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This study presents dielectric properties of North Indian Ocean seawater. In all, fourteen seawater samples are collected from Arabian Sea, Lakshadweep Sea, Tip of Bay of Bengal Sea, deep Indian Ocean and Equatorial region. The Von Hipple method is used to measure dielectric properties, both real part å' and imaginary å'', at 5 GHz and 30 °C using automated C-Band microwave bench set up. The dielectric constant å' and dielectric loss å'' are calculated using least square fitting technique. The salinity measurement of seawater samples are done on autosalinometer. Making use of salinity values of all samples and for 5 GHz and 30 °C, static dielectric constant and dielectric loss are estimated by Klein-Swift model and Ellison et al. model. Experimental and theoretical results are compared. This study emphasizes latitude and longitudinal variations of salinity and dielectric properties. The laboratory data obtained are significant for microwave remote sensing applications in physical oceanography.