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The
mission
ImpossibleII
JSTP 12
The
mission
ImpossibleII
JSTP 12
The
mission
Impossible II
The toy story
Impossible
The
mission
Impossible
Project
โครง
โครง
โครงโครงงาน
โครงโครงงาน
โครงโครงงาน
Project
PROJECT = TOY
GAME
FUN
PROJECT = TOY
GAME
FUN
แบบฟอร์ม
โครงงาน
ที่มาและความสำคัญ
วัตถุประสงค์ประโยชน์ที่คาดว่าจะได้รับตัวแปรที่เกี่ยวข้อง
คู่มือการเล่น
เล่นอย่างไรให้สนุก
คู่มือการเล่น
เล่นอย่างไรให้สนุก
อ่านจบแล้ว
จะสนุกไหม
Every years someone
has a great speech....
@#!$#%wee-tah-kah
loo-loo
คันหูจุงบ่องตง
ต่อยอด
ต่อยอด
สร้างยาลดอาการ
คันหูของFurby
Fin~
Innovation
What did we play ?
Detective
What did we play ?
Detective Created
What did we play ?
Detective Created Divas
What did we play ?
Detective Created Divas
Action
What did we play ?
Detective Created Divas
Business
Action
What did we play ?
Detective Created Divas
Impersonate
Business
Action
What did we play ?
Detective Created Divas
Impersonate
Business
Action
What did we play ?
ซ่อนแอบ
ไขปริศนา
Detective Created Divas
Impersonate
Business
Action
What did we play ?
ซ่อนแอบ
ไขปริศนา
เล่น
Lego
พับจรวด
หุ่นยนต์
Detective Created Divas
Impersonate
Business
Action
What did we play ?
ซ่อนแอบ
ไขปริศนา
เล่น
Lego
พับจรวด
หุ่นยนต์
ร้องเพล...
Detective Created Divas
Impersonate
Business
Action
What did we play ?
ซ่อนแอบ
ไขปริศนา
เล่น
Lego
พับจรวด
หุ่นยนต์
ร้องเพล...
Detective Created Divas
Impersonate
Business
Action
What did we play ?
ซ่อนแอบ
ไขปริศนา
เล่น
Lego
พับจรวด
หุ่นยนต์
ร้องเพล...
Detective Created Divas
Impersonate
Business
Action
What did we play ?
ซ่อนแอบ
ไขปริศนา
เล่น
Lego
พับจรวด
หุ่นยนต์
ร้องเพล...
P.P. TOYs
P.P. TOYs
P.P. TOYs
P.P. TOYs
P.P. TOYs
P.P. TOYs
Bacteria
Bacteria
Styrofoam
Biodegradation
Bacteria
Styrofoam
My project in academic style.
Styrofoam
Styrofoam
Styrene
monomer
Polystyrene
Chemical formula is (C8H8)n
Monomer styrene
Thermoplastic
Blowing agents
Polystyrene
Chemical formula is (C8H8)n
Monomer styrene
Thermoplastic
Blowing agents
500 years++
for degradation
http://en...
Recycling &
Incineration
Recycling &
Incineration
1%
turn back to polystyrene
most of the polystyrene is converted
into carbon dioxide, water vapor...
Photo by Napat Jaitui JSTP # 15
Styrene
Monomer
Carbon
Monoxide
Photo by Napat Jaitui JSTP # 15
Styrene
Monomer
Carbon
Monoxide
Photo by Napat Jaitui JSTP # 15
CARCINOGEN
c
c
c c
c
c
c c
c
c
c c
c
c
c c
Bacteria
nutrition
‣ Energy source
‣ Carbon source
‣ Nitrogen source
‣ Minerals
‣ Water
‣ Growth factors
Howcan I find
the bacteria that able to
degrade a styrofoam?
SOIL
SOIL
SOIL
Landfill
Landfill
foam
Howcan I find
the bacteria that able to
degrade a styrofoam?
Howcan I find
the bacteria that able to
degrade a styrofoam?
The
ability
The key to isolate the
effective microbe is the
degradability.
MSM Broth
Bacteria selective broth
MSM Broth
Bacteria selective broth
MSM broth
‣K2HPO4
‣KH2PO4
‣(NH4)2SO4
‣MgSO4
‣ FeSO4.2HO2
‣MnCl2.4H2O
‣CoCl2.6H2O
‣CuCl2.2H2O
‣NiCl2.6H2O
‣Na2MoO4.2H2O
‣Zn...
MSM Broth
Bacteria selective broth
Shake in shaker for 1 month
then inoculate to new fresh
broth for sub culture.
Every week the solution in each flask was taken
to the eppendorf then stored at 2C๐ for stop bacteria
growth that use for ...
MSM Broth
Bacteria selective broth
MSM Broth
Bacteria selective broth
MSM broth
‣K2HPO4
‣KH2PO4
‣(NH4)2SO4
‣MgSO4
‣ FeSO4.2HO2
‣MnCl2.4H2O
‣CoCl2.6H2O
‣CuCl2.2H2O
‣NiCl2.6H2O
‣Na2MoO4.2H2O
‣Zn...
MSM Broth
Bacteria selective broth
MSM Broth
Bacteria selective broth
MSM Broth
Bacteria selective broth
MSM Broth
Bacteria selective broth
Foam in landfill Soil in landfill
Week
Week
Week
Dominant
species
Week
Dominant
species
Diversity
Every week the solution in each flask was taken
to the eppendorf then stored at 2C๐ for stop bacteria
growth that use for ...
DNA Replication : PCR (TopTaq Master Mix Kit)
16S rRNA gene Amplification
by using Primer VR (Medlin et al., 1998) & VFC (...
DNA Replication : PCR (TopTaq Master Mix Kit)
16S rRNA gene Amplification
by using Primer VR (Medlin et al., 1998) & VFC (...
DNA Replication : PCR (TopTaq Master Mix Kit)
16S rRNA gene Amplification
by using Primer VR (Medlin et al., 1998) & VFC (...
DGGE (Denature Gradient Gel Electrophoresis)
30%
Urea + Formamine
60%
Urea + Formamine
DGGE (Denature Gradient Gel Electrophoresis)
- 30%
Urea + Formamine
60%
Urea + Formamine
+
DGGE (Denature Gradient Gel Electrophoresis)
30%
Urea + Formamine
60%
Urea + Formamine
DGGE (Denature Gradient Gel Electrophoresis)
30%
Urea + Formamine
60%
Urea + Formamine
DGGE (Denature Gradient Gel Electrophoresis)
30%
Urea + Formamine
60%
Urea + Formamine
DGGE (Denature Gradient Gel Electrophoresis)
30%
Urea + Formamine
60%
Urea + Formamine
GC rich
DGGE (Denature Gradient Gel Electrophoresis)
30%
Urea + Formamine
60%
Urea + Formamine
GC low
GC rich
DGGE (Denature Gradient Gel Electrophoresis)
30%
Urea + Formamine
60%
Urea + Formamine
GC low
GC rich
Marker
Soilweek1
Soilweek5
Soilweek6
Soilweek7
Soilweek8
Soilweek20
Foamweek1
Foamweek5
Foamweek6
Foamweek7
Foamweek8
Foam...
Marker
Soilweek1
Soilweek5
Soilweek6
Soilweek7
Soilweek8
Soilweek20
Foamweek1
Foamweek5
Foamweek6
Foamweek7
Foamweek8
Foam...
Marker
Soilweek1
Soilweek5
Soilweek6
Soilweek7
Soilweek8
Soilweek20
Foamweek1
Foamweek5
Foamweek6
Foamweek7
Foamweek8
Foam...
Marker
Soilweek1
Soilweek5
Soilweek6
Soilweek7
Soilweek8
Soilweek20
Foamweek1
Foamweek5
Foamweek6
Foamweek7
Foamweek8
Foam...
Marker
Soilweek1
Soilweek5
Soilweek6
Soilweek7
Soilweek8
Soilweek20
Foamweek1
Foamweek5
Foamweek6
Foamweek7
Foamweek8
Foam...
Marker
Soilweek1
Soilweek5
Soilweek6
Soilweek7
Soilweek8
Soilweek20
Foamweek1
Foamweek5
Foamweek6
Foamweek7
Foamweek8
Foam...
The microscopic structure of
Polystyrene observed by SEM
Pat Pataranutaporn1
, Apinya.J2
Assistant prof. Savaporn Supaphol...
Control : Polystyrene in MSM broth without bacterial source.
Regular polystyrene foam that didn’t use in experiment.
100x ...
Regular polystyrene that didn’t use in experiment.
Control
Polystyrene in Medium with bacteria from foam sample.
100x 200x 500x
The comparison of Polystyrene microscopic structure
R...
Polystyrene in Medium with bacteria from foam sample.
100x 200x 500x
The comparison of Polystyrene microscopic structure
R...
Polystyrene in Medium with bacteria from soil sample.
100x 200x 500x
The comparison of Polystyrene microscopic structure
R...
Polystyrene in Medium with bacteria from soil sample.
100x 200x 500x
The comparison of Polystyrene microscopic structure
R...
Polystyrene in Medium with bacteria from soil sample.
100x 200x 500x
The comparison of Polystyrene microscopic structure
R...
Polystyrene in Medium with bacteria from soil sample.
100x 200x 500x
The comparison of Polystyrene microscopic structure
R...
Soilweek1
Soilweek5
Soilweek6
Soilweek7
Soilweek8
Foamweek1
Foamweek5
Foamweek6
Foamweek7
Foamweek8
Controlweek6
Controlwe...
Soilweek1
Soilweek5
Soilweek6
Soilweek7
Soilweek8
Foamweek1
Foamweek5
Foamweek6
Foamweek7
Foamweek8
Controlweek6
Controlwe...
Soilweek1
Soilweek5
Soilweek6
Soilweek7
Soilweek8
Foamweek1
Foamweek5
Foamweek6
Foamweek7
Foamweek8
Controlweek6
Controlwe...
Soilweek1
Soilweek5
Soilweek6
Soilweek7
Soilweek8
Foamweek1
Foamweek5
Foamweek6
Foamweek7
Foamweek8
Controlweek6
Controlwe...
Molecular cloning
DNA isolation & DNA Analyzing
Pat Pataranutaporn1
, Sureeporn Nualkaew2
, Prof. Dr. Amornrat Phongdara2
...
Marker
Soilweek1
Soilweek5
Soilweek6
Soilweek7
Soilweek8
Soilweek20
Foamweek1
Foamweek5
Foamweek6
Foamweek7
Foamweek8
Foam...
Marker
Soilweek1
Soilweek5
Soilweek6
Soilweek7
Soilweek8
Soilweek20
Foamweek1
Foamweek5
Foamweek6
Foamweek7
Foamweek8
Foam...
Marker
Soilweek1
Soilweek5
Soilweek6
Soilweek7
Soilweek8
Soilweek20
Foamweek1
Foamweek5
Foamweek6
Foamweek7
Foamweek8
Foam...
Molecular cloning
Despicable Me: Minion Mayhem
PCR Product
PCR Product
PCR Product
Mix DNA for ligation
pGEM- T easy
Target DNA
PCR Product
Mix DNA for ligation
PCR Product
Mix DNA for ligation
Transform plasmid to the competent cell.
E.Coli as
Transform plasmid to the competent cell.
Transform plasmid to the competent cell.
Transform plasmid to the competent cell.
Transform plasmid to the competent cell.
Transform plasmid to the competent cell.
The separate DNA
Marker
Soilweek1
Soilweek5
Soilweek6
Soilweek7
Soilweek8
Soilweek20
Foamweek1
Foamweek5
Foamweek6
Foamweek7
Foamweek8
Foam...
Electropherogram
Code name : Control 4
Code name : Control 4
Basic Local Alignment Search Tool (BLAST)
Database Name TL/16S_ribosomal_RNA_Bacteria_and_Archaea
De...
Code name : Control 4
Basic Local Alignment Search Tool (BLAST)
Database Name TL/16S_ribosomal_RNA_Bacteria_and_Archaea
De...
Code name : Control 4
Phylogenetic tree
Code name : Control 4
Phylogenetic tree
Code name : Control 4
Phylogenetic tree
Electropherogram
Code name : Control 7
Code name : Control 7
Basic Local Alignment Search Tool (BLAST)
Database Name TL/16S_ribosomal_RNA_Bacteria_and_Archaea
De...
Code name : Control 7
Basic Local Alignment Search Tool (BLAST)
Database Name TL/16S_ribosomal_RNA_Bacteria_and_Archaea
De...
Code name : Control 7
Phylogenetic tree
Code name : Control 7
Phylogenetic tree
Code name : Control 7
Phylogenetic tree
Code name : Fe5
Bacterial plating
Plate streaking & Plate spreading
Pat Pataranutaporn1
, Panwong Kuntanawat2
1
PSU.Wittayanusorn school, ...
Bacterial plating on MSM & MSM + PSA
agar cultured at 37o
C over 48 hr.
Bacterial plating on MSM & MSM + PSA
agar cultured at 37o
C over 48 hr.
Bacterial plating on MSM & MSM + PSA
agar cultured at 37o
C over 48 hr.
Polystyrene-coacrylic acid (PSA)
(particles diamet...
Bacterial plating on MSM & MSM + PSA
agar cultured at 37o
C over 48 hr.
Polystyrene-coacrylic acid (PSA)
(particles diamet...
Bacterial plating on MSM & MSM + PSA
agar cultured at 37o
C over 48 hr.
Bacterial plating on MSM & MSM + PSA
agar cultured at 37o
C over 48 hr.
Bacterial plating on MSM & MSM + PSA
agar cultured at 37o
C over 48 hr.
Bacterial plating on MSM & MSM + PSA
agar cultured at 37o
C over 48 hr.
Research
Achievements
Research
Achievements
Research
Achievements
13th
NCSC, Jaipur India 2011
Youth summit 2012, Dubai UAE
JSTP Scholarship
STT 36
BYEE, Leverkuzen G...
Publication & media
JSTPMedia
www.jstpmedia.org
Biodegradation of
Polystyrene Foam by
the Microorganisms
from Landfill
P.P. TOYs
P.P. TOYs
หนังตะลุง
หนังตะลุง
WEB app
หนังตะลุง
WEB app
Culture
Developed in SWF format
http://jstpmedia.org/images/complete.swf
A S ◀ ◀
◀
◀
move to the next character
Head(Speak)
Left hand right hand
Body flip
Demo
JSTP 15/3
P.P. TOYs
P.P. TOYs
Space
Space
Gravity
Space
Enzyme
Gravity
JAXA
v
v
The Evaluation of Amylase
Activity under Microgravity
By JSTP 12
Pat Pataranutaporn
Pondet Ananchai
Arnan Schuett
ChemistryBiotechnology Electronic
Research Team
JSTP 12
ChemistryBiotechnology Electronic
Research Team
JSTP 12
The youngest team
Possibility of Enzyme
Possibility of Enzyme
Collision Theory
Possibility of Enzyme
Collision Theory
‣Enzyme Concentration
‣Substrate Concentration
‣Inhibitors
‣Temperature
‣pH
Gravity
GravityFree motion of molecule
Salivary stress markers and psychological stress in
simulated microgravity: 21 days in 6° head-down tilt.
Rai B, Kaur J.
C...
Difference enzyme activity
rate affected by gravity
Enzyme containing
Enzyme containing
Pumping system
Enzyme containing
Pumping system
Starch measuring
EnzymeamylaseStarchAcedicacid
Enzyme containing
Pumping system
Starch measuring
EnzymeamylaseStarchAcedicacid
Enzyme containing
Pumping system
Starch measuring
EnzymeamylaseStarchAcedicacid
Enzyme containing
Pumping system
Starch measuring
2
EnzymeamylaseStarchAcedicacid
Enzyme containing
Pumping system
Starch measuring
24 2
EnzymeamylaseStarchAcedicacid
Enzyme containing
Pumping system
Starch measuring
246 2 24
EnzymeamylaseStarchAcedicacid
Enzyme containing
Pumping system
Starch measuring
2468 2 2 24 46
EnzymeamylaseStarchAcedicacid
Enzyme containing
Pumping system
Starch measuring
2468 2 2 2 04 46
EnzymeamylaseStarchAcedicacid
Enzyme containing
Pumping system
Starch measuring
2468 2 2 2 04 46
EnzymeamylaseStarchAcedicacid
Enzyme containing
Pumping system
Starch measuring
2468 2 2 2 04 46
EnzymeamylaseStarchAcedicacid
Enzyme containing
Pumping system
Starch measuring
2468 2 2 2 04 46
low starch remain High st...
026 48
026 48
starch remain
(%v/w)
026 48
starch remain
(%v/w)
Time (s) 026 48
starch remain
(%v/w)
Time (s) 026 48
starch remain
(%v/w)
Time (s) 026 48
starch remain
(%v/w)
Time (s) 026 48
starch remain
(%v/w)
Time (s) 026 48
Y
starch remain
(%v/w)
Time (s) 026 48
Y
X
starch remain
(%v/w)
Time (s) 026 48
Y
X
Rate =
Working period
Working period
2 moths for discussing
Working period
2 moths for discussing
3 hours for proposal
Working period
2 moths for discussing
3 hours for proposal
1 night for presentation
Working period
2 moths for discussing
3 hours for proposal
1 night for presentation
5 minutes for presentation rehearsing
JSTP Team
JSTP Team
ไม่มี limit ชีวิตเกินร้อย
PP’s TOYP.P. TOYs
PP’s TOYP.P. TOYs
What you
are seeing
now.
Presentation
DESCIPost modern
interface
Available at
JSTPMedia
soon !
!
You have a chance
to answer your
own question .
ของเล่นที่สนุก
เราต้องเลือกเอง
“ Never be afraid to asked “
POSSIBLEIM
AKE POSSIBLEI M
Right now...
It’s your
turn
It’s your
turn
THANK YOU
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
Mission impossible II
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Mission impossible II

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Mission impossible II

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Mission impossible II

  1. 1. The mission ImpossibleII JSTP 12
  2. 2. The mission ImpossibleII JSTP 12
  3. 3. The mission Impossible II The toy story
  4. 4. Impossible The mission
  5. 5. Impossible Project
  6. 6. โครง
  7. 7. โครง
  8. 8. โครงโครงงาน
  9. 9. โครงโครงงาน
  10. 10. โครงโครงงาน Project
  11. 11. PROJECT = TOY GAME FUN
  12. 12. PROJECT = TOY GAME FUN
  13. 13. แบบฟอร์ม โครงงาน ที่มาและความสำคัญ วัตถุประสงค์ประโยชน์ที่คาดว่าจะได้รับตัวแปรที่เกี่ยวข้อง
  14. 14. คู่มือการเล่น เล่นอย่างไรให้สนุก
  15. 15. คู่มือการเล่น เล่นอย่างไรให้สนุก อ่านจบแล้ว จะสนุกไหม
  16. 16. Every years someone has a great speech....
  17. 17. @#!$#%wee-tah-kah loo-loo
  18. 18. คันหูจุงบ่องตง
  19. 19. ต่อยอด
  20. 20. ต่อยอด สร้างยาลดอาการ คันหูของFurby
  21. 21. Fin~
  22. 22. Innovation
  23. 23. What did we play ?
  24. 24. Detective What did we play ?
  25. 25. Detective Created What did we play ?
  26. 26. Detective Created Divas What did we play ?
  27. 27. Detective Created Divas Action What did we play ?
  28. 28. Detective Created Divas Business Action What did we play ?
  29. 29. Detective Created Divas Impersonate Business Action What did we play ?
  30. 30. Detective Created Divas Impersonate Business Action What did we play ? ซ่อนแอบ ไขปริศนา
  31. 31. Detective Created Divas Impersonate Business Action What did we play ? ซ่อนแอบ ไขปริศนา เล่น Lego พับจรวด หุ่นยนต์
  32. 32. Detective Created Divas Impersonate Business Action What did we play ? ซ่อนแอบ ไขปริศนา เล่น Lego พับจรวด หุ่นยนต์ ร้องเพลง แต่งตัว
  33. 33. Detective Created Divas Impersonate Business Action What did we play ? ซ่อนแอบ ไขปริศนา เล่น Lego พับจรวด หุ่นยนต์ ร้องเพลง แต่งตัว เล่นเกม แปลงร่าง
  34. 34. Detective Created Divas Impersonate Business Action What did we play ? ซ่อนแอบ ไขปริศนา เล่น Lego พับจรวด หุ่นยนต์ ร้องเพลง แต่งตัว เกมเศรษฐี เล่นขายของ เล่นเกม แปลงร่าง
  35. 35. Detective Created Divas Impersonate Business Action What did we play ? ซ่อนแอบ ไขปริศนา เล่น Lego พับจรวด หุ่นยนต์ ร้องเพลง แต่งตัว บทบาทสมมุติ หมอรักษาคนไข้ เกมเศรษฐี เล่นขายของ เล่นเกม แปลงร่าง
  36. 36. P.P. TOYs
  37. 37. P.P. TOYs
  38. 38. P.P. TOYs
  39. 39. P.P. TOYs
  40. 40. P.P. TOYs
  41. 41. P.P. TOYs
  42. 42. Bacteria
  43. 43. Bacteria Styrofoam
  44. 44. Biodegradation Bacteria Styrofoam
  45. 45. My project in academic style.
  46. 46. Styrofoam
  47. 47. Styrofoam Styrene monomer
  48. 48. Polystyrene Chemical formula is (C8H8)n Monomer styrene Thermoplastic Blowing agents
  49. 49. Polystyrene Chemical formula is (C8H8)n Monomer styrene Thermoplastic Blowing agents 500 years++ for degradation http://en.wikipedia.org/wiki/Polystyrene
  50. 50. Recycling & Incineration
  51. 51. Recycling & Incineration 1% turn back to polystyrene most of the polystyrene is converted into carbon dioxide, water vapor, and heat. "Ease of Disposal". Retrieved 2009-06-25
  52. 52. Photo by Napat Jaitui JSTP # 15
  53. 53. Styrene Monomer Carbon Monoxide Photo by Napat Jaitui JSTP # 15
  54. 54. Styrene Monomer Carbon Monoxide Photo by Napat Jaitui JSTP # 15 CARCINOGEN
  55. 55. c c c c c c c c
  56. 56. c c c c c c c c Bacteria nutrition ‣ Energy source ‣ Carbon source ‣ Nitrogen source ‣ Minerals ‣ Water ‣ Growth factors
  57. 57. Howcan I find the bacteria that able to degrade a styrofoam?
  58. 58. SOIL
  59. 59. SOIL
  60. 60. SOIL Landfill Landfill foam
  61. 61. Howcan I find the bacteria that able to degrade a styrofoam?
  62. 62. Howcan I find the bacteria that able to degrade a styrofoam?
  63. 63. The ability
  64. 64. The key to isolate the effective microbe is the degradability.
  65. 65. MSM Broth Bacteria selective broth
  66. 66. MSM Broth Bacteria selective broth
  67. 67. MSM broth ‣K2HPO4 ‣KH2PO4 ‣(NH4)2SO4 ‣MgSO4 ‣ FeSO4.2HO2 ‣MnCl2.4H2O ‣CoCl2.6H2O ‣CuCl2.2H2O ‣NiCl2.6H2O ‣Na2MoO4.2H2O ‣ZnSO4.7H2O ‣H3BO3 Sterile Styrofoam MSM Broth Bacteria selective broth
  68. 68. MSM Broth Bacteria selective broth
  69. 69. Shake in shaker for 1 month then inoculate to new fresh broth for sub culture.
  70. 70. Every week the solution in each flask was taken to the eppendorf then stored at 2C๐ for stop bacteria growth that use for monitor the changing of bacteria population.
  71. 71. MSM Broth Bacteria selective broth
  72. 72. MSM Broth Bacteria selective broth
  73. 73. MSM broth ‣K2HPO4 ‣KH2PO4 ‣(NH4)2SO4 ‣MgSO4 ‣ FeSO4.2HO2 ‣MnCl2.4H2O ‣CoCl2.6H2O ‣CuCl2.2H2O ‣NiCl2.6H2O ‣Na2MoO4.2H2O ‣ZnSO4.7H2O ‣H3BO3 Sterile Styrofoam MSM Broth Bacteria selective broth
  74. 74. MSM Broth Bacteria selective broth
  75. 75. MSM Broth Bacteria selective broth
  76. 76. MSM Broth Bacteria selective broth
  77. 77. MSM Broth Bacteria selective broth Foam in landfill Soil in landfill
  78. 78. Week
  79. 79. Week
  80. 80. Week Dominant species
  81. 81. Week Dominant species Diversity
  82. 82. Every week the solution in each flask was taken to the eppendorf then stored at 2C๐ for stop bacteria growth that use for monitor the changing of bacteria population.
  83. 83. DNA Replication : PCR (TopTaq Master Mix Kit) 16S rRNA gene Amplification by using Primer VR (Medlin et al., 1998) & VFC (Muyzer et al., 1993)
  84. 84. DNA Replication : PCR (TopTaq Master Mix Kit) 16S rRNA gene Amplification by using Primer VR (Medlin et al., 1998) & VFC (Muyzer et al., 1993)
  85. 85. DNA Replication : PCR (TopTaq Master Mix Kit) 16S rRNA gene Amplification by using Primer VR (Medlin et al., 1998) & VFC (Muyzer et al., 1993)
  86. 86. DGGE (Denature Gradient Gel Electrophoresis) 30% Urea + Formamine 60% Urea + Formamine
  87. 87. DGGE (Denature Gradient Gel Electrophoresis) - 30% Urea + Formamine 60% Urea + Formamine +
  88. 88. DGGE (Denature Gradient Gel Electrophoresis) 30% Urea + Formamine 60% Urea + Formamine
  89. 89. DGGE (Denature Gradient Gel Electrophoresis) 30% Urea + Formamine 60% Urea + Formamine
  90. 90. DGGE (Denature Gradient Gel Electrophoresis) 30% Urea + Formamine 60% Urea + Formamine
  91. 91. DGGE (Denature Gradient Gel Electrophoresis) 30% Urea + Formamine 60% Urea + Formamine GC rich
  92. 92. DGGE (Denature Gradient Gel Electrophoresis) 30% Urea + Formamine 60% Urea + Formamine GC low GC rich
  93. 93. DGGE (Denature Gradient Gel Electrophoresis) 30% Urea + Formamine 60% Urea + Formamine GC low GC rich
  94. 94. Marker Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Soilweek20 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Foamweek20 Controlweek6 Controlweek7 Controlweek8 Marker Marker Negative Bacteria from foamBacteria from soil Control DGGE 26/04/55 Running time 300 minute from PCR product 24/04/55 template use 8 µl. DGGE (Denature Gradient Gel Electrophoresis)
  95. 95. Marker Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Soilweek20 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Foamweek20 Controlweek6 Controlweek7 Controlweek8 Marker Marker Negative Bacteria from foamBacteria from soil Control DGGE 26/04/55 Running time 300 minute from PCR product 24/04/55 template use 8 µl. DGGE (Denature Gradient Gel Electrophoresis)
  96. 96. Marker Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Soilweek20 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Foamweek20 Controlweek6 Controlweek7 Controlweek8 Marker Marker Negative Bacteria from foamBacteria from soil Control DGGE 26/04/55 Running time 300 minute from PCR product 24/04/55 template use 8 µl. DGGE (Denature Gradient Gel Electrophoresis)
  97. 97. Marker Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Soilweek20 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Foamweek20 Controlweek6 Controlweek7 Controlweek8 Marker Marker Negative Bacteria from foamBacteria from soil Control DGGE 26/04/55 Running time 300 minute from PCR product 24/04/55 template use 8 µl. DGGE (Denature Gradient Gel Electrophoresis)
  98. 98. Marker Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Soilweek20 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Foamweek20 Controlweek6 Controlweek7 Controlweek8 Marker Marker Negative Bacteria from foamBacteria from soil Control DGGE 26/04/55 Running time 300 minute from PCR product 24/04/55 template use 8 µl. DGGE (Denature Gradient Gel Electrophoresis)
  99. 99. Marker Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Soilweek20 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Foamweek20 Controlweek6 Controlweek7 Controlweek8 Marker Marker Negative Bacteria from foamBacteria from soil Control DGGE 26/04/55 Running time 300 minute from PCR product 24/04/55 template use 8 µl. DGGE (Denature Gradient Gel Electrophoresis)
  100. 100. The microscopic structure of Polystyrene observed by SEM Pat Pataranutaporn1 , Apinya.J2 Assistant prof. Savaporn Supaphol3 , 1 PSU.Wittayanusorn school, 2 Scientific Equipment Center Prince of Songkla University 3 Kasetsart University
  101. 101. Control : Polystyrene in MSM broth without bacterial source. Regular polystyrene foam that didn’t use in experiment. 100x 200x 500x The comparison of Polystyrene microscopic structure Control
  102. 102. Regular polystyrene that didn’t use in experiment. Control
  103. 103. Polystyrene in Medium with bacteria from foam sample. 100x 200x 500x The comparison of Polystyrene microscopic structure Regular polystyrene foam that didn’t use in experiment. Polystyrene in Medium with bacteria from foam sample. Bacteria from foam
  104. 104. Polystyrene in Medium with bacteria from foam sample. 100x 200x 500x The comparison of Polystyrene microscopic structure Regular polystyrene foam that didn’t use in experiment. Bacteria from foam
  105. 105. Polystyrene in Medium with bacteria from soil sample. 100x 200x 500x The comparison of Polystyrene microscopic structure Regular polystyrene foam that didn’t use in experiment. Bacteria from soil
  106. 106. Polystyrene in Medium with bacteria from soil sample. 100x 200x 500x The comparison of Polystyrene microscopic structure Regular polystyrene foam that didn’t use in experiment.Polystyrene in Medium with bacteria from soil sample. Bacteria from soil
  107. 107. Polystyrene in Medium with bacteria from soil sample. 100x 200x 500x The comparison of Polystyrene microscopic structure Regular polystyrene foam that didn’t use in experiment.Polystyrene in Medium with bacteria from soil sample. Bacteria from soil
  108. 108. Polystyrene in Medium with bacteria from soil sample. 100x 200x 500x The comparison of Polystyrene microscopic structure Regular polystyrene foam that didn’t use in experiment.Polystyrene in Medium with bacteria from soil sample. Bacteria from soil
  109. 109. Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Controlweek6 Controlweek7 Controlweek8 Foam sampleSoil sampleControl
  110. 110. Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Controlweek6 Controlweek7 Controlweek8 Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Controlweek6 Controlweek7 Controlweek8 Foam sampleSoil sampleControl
  111. 111. Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Controlweek6 Controlweek7 Controlweek8 Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Controlweek6 Controlweek7 Controlweek8 Foam sampleSoil sampleControl
  112. 112. Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Controlweek6 Controlweek7 Controlweek8 Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Controlweek6 Controlweek7 Controlweek8 Foam sampleSoil sampleControl
  113. 113. Molecular cloning DNA isolation & DNA Analyzing Pat Pataranutaporn1 , Sureeporn Nualkaew2 , Prof. Dr. Amornrat Phongdara2 1 PSU.Wittayanusorn school, 2 Prince of Songkla University
  114. 114. Marker Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Soilweek20 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Foamweek20 Controlweek6 Controlweek7 Controlweek8 Marker Marker Negative Bacteria from foamBacteria from soil Control DGGE 26/04/55 Running time 300 minute from PCR product 24/04/55 template use 8 µl. DGGE (Denature Gradient Gel Electrophoresis)
  115. 115. Marker Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Soilweek20 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Foamweek20 Controlweek6 Controlweek7 Controlweek8 Marker Marker Negative Bacteria from foamBacteria from soil Control DGGE 26/04/55 Running time 300 minute from PCR product 24/04/55 template use 8 µl. DGGE (Denature Gradient Gel Electrophoresis)
  116. 116. Marker Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Soilweek20 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Foamweek20 Controlweek6 Controlweek7 Controlweek8 Marker Marker Negative Bacteria from foamBacteria from soil Control DGGE 26/04/55 Running time 300 minute from PCR product 24/04/55 template use 8 µl. DGGE (Denature Gradient Gel Electrophoresis) Hello Who are you ?
  117. 117. Molecular cloning Despicable Me: Minion Mayhem
  118. 118. PCR Product
  119. 119. PCR Product
  120. 120. PCR Product Mix DNA for ligation pGEM- T easy Target DNA
  121. 121. PCR Product Mix DNA for ligation
  122. 122. PCR Product Mix DNA for ligation
  123. 123. Transform plasmid to the competent cell.
  124. 124. E.Coli as Transform plasmid to the competent cell.
  125. 125. Transform plasmid to the competent cell.
  126. 126. Transform plasmid to the competent cell.
  127. 127. Transform plasmid to the competent cell.
  128. 128. Transform plasmid to the competent cell. The separate DNA
  129. 129. Marker Soilweek1 Soilweek5 Soilweek6 Soilweek7 Soilweek8 Soilweek20 Foamweek1 Foamweek5 Foamweek6 Foamweek7 Foamweek8 Foamweek20 Controlweek6 Controlweek7 Controlweek8 Marker Marker Negative Bacteria from foamBacteria from soil Control DGGE 26/04/55 Running time 300 minute from PCR product 24/04/55 template use 8 µl. DGGE (Denature Gradient Gel Electrophoresis)
  130. 130. Electropherogram Code name : Control 4
  131. 131. Code name : Control 4 Basic Local Alignment Search Tool (BLAST) Database Name TL/16S_ribosomal_RNA_Bacteria_and_Archaea Description 16S ribosomal RNA sequences (Bacteria and Archaea) Program BLASTN 2.2.27+ Molecule type nucleic acid Query Length 1055
  132. 132. Code name : Control 4 Basic Local Alignment Search Tool (BLAST) Database Name TL/16S_ribosomal_RNA_Bacteria_and_Archaea Description 16S ribosomal RNA sequences (Bacteria and Archaea) Program BLASTN 2.2.27+ Molecule type nucleic acid Query Length 1055
  133. 133. Code name : Control 4 Phylogenetic tree
  134. 134. Code name : Control 4 Phylogenetic tree
  135. 135. Code name : Control 4 Phylogenetic tree
  136. 136. Electropherogram Code name : Control 7
  137. 137. Code name : Control 7 Basic Local Alignment Search Tool (BLAST) Database Name TL/16S_ribosomal_RNA_Bacteria_and_Archaea Description 16S ribosomal RNA sequences (Bacteria and Archaea) Program BLASTN 2.2.27+ Molecule type nucleic acid Query Length 1006
  138. 138. Code name : Control 7 Basic Local Alignment Search Tool (BLAST) Database Name TL/16S_ribosomal_RNA_Bacteria_and_Archaea Description 16S ribosomal RNA sequences (Bacteria and Archaea) Program BLASTN 2.2.27+ Molecule type nucleic acid Query Length 1006
  139. 139. Code name : Control 7 Phylogenetic tree
  140. 140. Code name : Control 7 Phylogenetic tree
  141. 141. Code name : Control 7 Phylogenetic tree
  142. 142. Code name : Fe5
  143. 143. Bacterial plating Plate streaking & Plate spreading Pat Pataranutaporn1 , Panwong Kuntanawat2 1 PSU.Wittayanusorn school, 2 King Mongkut's University of Technology Thonburi
  144. 144. Bacterial plating on MSM & MSM + PSA agar cultured at 37o C over 48 hr.
  145. 145. Bacterial plating on MSM & MSM + PSA agar cultured at 37o C over 48 hr.
  146. 146. Bacterial plating on MSM & MSM + PSA agar cultured at 37o C over 48 hr. Polystyrene-coacrylic acid (PSA) (particles diameter 500 nm) MSM Agar
  147. 147. Bacterial plating on MSM & MSM + PSA agar cultured at 37o C over 48 hr. Polystyrene-coacrylic acid (PSA) (particles diameter 500 nm) MSM Agar Polysaccharide Cx(H2O)y
  148. 148. Bacterial plating on MSM & MSM + PSA agar cultured at 37o C over 48 hr.
  149. 149. Bacterial plating on MSM & MSM + PSA agar cultured at 37o C over 48 hr.
  150. 150. Bacterial plating on MSM & MSM + PSA agar cultured at 37o C over 48 hr.
  151. 151. Bacterial plating on MSM & MSM + PSA agar cultured at 37o C over 48 hr.
  152. 152. Research Achievements
  153. 153. Research Achievements
  154. 154. Research Achievements 13th NCSC, Jaipur India 2011 Youth summit 2012, Dubai UAE JSTP Scholarship STT 36 BYEE, Leverkuzen Germany BYEE Poster Prize from india
  155. 155. Publication & media
  156. 156. JSTPMedia www.jstpmedia.org
  157. 157. Biodegradation of Polystyrene Foam by the Microorganisms from Landfill
  158. 158. P.P. TOYs
  159. 159. P.P. TOYs
  160. 160. หนังตะลุง
  161. 161. หนังตะลุง WEB app
  162. 162. หนังตะลุง WEB app Culture
  163. 163. Developed in SWF format http://jstpmedia.org/images/complete.swf
  164. 164. A S ◀ ◀ ◀ ◀ move to the next character Head(Speak) Left hand right hand Body flip
  165. 165. Demo
  166. 166. JSTP 15/3
  167. 167. P.P. TOYs
  168. 168. P.P. TOYs
  169. 169. Space
  170. 170. Space Gravity
  171. 171. Space Enzyme Gravity
  172. 172. JAXA
  173. 173. v
  174. 174. v
  175. 175. The Evaluation of Amylase Activity under Microgravity By JSTP 12 Pat Pataranutaporn Pondet Ananchai Arnan Schuett
  176. 176. ChemistryBiotechnology Electronic Research Team JSTP 12
  177. 177. ChemistryBiotechnology Electronic Research Team JSTP 12 The youngest team
  178. 178. Possibility of Enzyme
  179. 179. Possibility of Enzyme Collision Theory
  180. 180. Possibility of Enzyme Collision Theory ‣Enzyme Concentration ‣Substrate Concentration ‣Inhibitors ‣Temperature ‣pH
  181. 181. Gravity
  182. 182. GravityFree motion of molecule
  183. 183. Salivary stress markers and psychological stress in simulated microgravity: 21 days in 6° head-down tilt. Rai B, Kaur J. Catholic University Leuven, Belgium. Spaceflight occurs in an environment of temperature extremes, microgravity, solar and galactic cosmic radiation, lack of atmospheric pressure, and high-speed micrometeorites. Exposure to microgravity and the space environment during space missions of short and long duration has important medical and health implications for astronauts. Psychological well-being is of increasing importance in planned spaceflights and interplanetary missions of long duration. The 6° head-down tilt (HDT) is an established method of mimicking low gravity on earth. The aim of the present study was to determine the effects of 21 days of HDT on psychological stress in 12 healthy male volunteers. Psychological state was assessed by the current stress test, and chromogranin-A (CgA), cortisol, alpha-amylase, and beta-endorphin were measured in saliva. After one week of HDT, all volunteers developed psychological stress, and secretion of CgA, cortisol, alpha-amylase, and beta-endorphin were all significantly higher. Thus, 6° HDT appears to be a valid model to induce psychological stress changes in the immune system, changes that might also be encountered by astronauts and cosmonauts during both a short stay in space, such as that required while orbiting a space station, and in longer spaceflights. Adaptation mechanism
  184. 184. Difference enzyme activity rate affected by gravity
  185. 185. Enzyme containing
  186. 186. Enzyme containing Pumping system
  187. 187. Enzyme containing Pumping system Starch measuring
  188. 188. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring
  189. 189. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring
  190. 190. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring 2
  191. 191. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring 24 2
  192. 192. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring 246 2 24
  193. 193. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring 2468 2 2 24 46
  194. 194. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring 2468 2 2 2 04 46
  195. 195. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring 2468 2 2 2 04 46
  196. 196. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring 2468 2 2 2 04 46
  197. 197. EnzymeamylaseStarchAcedicacid Enzyme containing Pumping system Starch measuring 2468 2 2 2 04 46 low starch remain High starch remain
  198. 198. 026 48
  199. 199. 026 48
  200. 200. starch remain (%v/w) 026 48
  201. 201. starch remain (%v/w) Time (s) 026 48
  202. 202. starch remain (%v/w) Time (s) 026 48
  203. 203. starch remain (%v/w) Time (s) 026 48
  204. 204. starch remain (%v/w) Time (s) 026 48
  205. 205. starch remain (%v/w) Time (s) 026 48 Y
  206. 206. starch remain (%v/w) Time (s) 026 48 Y X
  207. 207. starch remain (%v/w) Time (s) 026 48 Y X Rate =
  208. 208. Working period
  209. 209. Working period 2 moths for discussing
  210. 210. Working period 2 moths for discussing 3 hours for proposal
  211. 211. Working period 2 moths for discussing 3 hours for proposal 1 night for presentation
  212. 212. Working period 2 moths for discussing 3 hours for proposal 1 night for presentation 5 minutes for presentation rehearsing
  213. 213. JSTP Team JSTP Team
  214. 214. ไม่มี limit ชีวิตเกินร้อย
  215. 215. PP’s TOYP.P. TOYs
  216. 216. PP’s TOYP.P. TOYs
  217. 217. What you are seeing now.
  218. 218. Presentation DESCIPost modern interface
  219. 219. Available at JSTPMedia soon !
  220. 220. !
  221. 221. You have a chance to answer your own question .
  222. 222. ของเล่นที่สนุก เราต้องเลือกเอง
  223. 223. “ Never be afraid to asked “
  224. 224. POSSIBLEIM
  225. 225. AKE POSSIBLEI M
  226. 226. Right now...
  227. 227. It’s your turn
  228. 228. It’s your turn
  229. 229. THANK YOU

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