Normal wbc

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Normal wbc

  1. 1. Morphology of Normal White Blood Cells www.freelivedoctor.com
  2. 2. Granulocytes www.freelivedoctor.com
  3. 3. Neutrophil <ul><li>PMN-Polymorphonuclear </li></ul><ul><li>Leucocytes. </li></ul><ul><li>60-70% WBC </li></ul><ul><li>Appearance: pink granules in cytoplasm, nucleus has 3-4 lobes </li></ul><ul><li>Function: Phagocytosis of bacteria </li></ul><ul><li>Azurophilic (1°) granules are &quot;lysosomes of PMNs&quot;, occur in all leukocytes </li></ul>www.freelivedoctor.com
  4. 4. Eosinophil (Eos) <ul><li>Bilobed nucleus </li></ul><ul><li>2-4% of WBC </li></ul><ul><li>Recruited to sites of inflammation </li></ul><ul><li>Function: Involved in allergy, parasitic infections </li></ul><ul><li>Contains: eosinophilic granules </li></ul><ul><li>Granules contain: major basic protein </li></ul><ul><li>Terminally differentiated </li></ul>Azurophilic granuels www.freelivedoctor.com
  5. 5. Basophil <ul><li>Circulating form of mast cells </li></ul><ul><li>Terminally differentiated </li></ul><ul><li><1% WBC </li></ul><ul><li>Contains: basophilic granules </li></ul><ul><li>Granules contain: histamine and heparin </li></ul><ul><li>IgE receptors </li></ul><ul><li>Involved in allergy </li></ul>www.freelivedoctor.com
  6. 6. Monocyte/ Macrophage <ul><li>Monocyte </li></ul><ul><li>3-8% WBC </li></ul><ul><li>Circulating form (precursor) of tissue macrophages </li></ul><ul><li>Recruited to sites of inflammation </li></ul><ul><li>Macrophages </li></ul><ul><li>Phagocytosis, bacterial killing, antigen presentation </li></ul><ul><li>Peritoneal cavity: peritoneal macrophages </li></ul><ul><li>Lung: alveolar macrophages </li></ul><ul><li>Spleen: splenic macrophages </li></ul><ul><li>Liver: Kupffer cells </li></ul>www.freelivedoctor.com
  7. 7. lymphocyte <ul><li>Appearance: small (same size as RBCs), little visible cytoplasm </li></ul><ul><li>NO specific granules </li></ul><ul><li>20-25% of WBC </li></ul><ul><li>T cells: CMI (for viral infections) </li></ul><ul><li>B cells: humoral (antibody) </li></ul><ul><li>Natural Killer Cells </li></ul>www.freelivedoctor.com
  8. 8. Granulocyte Development <ul><li>Trends: </li></ul><ul><li>Immature  Mature </li></ul><ul><li>Large cell  Small cell </li></ul><ul><li>No granules  Azurophilic (non-specific) granules  Cell-specific granules </li></ul><ul><li>Round nucleus  indented nucleus  U-shaped  multilobed (specific for cell type) </li></ul>www.freelivedoctor.com
  9. 9. www.freelivedoctor.com
  10. 10. myeloblast <ul><ul><li>15-20  m </li></ul></ul><ul><ul><li>large, euchromatic, spherical nucleus (>3 nucleoli) </li></ul></ul><ul><ul><li>basophilic cytoplasm with no granules </li></ul></ul><ul><ul><li>prominent nucleoli </li></ul></ul><ul><ul><li>can be seen in peripheral blood with certain leukemias </li></ul></ul>www.freelivedoctor.com
  11. 11. promyelocyte <ul><li>First recognizable cell in granulopoiesis </li></ul><ul><ul><li>Cannot tell what kind of cell it will become </li></ul></ul><ul><li>17-26 um in diameter </li></ul><ul><li>Largest cell in series </li></ul><ul><li>Large oval nucleus </li></ul><ul><li>Muliple nucleoli </li></ul><ul><li>Golgi Ghost </li></ul><ul><li>Azurophilic (primary) granules in cytoplasm </li></ul><ul><ul><li>Produced only at this stage </li></ul></ul>nucleoli Azurophilic granuels www.freelivedoctor.com
  12. 12. Myelocyte (M1) <ul><li>Spherical nucleus </li></ul><ul><ul><li>Becomes increasingly heterochromatic </li></ul></ul><ul><li>Prominent Golgi apparatus </li></ul><ul><ul><li>Negative image </li></ul></ul><ul><ul><li>Lots of azurophilic granules </li></ul></ul><ul><li>Formation of specific granules </li></ul><ul><ul><li>Emerge from Golgi (cis face) complex </li></ul></ul><ul><ul><li>Characteristic staining reactions for each line </li></ul></ul><ul><li>Last stage that can do mitosis </li></ul>Late Myelocyte/ Early Metamyelocyte Neutraphil myelocyte Eosinophil myelocyte golgi www.freelivedoctor.com
  13. 13. Metamyelocyte (M2) <ul><li>First stage that is clearly divided into separate lines </li></ul><ul><li>Few hundred granules present in the cytoplasm </li></ul><ul><li>Specific granules outnumber the azurophlic granules 4:1 </li></ul><ul><li>Nucleus </li></ul><ul><ul><li>Heterochromatic </li></ul></ul><ul><ul><li>Indentation deepens to form horse-shoe shape </li></ul></ul>Late neutraphilic metamylocyte Neutraphilic metamylocytes Eosinophil metamylocyte www.freelivedoctor.com
  14. 14. Band Cells (M3) <ul><li>Last immature stage in Neutrophilic series </li></ul><ul><li>Sometimes seen in circulation </li></ul><ul><ul><li>Particularly during states of chronic infection </li></ul></ul><ul><li>Nucleus is elongated and of uniform width </li></ul><ul><li>Nucleus constricts </li></ul><ul><ul><li>2-5 lobes are formed </li></ul></ul><ul><ul><li>PMNs </li></ul></ul>www.freelivedoctor.com
  15. 15. Quiz www.freelivedoctor.com

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