Sergi Fluorescence

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Fluorescence quantification

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Sergi Fluorescence

  1. 1. Fluorescence and Biology Sergi Padilla
  2. 2. George Gabriel Stokes Pink Floyd? microscope
  3. 3. Gin Tonic and Stokes Experiment U.V Light! Quinine found in tonic water !
  4. 5. FRET:
  5. 6. FRET and Lifetime:
  6. 7. Jelly fish glowing in the dark Biological light?
  7. 8. Green Fluorescent Protein out of Aquorea Victoria
  8. 9. Fluorescence decay: A population of a bunch of molecules relaxing
  9. 10.  t  D  DA Fluorescence decay of the donor FRETefficiency  t  D donor Proportion bound donor log I log I
  10. 12. Measurement of the mean lifetime:
  11. 13. <ul><li>. ACQUISITION BEFORE BLEACHING </li></ul><ul><li>. ACQUISITION AFTER BLEACHING </li></ul>A Proof of FRET by FLIM
  12. 14. + + The Nobel prize in Chemistry 2008

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