1.

2.

1.

2.

stem cell 3.

4.

3.
1.

2.

biomaterial scaffolds

scaffolds

3.
4.
5.
rejection
4.

5.

6.
-

Stem cell
-

(A)

B)
C)
(D)

7.
,

,

,
,

8. Obturator
9.

6-

,

,

,
,

,

,

,

,
,

10.

----- -

http://www.learners.in.th/blogs/posts/247077

,
11.
-

biomimetic scaffold

-

cell

-

stem cell
porous

osteogenic cell

matrix

12.

cell, growth factor, scaffold

3
1...
,
19 biomimetic

stem cell

stem cell
cell
cell

cell
cell

biomimetic

biomimetic

stem cell

(microenviroment), signalin...
D

E

final scaffold

seed stem cell 3

cell

1 scaffold

stirred

(and precultured

statically for 1 week to allow cell a...
28. (A

B)

media
culture

bioreactor

TMJ

(A)
media (B)
perfusion C-F)
media

SEM)
(C and D) Flat

flow
E

media

flow

...
34.

degradation

calvarial defect

collagen-calcium phosphate scaffold
4

implant

A.
degration

scaffold

B.

defect
cel...
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สัมมนา

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สัมมนา

  1. 1. 1. 2. 1. 2. stem cell 3. 4. 3. 1. 2. biomaterial scaffolds scaffolds 3. 4. 5. rejection 4. 5. 6. - Stem cell
  2. 2. - (A) B) C) (D) 7. , , , , 8. Obturator 9. 6- , , ,
  3. 3. , , , , , , 10. ----- - http://www.learners.in.th/blogs/posts/247077 ,
  4. 4. 11. - biomimetic scaffold - cell - stem cell porous osteogenic cell matrix 12. cell, growth factor, scaffold 3 13. 14. biomimetic scaffold scaffolds cell scaffold 15. hydroxyapatide form pore scaffold biomimetic scaffold 16. bioreactor 3D scaffold growth factor 17. stem cell D calcium phosphate scaffold cell Biomimetic Platforms for Human Stem Cell Research 18. stem cell Regenerative medicine
  5. 5. , 19 biomimetic stem cell stem cell cell cell cell cell biomimetic biomimetic stem cell (microenviroment), signaling niches stem cell stem cell biomechanic 20 bioreactor scaffold A. hESCs cultured apopotosis bioreactor porous alginate scaffolds B. form embryonic body pore culture stem cell rotating bioreactor scaffold C. D. scaffold cell E. decellulalized scaffold seed stem cell culture in medium perfusion F. bioreactor 21. Craniofacial Bone regeneration , stem cell scaffold-bioreactor system. 22. Engineering anatomically shaped human bone grafts 23. A&B CT TMJ condyles C scaffold trabecular bone MASTERCAM TMJ decellularized scaffold decellularized
  6. 6. D E final scaffold seed stem cell 3 cell 1 scaffold stirred (and precultured statically for 1 week to allow cell attachment, and then the perfusion was applied for an additional 4 weeks.) F culture in vitro bioreactor G-H bioreactor 24. bioreactor 25. cell bioreactor scaffold precultured statically for 1 week to allow cell attachment cell 1 7 cell 5 cell 4.5 perfusion 7.5 culture cell dynamic media cell static bioreactor 10 cell 26. -(A E) trichrome cross section scaffold scaffold static A) - (B perfusion E) F) osteoid ( scaffold - (C and D) Statically statically (B) and in perfusion (F) pore cell - G and H) cultured in perfusion bone lamellae of pore 27. bone reminerlalization μCT matrix 3D pore culture bioreactor matrix static (Scale bar: 5 mm.) 5
  7. 7. 28. (A B) media culture bioreactor TMJ (A) media (B) perfusion C-F) media SEM) (C and D) Flat flow E media flow F) fluid 29. Biomaterials & scaffolds for tissue engineering 30. osteoblast ( collagen-GAG scaffold scaffold 31. A. CG cell infiltration vascularization B. The HA scaffold infiltration vascularization C. CHA porus 32. A infiltration 33. HA collagen B permeability, ( ) HA pore vascularization collagen cell HA scaffold open cell
  8. 8. 34. degradation calvarial defect collagen-calcium phosphate scaffold 4 implant A. degration scaffold B. defect cell 35. In vitro form cell microvessel endothelial cells on the Collagen scaffold. seed 36. ideal scaffold should have several characteristics: (i)high porosity for cell/tissue growth, nutrient diffusion,matrix production and vascularization; (ii) controllable degradation to match tissue growth once implanted in a body and (iii) reasonable mechanical strength to match the tissues at the site of implantation

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