI KARNIK BSc RYK college nasik
  15. 15. 2.Results for pot assay Sr no Specification to be observed 1st pot control 2nd pot wet biomass 3rd pot dried biomass 4th pot saline control 5th pot saline aq. Ext. 1 Length of plant in cm 14 12 16 14.2 14.8 2 Length of root in cm 4 3.4 4.3 1.2 4.2 3 No. of leaves 2 2 2 2 2 4 Length of leaves in cm 2.5 2.1 2.8 2 3.1 5 No. of plants 18 7 9 2 7 15 RIDDHI KARNIK BSc RYK college nasik
  16. 16. 16 Pots from left to right serially: saline control, saline with aqueous extract, wet biomass, dried biomass, control RIDDHI KARNIK BSc RYK college nasik
  17. 17. 17  EFFECT ON MICROFLORA OF SOIL Plates of serial dilution for obtaining the soil microflora RIDDHI KARNIK BSc RYK college nasik
  18. 18. 18 Pot no No. of colonies 1st 198 2nd 133 3rd 140 4th 72 5th 117 Pot no. No .of colonies 1st 176 2nd 111 3rd 108 4th 52 5th 97 Number of colonies were counted using colony counter For 10-7 For 10-8 Further these colonies were screened for the type of bacteria whether gram positive or gram negative RIDDHI KARNIK BSc RYK college nasik
  19. 19. 19 Pot no Type of bacteria 1st control Gram positive and gram negative 2nd wet biomass Gram positive only 3rd dried biomass Gram positive only 4th saline control Gram positive and gram negative 5th saline with aqueous extract Gram positive and gram negative Gram staining Gram negative Gram negative and positive bacteria RIDDHI KARNIK BSc RYK college nasik
  20. 20.  CONCLUSION:  Parthenium as a weed affects the growth of plants or crops in terms of their height, length of leaves and numbers.  Parthenium also affects the microflora of soil by decreasing the number of bacterial colonies.  Gram negative bacteria get inhibited in presence of the Parthenium plants to a great extent here in this study it was proved by the inhibition of Rhizobium bacteria against aqueous also the methanolic extract of leaves  On contrary when the aqueous extracts were used for treatment on saline sample it results in increased number of plants and increase in amount of microflora of soil as compared to saline control.  This study shows two aspects that in a normal soil contained with the wet Parthenium decreases the growth of moong plants as well as microflora of soil and on opposite side more number of moong plants as compared to wet biomass was observed.  Soil with high amount of salinity decreases the ability or fertility of soil and crops fail to grow, taking into consideration that Parthenium plant have high salt tolerance its aqueous extract was given to saline soil on regular basis which showed increase in number of moong plants and microflora as compared to the saline control. 20 RIDDHI KARNIK BSc RYK college nasik
  21. 21. FUTURE PROSPECTS:  Parthenium can be further proved important by the study on various plant pathogens.  At present this study can have application in enhancing the growth of flowering plants in saline soil.  In our further study we would like to see whether a plant treated with Parthenium extract is safe for consumption or not.  Also investigation needs to be done on which compound or factor results in decreased number of microbial soil content  Further study needs to be done on saline soil treated with the aqueous extract which enhanced the bacterial number to a considerable extent. 21 RIDDHI KARNIK BSc RYK college nasik
  22. 22. 6. REFERNCES  Ajay Kumar, Shailesh Joshi and Tripti Malik, ISSN 2250- 3137 ANTIMICROBIAL Potential of Parthenium hysterophorus plant extracts, www.ijlbpr.com Vol. 2, No. 3, July 2013  Seerjana maharjan, Bharat shrestha & Pramod Jha (2007),”Allelopathic effect of aqueous extract of leaves of Parthenium on germination and control of seedling and wild herbaceous species”  Yadav Neha, Saha Priyanka, Jabeen Sitara, Kumari Suruchi, Kumari Sarita, Verma Sushma Kumari, Raipat Bharti Singh and Sinha M P (2010), “Effect of Methanolic Extract of Parthenium hysterophorus L. on Haematological Parameters in Wistar Albino Rat”, The Bioscan Special Issue, Vol. 2, pp357-363. 22 RIDDHI KARNIK BSc RYK college nasik
  23. 23. Reference cont.  Lalitha p,Shivani & Rama Rao (2012), “Parthenium hysterophorus –An economical tool to increase the agricultural productivity” 23 RIDDHI KARNIK BSc RYK college nasik
  24. 24. End of presentation 24 RIDDHI KARNIK BSc RYK college nasik
  25. 25. Queries are the best ways to communicate Any questions? ? RIDDHI KARNIK BSc RYK college nasik25

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