Spermatogenesis fish 07

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fisiologi ikan

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Spermatogenesis fish 07

  1. 1. SPERMATOGENESIS PADA IKAN
  2. 2.  Def : Proses pembentukan sperma (spermatogonia) Meiosis spermatogenesis spermiogenesis dan spermiasi
  3. 3. TESTIS TERDIRI :tubulla longitudinalis (cyste seminiferusmengandung sel-sel spermatogenik yangberdiferensiasi sinkronidikelilingi sel Sertoli / sel Nutrient).
  4. 4. Testicular HistologyScanning Electron Micrograph Seminiferous Tubules Light Micrograph Interstial Tissue
  5. 5. SEL SERTOLI (SEL NUTRIENT) Ciri-ciri : Bentuk pyramidal panjang bertautan Melekat lamina basalis Ujung sel menjorok ke dalam lumen tubulus / lobulus Fungsi : Penyokong, pelindung dan mengatur nutrisi sel-sel spermatogenik Fagositosis (produk berlebih)  resorbsi lisosom sel Sertoli Sekeresi cairan
  6. 6. Sel Leydig (sel Interstitial) Ciri-ciri : Muncul setelah dewasa Bentuk bulat / poligonal Mempunyai inti di tengah Sitoplasma eosinofil Fungsi : Mensekresi hormone testosterone (meiosis dan maturasi). Mengatur perkembangan kelamin sekunder (dimorfisme dan dikromatisme).
  7. 7. SPERMATOGENESIS- Proliferasi- Tumbuh Spermatogenesis- Mature- Trasnformasi = Spermiogenesis Metamorfosa
  8. 8. Cytological differentiation of spermatogenesis
  9. 9. SPERMATOGONIA MITOSIS Spermatosit primer (4n)  spermtosit sekunder (2n)  spermatid (n)  spermatozoa  milt oleh vas deferens  air
  10. 10. Spermatogonium Spermatid : SpermatogenesisSpermatid  Spermatozoa : Spermiogenesis - aparatus golgi  Akrosom - inti  kepala spermatozoa - sentriol  ekor spermatozoa - plasma membran selubung tubuh - mitokondria  berkumpul di ekorSpermatogonium - dekat lamina basalis, besar bervariasi - inti bulat lonjong, + butir-butir kromatin - nukleoli dekat selaput inti - sitoplasma granuler, < jelas - HE = inti bulat, biru (kadang-kadang mitosis)
  11. 11. Spermatosit Primer - Produk akhir spermatogonium B - inti paling besar, sentral - interfase = (+) butir-butir kromatin halus dan rataSpermatosit Sekunder - produk meiosis I - sel tampak bulat ~ spermatogonium - inti bulat, sentral - interfase = Inti seperti Spermatosit primer dengan spermatid muda
  12. 12. Spermatid - produk meiosis II - hampir berbatasan dengan lumen tubulus seminiferus - inti eksentris, lonjong - berkelompok 4 – 8 - daerah golgi dekat inti, berbatas tidak jelas - mitokondria (butir-butir >>) dalam membran sitoplasmaSpermatozoa Tubulus seminiferus  berkala  dewasa kelamin - mamalia = 50 – 60  - kepala leher ekor = - pangkal - tengah - ujung
  13. 13. SpermatogoniumLOBULAR LUMEN Spermatocyte SpermatidSel Sertoli Sperma Sperm Duct LOBULAR TUBULAR
  14. 14. 9. mature spermatozoa 7. 2nd spermatocytes 6. 1st spermatocytes 8. spermatids11. blood vessel 10. Leydig 4. 5. spermatogonium 2 & 3 spermatogonium 1. stem cell Figure 6. Seminiferous tubule, transverse section
  15. 15. FIG. 1. Chinook salmon testes showing stages of germ cell developmentBar =20 µm primary A spermatogonia transitional spermatogonia late B spermatogoniaprimary spermatocytes secondary spermatocytes spermatids (arrow) and spermatozoa (arrowhead)
  16. 16. Spermatogonia
  17. 17. Spermatogonia
  18. 18. Intermediate spermatocytes
  19. 19. Mature spermatazoa
  20. 20. Spermatozoa tails
  21. 21. Spermatozoa heads
  22. 22. SEM of fish sperm
  23. 23. SPERM COMPARISON NOTE THE 5 MICRON SIZE.Remember 1000 microns = 1 mm, 25 mm = 1 inch
  24. 24. SPERM MOTILITYThe sperm moves by rotating its tail in a spiral motion through the water. This induces waves of force backward propelling the sperm forward. If the sperm hits a hard surface, like an egg, the spiral motion will cause the entire sperm to rotate.
  25. 25. SPERM MOTILITYThe sperm moves by rotating its tail in a spiral motion through the water. This induces waves of force backward propelling the sperm forward. If the sperm hits a hard surface, like an egg, the spiral motion will cause the entire sperm to rotate.
  26. 26. Sperma bergerak : Air laut  media bersifat hipertonik Air tawar  media bersifat hipotonik Sperma aktif bergerak dalam air : 30-60’ (hilang setelah 5”). Sperma ikan teleostei : (-) akrosom  mikrofil (fertilisasi 45 – 60’).

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