1. Biosafety in Microbiological
and Biomedical Laboratories
3rd Edition
JY Richmond & RW McKinney (eds.)
5th National Symposium on Biosafety:
A Rational Basis for Biocontainment
2. BMBL
Introduction
1941 - Meyer and Eddie
74 lab associated brucellosis
infections in US
1949 - Sulkin and Pike
222 viral infections (21 fatal)
Only 27% related to known accidents
3. BMBL
Introduction
1951,1965, 1976 - Sulkin and Pike
Surveys for lab-associated infections
More than 5,000 labs
Cumulative total of 3,921cases cited
Most commonly reported:
i Hepatitis i Brucellosis
i Tuberculosis i Tularemia
i Typhoid
i Venzuelan Equine Encephalitis
4. BMBL
Introduction
1951,1965, 1976 - Sulkin and Pike (cont.)
Surveys for lab-associated infections
Fewer than 20% associated with known
accidents
Exposure to infectious aerosols
plausible (but unconfirmed) for >80% of
reported cases
5. Principles of Biosafety
Introduction
Biosafety Levels 1-3
Guidelines to describe combinations of:
Laboratory Practices and Techniques
i Standard Practices
i Special Practices
Safety Equipment (Primary Barriers)
Laboratory Facilities (Secondary Barriers)
6. Principles of Biosafety
Introduction
Biosafety Levels 1-3 Provide
Increasing levels of personnel and
environmental protection
Guidelines for working safely in
microbiological and biomedical
laboratories
7. Lab Practices and Techniques
Introduction
Knowledgeable supervisor
Personnel
i Aware of potential hazards
i Proficient in practices/techniques
Biosafety manual specific to lab
8. Safety Equipment
(Primary Barriers) - Introduction
Biosafety cabinets (BSCs) [BSL-2/3]
Personal protective clothing
i Gloves
i Gowns
Pipetting Devices
Safety centrifuge cups and rotors
Eye and face protection
Respiratory protection [BSL-3]
9. Biosafety Level 1
Introduction
Suitable for work involving well-
characterized agents not known to
cause disease in healthy adult
humans and of minimal potential
hazard to laboratory personnel and
the environment.