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Water in hplc 2007


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Water in hplc 2007

  1. 1. Water in HPLC<br />Under the Supervision of: PROF.DR. Sawsan Elmasry<br />Presented by: Reem Tawfeek<br />
  2. 2. Points to be discussed<br />Water used in HPLC.<br />Lab water grades.<br />Specifications of HPLC-grade water.<br /> how to determine water purity?<br />Problems caused by poor quality water.<br />Package and storage.<br />Conclusion.<br />
  3. 3. <ul><li> Water used in HPLC</li></ul>Water for HPLC may be prepared IN HOUSE (analytical labs may prepare its own HPLC water)<br />(N.B. that what most frequently happens in Egyptian labs)<br />Freshly prepared double distilled<br /> water (within 24 hours) will be<br /> convenient for HPLC applications<br />
  4. 4. <ul><li> Water used in HPLC</li></ul>De-ionized water will be INCONVENIENT for HPLC application due to uncertainty of the absence of the microbial contamination<br />OR labs could obtain water from HPLC-grade chemicals supplie<br />
  5. 5. Lab Water Grades<br />
  6. 6. the different water specifications based on the different water types<br />
  7. 7. HPLC-water specifications<br />According to B.P. 2010 water for chromatography is<br />deionized water with a resistivity of not less than 18 MΩ-cm<br />But is this enough<br />It’s possible to have 18 MΩ-cm soup! <br /> Can we use soup for HPLC?????<br />
  8. 8. How to measure water purity?<br /> contaminants in water either<br />
  9. 9. Sources of organic contaminants [Total organic carbon(TOC)]<br />Leaching from purification media, tubing and containers,<br />bacterial contamination<br />absorption from the atmosphere<br />
  10. 10. Poor quality water reduces chromatographic performance by<br />affecting resolution and integration.<br />introducing ghost peaks.<br />altering stationary phase selectivity.<br />and impacting baselines.<br />
  11. 11. Ghost peaks<br />
  12. 12. Isocratic baseline monitoring at 210 nm using 100% high TOC reagent water(10 Mᾩ·cm resistivity, 100 ppb TOC) (1 mL/min).<br />
  13. 13. Using high-purity water(3 ppb TOC)<br />
  14. 14. According to American Society for Testing and Materials ASTM <br />Reagent grade water should contains<br />Total organic carbon (TOC), max , 50 ppb<br />Resistivity not less than 18MΩ-cm<br />
  15. 15. Packing and storage<br />packaged in amber glass bottles <br /> sealed under a nitrogen with Teflon-lined fluorocarbon caps .<br />Unless other instructions stated by SOP(standard operating procedures) Once the bottle is opened it should be used within 24 hours.<br />
  16. 16. Conclusion <br />We should switch from simple resistivity measurement to resistivity and TOC monitoring in order to be able to take reagent water quality for granted. <br />
  17. 17. references<br />W.M.A. Niessen, J. Chromatogr., A 856(1,2),179–197 (1999).<br />W. Byrne, Reverse Osmosis — A Practical Guide for Industrial Users. 2nd ed. (Tall Oaks Publishing,Littleton, Colorado, 2002).<br />Standard Specification for Reagent Water American Society for Testing and Materials International 2001 Annual Book of Standards – Volume 11.01<br />B. Srikanth, Ultrapure Water 15(3), 40–46(1998).<br />K. Clark, M. Retzik, and D. Darbouret, Ultrapure Water 14(2), 21–24, (1997).<br />C. Regnault, I. Kano, D. Darbouret, and S.Mabic,J. Chromatogr., A 1030(1–2), 289–295(2004).<br />B. Stewart and B. Williamson, Am. Biotechnol. Lab., 16–18, December 2001. <br /><br />