Accurate data are needed on threshold doses for allergenic foods. Data are also needed to demonstrate that available methods of analysis can detect and quantify allergens in foods at or around these threshold levels and that they are robust and fit for purpose. This is of major concern as no curative treatment is currently available for food allergy and accidental ingestion of the culprit food can lead to severe clinical symptoms. Elimination of the problem food ingredient from the diet reduces the risk of allergic reactions but this can lead to other issues such as deficiencies, eating disorders and growth retardation. Emergency medication is available including Antihistamines (H1 blockers), EpiPen (adrenaline-autoinjector) and Corticosteroids. This presentation describes investigations into peanut allergens and their quantitation in highly complex samples.