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Repro protocology 101


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Repro protocology 101

  1. 1. The Evolution and Implementation of Reproductive Synchronization ProtocolsRepro Protocology 101<br />Paul M. Fricke, Ph.D.<br />Professor of Dairy Science <br />University of Wisconsin-Madison<br />
  2. 2. SynchTimeline<br />1980<br />2010<br />2005<br />2000<br />1995<br />1990<br />1985<br />
  3. 3. Manipulating<br />Luteal<br />Function<br />in Cattle<br />
  4. 4. Mechanism of<br />Luteolysis<br />in Cattle<br />1956Hysterectomy was shown to extend luteal lifespan in cattle <br />(Wiltbank and Casida, J AnimSci 15:134-140)<br />
  5. 5. 1967– <br />Removal of the uterine horn ipsilateral to the CL prevented luteal regression, whereas regression occurred normally when the uterine horn contralateral to the CL was removed (Ginther et al., J ReprodFertil 14:225-229)<br />
  6. 6. Conclusions:<br />1) The luteolytic substance originates from the uterus<br />2) Luteolysis is a local rather than a systemic process<br />
  7. 7. A Brief History of Prostaglandin<br />1979– Lutalyse approved for use in cattle by the FDA<br />DinoprostTromethamine<br />Naturally occurring PGF2 in the form of the tromethamine salt<br />Lutalyse® – Pfizer<br />Cloprostenol Sodium<br />Synthetic analog of PGF2 <br />Estrumate® – Intervet<br />
  8. 8. PGF2<br />PGF2<br />PGF2<br />TAI (80h)<br />Targeted Breeding SystemUpjohn - 1980<br />46% CR<br />46% CR<br />4% CR<br />Estrus<br />Estrus<br />14 Days<br />14 Days<br />
  9. 9. 1979<br />Lutalyse<br />approved<br />for use in<br />cattle<br />1980<br />Targeted<br />breeding<br />system<br />SynchTimeline<br />1980<br />2010<br />2005<br />2000<br />1995<br />1990<br />1985<br />
  10. 10. Follicular Function during the Reproductive Cycle<br />Ovarian<br />Follicle<br />Corpus<br />Luteum<br />
  11. 11. New Technology<br />1986<br />O.J. Ginther at Wisconsin<br />J. E. Fortune at Cornell<br />
  12. 12. Progesterone<br />Ovulation<br />Atresia<br />Follicular Size<br />Dominance<br />Deviation<br />Recruitment<br />10<br />Ovulation<br />21<br />Follicular Waves<br />Day After Ovulation<br />
  13. 13. 1979<br />Lutalyse<br />approved<br />for use in<br />cattle<br />1986<br />Follicular<br />waves<br />discovered<br />1980<br />Targeted<br />breeding<br />system<br />Synch Timeline <br />1980<br />2010<br />2005<br />2000<br />1995<br />1990<br />1985<br />
  14. 14. GnRH<br />PGF2<br />GnRH - PGF<br />Twagiramungu et al., 1990<br />6 Days<br />EDAI<br />
  15. 15. 73% of cows detected in estrus on<br />D 7 and 8 in the GnRH – PGF group<br />GnRH<br />or<br />Saline<br />PGF<br />Twagiramungu et al., 1990<br />
  16. 16. Ovsynch<br />Pursley and Wiltbank, 1995<br />GnRH<br />PGF2<br />GnRH<br />TAI<br />7 Days<br />48 h<br />16 h<br />
  17. 17. Ovsynch Schedule<br />
  18. 18. Ovsynch Schedule<br />
  19. 19. Jun, 2001<br />Mar, 2000<br />Distribution of DIM at 1st AI Service:Farm 1<br />DIM at 1st AI<br />Fresh Date<br />
  20. 20. Jun, 2004<br />Nov, 2003<br />Distribution of DIM at 1st AI Service:Farm 2<br />DIM at 1st AI<br />Fresh Date<br />
  21. 21. 1979<br />Lutalyse<br />approved<br />for use in<br />cattle<br />1986<br />Follicular<br />waves<br />discovered<br />1995<br />Ovsynch<br />protocol<br />published<br />1980<br />Targeted<br />breeding<br />system<br />Synch Timeline <br />1980<br />2010<br />2005<br />2000<br />1995<br />1990<br />1985<br />
  22. 22. Pre-Synch<br />Ovsynch<br />Moriera et al., 2001<br />PGF2α<br />TAI<br />GnRH<br />GnRH<br />PGF2α<br />PGF2α<br />7 days<br />16 h<br />12 days<br />56 h<br />14 days<br />
  23. 23. Conception Rate to TAI Ovsynch vs. Presynch – 12 d interval<br />n=262<br />n=272<br />n=264<br />n=278<br />
  24. 24. Presynch/Ovsynch 14/12<br />
  25. 25. Pre-Synch<br />Ovsynch<br />PGF2α<br />TAI<br />GnRH<br />GnRH<br />PGF2α<br />PGF2α<br />EDAI<br />10 to 12 days<br />16 h<br />7 days<br />56 h<br />14 days<br />
  26. 26. Frequency of AI Relative to the Second PGF Injection of Presynch<br />
  27. 27.
  28. 28. 1979<br />Lutalyse<br />approved<br />for use in<br />cattle<br />1986<br />Follicular<br />waves<br />discovered<br />1995<br />Ovsynch<br />protocol<br />published<br />2001<br />Presynch<br />Ovsynch<br />protocol<br />published<br />1980<br />Targeted<br />breeding<br />system<br />Synch Timeline <br />1980<br />2010<br />2005<br />2000<br />1995<br />1990<br />1985<br />
  29. 29. PGF2<br />GnRH<br />GnRH<br />PGF2<br />PGF2<br />Compliance –Presynch/Ovsynch<br />On any given day, 95% of your cows <br />get the correct injection<br />0.95 x 0.95 x 0.95 x 0.95 x 0.95 =77%<br />0.9 x 0.9 x 0.9 x 0.9 x 0.9 =59%<br />
  30. 30. To Resynch or Not to Resynch<br />That is the question….<br />
  31. 31. Presynch/Ovsynch & Resynch<br />
  32. 32. G<br />US+P<br />G+TAI<br />23%<br />(28/120)<br />19<br />26<br />28<br />T<br />T<br />R<br />US+G<br />G+TAI<br />P<br />34%<br />(41/121)<br />26<br />33<br />35<br />T<br />T<br />R<br />US+G<br />G+TAI<br />P<br />38%<br />(54/143)<br />33<br />40<br />42<br />T<br />T<br />R<br />n=711<br />G+TAI<br />0<br />R<br />Fricke et al., J. Dairy Sci. <br />86:3941-3950; 2003<br />
  33. 33. G<br />P<br />G TAI<br />T<br />T<br />R<br />F<br />32<br />39<br />41<br />42<br />Synch and Resynch<br />Presynch/ Ovsynch for first TAI<br />G TAI <br />P<br />G<br />P<br />P<br />R<br />T<br />T<br />R<br />R<br />F<br />0<br />Resynch for nonpregnant cows<br />
  34. 34. 1979<br />Lutalyse<br />approved<br />for use in<br />cattle<br />1986<br />Follicular<br />waves<br />discovered<br />1995<br />Ovsynch<br />protocol<br />published<br />2001<br />Presynch<br />Ovsynch<br />protocol<br />published<br />1980<br />Targeted<br />breeding<br />system<br />2003<br />Resynch<br />protocol<br />comparison<br />published<br />Synch Timeline <br />1980<br />2010<br />2005<br />2000<br />1995<br />1990<br />1985<br />
  35. 35. GnRH<br />PGF<br />GnRH +TAI<br />48 h<br />7 Days<br />GnRH<br />PGF<br />TAI<br />GnRH<br />GnRH<br />PGF<br />GnRH +TAI<br />7Days<br />56 h<br />16 h<br />7 Days<br />72 h<br />Comparison among Cosynch and Ovsynch 56 protocolsBrusveen et al., 2008. J. Dairy Sci. 91:1044-1052.<br />Cows were submitted for TAI after Presynch or as Resynch treatment<br />1507 TAI in 927 lactating Holstein cows<br />
  36. 36. Effect of Treatment on conception rate and pregnancy loss<br />Brusveen et al., 2008. J. Dairy Sci. 91:1044-1052.<br />
  37. 37. 1979<br />Lutalyse<br />approved<br />for use in<br />cattle<br />1986<br />Follicular<br />waves<br />discovered<br />1995<br />Ovsynch<br />protocol<br />published<br />2001<br />Presynch<br />Ovsynch<br />protocol<br />published<br />1980<br />Targeted<br />breeding<br />system<br />2003<br />Resynch<br />protocol<br />comparison<br />published<br />2008<br />Ovsynch56<br />protocol<br />published<br />Synch Timeline <br />1980<br />2010<br />2005<br />2000<br />1995<br />1990<br />1985<br />
  38. 38. Future Directions<br />
  39. 39.
  40. 40.
  41. 41.
  42. 42.
  43. 43.
  44. 44.
  45. 45. Effect of Cyclicity Status on P/AI and Pregnancy Loss after first TAISterry et al., 2006; J Dairy Sci 89:4237<br />n=105<br />n=274<br />
  46. 46. Double Your Pleasure Double Your Fun<br />
  47. 47. Double Ovsynch ScheduleSouza et al., 2009<br />
  48. 48. Effect of synchronization protocol on pregnancies per AI, DIM, lactation number, BCS and locomotion score in lactating dairy cowsSouza et al., Theriogenology 2009 <br />
  49. 49. Effect of treatment onfertility 39 to 45 d after TAISouza et al., 2009<br />n=88<br />n=107<br />n=69<br />n=73<br />
  50. 50. 1979<br />Lutalyse<br />approved<br />for use in<br />cattle<br />1986<br />Follicular<br />waves<br />discovered<br />1990<br />GnRH<br />+<br />PGF<br />protocol<br />published<br />1995<br />Ovsynch<br />protocol<br />published<br />2001<br />Presynch<br />Ovsynch<br />protocol<br />published<br />1980<br />Targeted<br />breeding<br />system<br />2003<br />Resynch<br />protocol<br />comparison<br />published<br />2008<br />Ovsynch56<br />protocol<br />published<br />2009<br />Double<br />Ovsynch<br />protocol<br />published<br />Synch Timeline <br />1980<br />2010<br />2005<br />2000<br />1995<br />1990<br />1985<br />
  51. 51.
  52. 52. 5-Day OvsynchProtocol<br />
  53. 53.
  54. 54. Comparison among Resynch protocolsGiordano et al., unpublished.<br />GnRH<br />PGF<br />TAI<br />GnRH<br />25%<br />n=375<br />7Days<br />56 h<br />16 h<br />GnRH<br />PGF<br />GnRH<br />TAI<br />GnRH<br />31%<br />n=361<br />7Days<br />56 h<br />16 h<br />GnRH<br />PGF<br />hCG<br />TAI<br />GnRH<br />33%<br />n=346<br />7Days<br />56 h<br />16 h<br />d 18<br />d 25<br />d 32<br />d 34<br />d 35<br />