Seminario molecular

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Seminario molecular

  1. 1. EXPRESSION OF FOLLICLE-STIMULATING HORMONE RECEPTOR IN TUMOR BLOOD VESSELS Aurelian Radu,Ph. D, et al Laura Andrea Gómez Marín ID 000144738 Felipe Lozano Pineda ID 000172651 Molecular Biology Medicine school III semester
  2. 2. INTRODUCTION
  3. 3. FSH Is a hormone that is produced in the anterior pituitary gland. In females: FSH stimulates follicular maduration and estrogen . In males: FSH stimulates Sertolli cell proliferation spermatogenesis
  4. 4. TUMOR
  5. 5. They are anormal celular proliferation at the blood vassels cells BLOOD VESSELS TUMOR
  6. 6. http://www.google.com.co/images?hl=es&biw=1276&bih=557&gbv=2&tbs=isch%3A1&sa=1&q=BLOOD+VASSELS+TUMOR&aq=f&aqi=&aql=&oq= BLOOD VESSELS TUMOR CHARACTERISTICS
  7. 7. FSH receptor is known to be expressed only in the testicular sertolli cells and the ovarian granulosa cells. But acording to the study the FSH positive receptor expression was localizated in blood vessels surrounding the tumor (EX: breast , prostate and testis tumor) In consecuence FHS can stimulate the proliferartion and maturation of those tumor cells. RELATIONSHIP BETWEEN FSH AND TUMOR
  8. 8. GENERAL OBJECTIVE Identify the expression of follicule-stimulating Hormone receptor in tumor blood vessels Imagen de www.google.com
  9. 9. MATERIALES Y MÉTODOS
  10. 10. MUESTRAS Y ANTICUERPOS
  11. 11. FISH Y MICROSCOPIA CONFOCAL Fish cDNA antisense oligonucleotide Microscopia confocal Imagen de: www.google.com
  12. 12. INMUNOPRECIPITACIÓN, ELECTROFORESIS Y WESTERN BLOT Imagen de: www.molecular station.com
  13. 13. RESULTADOS
  14. 14. <ul><li>FSHR323 </li></ul><ul><li>Ac monoclonal </li></ul><ul><li>Receptor FSH +/FSH – </li></ul><ul><li>IgG2a/ NO TINCION </li></ul><ul><li>V. linfaticos(D2-40) </li></ul>RESULTADOS
  15. 15. IDENTIFICACIÓN RECEPTOR DE FSH RESULTADOS
  16. 16. <ul><li>Banda 87 Kd </li></ul><ul><li>Hibridación in situ </li></ul><ul><li>“ Improbable que otra proteína tenga dos epitopos y el mismo peso que el receptor de FSH” </li></ul>Expresión de RNA de receptor de FSH RESULTADOS
  17. 17. RESULTADOS
  18. 18. RESULTADOS Expresión del receptor en otros canceres Imagen de: www.google.com
  19. 19. RESULTADOS
  20. 20. RESULTADOS
  21. 21. DISCUSSION
  22. 22. DISCUSSION Author What he say Agree or disagree Mariani S,Ben –Josef, et al. “ two previous studies showed immunohistochemical detection of the FSH receptor in the tumor cells of prostate adenocarcinomas” Alam H, et al. “ the binding of FSH to FSH receptor in ovarian granulosa cell induces an increase in hypoxia-inducible factor 1alfa protein levels under normoxic conditions, which in turn leads to up-regulation of vascular endothelial growth factor(VEGF)”
  23. 23. DISCUSSION Author What he say Agree or disagree Fox SB, et al. “ another possibbility is suggested by the observation that in the breast tumors in humans, endothelial cells proliferate at the tumor periphery but not in the interior” Castro-Fernández C, et al. “ the fact that FSH and FSH receptor signaling is known to generate activated Gq/11 protein”
  24. 24. PERSONAL CONCLUSIONS <ul><li>The possibility to know specifically the expression of TSH receptor will allow doctors improve techniques in order to reduced the risk of metastasis in blood vessels cells. </li></ul><ul><li>Thanks to the developing of inmublotting, inmunohistochemical and the FSH-receptor purified antibodies techniques many diseases can be detected faster, in that way patients would have more chances to survive </li></ul>
  25. 25. PERSONAL CONCLUSIONS <ul><li>This kind of investigations give more alternatives on diagnosis and treatment to the medical society in order to focus properly each of different diseases existing </li></ul><ul><li>The topic was interesting, also is something that we are learning at this moment so that aspect made us the workshop more understandable and more applicable to our low knowledge in medicine. </li></ul>
  26. 27. MAPA CONCEPTUAL FELIPE
  27. 28. http://www.molecularstation.com/es/protein/immunoprecipitation/ http://www.uco.es/grupos/inmunologiamolecular/inmunologia/tema17/etexto17.htm MARTINEZ S., Lina María. Biología molecular. 5. ed. Medellín: UPB. Fac. de Medicina, 2009. 313 p. (Notas de clase: biología molecular). LUQUE CABRERA, J y HERRAEZ SANCHEZ, A. Texto ilustrado de Biología Molecular e Ingeniería genética: conceptos, técnicas y aplicaciones en ciencias de la salud. Madrid: Elsevier Science, 2001. 469p BIBLIOGRAFÍA

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