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Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Allium sativum L. and
Solanum incanum L. in controlling aphids (Brevicoryne brassicae L.) in rape (Brassica napus L.)
Keywords:
Brevicoryne brassicae, botanical pesticides, efficacy, Tephrosia vogelii,
Allium sativum, Solanum incanum, crude aqueous extracts.
ABSTRACT:
Cabbage aphid (Brevicoryne brassicae L.) is one of the most problematic
pests in smallholder vegetable production, causing significant yield losses in heavy
infestations. Current control strategy focuses on use of synthetic pesticides that
consequently lead to decimation of natural enemies, development of insect
resistance and resurgence and upset biodiversity. Botanical pesticides have been used
widely in smallholder farmers but not much documented literature exists on efficacy
of these products. A field trial was done to assess the efficacy of crude aqueous
extracts of Tephrosia vogelii, Allium sativum and Solanum incanum in controlling
Brevicoryne brassicae in Brassica napus production. The trial was laid in a randomized
complete block design (RCBD) with five treatments replicated four times. The five
treatments used in the experiment were T. vogelii, A. sativum, S. incanum,
dimethoate and control. Wingless adult female aphids were inoculated three weeks
after transplanting of seedlings. Spraying and data collection were done weekly for
four weeks. Data was collected on aphid nymph and adult counts on the third leaf
from the aerial plant part of randomly selected plants from each treatment for
24 hours after the application of treatments and total plant fresh weight per each
treatment. There were significant differences (p<0.05) in the efficacy of botanicals
and synthetics in controlling aphids, with dimethoate being the most effective
followed by T. vogelii, A. sativum, S. incanum and lastly the control. The treatments
applied had a significant effect (p<0.05) on the yield of rape. It was concluded that
T. vogelii, S. incanum and A. sativum aqueous crude extracts have some pesticidal
effects on aphid in rape production.
157-163 | JRA | 2013 | Vol 2 | No 1
This article is governed by the Creative Commons Attribution License (http://creativecommons.org/
licenses/by/2.0), which gives permission for unrestricted use, non-commercial, distribution and
reproduction in all medium, provided the original work is properly cited.
www.jagri.info
Journal of Research in
Agriculture
An International Scientific
Research Journal
Authors:
Shepherd Mudzingwa1
,
Simbarashe Muzemu*,1
and James Chitamba2
-
Institution:
1. Department of
Horticulture, Faculty of
Natural Resources
Management and
Agriculture, Midlands State
University, P. Bag 9055,
Gweru, Zimbabwe.
2. Department of Agronomy,
Faculty of Natural Resources
Management and
Agriculture, Midlands State
University, P. Bag 9055,
Gweru, Zimbabwe.
Corresponding author:
Simbarashe Muzemu.
Email:
smuzemu@gmail.com,
muzemus@msu.ac.zw
Web Address:
http://www.jagri.info
documents/AG0040.pdf. Dates:
Received: 09 Mar 2013 Accepted: 03 May 2013 Published: 16 May 2013
Article Citation:
Shepherd Mudzingwa, Simbarashe Muzemu and James Chitamba.
Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Allium sativum L.
and Solanum incanum L. in controlling aphids (Brevicoryne brassicae L.) in rape
(Brassica napus L.)
Journal of Research in Agriculture (2013) 2(1): 157-163
Original Research
Journal of Research in Agriculture
JournalofResearchinAgriculture An International Scientific Research Journal
INTRODUCTION
Rape, Brassica napus L., is one of the most
important and widely grown vegetable crops for resource
poor small scale farmers of Zimbabwe for subsistence
and a source of income. Pests and diseases are major
constraints causing losses in quality, marketability and
up to 80% on yield (Dobson et al., 2002). Due to the
resource constraints such as pesticides, the management
and control of pests and diseases is poor in most small
scale farming sector. Rape is mostly prone to aphid
attack in most parts of the world where it is produced
mainly because of resource constraints (Langer, 1999).
According to Dobson et al. (2002), aphids represent
pests that are not effectively controlled by the current
pest management measures in small holder farming.
Botanical extracts are naturally occurring
insecticide compounds derived from plants. They contain
groups of active ingredients of diverse chemical nature
and have an average residual life of 2-5 days thus
reducing pressure for selection of resistant types relative
to synthetics (Johnson, 1998). Wilson (2001) also
reported that botanical extracts are known to have a
knock down effect on insects at concentrations harmless
to mammals. The botanicals can be processed into
various forms and these include crude plant material,
plant extracts and pure chemicals isolated from the plants
(Johnson, 1998). Application of these natural insecticides
by farmers and natives revealed a lot of practices based
on superstition have to be demystified with scientific
findings (Aguayo, 2000). Despite the growth in
widespread use of botanicals in smallholder farmers of
Zimbabwe, not much scientific research has been done in
terms of determining efficacy of the specifically used
botanical products, their toxicity, effective application
rates and selection of the most effective types (Roy et al.,
2005).
Scientific research on the use of botanical
pesticides is now gaining momentum since the consumer
preference has also shifted from foods that are grown
with chemicals to the organically grown foods.
(Nas, 2004). The agrochemical industry is also facing
problems such as pest resistance to pesticides and the
high costs of developing new products, thus there has
been a renewed interest in biological control agents and
naturally occurring pesticides to help curb the problem of
pests (Bridge, 1996). It is therefore apparent that a cheap
and environmentally alternative, like botanical
pesticides, would be an appropriate solution. The
research was conducted also in response to the
continuing need to combat insect pests which have not
yielded to effective control, thus the need to find
alternatives for existing chemicals and to provide a ready
supply in future to meet insecticide shortages. Thus
natural methods of plant protection, which use locally
available resources, have assumed a new importance in
an age when a host of commercial products are available.
The present study was carried out with the main
objective of assessing the efficacy of crude aqueous
extracts of Tephrosia vogelii, Allium sativum and
Solanum incanum in controlling Brevicoryne brassicae
in Brassica napus production.
MATERIALS AND METHODS
Study site
The research was done at Fambidzanai
Permaculture Centre, located at 7°51'50 S and 31°1'47 E,
in Harare, Zimbabwe. The site is in natural farming
region IIa of Zimbabwe's Agroecological Zones and
receives an average annual rainfall of 750-1000 mm. It is
on an altitude of 1150 m above sea level and has sandy
clay loam soils.
Land preparation and seedling establishment
Planting stations were dug using hoes and 20
beds measuring 2 m2
each were prepared. Beds and
transplants were spaced at 0.5 m and 0.3 m × 0.3 m
respectively. Seedlings were raised in a greenhouse
nursery. A basal dressing of vermiculite compost manure
was applied a week before transplanting at a rate of 10
158 Journal of Research in Agriculture (2013) 2(1): 157-163
Mudzingwa, 2013
kg per 2 m2
as a nutrient supplement to facilitate good
crop stand establishment. Liquid manure prepared from
piggery manure and diluted at a ratio of 1:4, was applied
three weeks after transplanting as top dress at a rate of
250 ml per planting station and at two weeks interval
thereafter. Irrigation and weed management followed
general recommended crop production practices.
Test pest inoculation, spraying and sampling
Ten adult wingless female aphids were
inoculated three weeks after transplanting of seedlings.
Ten aphids were introduced to each plant and left for 14
days to allow them to acclimatize and reproduce before
spraying. From the 5th
week after transplanting, spraying
began and it was done at weekly intervals for four weeks.
A hand held sprayer fitted with a cone nozzle was used
for spraying with prepared concentrates without further
dilution. Dimethoate was used as per manufacturer label
instructions. Plain water was applied to simulate water
diluents in other sprayers. Six sample plants were
randomly selected from each seedbed and aphid count
was done on the third leaf from the aerial plant part.
Enumeration of aphids in situ was done using handheld
lenses for 24 hours after treatments. Sampling was done
weekly for four consecutive weeks.
Experimental design and treatments
A randomized complete block design (RCBD),
with five treatments replicated for four times (four
blocks) was used (Table 1).
Preparation of crude aqueous plant extracts
Bulbs of A. sativum were washed, peeled and
sterilized using sodium hypochlorite before preparation.
Cloves were pounded using pestle and mortar, measured
into 300 g and soaked in 1 litre of water. The mixtures
were thoroughly stirred with a spatula and left to settle
for 24 hours. Mutton cloth was used to filter residue
before spraying (Stoll, 2000; Berger, 1994). 200 g and
500 g fresh leaves of T. vogelii and S. incanum
respectively were processed using procedures same as
for A. sativum.
Data collection and analysis
Data on aphid population and leaf fresh weight
was collected. Data was analyzed using GenStat
Discovery version 3.0. Least significant difference
(LSD) was used in mean separation at 5% significance
level.
RESULTS AND DISCUSSION
Efficacy of botanical extracts on controlling nymph
and adult aphid population
There was a significant difference (p<0.05) in
nymph aphid population among the treatments in all the
time periods (weeks 1, 2, 3 and 4). From week two up to
week four, dimethoate achieved 100% than control as
evidenced by 0 nymph aphid population hence it was the
most effective control agent available (Figure 1). The
control was significantly different (p<0.05) from all the
other treatments except in week one where it had the
same effect as S. incanum. The A. sativum and T .vogelii
treatments were significantly different from each other in
weeks three and four. Dimethoate significantly differed
from all botanical treatments except in week four where
it had the same effect as T. vogelii (Figure 1). Generally,
dimethoate was the most effective followed by T. vogelii,
A. sativum, S. incanum and lastly the control.
The treatments had significant effect (p<0.05) on
adult aphid population in all the four sampling times
(Figure 2). Mean adult aphid populations were highest
Journal of Research in Agriculture (2013) 2(1): 157-163 159
Mudzingwa, 2013
Table 1: Different treatments sprayed and
their concentrations
Treatment Concentration (mass/
volume of water)
Tephrosia vogelii 200 g/ℓ
Allium sativum 300 g/ℓ
Solanum incanum 500 g/ℓ
Dimethoate 10 ml/ℓ (0.1%) (v/v)
Plain tape water (control) 1 ℓ
(42.38) in the control and lowest (0.5) in dimethoate. The
control treatment was equally less effective as the
Solanum incanum L. in controlling adult aphids during
the first week (Figure 2). Among the botanical extracts,
Tephrosia vogelii L. was the most effective in controlling
adult aphids followed by Allium sativum L. and lastly
S. incanum. T. vogelii and A. sativum performed equally
effective in control adult aphids in all the sampling
periods except at week 1 (Figure 2).
The study demonstrated that aqueous crude
extracts of test plants were effective in controlling aphid
nymph and adult populations. Dimethoate and T. vogelii
were the most effective treatments in the control of
aphids as evidenced by lowest nymph and adult
populations accompanied by highest fresh rape leaf
weight as compared to other treatments. There was an
increase in adult and nymph aphid infestation coupled
with a decrease in plant fresh weight in the control
treatment. These differences in efficacies of the
treatments might be due to the differences in the
chemical, physical properties and potency of active
compounds in the various treatments used. The findings
that T. vogelii and A. sativum reduces aphid population
agree with the results obtained by Braizer (2001) and
those of Buss and Park-Brown (2002) on S. incanum
effectiveness.
Reduced efficacy in botanical pesticides relative
to synthetic pesticide may be attributed to the fact that
spray droplets of botanicals did not come into direct
contact with the aphids due to reduced mobility of
nymph aphids such that those in leaf folds and on
underside were spared of spray droplets. Dimethoate, in
contrary, is a systemic pesticide which once taken into
the plant system will result in toxicity to sucking pests
when they suck sap (Dobson et al., 2002).
Botanical products were effective in reducing
aphid populations due to the fact that they contain a
group of active ingredients which act concertedly on
both behavioral and physiological processes and hence
their effectiveness (Sexana, 1987). T. vogelii contains a
compound named rotenone which is responsible for the
efficacy of the extract. Rotenone (C23H2206) is a
selective, non specific and non systemic chemical which
is both a contact and stomach poison to insects. It kills
pests slowly by stopping them from feeding almost
immediately and exerts its toxic action by acting as a
160 Journal of Research in Agriculture (2013) 2(1): 157-163
Mudzingwa, 2013
Figure 1: Effect of different crude botanical extracts
on mean nymph aphid population
Tephrosia vogelii ControlSolanum incanumAllium sativumDimethoate
Figure 2: Effect of different crude botanical extracts
on mean adult aphid population
Tephrosia vogeliiDimethoate Allium sativum Solanum incanum Control
Figure 3: Effect of different crude botanical extracts
on mean rape yield
T. vogeliiDimethoate A. sativum S. incanum Control
general inhibitor of cellular respiration to a wide range of
insect pests including aphids (Gaskins et al., 1992).
T. vogelii has multiple action properties of rotenone.
Rotenone exhibits teratogenic effect and reproductive
effects if applied to pests. Teratogenicity is when the
pests give birth to deformed off-springs as a result of an
applied selection pressure, thus resulting in reduced
nymph population on T. vogelii treatment (Briggs, 1992).
The effectiveness of A. sativum can be attributed
to the fact that it contains a group of closely related
compounds (allicins) produced upon crushing of the
cloves and are responsible for the pesticidal properties of
Allium spp. Research findings have shown that allicin in
Alliums can be classified as insecticides, acaricides,
nematicides, herbicides, fungicides, bactericides and
repellents against arthropods (Tada et al., 1988). On the
other hand, solanine (C45H73NO15) in S. incanum is a
poisonous glyco-alkaloid, made of the alkaloid
solanidine and carbohydrate (glycol-) side-chains with
both fungicidal and pesticidal properties. It interacts with
the growth and development of the insect and the
interaction may consequently lead to death. These
characteristic differences in properties account for the
variations in efficacy of the tested plant extracts.
The results from the present study agree with
those of Elwell and Maas (1995), where they observed
that T. vogelii, when used with warm water provides the
best protection against aphids. A. sativum was second
and black nightshade was third in terms of aphid
populations. According to Aguayo (2000), most
botanicals used for plant protection exhibit an insect
deterrent rather than insecticidal effect. Therefore
reduction in level of efficacy within the botanicals may
be due to the exhibition of deterrent effects as such from
solanine in S. incanum. This coupled with their slow
activity, rapid degradation by UV light, short life cycle
for most aphids (five to six days) and the congregation
on underside of leaves and on leaf folds of aphids, may
have contributed to the less effectiveness of botanicals in
controlling aphids relative to the synthetic dimethoate
(Davis, 2006).
The control had highest aphid populations
throughout the sampling period. This concurs with the
findings of Minja et al. (2001) who noted that if aphid
population is left unchecked, they enormously multiply
and are only limited with food source besides other
selection pressures. The rapid proliferation can be
attributed to their rapid development time (8-12 days)
from first instar nymph to adult, reproduction is possible
in absence of males and extended reproductive life-span
(30 days at 5-6 nymphs/day).
Effect of aphid infestation on rape fresh yield
The treatments applied had a significant effect
(p<0.05) on the yield of rape. Highest mean rape fresh
yield was recorded on dimethoate treatment followed by
T. vogelii, A. sativum and S. incanum. The lowest yield
was recorded from the control treatment. Dimethoate and
T. vogelii had statistically similar yields and so were
S. incanum and A. sativum (Figure 3). However, the
control treatment had yield which was significantly
different from all the treatments (Figure 3).
There was an inverse variation relationship
between aphid populations per unit area and weight gain
of a plant. Both aphid nymphs and the adults are sap
sucking pests that feed by extracting photosynthates
which are the sources of energy and vital raw materials
for cell division and elongation for the growth and
development in the plant. The leaf becomes severely
distorted when the saliva of aphids are injected into it
(Alford, 1999). Hamman (1985) observed that if left
unchecked, aphids in agro ecology can stunt plant
growth, deform and discolor leaves and fruits or cause
galls on leaves, stems and roots. According to
Minja et al., (2001) aphid infestation and feeding
damage results in curling and yellowing of leaves and
stunting plant growth. This reduces the leaf area index
and consequently the total quantities of carbohydrates
that contribute to plant biomass resulting in lower fresh
Journal of Research in Agriculture (2013) 2(1): 157-163 161
Mudzingwa, 2013
yields.
CONCLUSION
T. vogelii, S. incanum and A. sativum aqueous
crude extracts have some pesticidal effects on rape aphid
B. brassicae in B. napus production. The most
efficacious crude botanical extract was T. vogelii in
controlling both adult and nymph aphid populations as
the recommended synthetic pesticide, dimethoate. The
three extracts managed to effectively reduce the losses in
yield caused by aphids, giving higher fresh weight
compared to the control.
ACKNOWLEDGEMENTS
The authors are grateful to Fambidzanai
Permaculture for funding and hosting the research. The
management and staff of the organization are also
acknowledged for providing technical assistance and
support.
REFERENCES
Aguayo GS. 2000. IPM World Textbook, Botanical
Insecticides.http://ipmworld.umn.edu./chapters/silver-
Aguayo.htm. Accessed 28 March 2010
Alford DV. 1999. A Textbook of Agricultural
Entomology. Blackwell Science Ltd, Cambridge, UK.
Berger A. 1994. Using natural pesticides: Current and
future perspectives. A report for the Plant Protection
Improvement in Botswana, Zambia and Tanzania.
Bridge J. 1996. Nematode management in sustainable
and subsistence agriculture. Annual Reviews of
Phytopathology, 34:201–25.
Briggs SA. 1992. Basic guide to pesticides: Their
characteristics and hazards. Hemisphere Publishing
Corporation. Philadelphia, PA.
Buss EA and Park-Brown SG. 2002. Natural Products
for Insect Management. Chapman Hall, London.
Davis UC. 2006. Pests in Landscapes and Gardens. IPM
Education and Publications. University of California.
Dobson H, Cooper J, Manyangirirwa W, Karuma J
and Chiimba W. 2002. Integrated vegetable pest
management: safe and sustainable protection of small-
scale brassicas abd tomatoes, Natural Resource Institute,
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Elwell H and Maas A. 1995. Natural pest and disease
control, Natural Farming Network. Mambo Press,
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Gaskins MH, White GA and Martin FW. 1992.
Tephrosia vogelii; A source of rotenoids for insecticidal
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Hamman PJ. 1985. Aphids on trees and shrubs. L-1227,
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plants as botanical pesticides against aphid species.
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Tada M, Hiroe Y, Kiyohara S and Suzuki S. 1988.
Nematicidal and antimicrobial constituents from
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Journal of Research in Agriculture (2013) 2(1): 157-163 163
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Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Allium sativum L. and Solanum incanum L. in controlling aphids (Brevicoryne brassicae L.) in rape (Brassica napus L.)

  • 1. Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Allium sativum L. and Solanum incanum L. in controlling aphids (Brevicoryne brassicae L.) in rape (Brassica napus L.) Keywords: Brevicoryne brassicae, botanical pesticides, efficacy, Tephrosia vogelii, Allium sativum, Solanum incanum, crude aqueous extracts. ABSTRACT: Cabbage aphid (Brevicoryne brassicae L.) is one of the most problematic pests in smallholder vegetable production, causing significant yield losses in heavy infestations. Current control strategy focuses on use of synthetic pesticides that consequently lead to decimation of natural enemies, development of insect resistance and resurgence and upset biodiversity. Botanical pesticides have been used widely in smallholder farmers but not much documented literature exists on efficacy of these products. A field trial was done to assess the efficacy of crude aqueous extracts of Tephrosia vogelii, Allium sativum and Solanum incanum in controlling Brevicoryne brassicae in Brassica napus production. The trial was laid in a randomized complete block design (RCBD) with five treatments replicated four times. The five treatments used in the experiment were T. vogelii, A. sativum, S. incanum, dimethoate and control. Wingless adult female aphids were inoculated three weeks after transplanting of seedlings. Spraying and data collection were done weekly for four weeks. Data was collected on aphid nymph and adult counts on the third leaf from the aerial plant part of randomly selected plants from each treatment for 24 hours after the application of treatments and total plant fresh weight per each treatment. There were significant differences (p<0.05) in the efficacy of botanicals and synthetics in controlling aphids, with dimethoate being the most effective followed by T. vogelii, A. sativum, S. incanum and lastly the control. The treatments applied had a significant effect (p<0.05) on the yield of rape. It was concluded that T. vogelii, S. incanum and A. sativum aqueous crude extracts have some pesticidal effects on aphid in rape production. 157-163 | JRA | 2013 | Vol 2 | No 1 This article is governed by the Creative Commons Attribution License (http://creativecommons.org/ licenses/by/2.0), which gives permission for unrestricted use, non-commercial, distribution and reproduction in all medium, provided the original work is properly cited. www.jagri.info Journal of Research in Agriculture An International Scientific Research Journal Authors: Shepherd Mudzingwa1 , Simbarashe Muzemu*,1 and James Chitamba2 - Institution: 1. Department of Horticulture, Faculty of Natural Resources Management and Agriculture, Midlands State University, P. Bag 9055, Gweru, Zimbabwe. 2. Department of Agronomy, Faculty of Natural Resources Management and Agriculture, Midlands State University, P. Bag 9055, Gweru, Zimbabwe. Corresponding author: Simbarashe Muzemu. Email: smuzemu@gmail.com, muzemus@msu.ac.zw Web Address: http://www.jagri.info documents/AG0040.pdf. Dates: Received: 09 Mar 2013 Accepted: 03 May 2013 Published: 16 May 2013 Article Citation: Shepherd Mudzingwa, Simbarashe Muzemu and James Chitamba. Pesticidal efficacy of crude aqueous extracts of Tephrosia vogelii L., Allium sativum L. and Solanum incanum L. in controlling aphids (Brevicoryne brassicae L.) in rape (Brassica napus L.) Journal of Research in Agriculture (2013) 2(1): 157-163 Original Research Journal of Research in Agriculture JournalofResearchinAgriculture An International Scientific Research Journal
  • 2. INTRODUCTION Rape, Brassica napus L., is one of the most important and widely grown vegetable crops for resource poor small scale farmers of Zimbabwe for subsistence and a source of income. Pests and diseases are major constraints causing losses in quality, marketability and up to 80% on yield (Dobson et al., 2002). Due to the resource constraints such as pesticides, the management and control of pests and diseases is poor in most small scale farming sector. Rape is mostly prone to aphid attack in most parts of the world where it is produced mainly because of resource constraints (Langer, 1999). According to Dobson et al. (2002), aphids represent pests that are not effectively controlled by the current pest management measures in small holder farming. Botanical extracts are naturally occurring insecticide compounds derived from plants. They contain groups of active ingredients of diverse chemical nature and have an average residual life of 2-5 days thus reducing pressure for selection of resistant types relative to synthetics (Johnson, 1998). Wilson (2001) also reported that botanical extracts are known to have a knock down effect on insects at concentrations harmless to mammals. The botanicals can be processed into various forms and these include crude plant material, plant extracts and pure chemicals isolated from the plants (Johnson, 1998). Application of these natural insecticides by farmers and natives revealed a lot of practices based on superstition have to be demystified with scientific findings (Aguayo, 2000). Despite the growth in widespread use of botanicals in smallholder farmers of Zimbabwe, not much scientific research has been done in terms of determining efficacy of the specifically used botanical products, their toxicity, effective application rates and selection of the most effective types (Roy et al., 2005). Scientific research on the use of botanical pesticides is now gaining momentum since the consumer preference has also shifted from foods that are grown with chemicals to the organically grown foods. (Nas, 2004). The agrochemical industry is also facing problems such as pest resistance to pesticides and the high costs of developing new products, thus there has been a renewed interest in biological control agents and naturally occurring pesticides to help curb the problem of pests (Bridge, 1996). It is therefore apparent that a cheap and environmentally alternative, like botanical pesticides, would be an appropriate solution. The research was conducted also in response to the continuing need to combat insect pests which have not yielded to effective control, thus the need to find alternatives for existing chemicals and to provide a ready supply in future to meet insecticide shortages. Thus natural methods of plant protection, which use locally available resources, have assumed a new importance in an age when a host of commercial products are available. The present study was carried out with the main objective of assessing the efficacy of crude aqueous extracts of Tephrosia vogelii, Allium sativum and Solanum incanum in controlling Brevicoryne brassicae in Brassica napus production. MATERIALS AND METHODS Study site The research was done at Fambidzanai Permaculture Centre, located at 7°51'50 S and 31°1'47 E, in Harare, Zimbabwe. The site is in natural farming region IIa of Zimbabwe's Agroecological Zones and receives an average annual rainfall of 750-1000 mm. It is on an altitude of 1150 m above sea level and has sandy clay loam soils. Land preparation and seedling establishment Planting stations were dug using hoes and 20 beds measuring 2 m2 each were prepared. Beds and transplants were spaced at 0.5 m and 0.3 m × 0.3 m respectively. Seedlings were raised in a greenhouse nursery. A basal dressing of vermiculite compost manure was applied a week before transplanting at a rate of 10 158 Journal of Research in Agriculture (2013) 2(1): 157-163 Mudzingwa, 2013
  • 3. kg per 2 m2 as a nutrient supplement to facilitate good crop stand establishment. Liquid manure prepared from piggery manure and diluted at a ratio of 1:4, was applied three weeks after transplanting as top dress at a rate of 250 ml per planting station and at two weeks interval thereafter. Irrigation and weed management followed general recommended crop production practices. Test pest inoculation, spraying and sampling Ten adult wingless female aphids were inoculated three weeks after transplanting of seedlings. Ten aphids were introduced to each plant and left for 14 days to allow them to acclimatize and reproduce before spraying. From the 5th week after transplanting, spraying began and it was done at weekly intervals for four weeks. A hand held sprayer fitted with a cone nozzle was used for spraying with prepared concentrates without further dilution. Dimethoate was used as per manufacturer label instructions. Plain water was applied to simulate water diluents in other sprayers. Six sample plants were randomly selected from each seedbed and aphid count was done on the third leaf from the aerial plant part. Enumeration of aphids in situ was done using handheld lenses for 24 hours after treatments. Sampling was done weekly for four consecutive weeks. Experimental design and treatments A randomized complete block design (RCBD), with five treatments replicated for four times (four blocks) was used (Table 1). Preparation of crude aqueous plant extracts Bulbs of A. sativum were washed, peeled and sterilized using sodium hypochlorite before preparation. Cloves were pounded using pestle and mortar, measured into 300 g and soaked in 1 litre of water. The mixtures were thoroughly stirred with a spatula and left to settle for 24 hours. Mutton cloth was used to filter residue before spraying (Stoll, 2000; Berger, 1994). 200 g and 500 g fresh leaves of T. vogelii and S. incanum respectively were processed using procedures same as for A. sativum. Data collection and analysis Data on aphid population and leaf fresh weight was collected. Data was analyzed using GenStat Discovery version 3.0. Least significant difference (LSD) was used in mean separation at 5% significance level. RESULTS AND DISCUSSION Efficacy of botanical extracts on controlling nymph and adult aphid population There was a significant difference (p<0.05) in nymph aphid population among the treatments in all the time periods (weeks 1, 2, 3 and 4). From week two up to week four, dimethoate achieved 100% than control as evidenced by 0 nymph aphid population hence it was the most effective control agent available (Figure 1). The control was significantly different (p<0.05) from all the other treatments except in week one where it had the same effect as S. incanum. The A. sativum and T .vogelii treatments were significantly different from each other in weeks three and four. Dimethoate significantly differed from all botanical treatments except in week four where it had the same effect as T. vogelii (Figure 1). Generally, dimethoate was the most effective followed by T. vogelii, A. sativum, S. incanum and lastly the control. The treatments had significant effect (p<0.05) on adult aphid population in all the four sampling times (Figure 2). Mean adult aphid populations were highest Journal of Research in Agriculture (2013) 2(1): 157-163 159 Mudzingwa, 2013 Table 1: Different treatments sprayed and their concentrations Treatment Concentration (mass/ volume of water) Tephrosia vogelii 200 g/ℓ Allium sativum 300 g/ℓ Solanum incanum 500 g/ℓ Dimethoate 10 ml/ℓ (0.1%) (v/v) Plain tape water (control) 1 ℓ
  • 4. (42.38) in the control and lowest (0.5) in dimethoate. The control treatment was equally less effective as the Solanum incanum L. in controlling adult aphids during the first week (Figure 2). Among the botanical extracts, Tephrosia vogelii L. was the most effective in controlling adult aphids followed by Allium sativum L. and lastly S. incanum. T. vogelii and A. sativum performed equally effective in control adult aphids in all the sampling periods except at week 1 (Figure 2). The study demonstrated that aqueous crude extracts of test plants were effective in controlling aphid nymph and adult populations. Dimethoate and T. vogelii were the most effective treatments in the control of aphids as evidenced by lowest nymph and adult populations accompanied by highest fresh rape leaf weight as compared to other treatments. There was an increase in adult and nymph aphid infestation coupled with a decrease in plant fresh weight in the control treatment. These differences in efficacies of the treatments might be due to the differences in the chemical, physical properties and potency of active compounds in the various treatments used. The findings that T. vogelii and A. sativum reduces aphid population agree with the results obtained by Braizer (2001) and those of Buss and Park-Brown (2002) on S. incanum effectiveness. Reduced efficacy in botanical pesticides relative to synthetic pesticide may be attributed to the fact that spray droplets of botanicals did not come into direct contact with the aphids due to reduced mobility of nymph aphids such that those in leaf folds and on underside were spared of spray droplets. Dimethoate, in contrary, is a systemic pesticide which once taken into the plant system will result in toxicity to sucking pests when they suck sap (Dobson et al., 2002). Botanical products were effective in reducing aphid populations due to the fact that they contain a group of active ingredients which act concertedly on both behavioral and physiological processes and hence their effectiveness (Sexana, 1987). T. vogelii contains a compound named rotenone which is responsible for the efficacy of the extract. Rotenone (C23H2206) is a selective, non specific and non systemic chemical which is both a contact and stomach poison to insects. It kills pests slowly by stopping them from feeding almost immediately and exerts its toxic action by acting as a 160 Journal of Research in Agriculture (2013) 2(1): 157-163 Mudzingwa, 2013 Figure 1: Effect of different crude botanical extracts on mean nymph aphid population Tephrosia vogelii ControlSolanum incanumAllium sativumDimethoate Figure 2: Effect of different crude botanical extracts on mean adult aphid population Tephrosia vogeliiDimethoate Allium sativum Solanum incanum Control Figure 3: Effect of different crude botanical extracts on mean rape yield T. vogeliiDimethoate A. sativum S. incanum Control
  • 5. general inhibitor of cellular respiration to a wide range of insect pests including aphids (Gaskins et al., 1992). T. vogelii has multiple action properties of rotenone. Rotenone exhibits teratogenic effect and reproductive effects if applied to pests. Teratogenicity is when the pests give birth to deformed off-springs as a result of an applied selection pressure, thus resulting in reduced nymph population on T. vogelii treatment (Briggs, 1992). The effectiveness of A. sativum can be attributed to the fact that it contains a group of closely related compounds (allicins) produced upon crushing of the cloves and are responsible for the pesticidal properties of Allium spp. Research findings have shown that allicin in Alliums can be classified as insecticides, acaricides, nematicides, herbicides, fungicides, bactericides and repellents against arthropods (Tada et al., 1988). On the other hand, solanine (C45H73NO15) in S. incanum is a poisonous glyco-alkaloid, made of the alkaloid solanidine and carbohydrate (glycol-) side-chains with both fungicidal and pesticidal properties. It interacts with the growth and development of the insect and the interaction may consequently lead to death. These characteristic differences in properties account for the variations in efficacy of the tested plant extracts. The results from the present study agree with those of Elwell and Maas (1995), where they observed that T. vogelii, when used with warm water provides the best protection against aphids. A. sativum was second and black nightshade was third in terms of aphid populations. According to Aguayo (2000), most botanicals used for plant protection exhibit an insect deterrent rather than insecticidal effect. Therefore reduction in level of efficacy within the botanicals may be due to the exhibition of deterrent effects as such from solanine in S. incanum. This coupled with their slow activity, rapid degradation by UV light, short life cycle for most aphids (five to six days) and the congregation on underside of leaves and on leaf folds of aphids, may have contributed to the less effectiveness of botanicals in controlling aphids relative to the synthetic dimethoate (Davis, 2006). The control had highest aphid populations throughout the sampling period. This concurs with the findings of Minja et al. (2001) who noted that if aphid population is left unchecked, they enormously multiply and are only limited with food source besides other selection pressures. The rapid proliferation can be attributed to their rapid development time (8-12 days) from first instar nymph to adult, reproduction is possible in absence of males and extended reproductive life-span (30 days at 5-6 nymphs/day). Effect of aphid infestation on rape fresh yield The treatments applied had a significant effect (p<0.05) on the yield of rape. Highest mean rape fresh yield was recorded on dimethoate treatment followed by T. vogelii, A. sativum and S. incanum. The lowest yield was recorded from the control treatment. Dimethoate and T. vogelii had statistically similar yields and so were S. incanum and A. sativum (Figure 3). However, the control treatment had yield which was significantly different from all the treatments (Figure 3). There was an inverse variation relationship between aphid populations per unit area and weight gain of a plant. Both aphid nymphs and the adults are sap sucking pests that feed by extracting photosynthates which are the sources of energy and vital raw materials for cell division and elongation for the growth and development in the plant. The leaf becomes severely distorted when the saliva of aphids are injected into it (Alford, 1999). Hamman (1985) observed that if left unchecked, aphids in agro ecology can stunt plant growth, deform and discolor leaves and fruits or cause galls on leaves, stems and roots. According to Minja et al., (2001) aphid infestation and feeding damage results in curling and yellowing of leaves and stunting plant growth. This reduces the leaf area index and consequently the total quantities of carbohydrates that contribute to plant biomass resulting in lower fresh Journal of Research in Agriculture (2013) 2(1): 157-163 161 Mudzingwa, 2013
  • 6. yields. CONCLUSION T. vogelii, S. incanum and A. sativum aqueous crude extracts have some pesticidal effects on rape aphid B. brassicae in B. napus production. The most efficacious crude botanical extract was T. vogelii in controlling both adult and nymph aphid populations as the recommended synthetic pesticide, dimethoate. The three extracts managed to effectively reduce the losses in yield caused by aphids, giving higher fresh weight compared to the control. ACKNOWLEDGEMENTS The authors are grateful to Fambidzanai Permaculture for funding and hosting the research. The management and staff of the organization are also acknowledged for providing technical assistance and support. REFERENCES Aguayo GS. 2000. IPM World Textbook, Botanical Insecticides.http://ipmworld.umn.edu./chapters/silver- Aguayo.htm. Accessed 28 March 2010 Alford DV. 1999. A Textbook of Agricultural Entomology. Blackwell Science Ltd, Cambridge, UK. Berger A. 1994. Using natural pesticides: Current and future perspectives. A report for the Plant Protection Improvement in Botswana, Zambia and Tanzania. Bridge J. 1996. Nematode management in sustainable and subsistence agriculture. Annual Reviews of Phytopathology, 34:201–25. Briggs SA. 1992. Basic guide to pesticides: Their characteristics and hazards. Hemisphere Publishing Corporation. Philadelphia, PA. Buss EA and Park-Brown SG. 2002. Natural Products for Insect Management. Chapman Hall, London. Davis UC. 2006. Pests in Landscapes and Gardens. IPM Education and Publications. University of California. Dobson H, Cooper J, Manyangirirwa W, Karuma J and Chiimba W. 2002. Integrated vegetable pest management: safe and sustainable protection of small- scale brassicas abd tomatoes, Natural Resource Institute, UK. Elwell H and Maas A. 1995. Natural pest and disease control, Natural Farming Network. Mambo Press, Gweru. Gaskins MH, White GA and Martin FW. 1992. Tephrosia vogelii; A source of rotenoids for insecticidal and pesticidal use. US Dept Agricultural Technology; Bull 1445. Hamman PJ. 1985. Aphids on trees and shrubs. L-1227, Texas Agriculture Extension Service House and Landscape Pest, College Station, Texas. 2pp Johnson DJ. 1998. Vendors of Microbial and Botanical insecticides and Insect M o n i t or i n g D e v i c e s . University of Kentucky. http://uky.edu/Ag/Entomology/ entfacts/ldcrops/ef124.htm. Accessed 04 March 2010. Minja EM, Silim SN and Kimani PM. 2001. Yield losses due to field pests and integrated pest management strategies in brassicas. International Crops Research Institute for the Semi-Arid Tropics, Nairobi, Kenya. Nas MN. 2004. In vitro studies on some natural beverages as botanical pesticides against Erwinia amylovora and Curobacterium flaccumfaciensis subsp. poinsettiae. Turk. J. Agric, 28: 57–61. Roy B, Amin R, Uddin MN, Islam ATMS, Islam MJ and Halder BC. 2005. Leaf extracts of selected wild plants as botanical pesticides against aphid species. Journal of Biological Sciences, 5(2): 201–204. 162 Journal of Research in Agriculture (2013) 2(1): 157-163 Mudzingwa, 2013
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