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OrganoLinx ViaGlue Introduction

OrganoLinx ViaGlue Introduction

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OrganoLinx ViaGlue Introduction

  1. 1. Examples of Cell Assembly Complex Spheroid Formation Functional 3!) Liver Tissue ~ ‘ O - 1. “V, -‘ Rapid and Status 3DLIver1’xssue no —a-*< ‘ yum-u c. - &~o~~&~~ . ... ... .. . . j ‘C —- 1; i—» . _.-. - . ... ..M. . rut fufluru
  2. 2. « ‘ Examples of Various Cell Assemblies Generated by ViaGlue
  3. 3. Functional 3D Cardiac Tissue with ViaGlue j, : ":Ol, ;ixL: HX F: l.wrv; il. ~i. ~:-31:. HUVECS C. ;ud| on'iyocytes ‘l l -2 ‘an , i . i3 l , Jr ViaGlue 8 3D Co—Culture Cardiac Tissue 2DCo-Culture $3: l
  4. 4. i, ’ v -, . . "‘ Ol‘fjPlrl(DLlllX 3D Cardiac Tissue Assembled with ViaGlue '4 change (increase) ‘in me rate al beouig 5i change (decrease) In me me 9! bearing Jun MD » ) ll) ‘:0 -K) 20 10 i i 5500 iwpmiaiiiio T00uM Do: oruhiririr l0riM hopunailmu ?00uM l)0l0'Uhl( int‘ 20 l'. .l! illuTViyiV(‘11P‘_ Jnmii ID i I'r1l-’-rTiy. ‘(y1f0'. ll) Vllll . '[J . .iiiii; ,n. y.-, i um :0 mi. in (. Iliii(vlViyr)(y1ll“i ll’) mi- 8 20 Cardlornyocytes 30 Cardiomyocyles . . , ..? E"E"¥ "~‘»: ?‘” ‘ 7' '~": '7?"""? £""' "I-'7 ’; _U. “ is" - ' lr Fluorescent antibody staining of tissues for expression of cardiac markers (cardiac troponin T and (onnexln 43]. Scale bar; 8DpM * DAPI cTnT '—' Cam 43
  5. 5. Various Cardiac Tissue Assemblies Spontaneously Beat (no external electrical stimulation) Scaffold free ViaGlue allows high cell contact density for large tissue synchronous beating ZD Cdfdl0lYvl)(. 'y'llJ' 092* . i l| lHll, llll'l| [ll ll) Caidioriiyocyles 0 {ids ll. ‘ ‘.1 l | l?lH“l. l«l| ‘ll1‘| |llllll I-opmuiinn in nos) 20 Mar (1.78) 3D Mix 0.835 Tune mcrval for tissue boats "-. i'— M Oi'g{*. rit: iLinX
  6. 6. ii: 10" OrganoLinX A Dual Receptor and Reporter for Multi-Modal Cell Surface Engineering. W. Luo, N. P. Westcott, D. Dutta, A. Pulsipher, D. Rogozhnikov, J. Chen, M. N. Vousaf‘. ACS Chemical Biology, 2015, 10, 2219-2226. Spheroid and Tissue Assembly in Microfluidic Flow. PJ. O'Brien, W. Luo, D. Rogoznikov, J. Chen, M. N. Vousai‘. Biooonjugate Chem. 2015, 26, 19394949 Bioorthogonal oxime ligation mediated in vivo cancer targeting. Li Tang et al. Chem. Sci. , 2015, 6, 2182-2186 Remote Control of Tissue Interactions via Engineered Photo-switchable Cell Surfaces. W. Luo, A. Pulsipher, D. Dutta, B. M. Lamb, M. N. Yousaf‘. Nature Scientific Reports 2014, 4, 6313. Cell surface engineering by a conjugation and release approach based on the formation and cleavage oi oxime linkages upon mild electrochemical oxidation and reduction. A. Pulsipher, D. Dutta, W. Luo, M. N. Yousaf‘. Angew. Chem. Int. Ed. 2014, 53, 9487-9492. Directing Neuronal Signaling through Cell-Surface Glycan Engineering. A. Pulslpher, M. E. Grlfiin, S. E. Stone, J. M. Brown, and L C. Hsieh-Wilson J. Am. Chem. Soc. , 2014, 136, 6794-6797. Engineering Cell Surfaces via Liposome Fusion. 0. Dutta, A. Pulsipher, W. Luo, M. N. Yousaf‘. Bioconjugate Chemistry. 2011, 22, 2423-2433. Chemical 8: Engineering News 2011, 49, 36. Synthetic Chemoselective Rewiring of Cell Surfaces: Generation of Three-Dimensional Tissue Structures. D. Dutta, A. Pulsipher, W. Luo, M. N. Yousaf'. J. Am. Chem. Soc. 2011, 133, 8704-8713. Science 2011, 332, 1011-1012.

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