From feather wastes to biofeed


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Biodegradation of feather wastes

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From feather wastes to biofeed

  1. 1. Isolation, Screening and EnzymeProduction of Potent KeratinolyticBacteria from Feather Wastes and its Application Bandana Subedi, Prabin Shakya And Amrit Acharya Microbiological Research Organization Nepal (MiRON)
  2. 2. IntroductionSolid waste management –Discarded in open places –Burning/incineration –Compost –Landfill site –Not well managed
  3. 3. Keratin• Fibrous structural proteins• Recalcitrant• Insoluble• Alpha helix and beta sheets• Sole carbon, nitrogen and sulphur source• Feathers, hair, hoofs etc• Mostly consists of valine, leucine, serine, proline, aspartic and glutamic acid
  4. 4. Protofilament
  5. 5. Keratinase enzyme• Proteolytic endopeptidase enzymes• Produced by some insects and microbes• Mostly serine protease and metalloprotease• Attack on the disulfide (-S-S-) bond• Monomer• Molecular mass range from 20 kDa - 60 kDa• 410-480 residues• Encoded by kerA gene
  6. 6. Keratinase enzyme
  7. 7. Keratinolytic organisms• Bacteria – Bacillus spp. – Arthrobacter spp.• Actinomycetes – Streptomyces spp. – Nocardia spp.• Fungi – Microsporum spp. – Trychophyton spp. – Doratomyces microsporum – Aspergillus fumigatus – Aspergillus flavus
  8. 8. Objectives• Isolation and screening of Keratinolytic Bacteria• Keratinase enzyme production• Partial Purification of produced enzyme• Activity of Keratinase enzyme in different substrates
  9. 9. Methodology• Sample: Soil, feather, Hair and other keratin rich wastes• Screening and Isolation of Potent isolates – Heat treatment – Serial dilution – Plating in selective media• Identification – Morphological Characteristics – Colonial Characteristics – biochemical tests
  10. 10. Methodology….contd.• Enzyme production – batch fermentation process – Feather/Keratin culture broth in shaking in controlled environment shaker• Enzyme Purification – Centrifugation – Ammonium Sulphate Precipitation
  11. 11. Methodology….contd.• Enzyme activity – Hydrolysis of Keratin or other Keratin rich substrates• Determination of Amino acids Released – Thin Layer chromatography / Paper Chromatography• Lab Trial – Enzyme or potent bacterial isolates – Substrates: Keratin, Hair, Feather – Batch Fermentation• Statistical Analysis• Report writing• Report submission• Presentation of findings• Publication
  12. 12. Application• Solid waste management• Biofertilizers• Biofeed• Single cell protien• Amino acids• Biogas• Treatment of prion disease, BSE (Bovine spongiform encephalitis)
  13. 13. Probable findings• Potent Keratinolytic bacteria will be isolated.• Keratinase enzyme will be produced and will be partially purified.• The activity of keratinolytic bacteria and its enzyme on feather will be assessed.• Amino acid released will be evaluated.• Laboratory Trial of the keratin rich solid wastes
  14. 14. Thank you
  15. 15. Questions???