Reproductive Biotechnologies for
Endangered Mammalian Species
by :
Chalissa Nuruzzulfa
Nadya Veronica Margarechaesa Anindi...
Introduction
Extinction

Caused by

Habitat
destruction,
over hunting,
competition
solution

Mantain
Biodiversity

aim

AR...
Conservation
Habitat Preservation :
1. In situ conservation
Live population of animals in their adaptive
environments
2. E...
ART (Assisted Reproductive Techniques)
1.
2.
3.
4.

Gamete Cryopreservation
Artificial Insemination
In Vitro Fertilization...
Gamete Cryopreservation
The ability to freeze and thaw with retention
of viability. Frozen tissue can be stored
indefinite...
Process
collecting
gamete

transfer to
vial/straw
(add
cryoprotect
ant)

storage

Cooling until
–800c

cooling in liquid
n...
Artificial Insemination
Artificial insemination is a means of attaining
pregnancy not involving sexual intercourse. It
del...
Process
observing
ovulation

sperm
injection

sperm
collected

sperm washed

ovulation
In Vitro Fertilization
In vitro fertilisation (IVF) is a process by which an
egg is fertilised by sperm outside the body. ...
Process
Stimulating
ovulation

Removing ovum
from ovary

Embryo
Transfer

Insemination

Embryo
Culture
Intracytoplasmic Sperm Injection
Intracytoplasmic sperm injection (ICSI)
involves injecting a single sperm directly into
a...
Genome Resources Banking
Genome resource banking (GRB) is defined as the
storage of gametes and embryos from threatened
po...
Nuclear Transfer
Nuclear transfer is the technique currently
used in the cloning. The parental nucleus were
replaced by th...
Process
Removing ovum
from ovary

Remove the
nucleus

Implantation

Replace with
donor
nucleus
Embryo Transfer
Superior mother produce high quality of
zygot, than implanted it in to another mother.
It allows a high pr...
h

Thank You
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Reproductive Biotechnologies for Endangered Mammalian Species

  1. 1. Reproductive Biotechnologies for Endangered Mammalian Species by : Chalissa Nuruzzulfa Nadya Veronica Margarechaesa Anindita Rahma Dian Quratu A’yunisa Rizha Hardyansyah Dosen Pengampu : Dr. Dra. Sri Rahayu, Mkes.
  2. 2. Introduction Extinction Caused by Habitat destruction, over hunting, competition solution Mantain Biodiversity aim ART (Assisted Reproductive Techniques) and Conservation
  3. 3. Conservation Habitat Preservation : 1. In situ conservation Live population of animals in their adaptive environments 2. Ex situ conservation Outside its natural environment (realocating)
  4. 4. ART (Assisted Reproductive Techniques) 1. 2. 3. 4. Gamete Cryopreservation Artificial Insemination In Vitro Fertilization Transfer Embryo
  5. 5. Gamete Cryopreservation The ability to freeze and thaw with retention of viability. Frozen tissue can be stored indefinitely in liquid nitrogen at -196°C. It stops temporary the motabolic process without kill the cell.
  6. 6. Process collecting gamete transfer to vial/straw (add cryoprotect ant) storage Cooling until –800c cooling in liquid nitrogen storage (-1960c)
  7. 7. Artificial Insemination Artificial insemination is a means of attaining pregnancy not involving sexual intercourse. It deliberates introduction of semen into female’s vagina or oviduct for the purpose of achieving a pregnancy through fertilisation.
  8. 8. Process observing ovulation sperm injection sperm collected sperm washed ovulation
  9. 9. In Vitro Fertilization In vitro fertilisation (IVF) is a process by which an egg is fertilised by sperm outside the body. The term in vitro, from the Latin meaning in glass, is used, because early biological experiments involving cultivation of tissues outside the living organism from which they came, were carried out in glass containers such as beakers, test tubes, or petri dishes. Today, the term in vitro is used to refer to any biological procedure that is performed outside the organism it would normally be occurring.
  10. 10. Process Stimulating ovulation Removing ovum from ovary Embryo Transfer Insemination Embryo Culture
  11. 11. Intracytoplasmic Sperm Injection Intracytoplasmic sperm injection (ICSI) involves injecting a single sperm directly into an egg in order to fertilise it. The fertilised egg (embryo) is then transferred to the woman’s womb. The major development of ICSI means that as long as some sperm can be obtained (even in very low numbers), fertilisation is possible.
  12. 12. Genome Resources Banking Genome resource banking (GRB) is defined as the storage of gametes and embryos from threatened populations with a deliberate intention to use them in a breeding program at some future occasion. Genetic material from rare and endangered species can be collected and preserved indefinitely in liquid nitrogen (-196°C). These genetic samples can provide 'insurance' for the future against catastrophic events and can potentially be a resource allowing reintroduction of genes into a population via artificial insemination or embryo transfer.
  13. 13. Nuclear Transfer Nuclear transfer is the technique currently used in the cloning. The parental nucleus were replaced by the new one from the donor nucleus to produce the duplicate of donor.
  14. 14. Process Removing ovum from ovary Remove the nucleus Implantation Replace with donor nucleus
  15. 15. Embryo Transfer Superior mother produce high quality of zygot, than implanted it in to another mother. It allows a high production number of the children with a high quality.
  16. 16. h Thank You

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