On the subject of splitting clades, another interesting trend also fell out when we compared similar species from different continents. This list represents all species in our sample that were common to both Australian and South African waters. The names highlighted in green text represent offshore pelagics, while the red indicates those fishes which are tied to the reef. Strikingly there is a lot more divergence within species that are reef-associated for the duration of their adult life and rely on dispersive larvae to cross large oceans. These results suggest that the Indian Ocean may be a more effective barrier to gene flow for these fishes than was once thought. These results are counter to the idea that dispersive larvae keep the world’s oceans connected.
In closing, I would like to acknowledge the deep involvement of the whole DNA barcode group at Guelph in honing the protocols that I have discussed. If you have questions, I will try to answer them.
8 fish bol 5
Barcoding Marine Fishes: A Three-Ocean Perspective
Barcoding All Marine Fishes – 15 000 Species Target = 15 000 species Barcoding All Marine Fishes
Recovering Barcodes from Fishes Taxonomic Coverage 530 Species (3.5%) 317 Genera (10.5%) 130 Families (32%) 28 Orders (55%) 5’ 3’ 648-bp Fish F1 Fish F2 Fish R2 Fish R1 Fish Forward Fish Reverse
Distribution Map Pacific Indian Atlantic Collection Site Canada Australia South Africa Portugal
Conclusions <ul><li>Highly conserved priming regions </li></ul><ul><li>High species resolution within each region </li></ul><ul><li>Species discovery through DNA barcodes </li></ul><ul><li>Specimen vouchering </li></ul>
Collaborators Paul Hebert Bob Ward Allan Connell Jim Boutillier Filip é Costa Bronwyn Innes
Acknowledgments Acknowledgements Laboratory Database Collections Funding & Support Jeremy deWaard Sujeevan Ratnasingham Jim Boutillier Gordon and Betty Moore Foundation NSERC CFI OIT Canada Research Chairs Program Rob Dooh Janet Topan Nataly Ivanova Angela Holliss Allan Connell Peter Last Pia Marquardt