Tenascin C expression and localizaMon regulated by Flow and RhoA
Flow and RhoA can regulate the material properMes of
developing valves as measured by Atomic Force Microscopy
No Flow Flow Flow w/ LPA Flow w/ y‐compound
Young's Modulus (Pa)
0 500 1000 1500 2000 2500 3000 No Flow Flow Flow w/ y‐compound Flow w/ LPA
Hong Tan, Stef Biechler, (P<0.05)
and Dr. Dean (Clemson)
• Expression of Fibrous ECM proteins can be
regulated by ﬂow.
• We hypothesize that the mechanical forces
created by pulsaVle ﬂow deform the
cytoskeleton and acVvate the RhoA pathway.
• Experimental manipulaVon of the RhoA
pathway does regulate Fibrous ECM expression
and localizaVon in developing valves
So, if you want to make stronger valves faster, use Flow and RhoA
JD Pods and MJ Yost ‐ USC SOM
Bob Price ‐ USC IRF
HJ Evans‐Anderson – Alumnus
Lorain Junor MUSC
Andrea Roberts Roger Markwald
Samantha SMnson Chip Norris
USC Engineering Northwestern:
John Weidner, Arash Kheradvar Bob Dedman
NIH Support Delphin Dean
Cobre P20‐RR1634 (RRM)
R21 HL080294 (RLG)
RO1 HL086856 (RLG)
NSF RII Bridge A. Tsiaras In Life Aug. 26th, 2005
• The mechanisms of cardiovascular development can invesMgated using 3D
• Developmental mechanisms are likely to be important during regeneraMon.
• The transiMon from capillary to larger caliber blood vessels has been
diﬃcult to model.
• Numerous others.
• Clinical applicaMons.
• Grateful for my present collaborators