Research title urtica dioica plants


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Research title urtica dioica plants

  1. 1. Research Title: Phytochemical Screening and Evaluation of In-Vitro Antioxidant and In-Vivo Hepatoprotective Activity of the Urtica dioica Plant Extract
  2. 2. Investigators information • Ranjit Pandey • • Contact- 00977-9849464685 • Kathmandu Nepal, CiST college • B.Pham 9/21/2013 2
  3. 3. Background • In vitro comes from the Latin term "in glass" and refers to studies of biological properties that are done in a test tube (i.e. under glass. As opposed to in vivo ("in life") studies which are done inside an organism. • Himalayan range of Nepal has vast plant diversity from south 59m to north 8848m which contain wide important to supplement modern allopathic medicine as well as traditional believe of ethno medicinal therapy. • Majority of Nepalese population still depends upon indigenous use of medicinal plants and play vital role in health and economy. • Phytochemical screening is the qualitative analysis of plant chemical constituents like alkaloids, carbohydrates, phenols, flavonoid, tannins etc. 9/21/2013 3
  4. 4. Cont.…. • Antioxidants may offer resistance against oxidative stress by scavenging free radicals, inhibiting lipid peroxidation and by other mechanisms and thereby help in preventing the free radical induced liver diseases. Modern medicine have little to offer alleviation of hepatic diseases and it is chiefly plant based preparation which are employed for their treatment of liver disorder. • Liver diseases are one of the major health problems in the world caused by toxic chemicals, autoimmune disorders, infections and excess consumption of alcohol. • Hepato toxins inhibits anti-oxidation system like superoxide dismutase (SOD), tissue glutathione (GSH) etc. and increases the various liver enzymes (SGPT, SGOT, ALP, serum bilirubin, cholesterol etc.) indicate liver injury. 9/21/2013 4
  5. 5. Rationale of the study • U. dioica has some etnnomedicinal uses including liver tonic. • Literature review suggest very few researches have been conducted on this plant. • More than that research on its clinical use has not been found till date. • That encourages us to conduct the project on this plant. All of these reasons inspire us to conduct the research on this plant. 9/21/2013 5
  6. 6. Statement of the problem • Hepatic disorders are common now a days due to different xenobiotic and chemicals. • No more allopathic medicine are available for liver disorder. • Treatment of infection with liver disorder(e.g. jaundice) is main concern. 9/21/2013 6
  7. 7. Objectives  General:  To perform Phytochemical Screening and Evaluation of In- Vitro Antioxidant and In-Vivo Hepatoprotective activity.  Specific : The specific objectives of the present research project are to :  Collect and identify the proposed plant  Prepare and preserve herbaria and the authentic sample of the collected plants .  Perform the phytochemical screening of sample  Perform the 50% ethanolic extraction of the collected samples  Evaluate antioxidant and hepatoprotactive activities of the extract of collected samples 9/21/2013 7
  8. 8. Materials Appratus • Autoclave, • Hot air oven, • soxhlet apparatus, • Grinder, • Crude drugs, • Beakers, • Whatman filter paper, • entire plant, • rotary flash evaporater chemical • Ethanol • Ascorbic acid • Silymarin • CCl4 • Water • Reagent(Alkaloid,glycoside s,flavonoid,tannin,terpene etc.) 9/21/2013 8
  9. 9. Methodology 1. Collection and identification of crude drugs 2. Extraction of crude materials 3. Phytochemical screening:  Detection of alkaloids:  Detection of carbohydrates  Detection of glycosides  Detection of saponins  Detection of phytosterols  Detection of fixed oils & fats  Detection of resins  Detection of phenols.  Detection of tannins  Detection of flavonoids  Detection of proteins and aminoacids  Detection of diterpenes 4. Tests:  Invitro: antioxident  Invivo: hepatoprotactive in wister albino rats. 9/21/2013 9
  10. 10. Statistical analysis Data will be reported as mean ± SEM. Statistical analysis of data will be carried out by one way ANOVA comparing all test groups versus control using graph-pad software. 9/21/2013 10
  11. 11. Limitations of the study • While phytochemical screening some compound present in very trace amount cannot be detected by simple laboratory reaction and advanced equipmet necessary which cannot be performed in our laboratory. • Discontinuation of electrical supply may interfere with the laboratory function. 9/21/2013 11
  12. 12. Programe schedule Program Schedule Weak 1-5 Weak 6-10 Weak 11-15 Weak 16-20 Weak 21-25 Literature review proposal writing Collection and drying of plant material Extraction and performing the laboratory analysis Result evaluation, validation and interpretation Thesis preparation and defense 9/21/2013 12
  13. 13. Expected outcome 1. Scientific outcomes • To design new drug with potential pharmacological activity. • This will provide a great contribution to the scientific field and provides great help in the further study of these plants. 9/21/2013 13
  14. 14. Expected outcome…. 2. Social outcome In Nepalese society, there is less awareness about these plant species among the people. Many of them do not know about the medicinal uses of these plants. After the completion of the research project, people will be interested in the cultivation, collection and conversion of these species 9/21/2013 14
  15. 15. Expected outcome…. 3. Economic outcomes • In developing countries like Nepal, such studies will help in the development of economic status and such unexplored medicines will be utilized for the improvement of health status of people. 9/21/2013 15
  16. 16. Financial Plan Total expenditure of the project work is estimated as follows: S. No. Particulars Cost (NRs.) 1. Chemicals and reagents 15000.00 2 Solvents 3000.00 3 Albino rats 4000.00 4 Rat diet 3000.00 5 Crude drugs 1500.00 6 Correspondences 2000.00 7 Books and journals 1000.00 8 Training (mouse handling) 5000.00 9 Printing and publishing 2000.00 10 Miscellaneous 2000.00 Total 38500.00 9/21/2013 16
  17. 17. References • 1. Gaire B. P. and S. L.;, Medicinal Plant Diversity and their Pharmacological Aspects of Nepal Himalayas. Pharmacognosy Journal, 2011. 3(25). • 2. Chhetri H. P., et al., PHYTOCHEMICAL AND ANTIMICROBIAL EVALUATIONS OF SOME MEDICINAL PLANTS OF NEPAL. KATHMANDU UNIVERSITY JOURNAL OF SCIENCE, ENGINEERING AND TECHNOLOGY, 2008. 1(5): p. 49-54. • 3. Tiwari P., et al., Phytochemical screening and Extraction: A Review. INTERNATIONALE PHARMACEUTICASCIENCIA, 2011. 1(1). • 4. Qureshi M. N., et al., In-vitro Antioxidant and In-vivo Hepatoprotective activity of Leucas ciliata leaves. Records of Natural Products • 2010. 4(2): p. 124-130. • 5. Shashank T., et al., Evaluation of Hepatoprotective activity of Stem Bark of Homalium zeylanicum in Rats. International Journal of PharmTech Research, 2011. 3(3): p. 1630-1634. • 6. Kumar P., et al., anti-oxident and hepatoprotective activity of tubers of 9/21/2013 17
  18. 18. Thank you 9/21/2013 18