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CTF correction
PSF - delocalization
512 x 512
central pixel
is 1.0
CTF correction
PSF - delocalization
CTF applied:
-0.5 µm
Cs = 2.7 mm,
Amp. Contrast = 0.07
B-factor: 80.0 Ų
512 x 512
central pixel
is 1.0
CTF correction
PSF - delocalization
512 x 512
central pixel
is 1.0
CTF applied:
-0.5 µm
Cs = 2.7 mm,
Amp. Contrast = 0.07
B-factor: 80.0 Ų
CTF correction
PSF - delocalization
512 x 512
central pixel
is 1.0
CTF applied:
-0.5 µm
Cs = 2.7 mm,
Amp. Contrast = 0.07
B-factor: 80.0 Ų
CTF correction
PSF - delocalization
512 x 512
central pixel
is 1.0
CTF applied:
-0.5 µm
Cs = 2.7 mm,
Amp. Contrast = 0.07
B-factor: 80.0 Ų
Wiener
constant
(related to SSNR)
CTF correction
10 2 0
8 4 3
3 10 18
=
10 0 0
0 0 0
0 0 0
+
0 2 0
0 0 0
0 0 0
+
0 0 0
0 0 0
0 0 0
+
0 0 0
8 0 0
0 0 0
+
0 0 0
0 4 0
0 0 0
+
0 0 0
0 0 3
0 0 0
+
0 0 0
0 0 0
3 0 0
+
0 0 0
0 0 0
0 10 0
+
0 0 0
0 0 0
0 0 18
CTF application
can be
decomposed
into linear
combination

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CTF, PSF and signal delocalization

  • 1. CTF correction PSF - delocalization 512 x 512 central pixel is 1.0
  • 2. CTF correction PSF - delocalization CTF applied: -0.5 µm Cs = 2.7 mm, Amp. Contrast = 0.07 B-factor: 80.0 Ų 512 x 512 central pixel is 1.0
  • 3. CTF correction PSF - delocalization 512 x 512 central pixel is 1.0 CTF applied: -0.5 µm Cs = 2.7 mm, Amp. Contrast = 0.07 B-factor: 80.0 Ų
  • 4. CTF correction PSF - delocalization 512 x 512 central pixel is 1.0 CTF applied: -0.5 µm Cs = 2.7 mm, Amp. Contrast = 0.07 B-factor: 80.0 Ų
  • 5. CTF correction PSF - delocalization 512 x 512 central pixel is 1.0 CTF applied: -0.5 µm Cs = 2.7 mm, Amp. Contrast = 0.07 B-factor: 80.0 Ų Wiener constant (related to SSNR)
  • 6. CTF correction 10 2 0 8 4 3 3 10 18 = 10 0 0 0 0 0 0 0 0 + 0 2 0 0 0 0 0 0 0 + 0 0 0 0 0 0 0 0 0 + 0 0 0 8 0 0 0 0 0 + 0 0 0 0 4 0 0 0 0 + 0 0 0 0 0 3 0 0 0 + 0 0 0 0 0 0 3 0 0 + 0 0 0 0 0 0 0 10 0 + 0 0 0 0 0 0 0 0 18 CTF application can be decomposed into linear combination