Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.
My
Presentation
• DNA?
• Structure of DNA
• Method of DNA isolation
• Maxwell® 16 Plant DNA Kit Method
• Spin column Method
• CTAB Method
...
DNA?
Something to say about DNA
Cell Nucleus is the
empire DNA
Structure of DNA
Adenine-Thymine
Base Pair
Guanine-Cytosine
Base Pair
1. Maxwell® 16 Plant DNA Kit
Method
2. Spin column Method
3. CTAB Method
Method of DNA isolation
Ridge end
Label end
Contents
Lysis Buffer
MagneSil PMPs
Wash Buffer
Wash Buffer
Wash Buffer
Wash Buffer
Wash Buffer
Plunge...
Spin column Method
It is a solid phase extraction method
Quickly purify nucleic acids.
Nucleic acid will bind to the so...
CTAB Method
Detergent+
Lipid & Proteins Detergent
DNA
CTAB Stands for
cetyl trimethyl ammonium bromide
oCTAB
oChloroform
oIsoamyl alchohol
oIsopropyl alcohol
oRNAse
oQiagen
oAmbion
o Proteinase K
oNaCl
oMercaptoethanol
oAmmon...
Procedure for CTAB method
Sample extract
CATB Centrifuge Carbohydrates,
proteins, etc.
Nucleic acid-
CTAB complex
DNA
Prec...
Why DNA Extraction?
• Genetic Testing
Body Identification
• Analysis of Forensic Evidence
There are four temperature based strategies for long term DNA
conservation. These are storage at:
 -20° C
 -80° C
 -196...
Understanding Individuality of Plants
Using genetic information of DNA for plant modification
 Use in forensic analysis...
Plant dna extraction method
Plant dna extraction method
Plant dna extraction method
Upcoming SlideShare
Loading in …5
×

Plant dna extraction method

This is about extraction of DNA from plant source.

  • Login to see the comments

Plant dna extraction method

  1. 1. My Presentation
  2. 2. • DNA? • Structure of DNA • Method of DNA isolation • Maxwell® 16 Plant DNA Kit Method • Spin column Method • CTAB Method • Reagent Used In CTAB Method • Procedure for CTAB method • Why DNA Extraction? • Storage of isolated DNA • Use of the DNA
  3. 3. DNA? Something to say about DNA Cell Nucleus is the empire DNA
  4. 4. Structure of DNA Adenine-Thymine Base Pair Guanine-Cytosine Base Pair
  5. 5. 1. Maxwell® 16 Plant DNA Kit Method 2. Spin column Method 3. CTAB Method Method of DNA isolation
  6. 6. Ridge end Label end Contents Lysis Buffer MagneSil PMPs Wash Buffer Wash Buffer Wash Buffer Wash Buffer Wash Buffer Plunger Reagent Cartridge Maxwell® 16 Plant DNA Kit Method  An easy method efficient  Automated purification of genomic DNA from plant tissue samples.
  7. 7. Spin column Method It is a solid phase extraction method Quickly purify nucleic acids. Nucleic acid will bind to the solid phase of silica under certain conditions.
  8. 8. CTAB Method Detergent+ Lipid & Proteins Detergent DNA CTAB Stands for cetyl trimethyl ammonium bromide
  9. 9. oCTAB oChloroform oIsoamyl alchohol oIsopropyl alcohol oRNAse oQiagen oAmbion o Proteinase K oNaCl oMercaptoethanol oAmmonium acetate
  10. 10. Procedure for CTAB method Sample extract CATB Centrifuge Carbohydrates, proteins, etc. Nucleic acid- CTAB complex DNA Precipitated Nucleic acid-CTAB complex
  11. 11. Why DNA Extraction? • Genetic Testing Body Identification • Analysis of Forensic Evidence
  12. 12. There are four temperature based strategies for long term DNA conservation. These are storage at:  -20° C  -80° C  -196° C  Dried, at room temperature Store DNA in dry state, reduces molecular mobility and inhibits the depurination, depyrimidination, deamination and hydrolytic The DNA immobilized on FDA cards is amenable to PCR after 17 years storage. DNA in aqueous phase is stored under slightly basic conditions. At -196° C, DNA is maintained in a vitreous state General consideration for DNA storage:
  13. 13. Understanding Individuality of Plants Using genetic information of DNA for plant modification  Use in forensic analysis Use to solve historical puzzle

×