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Accelerating your innovation!


       Our modular services
           - Highlights -
The company

• Established in 2000 and profitable since then
• Privately owned, no VC
• 14 skilled employees
• Clients: - big pharma
           - biotech
           from all over the world
           (names and references on request)
• Service provider mainly for R&D
  (business model: fee for service)
• Certified quality management system
  (DIN ISO 9001:2008)
• Complete documentation of our processes using
  sophisticated LIMS
                                                  Certified quality




                                                                      Press here for link to
                                                                      webpage
We are offering highly customized services
in these areas

                                             Cell line
                                             Development
                        Molecular Biology




                         Recombinant         Protein
                         Expression          Purification
Our highlights


        •   Cross organism expression platform
        •   FPPM – Fixed price protein manufacturing




                           Recombinant                 Protein
                           Expression                  Purification
A cross organism expression platform at your fingertips

Key benefits:

•   The fastest way to selecting the best expression
    organism

•   Wide spectrum of expression organisms (E. coli, yeast,
    insect, and mammalian cells)

•   Parallel expression plasmid generation for different
    organisms
    (more than 65 different expression vectors available)

•   Parallel protein expression screening in different
    organisms and determination of activity
Select the best expression host and construct for upscaling
in small scale

This saves time and money

                                                              Press here for link to
                                                              webpage
FPPM · Fixed-price protein manufacturing                FPPM quote request
                                                                                   24
                                                                                  hours
Key benefits:                                             Free feasibility
                                                             analysis


•   Pay only when we succeed                                Receiving
                                                          the target DNA

•   No hidden, extra, or variable costs                  Quality control of
                                                          received DNA

•   Free feasibility analysis within 24 hours
                                                          Generation of
                                                        expression plasmid        assdf
                                                                                    20
•   Guaranteed timeline of 20 business days                                          s
                                                                                 business
                                                                                  days
                                                        Protein expression
•   Guaranteed protein amount of 3 mg from E.coli and
    HEK293
                                                         FPLC purification


•   FPPM covers generation of the expression plasmid,
                                                          Quality control
    protein expression, protein purification and QC.
Fast - 100% transparent - no risk
                                                            Shipment




                                                        Press here for link to
                                                        webpage
Our highlights



                         Cell line
                         Development




        •   ExoIN
        •   FreezeFect
ExoIN – stable mammalian cell lines in record time

Key benefits:

•   Homogenous expression three days after transfection

•   Proof of concept in less than a week

•   Homogenous stable cell line within two weeks

•   No need for clones

•   High tenability for bioactivity or production

•   Works for all cell lines and targets

•   Proven technology: more than 200 cell lines generated
    so far
All inclusive stable homogenous expression in 30
business days
                                                            Press here for link to
                                                            webpage
FreezeFect - ready-to-use transfection competent cells

Key benefits:

• Experience E.coli convenience with mammalian cells

• Cryopreserved transfection competent cells ready-to-use any time

• Elimination of variations caused by the cell culture process

• Works with any Nucleofector™ device (Amaxa, Lonza)

• Available as CHO, HEK293 and HeLa

• Other cell lines on request (even primary cells)

• Technology licensed to Lonza: Nucleofection™ PLUS supplement
  to prepare your own transfection competent cells


                                                                     Press here for link to
                                                                     webpage
Need more detailes or further information?


Please contact us!




                                  www.trenzyme.com

                               contact@trenzyme.com

                               Tel: +49(0)7531/122900

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Trenzyme overview

  • 1. Accelerating your innovation! Our modular services - Highlights -
  • 2. The company • Established in 2000 and profitable since then • Privately owned, no VC • 14 skilled employees • Clients: - big pharma - biotech from all over the world (names and references on request) • Service provider mainly for R&D (business model: fee for service) • Certified quality management system (DIN ISO 9001:2008) • Complete documentation of our processes using sophisticated LIMS Certified quality Press here for link to webpage
  • 3. We are offering highly customized services in these areas Cell line Development Molecular Biology Recombinant Protein Expression Purification
  • 4. Our highlights • Cross organism expression platform • FPPM – Fixed price protein manufacturing Recombinant Protein Expression Purification
  • 5. A cross organism expression platform at your fingertips Key benefits: • The fastest way to selecting the best expression organism • Wide spectrum of expression organisms (E. coli, yeast, insect, and mammalian cells) • Parallel expression plasmid generation for different organisms (more than 65 different expression vectors available) • Parallel protein expression screening in different organisms and determination of activity Select the best expression host and construct for upscaling in small scale This saves time and money Press here for link to webpage
  • 6. FPPM · Fixed-price protein manufacturing FPPM quote request 24 hours Key benefits: Free feasibility analysis • Pay only when we succeed Receiving the target DNA • No hidden, extra, or variable costs Quality control of received DNA • Free feasibility analysis within 24 hours Generation of expression plasmid assdf 20 • Guaranteed timeline of 20 business days s business days Protein expression • Guaranteed protein amount of 3 mg from E.coli and HEK293 FPLC purification • FPPM covers generation of the expression plasmid, Quality control protein expression, protein purification and QC. Fast - 100% transparent - no risk Shipment Press here for link to webpage
  • 7. Our highlights Cell line Development • ExoIN • FreezeFect
  • 8. ExoIN – stable mammalian cell lines in record time Key benefits: • Homogenous expression three days after transfection • Proof of concept in less than a week • Homogenous stable cell line within two weeks • No need for clones • High tenability for bioactivity or production • Works for all cell lines and targets • Proven technology: more than 200 cell lines generated so far All inclusive stable homogenous expression in 30 business days Press here for link to webpage
  • 9. FreezeFect - ready-to-use transfection competent cells Key benefits: • Experience E.coli convenience with mammalian cells • Cryopreserved transfection competent cells ready-to-use any time • Elimination of variations caused by the cell culture process • Works with any Nucleofector™ device (Amaxa, Lonza) • Available as CHO, HEK293 and HeLa • Other cell lines on request (even primary cells) • Technology licensed to Lonza: Nucleofection™ PLUS supplement to prepare your own transfection competent cells Press here for link to webpage
  • 10. Need more detailes or further information? Please contact us! www.trenzyme.com contact@trenzyme.com Tel: +49(0)7531/122900